BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0005.p (541 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related pr... 322 1e-87 BS_pksL_1 [Q] COG3321 Polyketide synthase modules and related pr... 315 9e-86 BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related pr... 298 1e-80 BS_pksK_3 [Q] COG3321 Polyketide synthase modules and related pr... 297 3e-80 all2646 [Q] COG3321 Polyketide synthase modules and related prot... 296 5e-80 >BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 211 Score = 322 bits (824), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 0/482 (0%), Positives = 0/482 (0%), Gaps = 35/482 (7%) Query: 12 LALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMAIAGGVC 71 + V + R C + Sbjct: 148 TQFAPGKNVE-QVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYK 206 Query: 72 LIFFPMKNPAFG---LVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRDPIHA 128 Sbjct: 207 - 265 Query: 129 VIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGDPIEV 188 Sbjct: 266 325 Query: 189 QSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHFDDPN 248 Sbjct: 326 385 Query: 249 PFIDFVGSPVYMVNKQQEWKR-------AQEDIPLRAGISSFGFSGTNLHLVLEE---AP 298 Sbjct: 386 445 Query: 299 VSPKLAPASRPYHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIE--LADLCYTAATGRGHY 356 Sbjct: 446 - 504 Query: 357 AYRLALIVRDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGL 416 Sbjct: 505 ---- 560 Query: 417 NLLHEQARAALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKIS 476 Sbjct: 561 ------------- 607 Query: 477 CW 478 Sbjct: 608 609 >BS_pksL_1 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1814 Score = 315 bits (808), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 183/477 (38%), Positives = 270/477 (56%), Gaps = 41/477 (8%) Query: 22 LSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMAIAGGVCLIFFPMKNPA 81 +++R+SYF + GPS+ +D+ CSS L A+H A ++IR+ ECD+A+AGGV L P K Sbjct: 477 IANRVSYFCNFHGPSMAVDTVCSSSLTAVHLALESIRHGECDVALAGGVNLSLHPNKYMT 536 Query: 82 FGLVE--SKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRDPIHAVIKGSAVNNDG 139 +G+ + S + F K DG + EGIG++LLKPL +A ED D I+AVIKGSAVN+ G Sbjct: 537 YGVWDMFSTDGHCRTFGKDGDGYVPAEGIGAVLLKPLRQAEEDGDRIYAVIKGSAVNHVG 596 Query: 140 RSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGDPIEVQSLSNAFRKYT 199 +G++ P+ ++Q DL I P TI + EAHGTGT LGDPIE+Q L AFR+YT Sbjct: 597 TVSGISVPSPVSQADLIETCLEKTGIDPRTISYVEAHGTGTSLGDPIEIQGLVKAFRQYT 656 Query: 200 SKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHFDDPNPFIDFVGSPVY 259 +QFC IGSVKSNIGH +A+GI+ + K L L HQKLV +LH ++ NP++DF SP Y Sbjct: 657 QDRQFCSIGSVKSNIGHAESAAGISGLSKVALQLHHQKLVPSLHSEELNPYVDFEKSPFY 716 Query: 260 MVNKQQEWKR-----AQEDIPL--RAGISSFGFSGTNLHLVLEE-----APVSPKLAPAS 307 + ++ + WK+ ED+P RAGISSFG +G+N H++LEE A L+ S Sbjct: 717 VQHETETWKQPVIKENGEDVPYPRRAGISSFGATGSNAHIILEEHIPQAAEQDVSLSSDS 776 Query: 308 RPYHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLALIVRDI 367 + +SA+ + L + R L + I++ DL YT GR R++ + I Sbjct: 777 DISAVIPLSARNQERLRVYAKRLLDFLHDG--IQIRDLAYTLQVGREPMEERVSFLASGI 834 Query: 368 QELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHEQARAAL 427 QEL ++L I+ H G S G E +R A Sbjct: 835 QELSDQLKAFIEGRKAIQHCWKGRV--------------SRGS----------EPSRPAE 870 Query: 428 GAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCW-PQADQ 483 + + + + +I + G+ +DW++ Y+ +RI LP YPF+++ W P+A++ Sbjct: 871 SVHKLLEQRKLDQIAEQWANGSGVDWKLLYEGSKPKRISLPTYPFERVRYWVPKAEK 927 >BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1206 Score = 298 bits (764), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 190/509 (37%), Positives = 275/509 (54%), Gaps = 26/509 (5%) Query: 2 YTHLLDQTDELALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNE 61 Y +L++ TG+ I ++RI YFL+LKGP+I +D+ACSS L+ H A QA+ N E Sbjct: 124 YGVMLNRQSRANATGNSFAIAAARIPYFLNLKGPAIPIDTACSSSLVGTHLARQALINKE 183 Query: 62 CDMAIAGGVCLIFFP---MKNPAFGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDK 118 DMA+ GGV L P M G++ Q K FD A+G + GEG G+L+LK L Sbjct: 184 IDMALVGGVSLYLTPESYMSMCEAGMLSPDGQC-KAFDNGANGFVPGEGAGALVLKRLKD 242 Query: 119 AVEDRDPIHAVIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGT 178 A DRD I+ +I GS +N DG++NG+TAP+A +Q DL + I PE+I + E HGT Sbjct: 243 AEADRDHIYGIIIGSGINQDGKTNGITAPSAKSQMDLERDIYETYGIHPESISYVEMHGT 302 Query: 179 GTVLGDPIEVQSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKL 238 GT GDPIE+++LS F++ T KKQFC IGSVKSNIGH +AA+G+A V K +L + H+ L Sbjct: 303 GTKQGDPIELEALSTVFQEKTDKKQFCAIGSVKSNIGHTSAAAGVAGVQKVLLCMNHKTL 362 Query: 239 VSTLHFDDPNPFIDFVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAP 298 V TL+F PN +F SP+Y+ + + W+ A + P RA +SSFG+SGTN H+V+EE Sbjct: 363 VPTLNFTTPNEHFEFEHSPLYVNTELKPWETA-DGKPRRACVSSFGYSGTNAHIVIEEYQ 421 Query: 299 VSPKLAPASRPYH--IFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHY 356 + ++ + +F +SAK++ L + + DI+L D+ YT TGR Sbjct: 422 PEKRNDRLTKQHRSALFVLSAKKEKQLKAYAEAMKDFVTSNEDIDLEDMAYTLQTGREAM 481 Query: 357 AYRLALIVRDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGL 416 YR+A + + L + L + E P I+ K K T ++ L Sbjct: 482 DYRMAFLADSREMLIKALDDYLAEMP-----NASIFAAHVKTKKSEIKLFETDHDAKALL 536 Query: 417 NLLHEQARAALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKIS 476 E+ R + ++ L+V+G ++DW Y RRI LP YPF + Sbjct: 537 QTWIEKKR-------------LEKVAELWVKGLQIDWNKLYGEYTPRRISLPAYPFAEEY 583 Query: 477 CW-PQADQPPGSRTPLSSPSEVFPGRKVL 504 W P + P + E+ P R L Sbjct: 584 YWLPTQEGEPETIATAMPQFELMPKRCFL 612 >BS_pksK_3 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1250 Score = 297 bits (761), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 180/479 (37%), Positives = 273/479 (56%), Gaps = 37/479 (7%) Query: 24 SRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMAIAGGVCLIFFPMKNPAF- 82 +R+SYFL++ GPS +++ACSS L+AIH A A++N +C+MAIAGGV I + ++ Sbjct: 156 NRMSYFLNIHGPSEPVETACSSSLVAIHRAVTAMQNGDCEMAIAGGVNTILTEEAHISYS 215 Query: 83 --GLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRDPIHAVIKGSAVNNDGR 140 G++ + + K F A+G + GEG+G ++LK L+ A D + I+ VI+G+A N+ GR Sbjct: 216 KAGMLSTDGRC-KTFSADANGYVRGEGVGMVMLKKLEDAERDGNHIYGVIRGTAENHGGR 274 Query: 141 SNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGDPIEVQSLSNAFRKYTS 200 +N LT+PN AQ DL +A+ A+I P T+ + EAHGTGT