BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0010.p (425 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 208 2e-53 BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 203 4e-52 YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 179 8e-45 BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 176 9e-44 BS_pksC [I] COG0331 (acyl-carrier-protein) S-malonyltransferase 169 1e-41 >CAC3575 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 308 Score = 208 bits (529), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 116/300 (38%), Positives = 175/300 (58%), Gaps = 2/300 (0%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M KIA +F GQGSQY MGK LY++ A+E F + +++LG+ + + EG ++L TE Sbjct: 1 MGKIAFVFSGQGSQYVGMGKDLYDNYQSAKETFDKADEVLGFKISELCFEGKDEELNLTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 QPA+ S A +++ + K P +AG SLGE +A C+G F++E A+RLV +R Sbjct: 61 NTQPAVLITSIAALRALEEEKGIKPDVVAGLSLGEYSAHVCSGSFSFEDAVRLVKKRGRY 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ+A +G G MAAI GL +V CA S E + VANYN Q+VI+G V A Sbjct: 121 MQEAVPKGIGTMAAIIGLEGDVVRGICAEQSK-EGIVEVANYNCPGQIVIAGEVKAVESA 179 Query: 181 AENLAKIGA-ISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSD 239 L + GA ++ L VS PFHT L+ + + + L ++ + PV++NV D Sbjct: 180 CTKLKESGARRTVMLSVSGPFHTSMLKSAAEKLEEELKNINIEDMKVPVITNVTGDYVED 239 Query: 240 PEGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEE 299 + I+ L Q++S+VRW++TI ++ GV+TFIELGP L++ IK+ + F+ E+ Sbjct: 240 KDQIKGLLKKQVMSSVRWEDTIRRMMDDGVDTFIELGPGKTLSSFIKKINRKMTIFNIEK 299 >BS_fabD [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 317 Score = 203 bits (517), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 111/288 (38%), Positives = 174/288 (60%), Gaps = 2/288 (0%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M KIA LFPGQGSQ+ MGK LYE A+ +F ++ L L ++I EG+ ++LT T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPA+ S A+ + +K P F AGHSLGE +AL AG +++ A+ V +R Sbjct: 61 NAQPALLTTSIAVLEK-FKESGITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGEF 119 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 M +A G+G MAAI G+ +++ +++ + + + +AN N Q+VISG+ V A Sbjct: 120 MNEAVPAGEGAMAAILGMDAEALKQVTDKVTEEGNLVQLANLNCPGQIVISGTAKGVELA 179 Query: 181 AENLAKIGAI-SIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSD 239 +E + GA +IPL+VS PFH+ ++ + + + VL A D+ + PV+SNV A ++ Sbjct: 180 SELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSADVMTE 239 Query: 240 PEGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKR 287 I+ +L Q+ S VR++E+I+ L+ +GV TFIE+GP L+ L+K+ Sbjct: 240 KADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVKK 287 >YPO1598 [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 309 Score = 179 bits (454), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 111/290 (38%), Positives = 156/290 (53%), Gaps = 6/290 (2%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M K A++FPGQGSQ M L FS + +LGYDL ++ +G ++L +T Sbjct: 1 MSKFAMVFPGQGSQSLGMLADLAAQFPIVEATFSEASSVLGYDLWQLVQQGPAEELNKTW 60 Query: 61 FAQPAIFAYSYAIFQSIWK--GKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERA 118 QPA+ S AI++ IW+ G K PS MAGHSLGE +AL CAGV ++ A+ LV R Sbjct: 61 QTQPALLTASVAIWR-IWQHQGGKL-PSLMAGHSLGEYSALVCAGVLDFKQAVSLVELRG 118 Query: 119 SLMQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSK-ALV 177 LMQ+A EG G M AI GL + + C S + N+NS QVVI+G+K A+ Sbjct: 119 KLMQEAVPEGTGAMYAIIGLDNESIAKACEE-SAQGQVVSPVNFNSPGQVVIAGNKDAVE 177 Query: 178 TQAAENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPY 237 A A ++PL VS P H ++ + L D +PV++NVD K Sbjct: 178 RAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVALEKIDFQEPLFPVVNNVDVKAE 237 Query: 238 SDPEGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKR 287 P IR+ L Q+ + VRW E+++++ +GV +E+GP LT L KR Sbjct: 238 VSPVAIRSALVRQLYNPVRWTESVEFIAAEGVELLLEIGPGKVLTGLTKR 287 Score = 176 bits (445), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 86/304 (28%), Positives = 143/304 (47%), Gaps = 10/304 (3%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M KIA LFPGQGSQ+ MGK LYE A+ +F ++ L L ++I EG+ ++LT T Sbjct: 1 MSKIAFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTY 60 Query: 61 FAQPAIFAYSYAIFQSIWK---GKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMER 117 AQPA+ S A+ + +K + + +A + Sbjct: 61 NAQPALLTTSIAVLE-KFKESGITP--DFTAGHSLGEYSALVAAGALSFKDAVYTVRKRG 117 Query: 118 ASLMQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVV-ANYNSSNQVVISGSK-A 175 + + A + L + E +V AN N Q+VISG+ Sbjct: 118 EFMNEAVPAGEGAMAAILGMDAEALKQVTDKVTE--EGNLVQLANLNCPGQIVISGTAKG 175 Query: 176 LVTQAAENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAK 235 + + +IPL+VS PFH+ ++ + + + VL A D+ + PV+SNV A Sbjct: 176 VELASELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSAD 235 Query: 236 PYSDPEGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAF 295 ++ I+ +L Q+ S VR++E+I+ L+ +GV TFIE+GP L+ L+K+ ++ Sbjct: 236 VMTEKADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVKKVNRRLKTI 295 Query: 296 SYEE 299 + + Sbjct: 296 AVSD 299 >BS_pksC [I] COG0331 (acyl-carrier-protein) S-malonyltransferase Length = 288 Score = 169 bits (427), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 104/289 (35%), Positives = 165/289 (57%), Gaps = 17/289 (5%) Query: 4 IALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTEFAQ 63 I +FPGQGSQ MG L++ E +E+ + ++ILGY + + E L +T+F Q Sbjct: 2 ITYVFPGQGSQKQGMGSGLFD---EFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQ 58 Query: 64 PAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASLMQQ 123 PA++ + + + ++ P F+AGHSLGE AL A F +E L+LV +R LM Sbjct: 59 PALYVVNALSYLKKIRDEEVKPDFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSL 118 Query: 124 ATTEGQGGMAAINGLHPLLVEEECARLSNDE--DKIVVANYNSSNQVVISGSKALVTQAA 181 + GGMAA+ GL+ EE+ A+ + + +AN N+ Q+VISG K + +AA Sbjct: 119 ISN---GGMAAVMGLN----EEQVAKALKEYHLHDVDIANVNAPYQIVISGKKDEIEKAA 171 Query: 182 ---ENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYS 238 E + ++ + +PL VS FH+ Y+ + + F+ L A S + PV+SNV AKPY+ Sbjct: 172 SLFETMTEVTMV-LPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYT 230 Query: 239 DPEGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKR 287 E ++ L+ Q+ +V+W ++I YL+++G F E+GP + LT LI R Sbjct: 231 Y-EFMKQTLADQINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHR 278 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,256,242 Number of extensions: 1145405 Number of successful extensions: 3158 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 3059 Number of HSP's successfully gapped: 46 Length of query: 425 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 316 Effective length of database: 25,807,025 Effective search space: 8155019900 Effective search space used: 8155019900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)