BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0011.p (1018 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all2644_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 571 e-162 BS_pksK_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 519 e-147 AGl2309_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 498 e-140 RSp0641_7 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 472 e-132 RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 460 e-129 >all2644_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 571 bits (1471), Expect = e-162, Method: Compositional matrix adjust. Identities = 0/831 (0%), Positives = 0/831 (0%), Gaps = 35/831 (4%) Query: 4 DTSRRFKQWLKTEGVTLNMGLLALYAVVLFKICQQETVIIGTPVSGRKHTDLEHVIGPFV 63 + L + + I ++ + Sbjct: 249 AKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLG 308 Query: 64 DTVALKLSMGAQASFAEFLQEVKQTLSSALDHQDYPFEELIGSLDLEQDLSRNPLFETMF 123 Sbjct: 309 368 Query: 124 SYREVYDKTNQIGGVREITSFYRHKTAMFTIRFTAWEEGPQIGCEIEYSTTRLAQETMQR 183 Sbjct: 369 428 Query: 184 FAGFFEHLMQQVLNGPARPLAEIAVMTESEKQQILFGFNQTQSIVEPGQNIVSLFERQAE 243 Sbjct: 429 -- 486 Query: 244 EHPEKIAVAAGLTAMSYLELNHAVNQLAHYLQKNGVTRGTPVGIYLERSPDAAVAILAVL 303 Sbjct: 487 546 Query: 304 KAGGCYVPVDPSIPIRRILQIQEEAGLVVMLSTSDLTEQAKL-------------ECGRM 350 Sbjct: 547 606 Query: 351 ILLDREREAILQESSANPGNGV-EASHTAYMIFTSGSTGKPKGVKIPHRALTNFLLSMAK 409 Sbjct: 607 -- 664 Query: 410 KPGMTAEDKLLSVTTLSFDIFGLELFLPLVTGALVVLATNREAADGRVLGQLLAAHGITV 469 Sbjct: 665 724 Query: 470 MQATPATWKMXXXXXXXXXXXXKALCGGESLPPDVARELLPKVGSLWNMYGPTETTIWST 529 Sbjct: 725 784 Query: 530 VAEITNAGQILIGKPIDNTQIYILDQNRNPVPLGVYGEMYIGGAGVADGYVNHQQLTEQS 589 Sbjct: 785 844 Query: 590 FVD-----NPFSTLYPKMYRTGDLARFLPDGTLQCEGRMDDQVKLRGFRIELSEIQAVIA 644 Sbjct: 845 904 Query: 645 AYPGIKDNVVVVESGLPNGEEPESDKKSIISYMVPAGPEAVHIDELKSYLLERLPSYMIP 704 Sbjct: 905 -------- 956 Query: 705 VQFFIIDQIPLSANQKTDKKKLRTMQQKLLVSAKAYKEPVSELEKQISSIWMKLLHKETI 764 Sbjct: 957 ---- 1012 Query: 765 SITDSYFDLGGTSITVVLLQEQMREAFGVPLQVADIFAHPTIERLAGFMER 815 Sbjct: 1013 1063 >BS_pksK_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 519 bits (1337), Expect = e-147, Method: Compositional matrix adjust. Identities = 0/827 (0%), Positives = 0/827 (0%), Gaps = 32/827 (3%) Query: 10 KQWLKTEGVTLNMGLLALYAVVLFKICQQETVIIGTPVSGRKHTDLEHVIGPFVDTVALK 69 Sbjct: 330 389 Query: 70 LSMGAQASFAEFLQEVKQTLSSALDHQDYPFEELIGSLDLEQDLSRNPLFETMFSYREVY 129 Sbjct: 390 449 Query: 130 DKTNQIGGVREITSF-------YRHKTAMFTIRFTAWEEGPQIGCEIEYSTTRLAQETMQ 182 Sbjct: 450 509 Query: 183 RFAGFFEHLMQQVLNGPARPLAEIAVMTESEKQQILFGFNQTQSIVEPGQNIVSLFERQA 242 Sbjct: 510 - 568 Query: 243 EEHPEKIAVAAGLTAMSYLELNHAVNQLAHYLQKNGVTRGTPVGIYLERSPDAAVAILAV 302 Sbjct: 569 628 Query: 303 LKAGGCYVPVDPSIPIRRILQIQEEAGLVVMLSTSDLTEQAKLECGRMILLDREREAILQ 362 Sbjct: 629 688 Query: 363 ESSANP--GNGVEASHTAYMIFTSGSTGKPKGVKIPHRALTNFLLSMAKKPGMTAEDKLL 420 Sbjct: 689 748 Query: 421 