BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0007.p (905 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1649 [Q] COG3321 Polyketide synthase modules and related prot... 172 3e-42 all2646 [Q] COG3321 Polyketide synthase modules and related prot... 135 4e-31 all2635 [Q] COG3321 Polyketide synthase modules and related prot... 134 8e-31 BS_pksK_2 [Q] COG3321 Polyketide synthase modules and related pr... 127 1e-28 BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related pr... 119 2e-26 >all1649 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1570 Score = 172 bits (435), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 156/602 (25%), Positives = 260/602 (43%), Gaps = 45/602 (7%) Query: 264 HRLGWERSPLHRREQANASMPLTLVFAGQDPLSHRLLAALQERDMPFISVVMGERFNKQD 323 +R+ W+ SPL + + L+F+ L L A + + I V GE + K Sbjct: 947 YRIEWQLSPL-TVDNKTSEAERWLIFSDNQGLGQELAAVVNDS---CILVSSGESYEKLS 1002 Query: 324 DHHYRISGSQQ-DFDRLCGEIQTRGVQRILHCFSWSHGPSLPGSDVEQEMNKGLFSLFYL 382 HY+I+ Q DF +L +I + + ++++ WS S+ ++E + L YL Sbjct: 1003 SSHYQINPHQAADFQQLLADI-SEPITKVVYL--WSLDSSI---NLENSQPQCYSHLLYL 1056 Query: 383 TKSLIAQNIREKIDLVLIADLAYQVTRNEPSLNPMHSAFYHLGKVVSSEYAHLRCRCIDL 442 ++L + + L + A VT + +N + + +G+V++ EY L ID Sbjct: 1057 VQALAKISSKIAPQLWIGTQQAQAVTPSCHQINVAQTPLWGMGQVIALEYPQLWGGLIDF 1116 Query: 443 -DPETEVTALLAELDNLPDHYVTAYRKGERYGQRMMELPLSNPLPQELILSDSGCYLIXX 501 + + TA++AE+ A+R G+RY R+M P+S PLP L G YLI Sbjct: 1117 GNQDVAATAIIAEMTAKTGEDRVAFRDGKRYVARLM--PISAPLPAPQPLISDGSYLITG 1174 Query: 502 XXXXXXXXXXXXXXXKKPVNLALVSRSGFPDEAEWEDILQKGEAQEVIRRIKILQQIQEN 561 + +L L SR G +++E E Q+ IR + + Sbjct: 1175 GLGALGLTLAEWLVQQGARHLVLTSRQGLLNQSE--------EKQQKIRAL------ENQ 1220 Query: 562 GSTYTFYRADISRRTELENVLQDVRSRFGSINGVVHAAGVAGDQFLFNREEQRFREVIQP 621 G+T AD+S ++ + ++ + G++HAAGV D + + + F +V QP Sbjct: 1221 GATVKVVAADVSDYQQMSQLFAQIQLNSPKLRGIIHAAGVLNDCSIAQMDSETFTKVFQP 1280 Query: 622 KIAGSIYLAELITE-PLEFFVCYSSVSSLEGTPGQGDYVAANGFLDSFVY-----SLPAN 675 K+ G+ L +L + L+FFVC+SS+S+L G+ GQ Y AAN FLD VY LP Sbjct: 1281 KVTGAWNLHQLTQDLSLDFFVCFSSMSALLGSRGQLHYAAANSFLDGLVYHRQSLGLP-- 1338 Query: 676 QNGITLNWAPWKEIGMAYD-YETFNQELIFNLLPTGVAMSAFDAALASGEKQVVIGTINK 734 G+++NW PW E GMA YE + + L A+ L S Q ++ IN Sbjct: 1339 --GLSINWGPWAEGGMATQGYEVGLKRMGIEPLEPTAALQVLGGLLGSASMQTMVAAINW 1396 Query: 735 ENAVKNFRSSFFYEGNNLLMNYVEPQLVAQTSLSATASAGLPEGRTHNRSLQSIKELIRQ 794 F +G + + Q S L H R ++ ++ Sbjct: 1397 ----SAFGKIVAAKGRVAFLEALLTQESKDGSNGENFRQKLSAAPLHRRPALLTTQVQQE 1452 Query: 795 LWVTLLESESIA--LDDAFTSIGGNSIFAVYLLQELEKAFPGMLGISDIFTYPTINKMAE 