BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0483.0005.p (240 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA2365 [IQR] COG1028 Dehydrogenases with different specificities... 75 6e-14 sll1704 [IQR] COG1028 Dehydrogenases with different specificitie... 69 5e-12 all0352 [IQR] COG1028 Dehydrogenases with different specificitie... 69 9e-12 all1420 [IQR] COG1028 Dehydrogenases with different specificitie... 67 2e-11 L118271 [IQR] COG1028 Dehydrogenases with different specificitie... 59 8e-09 >SA2365 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 234 Score = 75.5 bits (184), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 60/204 (29%), Positives = 90/204 (44%), Gaps = 16/204 (7%) Query: 1 MKKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIG 60 M K+ +TG ++GLG+ L YKV+ G + + QA + ++ V LD+ Sbjct: 1 MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRND----VRGQQASQKLGVHYVQLDVT 56 Query: 61 SSESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSLRV 120 S SVK A IA G LDI+INNAG+ Q + L D + +Y N G +R+ Sbjct: 57 SDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRD---VEEVYQTNVFGIVRM 113 Query: 121 SNAXXXXXXXXXXXXXVNISSEAGSI-------SRNQRINMYGYCMSKAALNMQSSLMHN 173 N VN+SS GS + ++N YC SK+A+ M + Sbjct: 114 MNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSSKSAVTMLTLQYAK 173 Query: 174 HLRVMGGQVMVFHPGWLQSYMSGE 197 L M Q+ PG + + G+ Sbjct: 174 GLPNM--QINAADPGATNTDLVGD 195 >sll1704 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 256 Score = 69.3 bits (168), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 62/251 (24%), Positives = 108/251 (43%), Gaps = 20/251 (7%) Query: 3 KVACVTGADRGLGFFLVRWLLEN---KYKVFAGQYFEESQALEALQAQYQDHLNLVPLDI 59 + + GA G+G VR+ LE K++A Y + L+ L + D L LVPLDI Sbjct: 13 QTVVIVGASGGIGLGFVRYFLEQVPFPVKIWA-TYRQAIAELDHLAKENPDRLTLVPLDI 71 Query: 60 GSSESVKRAARSIAGCTGHLDIIINNAGVIDQADDAT--ILVDMDDTAMAHIYNINTLGS 117 + + ++ + ++ +IN G++ D+ L + + + + +N++G Sbjct: 72 TEESQIAQFSQQLGKT--EVNWLINCVGILHVEDNPPEKSLRHLQPSQLQQYFAVNSIGP 129 Query: 118 LRVSNAXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRV 177 + ++ IS++ GSI NQ YGY SKAALNM R Sbjct: 130 VLLAKHFLPHLRHASPSVFATISAKVGSIGDNQLGGWYGYRASKAALNMLLKNTAIEYRR 189 Query: 178 MGGQ--VMVFHPGWLQSYMSGEKDEAAPIPPEE------SASKIMSIVEDHKAHMGKEPV 229 + Q V++ HPG + +S + +PPE+ + ++M+I+ K Sbjct: 190 VAPQCAVILLHPGTTDTSLSQPFQKN--VPPEKLFTVELTVKQLMAILLQVKPENSG--T 245 Query: 230 FLDFQGEPWPW 240 F + G PW Sbjct: 246 FYSWDGTILPW 256 >all0352 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 68.6 bits (166), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 58/216 (26%), Positives = 103/216 (47%), Gaps = 12/216 (5%) Query: 2 KKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHL--NLVPLDI 59 K++A VTG++RGLG+ + R L + V E+ L A Q + L + LD+ Sbjct: 5 KRIAVVTGSNRGLGYAISRKLAQIGIHVILTSR-NEADGLAAKQQLSSEGLDADYCVLDV 63 Query: 60 