SVTTLSFDIFGLELFLPLVTGALVVLATNREAADGRVLGQLLAAHGITVMQATPATWKMX 480 Sbjct: 749 808 Query: 481 XXXXXXXXXXXKALCGGESLPPDVARELLPKVGSLWNMYGPTETTIWSTVAEIT-NAGQI 539 Sbjct: 809 868 Query: 540 LIGKPIDNTQIYILDQNRNPVPLGVYGEMYIGGAGVADGYVNHQQLTEQSFVDNPFSTLY 599 Sbjct: 869 - 927 Query: 600 PKMYRTGDLARFLPDGTLQCEGRMDDQVKLRGFRIELSEIQAVIAAYPGIKDNVVVVESG 659 Sbjct: 928 ---- 983 Query: 660 LPNGEEPESDKKSIISYMVPAGPEA-VHIDELKSYLLERLPSYMIPVQFFIIDQIPLSAN 718 Sbjct: 984 -------- 1035 Query: 719 QKTDKKKLRTMQQKLLVSAKAYKEPVS---ELEKQISSIWMKLLHKETISITDSYFDLGG 775 Sbjct: 1036 ---- 1091 Query: 776 TSITVVLLQEQMREAFGVPLQVADIFAHPTIERLAGFMERKMTPEAS 822 Sbjct: 1092 1138 >AGl2309_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 498 bits (1281), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/821 (0%), Positives = 0/821 (0%), Gaps = 36/821 (4%) Query: 10 KQWLKTEGVTLNMGLLALYAVVLFKICQQETVIIGTPVSGRKHTDLEHVIGPFVDTVALK 69 E + A Q + T L + T Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIES 321 Query: 70 LSMGAQASFAEFLQEVKQTLSSALDHQDYPFEELIGSLDLEQDLSRNPLFETMFSYREVY 129 Sbjct: 322 381 Query: 130 DKTNQIGGVREITSFYRHKTAMFTIRFTAWEEGPQIGCE--IEYSTTRLAQETMQRFAGF 187 Sbjct: 382 441 Query: 188 FEHLMQQVLNGPARPLAEIAVMTESEKQQILFGFNQTQSIVEPGQNIVSLFERQAEEHPE 247 Sbjct: 442 -- 499 Query: 248 KIAVAAGLTAMSYLELNHAVNQLAHYLQKN-GVTRGTP-VGIYLERSPDAAVAILAVLKA 305 Sbjct: 500 559 Query: 306 GGCYVPVDPSIPI-RRILQIQEEAGLVVMLSTSDLTEQAKLECGRMILLDREREAILQES 364 Sbjct: 560 -------- 611 Query: 365 SA--NPGNGVEASHTAYMIFTSGSTGKPKGVKIPHRALTNFLLSMAKKPGMTAEDKLLSV 422 Sbjct: 612 671 Query: 423 TTLSFDIFGLELFLPLVTGALVVLATNREAADGRVLGQLLAAHGITVMQATPATWKMXXX 482 Sbjct: 672 731 Query: 483 XXXXXXXXXKALCGGESLPPDVARELLPKVGSLWNMYGPTETTIWSTVAEITNAGQIL-- 540 Sbjct: 732 791 Query: 541 --IGKPIDNTQIYILDQNRNPVPLGVYGEMYIGGAGVADGYVNHQQLTEQSFVDNPFSTL 598 Sbjct: 792 851 Query: 599 -----YPKMYRTGDLARFLPDGTLQCEGRMDDQVKLRGFRIELSEIQAVIAAYPGIKDNV 653 Sbjct: 852 911 Query: 654 VVVESGLPNGEEPESDKKSIISYMVPAGPEAVHIDELKSYLLERLPSYMIPVQFFIIDQI 713 Sbjct: 912 -------- 963 Query: 714 PLSANQKTDKKKLRTMQQKLLVSAKAYKEPVSELEKQISSIWMKLLHKETISITDSYFDL 773 Sbjct: 964 -- 1021 Query: 774 GGTSITVVLLQEQMREAFGVPLQVADIFAHPTIERLAGFME 814 Sbjct: 1022 1062 >RSp0641_7 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 472 bits (1215), Expect = e-132, Method: Compositional matrix adjust. Identities = 0/821 (0%), Positives = 0/821 (0%), Gaps = 28/821 (3%) Query: 2 DADTSRRFKQWLKTEGVTLNMGLLALYAVVLFKICQQETVIIGTPVSGRKHTDLEHVIGP 61 K G + ++T Sbjct: 232 AYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQM 291 Query: 62 FVDTVALKLSMGAQASFAEFLQEVKQTLSSALDHQDYPFEELIGSLDLEQDLSRNPLFET 121 + V + + + Sbjct: 292 ALAFVRRQPFVAS- 350 Query: 122 MFSY--REVYDKTNQIGGVREITSFYRHKTAMFTIRFTAWEEGPQIGCEIEYSTTRLAQE 179 Sbjct: 351 410 Query: 180 TMQRFAGFFEHLMQQVLNGPARPLAEIAVMTESEKQQILFGFNQTQSIVEPGQNIVSLFE 239 Sbjct: 411 470 Query: 240 RQAEEHPEKIAVAAGLTAMSYLELNHAVNQLAHYLQKNGVTRGTPVGIYLERSPDAAVAI 299 Sbjct: 471 530 