BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0016.p (240 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr2045 [Q] COG3208 Predicted thioesterase involved in non-ribos... 160 2e-39 PA2411 [Q] COG3208 Predicted thioesterase involved in non-riboso... 134 1e-31 PA4229 [Q] COG3208 Predicted thioesterase involved in non-riboso... 127 1e-29 PA2425 [Q] COG3208 Predicted thioesterase involved in non-riboso... 125 7e-29 YPO0773 [Q] COG3208 Predicted thioesterase involved in non-ribos... 108 9e-24 >alr2045 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 359 Score = 160 bits (404), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 15/231 (6%), Positives = 41/231 (17%), Gaps = 5/231 (2%) Query: 10 GMKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRF-VEPFYPSLHDGVMDIST 68 + A A + E + R + V+ + Sbjct: 22 ELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAG-GITKPVLLLEG 80 Query: 69 AIMDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQD-KKKIHH 127 + S +L +A+ E + ++ Sbjct: 81 FFDARD-LPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEA 139 Query: 128 LPDDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVL 187 + +R + + + N Q +L Sbjct: 140 FYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGK-PGQRSIAASGGIL 198 Query: 188 VGDEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTL 238 + + + + G + +I + Sbjct: 199 LWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKA 249 >PA2411 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 134 bits (337), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 73/228 (32%), Positives = 118/228 (51%), Gaps = 5/228 (2%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 ++LFCLPY+G SA +++W+R+L + + + PVEL GRG R EP L ++ + Sbjct: 6 VRLFCLPYSGASAMTYSRWRRKLPAWLAVRPVELPGRGARMAEPLQTDLASLAQQLAREL 65 Query: 71 MDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQ---DKKKIHH 127 D++ Y++ GHS+G L CE++ ++ G P F G P + D+ Sbjct: 66 HDEVRQGPYAMLGHSLGALLACEVLYALRELGCPTPLGFFACGTAAPSRRAEYDRGFAEP 125 Query: 128 LPDDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVL 187 D E ++ L GTP++V N+ELM + P+LR+D+ SY + + I L Sbjct: 126 KSDAELIADLRDLQGTPEEVLGNRELMSLTLPILRADFLLCGSYRHQR-RPPLACPIRTL 184 Query: 188 VGDEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLIN 235 G ED+ S + W E T + F GGHFFI++ E +V+ ++ Sbjct: 185 GGREDK-ASEEQLLAWAEETRSGFELELFDGGHFFIHQREAEVLAVVE 231 >PA4229 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 251 Score = 127 bits (320), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 71/228 (31%), Positives = 117/228 (51%), Gaps = 4/228 (1%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 ++L C P+AGGSA+ F W L I+L+ ++ GR RF E L D + D + Sbjct: 17 LRLACFPHAGGSASFFRSWSERLPPDIDLLALQYPGREDRFNEAPATRLED-LADGAALA 75 Query: 71 MDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQDKKKIHHLPD 130 + D +LFGHS+G E ++++ G RHLF+S PH Q +H + Sbjct: 76 LRDFADAPLALFGHSLGAALAYETALRLESAGAPL-RHLFVSAHPAPHRQRGGALHRGDE 134 Query: 131 DEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLVGD 190 ++V R GG ++ E+ +L +F P+LR+DY+ +E+Y P + VL+G+ Sbjct: 135 AALLEDVRRQGGA-SELLEDADLRALFLPILRADYQAIETYRRAQPI-ALACALDVLLGE 192 Query: 191 EDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTL 238 DE S + + W++ + + F GGHF+++E VI+ + R L Sbjct: 193 HDEEVSAAEAQAWSDASRTPARLRRFPGGHFYLSEGRDAVIEHLLRRL 240 >PA2425 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 125 bits (314), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 73/228 (32%), Positives = 113/228 (49%), Gaps = 4/228 (1%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 ++LFCLPY+G SA +F +W+ L I L+P+EL GRG RF E + V ++ + Sbjct: 7 LQLFCLPYSGASARLFRRWQPSLPGWIRLLPLELPGRGLRFGEALHTDAASLVAQLAGEL 66 Query: 71 MDQIGDKG-YSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQDKKKIHHLP 129 + Y+ FGHS+G L EL ++ G E P LFLSG+ PP D Sbjct: 67 QGTLDSASPYAFFGHSLGGLLAFELAHAMRRRGLEGPLALFLSGVSPPAENDVSGYRRAK 126 Query: 130 DDEFWQEVIR-LGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLV 188 D +R GTP+ V + LM++ PV+R+D+ SY +A D + + Sbjct: 127 ADAELVAALRDYRGTPEAVLNDPSLMQMLLPVVRADFLVTGSYRYQA-HGPLDAALHLFG 185 Query: 189 GDEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINR 236 G ED + S ++ W ++ G HFFI E++ +++ L+ R Sbjct: 186 GREDSLRS-AELLGWLHEAGGDFTLDLLDGQHFFIREQQGQLLRLLRR 232 >YPO0773 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 108 bits (269), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 62/229 (27%), Positives = 111/229 (48%), Gaps = 4/229 (1%) Query: 12 KLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAIM 71 ++ C +AGGSA F WK +L L ++L G G R E +LH + D+ Sbjct: 16 QMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQ-MADVIAQSY 74 Query: 72 DQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQDKKKIHHLPDD 131 + + FGHSMG E+ ++Q G L++SG PPH + D Sbjct: 75 VYLPVRDTFFFGHSMGAALAFEVALRLQKAG-RGITTLYVSGRLPPHKNTNTDFYRQSDV 133 Query: 132 EFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLVGDE 191 ++ +LGG+ + EL ++ P+ R+D+ +E+Y + E + + VG++ Sbjct: 134 SILNKITQLGGSTLDY-QQPELKDLLLPIFRADFTAIETYQRQCT-ELLNCSVMACVGEQ 191 Query: 192 DEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTLIA 240 D S+ + +W + T + + F GGHF++N +K+++ IN+++ A Sbjct: 192 DTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVAA 240 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,501,712 Number of extensions: 902618 Number of successful extensions: 1217 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1189 Number of HSP's successfully gapped: 11 Length of query: 240 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 137 Effective length of database: 26,499,029 Effective search space: 3630366973 Effective search space used: 3630366973 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)