852 + L S S ++ F +G +S+ +V LE L + +F P+I + Sbjct: 1453 VAQVLGHSGSYVPEVEQGFFDMGMDSLMSVQFRHSLEALLAVSLPSTLVFECPSIGDVVS 1512 Query: 853 YV 854 Y+ Sbjct: 1513 YL 1514 >all2646 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1488 Score = 135 bits (339), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 150/613 (24%), Positives = 256/613 (41%), Gaps = 89/613 (14%) Query: 258 LPKGMFHRLGWERSPLHRREQANASMPLTLVFAGQDPLSHRLLAALQERDMPFISVVMGE 317 +P + WERS H + LVF + ++ LQ I V GE Sbjct: 891 IPSPRLYLPSWERSLPHPQVDIAEKRLCWLVFTDNYGIYQEIINTLQAVGHDVIIVSQGE 950 Query: 318 RFNKQDDHHYRIS-GSQQDFDRLCGEIQTRGV--QRILHCFSWSHGPSLPGSDVEQEMNK 374 ++++ + ++ + QDF L +++ R + IL+ + + D++Q+ Sbjct: 951 KYDQPAYRTFTVNPQNSQDFVELWSDLELRELIPHHILYLWDLT--------DLQQQP-- 1000 Query: 375 GLFSLFYLTKSLIAQNIREKIDLVLIADLAYQVTRNEPSLNPMHSAFYHLGKVVSSEYAH 434 SL YL +++ +Q+I +I L L+ + + NE ++ PM + + +GKV+S E Sbjct: 1001 --LSLLYLAQAISSQSIEREITLTLVTNDGQLLLGNE-NIQPMKATVWGMGKVISQEIPQ 1057 Query: 435 LRCRCIDLDPETEVTA-------LLAELDNLPDHYVTAYRKGERYGQRMMELPLSNPLPQ 487 L CRC+D+ T + L+ E N + V AYR R+ Q L L + Sbjct: 1058 LTCRCVDISLSTVASTNEQIAQQLITECLNNQNDSVVAYRGNYRWNQIYKPL-LMDDAQS 1116 Query: 488 ELILSDSGCYLIXXXXXXXXXXXXXXXXXKKPVNLALVSRSGFPDEAEWEDILQKGEAQE 547 ++ + + G Y+I K V L L+S D++ + Sbjct: 1117 KISVHEGGNYVILGDLTTGLGTVFAEFLMKNSVRLVLLS-----------DVITE----- 1160 Query: 548 VIRRIKILQQIQENGSTYTFYRADISRRTELENVLQDVRSRFGSINGVVHAAGVAGDQ-- 605 + Q + G Y DI+++ ELEN + ++FG INGV ++ ++ + Sbjct: 1161 -----TLKQSLDNVGIDYLSLNIDITKQAELENAIAQAETKFGQINGVFYSTPMSNEHSM 1215 Query: 606 -FLFNREEQRFREVIQPKIAGSIYLAELITE-PLEFFVCYSSVSSLEGTPGQGDYVAANG 663 FL + + + KI G LAE++ + PL+F V SS+S++ G G Y AAN Sbjct: 1216 AFLPQLTTKHWEYNYRTKIQGLCVLAEVLRDKPLDFCVLQSSLSTVLGGLGLAVYAAANA 1275 Query: 664 FLDSFVYSLPANQNG----ITLNWAPWKEIGMAYDYET-FNQELIFNLL-----PTGVAM 713 +D F ++ NQN I++NW + D ET N NL+ P V Sbjct: 1276 VIDMF--AIQQNQNSKFPWISINWDACRS-----DLETDINHSFAANLVTLALTPAEVWQ 1328 Query: 714 SAFDAALASGEKQVVI--GTINK--ENAVKNFRSSFFYEGNNLLMNYVEPQLVAQTSLSA 769 S + VV+ ++K E V N + E NN +Y PQL + Sbjct: 1329 STQHIIKLGLPESVVVTKSDLDKRLEKWVTNTPTKTKTETNN---SYTRPQLTNE----- 1380 Query: 770 TASAGLPEGRTHNRSLQSIKELIRQLWVTLLESESIALDDAFTSIGGNSIFAVYLLQELE 829 + I+ I Q+W LL I ++D+F +GG+S+ A+ + L Sbjct: 1381 -----------YIAPRDEIETAIAQIWQDLLGISPIGINDSFFDLGGHSLLAIQAISRLR 1429 Query: 830 KAFPGMLGISDIF 842 F L + ++ Sbjct: 1430 DRFGVELSMRNLL 1442 >all2635 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1565 Score = 134 bits (336), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 