BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0014.p (218 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2930 [R] COG0637 Predicted phosphatase/phosphohexomutase 84 1e-16 BH3587 [R] COG0546 Predicted phosphatases 77 2e-14 PA0608 [R] COG0546 Predicted phosphatases 73 3e-13 PA2974 [R] COG0546 Predicted phosphatases 72 8e-13 RSc1042 [R] COG0546 Predicted phosphatases 71 1e-12 >lin2930 [R] COG0637 Predicted phosphatase/phosphohexomutase Length = 346 Score = 84.3 bits (207), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 8/205 (3%), Positives = 27/205 (13%), Gaps = 15/205 (7%) Query: 1 MNNAVVFDFDGVIIDSLEVQRKAFFESFRLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLS 60 M + + ++ + L + G + + ++ Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQL-DYAVAQGINLIDVAEMYPVPPRP 59 Query: 61 LDMTEHYRRIARE-------NIHLIQIIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTL 113 + +I Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 114 GEHGLAGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKM 173 V T+ + ++ + + + Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 174 RSIAVAWGHNPVEILRQE--APSYV 196 A V Sbjct: 180 NETAF-----GVMRYLHLADKHDLP 199 >BH3587 [R] COG0546 Predicted phosphatases Length = 346 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 14/206 (6%), Positives = 29/206 (14%), Gaps = 8/206 (3%) Query: 3 NAVVFDFDGVIIDSLEVQRKAFFESFRLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLSLD 62 + + V L S + D + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLD-YAVAQGINLIDVAEMYPVP--PRPE 60 Query: 63 MTEHYRRIARENIH-----LIQIIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTLGEHG 117 + II D G + R L Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 118 LAGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIA 177 L + + P L + V + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 178 VAWGHNPVEILRQEAPSYVAYNVEEL 203 + + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQN 206 >PA0608 [R] COG0546 Predicted phosphatases Length = 346 Score = 73.2 bits (178), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 8/216 (3%), Positives = 27/216 (12%), Gaps = 23/216 (10%) Query: 5 VVFDFDGVIIDS---LEVQRKAFFESFRLVSDGPLPSFEEFLGHSG-------------- 47 + + + + + + + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLID-VAEMYPVPPRPETQGLTETYVGNWLA 74 Query: 48 DSIPNIFTKMGLSLDMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGKDRP 107 + + + + + +R D Sbjct: 75 KHGSREKLIIASKVSGPSRN-----NDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLY 129 Query: 108 RTLLTLGEHGLAGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIIC 167 + G L + V + Sbjct: 130 QVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRY 189 Query: 168 AREAKMRSIAVAWGHNPVEILRQEAPSYVAYNVEEL 203 A + L + V + Sbjct: 190 LHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQY 225 >PA2974 [R] COG0546 Predicted phosphatases Length = 346 Score = 71.6 bits (174), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 8/223 (3%), Positives = 24/223 (10%), Gaps = 34/223 (15%) Query: 5 VVFDFDGVIIDSLEVQRKAFFESFRLVSDGPLPSFEEFLGHSGDS-------------IP 51 + + + + + +P Sbjct: 7 PHSSLEVSTLGLGT----------MTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVP 56 Query: 52 NIFTKMG-------LSLDMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGK 104 G L D Sbjct: 57 PRPETQGLTETYVGNWLAKHGSR--EKLII-ASKVSGPSRNNDKGIRPDQALDRKNIREA 113 Query: 105 DRPRTLLTLGEHGLAGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKND 164 ++ + K ++ Sbjct: 114 LHDSLKRLQTDYLDLYQVHWPQRPTN-CFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGK 172 Query: 165 IICAREAKMRSIAVAWGHNPVEILRQEAPSYVAYNVEELRHGL 207 I + + V + + + L Sbjct: 173 IRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSF 215 >RSc1042 [R] COG0546 Predicted phosphatases Length = 346 Score = 70.9 bits (172), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 10/211 (4%), Positives = 30/211 (14%), Gaps = 8/211 (3%) Query: 3 NAVVFDFDGVIIDSLEVQRKAFFESFRLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLSLD 62 + + + ++ + + L + P L Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQL-DYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 63 MTEHYRRIARENIHLIQ------IIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTLGEH 116 T +A+ D ++ Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 117 GLAGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSI 176 + K ++ I + + Sbjct: 126 LDLYQVHWPQRPTN-CFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 177 AVAWGHNPVEILRQEAPSYVAYNVEELRHGL 207 V + + + L Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSF 215 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,120,145 Number of extensions: 740585 Number of successful extensions: 1405 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 1383 Number of HSP's successfully gapped: 49 Length of query: 218 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 116 Effective length of database: 26,614,363 Effective search space: 3087266108 Effective search space used: 3087266108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)