GSSESVKRAARSIAGCTGHLDIIINNAGV--IDQADDATILVDMDDTAMAHIYNINTLGS 117 + SV++ + + +DI++NNAG+ + +++++L +T M + N L Sbjct: 64 TNDVSVQKFTKWLHETYSKVDILVNNAGINPTTKPEESSLLTVQLET-MRFTWETNVLAV 122 Query: 118 LRVSNAXXXXXXXXXXXXXVNISSEAG---SISRNQRINMYGYCMSKAALNMQSSLMHNH 174 +R++ A VNIS+E SIS + Y +SK +N ++++ Sbjct: 123 VRITQALIPLMQAQNYGRIVNISTEMASLSSISDDYYPLAPSYRLSKVGVNGITAILAKE 182 Query: 175 LRVMGGQVMVFHPGWLQSYMSGEKDEAAPIPPEESA 210 L+ V + PGW+++ M G+ AP EE A Sbjct: 183 LQGTNILVNAYSPGWMKTDMGGDN---APFTAEEGA 215 >all1420 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 256 Score = 67.4 bits (163), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 62/248 (25%), Positives = 111/248 (44%), Gaps = 19/248 (7%) Query: 7 VTGADRGLGFFLVRWLLENKY--KVFAGQYFEESQALE--ALQAQYQDHLNLVPLDIGSS 62 + GA +G+G V+ LL++ K++A Y ++ A E L+ Y L + LDI Sbjct: 14 IVGASQGIGLGFVKKLLQDDRIAKIYA-TYRQKDSAFELITLENAYSQRLTCLSLDITDE 72 Query: 63 ESVKRAARSIAGCTGHLDIIINNAGVIDQAD--DATILVDMDDTAMAHIYNINTLGSLRV 120 + + I T L ++IN G++ + D L ++ + + IN++G++ + Sbjct: 73 LQIAEILQQINIETNRLHLVINCVGILHEGDFQPEKSLRQLNSENLLRYFQINSIGAVLL 132 Query: 121 SNAXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALN--MQSSLMHNHLRVM 178 + +IS++ GSI NQ YGY SKAALN M++ + Sbjct: 133 AKHLLPFFRHNEPSVFASISAKLGSIGDNQLGGWYGYRASKAALNMFMRTVAVEYGRSCP 192 Query: 179 GGQVMVFHPGWLQSYMSG--EKDEAAP--IPPEESASKIMSIVEDHKAHM--GKEPVFLD 232 V+ HPG + +S +K+ AA E + ++++++ AH+ G F Sbjct: 193 KTTVVTLHPGTTDTRLSRPFQKNVAAEKLFSIERTVDQLLAVI----AHLREGDSGKFFS 248 Query: 233 FQGEPWPW 240 + G PW Sbjct: 249 WDGSQLPW 256 >L118271 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 253 Score = 58.5 bits (140), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 50/195 (25%), Positives = 85/195 (43%), Gaps = 10/195 (5%) Query: 1 MKKVACVTGADRGLGFFLVRWLLENKYKVFAGQYFEESQALEALQAQYQDHLNLVPLDIG 60 M K+A VTGA +G+GF + + L + +KV Y EE+ A + ++ + D+ Sbjct: 1 MSKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAA--KELGENSFAIKADVS 58 Query: 61 SSESVKRAARSIAGCTGHLDIIINNAGVIDQADDATILVDMDDTAMAHIYNINTLGSL-R 119 E V A ++ G L++++NNAG+ TI + +YNIN G L Sbjct: 59 DREQVISALNAVVDKFGDLNVVVNNAGIAPTTQIETITPEQ----FHQVYNINVGGVLWG 114 Query: 120 VSNAXXXXXXXXXXXXXVNISSEAGSISRNQRINMYGYCMSKAALNMQSSLMHNHLRVMG 179 + +N +S+AG + N+ Y SK A+ + + L G Sbjct: 115 TQTSTALFRKLGHGGKIINATSQAGVVGNP---NLMLYSSSKFAVRGMTQIAARDLAEEG 171 Query: 180 GQVMVFHPGWLQSYM 194 V + PG +++ M Sbjct: 172 ITVNAYAPGIVKTPM 186 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,443,305 Number of extensions: 521536 Number of successful extensions: 2451 Number of sequences better than 1.0e-05: 58 Number of HSP's gapped: 2518 Number of HSP's successfully gapped: 58 Length of query: 240 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 137 Effective length of database: 26,499,029 Effective search space: 3630366973 Effective search space used: 3630366973 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)