Query: 300 LAVLKAGGCYVPVDPSIPIRRILQIQEEAGLVVMLSTSDLTEQAKLECGRMILLDREREA 359 Sbjct: 531 ---- 586 Query: 360 ILQESSANPG-NGVEASHTAYMIFTSGSTGKPKGVKIPHRALTNFLLSMAKKPGMTAEDK 418 Sbjct: 587 646 Query: 419 LLSVTTLSFDIFGLELFLPLVTGALVVLATNREAADGRVLGQLLAAHGITVMQATPATWK 478 Sbjct: 647 706 Query: 479 --MXXXXXXXXXXXXKALCGGESLPPDVARELLPKV--GSLWNMYGPTETTI----WSTV 530 Sbjct: 707 766 Query: 531 AEITNAGQILIGKPIDNTQIYILDQNRNPVPLGVYGEMYIGGAGVADGYVNHQQLTEQSF 590 Sbjct: 767 - 825 Query: 591 VDNPF-STLYPKMYRTGDLARFLPDGTLQCEGRMDDQVKLRGFRIELSEIQAVIAAYPGI 649 Sbjct: 826 885 Query: 650 KDNVVVVESGLPNGEEPESDKKSIISYMVPAGPEAVHIDELKSYLLERLPSYMIPVQFFI 709 Sbjct: 886 --------- 936 Query: 710 IDQIPLSANQKTDKKKLRTMQQKLLVSAKAYKEPVSELEKQISSIWMKLLHKETISITDS 769 Sbjct: 937 - 995 Query: 770 YFDLGGTSITVVLLQEQMREAFGVPLQVADIFAHPTIERLA 810 Sbjct: 996 1036 >RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 460 bits (1184), Expect = e-129, Method: Compositional matrix adjust. Identities = 0/810 (0%), Positives = 0/810 (0%), Gaps = 17/810 (2%) Query: 2 DADTSRRFKQWLKTEGVTLNMGLLALYAVVLFKICQQETVIIGTPVSGRKHTDLEHVIGP 61 K G + ++T Sbjct: 232 AYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQM 291 Query: 62 FVDTVALKLSMGAQASFAEFLQEVKQTLSSALDHQDYPFEELIGSLDLEQDLSRNPLFET 121 + V + + + Sbjct: 292 ALAFVRRQPFVAS- 350 Query: 122 MFSYREVYDKTNQIGGVREITSFYRHKTAMFTIRFTAWEEGPQIGCEIEYSTTRLAQETM 181 Sbjct: 351 410 Query: 182 QRFAGFFEHLMQQVLNGPARPLAEIAVMTESEKQQILFGFNQTQSIVEPGQNIVSLFERQ 241 Sbjct: 411 - 469 Query: 242 AEEHPEKIAVAAGLTAMSYLELNHAVNQLAHYLQKNGVTRGTPVGIYLERSPDAAVAILA 301 Sbjct: 470 529 Query: 302 VLKAGGCYVPVDPSIPIRRILQIQEEAGLVVMLSTSDLTEQAKLECGRMILLDREREAIL 361 Sbjct: 530 589 Query: 362 QESSANPGNGVEASHTAYMIFTSGSTGKPKGVKIPHRALTNFLLSMAKKPGMTAEDKLLS 421 Sbjct: 590 649 Query: 422 VTTLSFDIFGLELFLPLVTGALVVLATNREAADGRVLGQLLAAHGITVMQATPATWKMXX 481 Sbjct: 650 -- 707 Query: 482 XXXXXXXXXXKALCGGESLPPDVARELLPKVGSLWNMYGPTETTIWSTVAE-ITNAGQIL 540 Sbjct: 708 - 766 Query: 541 IGKPIDNTQIYILDQNRNPVPLGVYGEMYIGGAGVADGYVNHQQLTEQSFVDNPFSTLYP 600 Sbjct: 767 - 825 Query: 601 KMYRTGDLARFLPDGTLQCEGRMDDQVKLRGFRIELSEIQAVIAAYPGIKDNVVVVESGL 660 Sbjct: 826 885 Query: 661 PNGEEPESDKKSIISYMVPAGPEAVHIDELKSYLLERLPSYMIPVQFFIIDQIPLSANQK 720 Sbjct: 886 --------- 936 Query: 721 TDKKKLRTMQQKLLVSAKAYKEPVSELEKQISSIWMKLLHKETISITDSYFDLGGTSITV 780 Sbjct: 937 - 995 Query: 781 VLLQEQMREAFGVPLQVADIFAHPTIERLA 810 Sbjct: 996 1025 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 72,459,803 Number of extensions: 2992709 Number of successful extensions: 8506 Number of sequences better than 1.0e-05: 229 Number of HSP's gapped: 7997 Number of HSP's successfully gapped: 243 Length of query: 1018 Length of database: 38,378,431 Length adjustment: 116 Effective length of query: 902 Effective length of database: 24,999,687 Effective search space: 22549717674 Effective search space used: 22549717674 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)