158/627 (25%), Positives = 267/627 (42%), Gaps = 84/627 (13%) Query: 263 FHRLGWERSPLHRREQANASMPLT----LVFAGQDPLSHRLLAALQERDMPFISVVMGER 318 F+ W + PL R ++ PL LVF RL+ LQE ++V GE Sbjct: 953 FYLPSWRQKPLLR-----SAAPLNKQRYLVFCHAGNAGERLVQHLQETGQEVVTVKPGEG 1007 Query: 319 FNKQDDHHYRISGSQQDFDRLCGEIQTRGVQRILHCFSWSHGPSLPGSDVEQEMNKGLFS 378 F ++ D++ + +D+ L ++QT + SW+ ++ +Q+ N G +S Sbjct: 1008 FTQEGDNYAIAPHNPEDYKTLWLQLQTDDKLPDVIIHSWTLQER---TNFQQQQNLGFYS 1064 Query: 379 LFYLTKSLIAQNIREKIDLVLIADLAYQVTRNEPSLNPMHSAFYHLGKVVSSEYAHLRCR 438 L +LT++L + ++ + ++ V NE +LNP + L KV+ EY +L C+ Sbjct: 1065 LLWLTQALSSSSL----SIYVLTQQVQNVLGNE-NLNPDDATILGLVKVIPQEYPNLTCQ 1119 Query: 439 CIDLD-PETEVTALLAEL-------DNLPD-----HYVTAYRKGERYGQRMMELPLSNPL 485 ID+ + L+ E+ D LP AYR R+ Q P+S P Sbjct: 1120 HIDVSISDINFQQLVREINIPPVLSDTLPLEKGGLRMSVAYRGKTRWIQEFQ--PISLPE 1177 Query: 486 PQELILSDSGCYLIXXXXXXXXXXXXXXXXXKKPVNLALV--SRSGFPDEAEWEDILQKG 543 P +L L G Y+I VN LV RS P EW++ L Sbjct: 1178 PTQLPLITGGVYVIAGDLVEGLGLIYAKFLAAT-VNAKLVFLGRSDLPQPKEWDNWLATH 1236 Query: 544 EAQEVIRR-IKILQQIQENGSTYTFYRADISRRTELENVLQDVRSRFGSINGVVHAAGVA 602 Q+ + I+ LQ +Q G + F D++ +++E+ + +RFG INGV+H AGV Sbjct: 1237 GRQDAVSHCIQQLQGLQAQGCEFLFTTVDLAEISQVEDAIAQALTRFGKINGVIH-AGVM 1295 Query: 603 GDQ---FLFNREEQRFREVIQPKIAGSIYL-AELITEPLEFFVCYSSVSSLEGTPGQGDY 658 GD+ + N + KI G + L L +PL+FF+ SS+S++ G G Y Sbjct: 1296 GDRASCLIRNLTIPECEKQFHTKIHGLLALETALKNQPLDFFLLQSSLSAVVGGIGFAAY 1355 Query: 659 VAANGFLDSFVYSLPANQNG--ITLNWAPWKEIGMAYDYETFNQELIFNLLPTGVAMSAF 716 AN F+D+ + N N I++N W + +A + T + Sbjct: 1356 AGANCFMDAIAHQRTQNSNTHWISIN---WDAVSLAE-------------ITTSTGATLI 1399 Query: 717 DAALASGE-----KQVVIGTINKENAVKNFRSSFFYEGNNL---LMNYVEPQLVAQTSLS 768 D A+ S E ++++ I + V + + L N+ PQ+ A Sbjct: 1400 DLAMTSQEVWQVTERILAQPIAAQITVSPVDLASRQQSQPLEQSTTNHARPQITA----- 1454 Query: 769 ATASAGLPEGRTHNRSLQSIKELIRQLWVTLLESESIALDDAFTSIGGNSIFAVYLLQEL 828 T+ I+ + Q+ +LL I ++D F +GG+S+ A+ + +L Sbjct: 1455 -----------TYLPPSNEIEARVTQVMESLLGIAPIGVNDNFFELGGHSLLAIQAVSQL 1503 Query: 829 EKAFPGMLGISD-IFTYPTINKMAEYV 854 + F L + +F PTI +A+ + Sbjct: 1504 REEFQVELPMRQFLFESPTIGGIAKII 1530 >BS_pksK_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1575 Score = 127 bits (318), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 113/453 (24%), Positives = 204/453 (45%), Gaps = 66/453 (14%) Query: 424 LGKVVSSEYAHLRCRCIDLDPETEVTALLAELDN---LPDHYVTAYRKGERY--GQRMME 478 L K E++ L + I+++ E+ L +L + P Y G R+ G R M Sbjct: 1015 LFKTAEIEFSKLTAQVIEIEKPEEMIDLHLKLKDDSRRPFDKQIRYEAGYRFVKGWREMV 1074 Query: 479 