BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0001.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1649 [Q] COG3321 Polyketide synthase modules and related prot... 155 6e-38 RSp0641_2 [Q] COG3321 Polyketide synthase modules and related pr... 147 2e-35 all1648_1 [Q] COG3321 Polyketide synthase modules and related pr... 135 8e-32 BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related pr... 134 2e-31 ML0139 [Q] COG3321 Polyketide synthase modules and related proteins 131 9e-31 >all1649 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1570 Score = 155 bits (392), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 86/200 (43%), Positives = 121/200 (60%), Gaps = 6/200 (3%) Query: 44 IAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVE-KDHAQYDK 102 IA+IG+ RF S QLL+ GVD+I E+PA R + + + K +A+Y Sbjct: 4 IAIIGIGCRFPGADSPQAFWQLLSNGVDAITEIPAERWNIDEFYDRNPETPGKMNARY-- 61 Query: 103 AGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGVFIG 162 G+LS++D FD FF ISPREA LMDP QRL L++AWEA+E+AG QLTGSKTGVF+G Sbjct: 62 GGFLSQVDRFDPHFFGISPREALLMDPQQRLLLEVAWEALEDAGIVREQLTGSKTGVFVG 121 Query: 163 YSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVVFDTACSSSLVA 222 S++ +Y + G + GN ++ A R+S+ +L+GP++ DTACSSSLVA Sbjct: 122 ISTN---DYSRIHPEFDSNPQGYDLTGNCINIAAGRLSYLFNLRGPSLAVDTACSSSLVA 178 Query: 223 IHFACRQLIEGSCDLXXXGG 242 +H AC+ + + G Sbjct: 179 VHLACQSIWNQESSMAIASG 198 >RSp0641_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1176 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 86/216 (39%), Positives = 121/216 (56%), Gaps = 10/216 (4%) Query: 41 DTDIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVEKDHAQY 100 D IA+IG+ RF L VD+I +P R+ P ++ +Q Sbjct: 1 DAPIAIIGLGCRFPGADDADAFWDALDGQVDAIGAVPQARRAAGTFDEPRAELP---SQV 57 Query: 101 DKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGVF 160 G+L +D FD AFF+ISP EA+ MDP QRL L++AW+A+E+AG + L GS TGVF Sbjct: 58 RLGGFLDRVDAFDAAFFSISPLEAARMDPQQRLALEVAWQALEDAGIAASGLAGSTTGVF 117 Query: 161 IGYSSDFGEEY--KKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVVFDTACSS 218 IG S+ E + G ++ V A GN S++A+R+S+ LDL+GP++ DTACSS Sbjct: 118 IGISTHDYENLQDRAGSELSVYSAT-----GNAGSIVANRLSYCLDLRGPSLAIDTACSS 172 Query: 219 SLVAIHFACRQLIEGSCDLXXXGGRDQAAAVPVKEP 254 SLVA+H AC+ L +G L GG + + EP Sbjct: 173 SLVAVHAACQSLRDGESTLALAGGVNLVLSTLSSEP 208 >all1648_1 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1041 Score = 135 bits (339), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 80/211 (37%), Positives = 118/211 (55%), Gaps = 15/211 (7%) Query: 38 KEADTDIAVIGVASRFSNTGSTSE-VEQLLTLGVDSIQELPAGRKE-----QADLFLPLF 91 + + IA++G+ RF + E +LL G D I +P R + D +P Sbjct: 28 RTQNEPIAIVGMGCRFPGDANNPEKFWELLRQGKDGITTVPPQRWDIDAYYDDDPDVP-- 85 Query: 92 QVEKDHAQYDKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQ 151 K +A+Y G+++ +D FD FF I+PREA +DP QRL L+++WEA+E AG + Sbjct: 86 --NKMYARY--GGFINNVDQFDPQFFGITPREAIALDPQQRLLLEVSWEALENAGIAPQK 141 Query: 152 LTGSKTGVFIGYSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVV 211 LTG++TGVF+G D + K+ I+ +P GN A R+S+ L L+GP++ Sbjct: 142 LTGTQTGVFVGIGID--DYAKRQIKHHIPIDAYTG-SGNAFCFAAGRLSYLLGLQGPSLA 198 Query: 212 FDTACSSSLVAIHFACRQLIEGSCDLXXXGG 242 DTACS+SLV IH AC+ L G C+L GG Sbjct: 199 IDTACSTSLVTIHLACQSLRNGECNLALAGG 229 >BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 211 Score = 134 bits (336), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 17/208 (8%), Positives = 39/208 (18%), Gaps = 14/208 (6%) Query: 43 DIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVEKDH--AQY 100 + + +R E + + + + + + Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD-VS---VNKATAKLYPVA 58 Query: 101 DK-AGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGV 159 + A L + S + + L + E L Sbjct: 59 NTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVG 118 Query: 160 F----IGYSSDFGEEYK-KGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVVFDT 214 + ++ FG + K+V + V Sbjct: 119 RKTANVVLNTAFGWPTIAVDTHIFR-VCNRTQFAPG-KNVEQVEEKLLKVVPAEFKVDCH 176 Query: 215 ACSSSLVAIHFACRQLIEGSCDLXXXGG 242 R+ GSC + Sbjct: 177 HWLILHGRYTCIARKPRCGSCIIEDLCE 204 >ML0139 [Q] COG3321 Polyketide synthase modules and related proteins Length = 447 Score = 131 bits (330), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 19/206 (9%), Positives = 44/206 (21%), Gaps = 13/206 (6%) Query: 42 TDIAVIGVASRF-SNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVEKDHAQY 100 I+ I + + E L + S P + + V + Sbjct: 6 KRISQISKETGVQNRVLVEKAQEIGLLVKSHSSSVGPGEERRLLEALNVKPVV-VEKMDS 64 Query: 101 DKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGVF 160 K E+ F + +A +R L A + Sbjct: 65 PKTLARVEVKENGDTFNVKAYEKAVKSYITKRKPLGEALDEEIMDELSVKFGIVDDALED 124 Query: 161 IGYSSDFGEEYKKGIQMLVPEAMGVAIP----GNIKSVIASRISHYLDLKGPAVVFDTAC 216 + ++ + + + G P + + ++ + D Sbjct: 125 LFKQI---QDAG----ISIVDKDGNPSPLALVTDEIEKEEVSDTAMDEIVTNVRIDDPVR 177 Query: 217 SSSLVAIHFACRQLIEGSCDLXXXGG 242 + L E + Sbjct: 178 MYLKEIGRYPLISLDEETKLAEAIIA 203 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,536,995 Number of extensions: 821842 Number of successful extensions: 1579 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 1537 Number of HSP's successfully gapped: 36 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)