BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0486.0010.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3448 [G] COG1879 ABC-type sugar transport system, periplasmic ... 121 2e-27 AGc5109 [G] COG1879 ABC-type sugar transport system, periplasmic... 119 4e-27 BH3840 [G] COG1879 ABC-type sugar transport system, periplasmic ... 104 2e-22 TM0114 [G] COG1879 ABC-type sugar transport system, periplasmic ... 103 5e-22 PM1325 [G] COG1879 ABC-type sugar transport system, periplasmic ... 94 2e-19 >BH3448 [G] COG1879 ABC-type sugar transport system, periplasmic component Length = 335 Score = 121 bits (303), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 73/278 (26%), Positives = 138/278 (49%), Gaps = 19/278 (6%) Query: 1 MIVKMNRG-DYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVII 59 M++ G DYW G E AA+ NV + ++ ++ DI EQIT++E++I + I Sbjct: 46 MMITFQSGMDYWKRCLKGFEDAAQALNVTVEYRGA-AQYDIQEQITVLEQAIAKNPAGIA 104 Query: 60 LAASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNG 119 ++A + L +++A IP+V D+ + +++G+N Y AG +A ++ +LL+G Sbjct: 105 ISAIDPVELTDTINKAVDAGIPIVLFDSGAPDSHAHSFLGTNNYNAGMNAAYKMAELLDG 164 Query: 120 YGEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFL-NYAARFPNVEVVQISYTSSNT 178 GE+ +I N + Q ER GF A FP +EV+ + ++ Sbjct: 165 EGEVAVITLPNQLNHQ---------------ERTTGFKETLEAEFPAIEVIAVEDGRGDS 209 Query: 179 ADAEQLTRDMLRNHPRLRGIATLNETASQGAGKVIQ-ELGPGPLKMVAFDSSPSMLEMLQ 237 + ++ +L ++P L GI G G ++ E G +++++FD+ L+++ Sbjct: 210 LHSRRVAHQLLEDYPNLAGIFATEANGGVGVGDAVRLESRAGEIQIISFDTDKGTLDLVD 269 Query: 238 DGTVQATVIQNPFNNGYLAVKYAVEATEGIDVPERVDT 275 +G + AT+ Q +N GY ++ Y G+ P+ + T Sbjct: 270 EGIISATLAQGTWNMGYWSLTYLFHLHHGLTEPQILQT 307 >AGc5109 [G] COG1879 ABC-type sugar transport system, periplasmic component Length = 154 Score = 119 bits (299), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 0/300 (0%), Positives = 0/300 (0%), Gaps = 19/300 (6%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVK-LTFKAPDSESDIDEQITMVEESIKQKADVII 59 I + V + + + + D++ ++ + I Sbjct: 72 AIAGLLELRGSGIVSVKSVPSGVLHFAVTTVLSPENPRLPEDDEALLMPCGGHLRLLRIP 131 Query: 60 LAASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAK---VKTYVGSNGYEAGQKSAERLIDL 116 Sbjct: 132 191 Query: 117 LNGYGEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSS 176 Sbjct: 192 -------------- 237 Query: 177 NTADAEQLTRDMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSMLEM 235 Sbjct: 238 297 Query: 236 LQDGTVQATVIQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNMLWPENQKLLF 295 Sbjct: 298 357 >BH3840 [G] COG1879 ABC-type sugar transport system, periplasmic component Length = 332 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 75/296 (25%), Positives = 146/296 (49%), Gaps = 20/296 (6%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIIL 60 +I + YW V+ GA A +F+V + +KAP S+ +EQ+ +++ +I K D II+ Sbjct: 50 LIAEEENNSYWRLVEKGAREAENQFDVLVEYKAP-KRSNPEEQLKLIDMAISAKVDGIIV 108 Query: 61 AASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGY 120 A + ++++A IPV+++D + + Y+G++ Y +G + + ++D + G Sbjct: 109 QAVNEEMFTPLINKAVDAGIPVITIDTDAPRSNRVAYIGTDNYYSGTIAGKAMLDDMEGD 168 Query: 121 GEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTAD 180 + +I + +S Q R +GF++ + P V +V++ ++ + + Sbjct: 169 IHVAVITGSLESSHQKL--------------RVQGFIDMISNEPRVTLVEVEASNISRLE 214 Query: 181 AEQLTRDMLRNHPRLRGIATLNETASQGAGKVI--QELGPGPLKMVAFDSSPSMLEMLQD 238 AE ++ HP + A +A G G V ++LG L ++AFD+ P L +L+ Sbjct: 215 AELKAHQLMEEHPHIN--AFYGTSALDGIGIVAAAEKLGLDDLYVIAFDTLPENLSLLEQ 272 Query: 239 GTVQATVIQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNM-LWPENQKL 293 + A V+Q PF G +V+ ++ EG T T +I ++ L E+Q+L Sbjct: 273 EKIDAIVVQEPFEMGLKSVELLLDIREGQPYSMTNHTHTSIIRKPDLSLLQEHQRL 328 >TM0114 [G] COG1879 ABC-type sugar transport system, periplasmic component Length = 335 Score = 103 bits (256), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 77/255 (30%), Positives = 126/255 (49%), Gaps = 29/255 (11%) Query: 10 YWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIILAASSYMGLA 69 YW+ V+ G +AA K V F P E DI+ Q+ M+E I + + I +A S + Sbjct: 44 YWSQVEQGVKAAGKALGVDTKFFVPQKE-DINAQLQMLESFIAEGVNGIAIAPSDPTAVI 102 Query: 70 QVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGYGEIGIINFT 129 + +A IPVV++D + + Y+G++ Y+AG + + +LL G G++ I Sbjct: 103 PTIKKALEMGIPVVTLDTDSPDSGRYVYIGTDNYQAGYTAGLIMKELLGGKGKVVI---- 158 Query: 130 NATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTADAEQLTRDML 189 GT A ++ +R +GF + A + +E+V I + A A L L Sbjct: 159 ----------GTGSLTAMNSLQRIQGFKD-AIKDSEIEIVDILNDEEDGARAVSLAEAAL 207 Query: 190 RNHPRLR---GIATLNETAS----QGAGKVIQELGPGPLKMVAFDSSPSMLEMLQDGTVQ 242 HP L G+ N A + AGKV G +K+V FD++P +L+ +++G +Q Sbjct: 208 NAHPDLDAFFGVYAYNGPAQALVVKNAGKV------GKVKIVCFDTTPDILQYVKEGVIQ 261 Query: 243 ATVIQNPFNNGYLAV 257 AT+ Q P+ GYL+V Sbjct: 262 ATMGQRPYMMGYLSV 276 >PM1325 [G] COG1879 ABC-type sugar transport system, periplasmic component Length = 314 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 67/278 (24%), Positives = 129/278 (46%), Gaps = 21/278 (7%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIIL 60 +++K +Y+ T++ GAE AKE N+ L + + E ++ + +VE I +K D II+ Sbjct: 34 LLMKTLSNEYFITMRQGAEEKAKEVNIDLIVQVAEKEDSTEQLVGLVENMIAKKVDGIIV 93 Query: 61 AASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVK------TYVGSNGYEAGQKSAERLI 114 + + +AE IP++ +D + K YVG + + G +A+ L Sbjct: 94 TPNDSIAFIPAFQKAEKAGIPIIDLDVRLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLA 153 Query: 115 DLLNGYGEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYT 174 LN G++ I+ G + ++R+ G L + PN++VV Sbjct: 154 QALNEMGDVAILEGIP--------------GVDNGEQRKAGALKAFSESPNIKVVASQSA 199 Query: 175 SSNTADAEQLTRDMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSML 233 + T A +T ++L HP ++GI N+ + GA I+ G G + + +D P + Sbjct: 200 NWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIENAGLAGKVLVSGYDGIPLAI 259 Query: 234 EMLQDGTVQATVIQNPFNNGYLAVKYAVEATEGIDVPE 271 E ++ G +Q T+ Q P +A++ ++ ++P+ Sbjct: 260 EYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPD 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,483,394 Number of extensions: 984111 Number of successful extensions: 1905 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 1885 Number of HSP's successfully gapped: 44 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)