LPLSNPLPQELILSDSGCYLIXXXXXXXXXXXXXXXXXKKP-VNLALVSRSGFPDEAEWE 537 LP ++ L + D G YLI + + L RS ++ E E Sbjct: 1075 LPSADTL--HMPWRDEGVYLITGGAGSLGLLFAKEIANRTGRSTIVLTGRSVLSEDKENE 1132 Query: 538 DILQKGEAQEVIRRIKILQQIQENGSTYTFYRADISRRTELENVLQDVRSRFGSINGVVH 597 L+ ++ G+ + AD+S + + ++L++++ R+G++NG++H Sbjct: 1133 -----------------LEALRSIGAEVVYREADVSDQHAVRHLLEEIKERYGTLNGIIH 1175 Query: 598 AAGVAGDQFLFNREEQRFREVIQPKIAGSIYLAELITE-PLEFFVCYSSVSSLEGTPGQG 656 AG + D+F+ ++ + F+EV+QPK++G +++ E + PL+FF+ +SSVS G GQ Sbjct: 1176 GAGSSKDRFIIHKTNEEFQEVLQPKVSGLLHVDECSKDFPLDFFIFFSSVSGCLGNAGQA 1235 Query: 657 DYVAANGFLDSFV---YSLPANQ----NGITLNWAPWKEIGM---AYDYETFNQELIFNL 706 DY AAN F+D+F SL A++ + I+ NW W+E GM A D + + Sbjct: 1236 DYAAANSFMDAFAEYRRSLAASKKRFGSTISFNWPLWEEGGMQVGAEDEKRMLKTTGMVP 1295 Query: 707 LPTGVAMSAFDAALASGEKQVVIGTINKENAVKNFRSSFFYEGNNLLMNYVEPQLVAQTS 766 +PT + AF + S + QV F EG + Q + Q Sbjct: 1296 MPTDSGLKAFYQGIVSDKPQV-----------------FVMEG--------QLQKMKQKL 1330 Query: 767 LSATASAGLPEGRTHNRSLQSIKE----LIRQLWVTL-LESESIALDDAFTSIGGNSIFA 821 LSA + A + R ++ ++ LI+ + L + ++ I ++ + G +S+ Sbjct: 1331 LSAGSKAKRNDQRKADQDQGQTRKLEAALIQMVGAILKVNTDDIDVNTELSEYGFDSVTF 1390 Query: 822 VYLLQELEKAFPGMLGISDIFTYPTINKMAEYV 854 ++ + F L + F Y ++ +AEYV Sbjct: 1391 TVFTNKINEKFQLELTPTIFFEYGSVQSLAEYV 1423 Score = 51.6 bits (122), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 58/274 (21%), Positives = 114/274 (41%), Gaps = 31/274 (11%) Query: 3 EHKIMDHYVLPGTAYLELAAQILKQT----VPEQGELIMENFMFLQPLQMKEHEQKSVHI 58 +H + VLPG AYLE+A + Q + + + + + +++QP+ + H + V I Sbjct: 641 DHVVRGKPVLPGVAYLEMAYAAINQAAGSEIGQDVRIRLNHTVWVQPVVVDRHSAQ-VDI 699 Query: 59 VLTKQKSGT---DVMVTSLSGELPDEWVVHCKGKVSILHREEPKRRIALPEVLQESKRQS 115 L ++ G D+ T G+ P V+H +G + E + ++ + S+R Sbjct: 700 SLFPEEDGKITFDIYSTQEDGDDP---VIHSQGSAELASAAE----TPVADLTEMSRRCG 752 Query: 116 VAGYKP------GAEAPVQTGPHWECMKEIYVEPDRIXXXXXXXXXXXXXXXXXTMHPSL 169 P G + GP ++ +K + + + +HPS+ Sbjct: 753 KGKMSPDQFYEEGRSRGMFHGPAFQGIKNVNIGNREVLAQLQLPEIVSGTNEQFVLHPSI 812 Query: 170 LDCAVNIP----ISQIRD-GLFLPFAYKRIKFYGSMPHRF--YSMLQMKNNQTTELQETV 222 +D A+ + ++ D L LPFA + ++ Y+ L ++ +Q+ Sbjct: 813 MDSALQTATICIMQELTDQKLILPFALEELEVIKGCSSSMWAYARLSDSDHSGGVVQKA- 871 Query: 223 SFDIVLFDEAGEVFAEIEEYSLKRVHIEAFSSAP 256 DI + DE+G V I+ +S + + E +S P Sbjct: 872 --DIDVIDESGTVCVRIKGFSTRVLEGEVHTSKP 903 >BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1206 Score = 119 bits (299), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 151/645 (23%), Positives = 245/645 (37%), Gaps = 101/645 (15%) Query: 