LGDPIE+ L AF++ ++ Sbjct: 275 ANTLTSPNPKAQADLLVRAYRQADIDPSTVTYIEAHGTGTELGDPIEINGLKAAFKELSN 334 Query: 201 KK---------QFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHFDDPNPFI 251 + CGIGSVKSNIGHL A+GI+ ++K +L +KH+ LV +LH + NP++ Sbjct: 335 MRGESQPDVPDHRCGIGSVKSNIGHLELAAGISGLIKVLLQMKHKTLVKSLHCETLNPYL 394 Query: 252 DFVGSPVYMVNKQQEWK----RAQEDIPLRAGISSFGFSGTNLHLVLEE-APVSPKLAPA 306 SP Y+V ++QEWK R ++P RAGISSFG G N H+V+EE P + A Sbjct: 395 QLTDSPFYIVQEKQEWKSVTDRDGNELPRRAGISSFGIGGVNAHIVIEEYMPKANSEHTA 454 Query: 307 SRPYHIFTVSAKEKTVLAEWVSRYLQHM--QRAGDIELADLCYTAATGRGHYAYRLALIV 364 + ++ +SAK K+ L + S+ L+ + ++ D +L + YT GR RLA + Sbjct: 455 TEQPNVIVLSAKNKSRLIDRASQLLEVIRNKKYTDQDLHRIAYTLQVGREEMDERLACVA 514 Query: 365 RDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHEQAR 424 +QEL+EKL + T D + G +S + K T I + E Sbjct: 515 GTMQELEEKLQAFVDGKEET----DEFFRG------QSHRNKETQTIFTA-----DEDMA 559 Query: 425 AALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCWPQADQ 483 AL A+ + + +++ L+V+G + W Y R I LP YPF K W A + Sbjct: 560 LALDAWIRK--RKYAKLADLWVKGVSIQWNTLYGETKPRLISLPSYPFAKDHYWVPAKE 616 >all2646 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1488 Score = 296 bits (759), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 158/358 (44%), Positives = 222/358 (62%), Gaps = 5/358 (1%) Query: 22 LSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMAIAGGVCLIFFPMKNPA 81 L++R+SY L+L+GPS+ + +ACS+ L+A+H ACQ++ + ECD+A+AGGV L Sbjct: 157 LTTRVSYKLNLRGPSLDIQTACSTSLVAVHVACQSLLSGECDIALAGGVSLAKATGYLYQ 216 Query: 82 FGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRDPIHAVIKGSAVNNDG-R 140 G + S + FD A GT+ G G+G ++LK L +A+EDRD IHAVIKGSA+NNDG Sbjct: 217 EGGIYSPDGHCRAFDADAQGTIAGSGLGIVVLKRLQEAIEDRDYIHAVIKGSAINNDGGL 276 Query: 141 SNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGDPIEVQSLSNAFRKYTS 200 TAP Q + A A + P I + E HGTGT LGDPIE+ +L+ AFR T Sbjct: 277 KVSYTAPRIDTQAAVIRAAQALAGVEPADISYIETHGTGTALGDPIEIAALTQAFRAGTE 336 Query: 201 KKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHFDDPNPFIDFVGSPVYM 260 K FC I SVK+NIGHL AA+GIAS++KT+L+LKHQ++ LHF PNP IDF +P Y+ Sbjct: 337 KTGFCAISSVKTNIGHLDAAAGIASLIKTVLALKHQQIPPNLHFQQPNPQIDFSQTPFYV 396 Query: 261 VNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAPVSPKLAPASRP--YHIFTVSAK 318 +W+ + P RAG+SSFG GTN H++LEEAP+ K+ + Y + +SAK Sbjct: 397 NTNLVDWET--DKTPRRAGVSSFGIGGTNAHVILEEAPIKFKIQNSKFKINYQMLCLSAK 454 Query: 319 EKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLALIVRDIQELQEKLHR 376 + L E ++Q+ LAD+ YT GR H+AYR ++ + +E + L + Sbjct: 455 SEKALEEATINLATYLQQHPQFNLADIAYTLHIGRQHFAYRRMVLCQTTEEAAQILEK 512 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 41,493,816 Number of extensions: 1765484 Number of successful extensions: 4901 Number of sequences better than 1.0e-05: 109 Number of HSP's gapped: 4573 Number of HSP's successfully gapped: 109 Length of query: 541 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 430 Effective length of database: 25,576,357 Effective search space: 10997833510 Effective search space used: 10997833510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)