257 VLPKGMFHRLGWERSPLHRREQANASMPLTLVFAGQDPLSHRLLAALQERDMPFISVVMG 316 ++PK F R W+ P+ E N ++ + L++ AL E + S Sbjct: 605 LMPKRCFLRKQWQPCPIEPAEMTNQTVAI---------LANEETMALAEELSAYFST--- 652 Query: 317 ERFNKQDDHHYRISGSQQ-------DFDRLCGEIQTRGVQRILHCFSWSHGPSLPGSDVE 369 YRI SQ+ D++ + G I L G Sbjct: 653 ----------YRIFDSQELDRVSAADYEHVAGAI------------------DLIGCGTS 684 Query: 370 QEMNKGLFSLFYLTKSLIAQNIREKIDL-VLIADLAYQVTRNEPSL--NPMHSAFYHLGK 426 E + G + + LI Q K L VL + NE L + Y + Sbjct: 685 HEHSMGWINWL---QKLIEQGRASKHHLTVLGVTKGLEAYANEGVLLSGASRAGLY---R 738 Query: 427 VVSSEYAHLRCRCIDLDPET---EVTALLA-ELDNLPDHYVTAYRKGERYGQRMMELPLS 482 ++ SEY+HL R D++ E E+ L+A E YR G+RY + E P Sbjct: 739 MLQSEYSHLTSRHADMECEASHEELARLIAVEYYAKSTESEVCYRNGQRYRAYLTEQPAE 798 Query: 483 NPLPQELI-LSDSGCYLIXXXXXXXXXXXXXXXXXKKPVN-LALVSRSGFPDEAEWEDIL 540 L + + S LI V L L+ R P +W + Sbjct: 799 AALSHKQVSFSTDKVLLITGGTRGLGLLCARHFVKTYGVKRLVLIGREELPPRDQWNSV- 857 Query: 541 QKGEAQEVIRRIKILQQIQENGSTYTFYRADISRRTELENVLQDVRSRFGSINGVVHAAG 600 + + +IK +Q++++ G+ D++ R +E L+ + G+I GV+H AG Sbjct: 858 ---KISSLAEKIKAVQELEDMGAQVQVLSLDLTDRVAVEQSLKTIHETMGAIGGVIHCAG 914 Query: 601 VAGDQ--FLFNREEQRFREVIQPKIAGSIYLAELI-TEPLEFFVCYSSVSSL--EGTPGQ 655 + Q + + +V++PK+ G L +L+ EPL FF +SSVS+ GQ Sbjct: 915 MVNKQNPAFIRKSLEEIGQVLEPKVEGLQTLFDLLQDEPLAFFTLFSSVSAAIPALAAGQ 974 Query: 656 GDYVAANGFLDSFVYSLPANQNGITLNWAPWKEIGMAYDYETFNQELIFNLLPTGVAMSA 715 DY AN F+D F + +++ W WKE G+ ++ L + Sbjct: 975 ADYAMANAFMDYFAEAHQDKCPIVSIQWPNWKETGLGEVRSKALEQTGLISLTNDEGLQL 1034 Query: 716 FDAALASGEKQVVIGTINKENAVKNFRSSFFYEGNNLLMNYVEPQLVAQTSLSATASAGL 775 D L+ + VV+ + N K P + Q SL A + Sbjct: 1035 LDQILSDRQYAVVLPAVPDTNVWK-------------------PDKLMQPSLPVEALSH- 1074 Query: 776 PEGRTHNRSLQSIKELIRQLWVTL------LESESIALDDAFTSIGGNSIFAVYLLQELE 829 PE + + E + L VTL + +E D+ F G +SI L+Q++ Sbjct: 1075 PETKEQTSTRNLFPETVDWL-VTLFSDELKIAAEDFETDEPFQEYGIDSIILAQLVQQMN 1133 Query: 830 KAFPGMLGISDIFTYPTINKMAEYVYSRMEQA---QPSSSAKANP 871 + G + S +F YPTI A ++ S+ + + QPS K P Sbjct: 1134 QQLNGDIDPSILFEYPTIESFAHWLISKYDISRVLQPSVPEKQTP 1178 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 64,118,763 Number of extensions: 2614709 Number of successful extensions: 15150 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 15150 Number of HSP's successfully gapped: 27 Length of query: 905 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 790 Effective length of database: 25,115,021 Effective search space: 19840866590 Effective search space used: 19840866590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)