BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0021.p (409 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1495 [E] COG0075 Serine-pyruvate aminotransferase/archaeal as... 233 4e-61 VC0392 [E] COG0075 Serine-pyruvate aminotransferase/archaeal asp... 232 9e-61 DR1350 [E] COG0075 Serine-pyruvate aminotransferase/archaeal asp... 189 6e-48 NMA1816_2 [QR] COG0500 SAM-dependent methyltransferases 165 1e-40 mlr6806 [E] COG0075 Serine-pyruvate aminotransferase/archaeal as... 138 2e-32 >SMa1495 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 370 Score = 233 bits (594), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 137/370 (37%), Positives = 182/370 (49%), Gaps = 19/370 (5%) Query: 9 LSPALRCIMTPGPVEVDPRVLRAMSFPVLGQFDPEFTDIMNETMGMLRELFATGNQWAFP 68 SP R +M PGP V P VL A + P +G DP F +M+ LR F T N+ FP Sbjct: 4 FSPPPRLLMGPGPSNVSPEVLAAQARPTIGHLDPSFVGLMDRIKDQLRLAFRTDNRVTFP 63 Query: 69 VDGTSRSGIEAVMVSLIAPGDRVLIPIFGRFGHLLHEIAERCGAEVFTIETAWGSVFAPE 128 + + +E +V+L+ PGD +I G FG + EIA+R GAEV + WG PE Sbjct: 64 LSAPASLAMEMALVTLLEPGDTAIIAQNGVFGGRMAEIAQRAGAEVRLVSVEWGKPVDPE 123 Query: 129 EVIAAI-RSFNPDVVAMVHGETSTGRVQPLAEIGRACRGHDALLIVDAVATIGGVPVETD 187 V A+I + ++A VH ETSTG A + R L +VD V +GG+PV D Sbjct: 124 AVRASILEAPQAKLLAFVHAETSTGVRSDAASLCALAREAGLLSVVDTVTGLGGIPVSVD 183 Query: 188 AWMLDAVIGGTQKCLSVPSGMAPVTYNERAEAKLMKRKQVERGLRTGGAARSELPVVGSN 247 W DAV GTQKCLS P G+AP+T+++RA + + RK + S Sbjct: 184 EWQADAVYAGTQKCLSAPPGLAPITFSDRAVSAVKARKT----------------PIQSW 227 Query: 248 YFDLGMLQDYWSEQ--RLNHHTEMTSMLYAXXXXXXXXXXXXXXKRYARHVLHERALTAG 305 + DLG++ YW + R HHT + LY +ARH L Sbjct: 228 FLDLGLMLGYWEGEGARSYHHTAPVNALYGLHESLSRLLGEGLETAWARHRAAHDRLVER 287 Query: 306 LAAMGLKLYGDPESKLTVVTCVLIPEGVDGESVRAMLLERFGIEIASSFGPLKGLIWRIG 365 L +G+ D E +L + V +PEGV R LL+ FGIEI GPL G IWRIG Sbjct: 288 LQGLGIAFVVDKEHRLPQLNTVWLPEGVKDVPERRRLLDEFGIEIGGGLGPLAGRIWRIG 347 Query: 366 TMGFSCRENN 375 MG +CR N Sbjct: 348 LMGETCRIEN 357 >VC0392 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 372 Score = 232 bits (591), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 132/379 (34%), Positives = 195/379 (51%), Gaps = 24/379 (6%) Query: 1 MTMKRYEDLSPALRCIMTPGPVEVDPRVLRAMSFPVLGQFDPEFTDIMNETMGMLRELFA 60 MT++ + P R +M PGP + P+VL+A+S P +G DP F +M+E +L+ F Sbjct: 1 MTIRSF---IPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQ 57 Query: 61 TGNQWAFPVDGTSRSGIEAVMVSLIAPGDRVLIPIFGRFGHLLHEIAERCGAEVFTIETA 120 T N++ V +G+EA V+L+ PGD+V++ G FG + + A RCGAEV ++ Sbjct: 58 TENEFTIAVSAPGSAGMEACFVNLVEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDE 117 Query: 121 WGSVFAPEEVIAAIRSFNPD--VVAMVHGETSTGRVQPLAEIGRACRGHDALLIVDAVAT 178 WG + E+V A I +PD ++A V ETSTG + +G + H L IVDAV + Sbjct: 118 WGCPVSVEKVQATIDE-HPDAKILAFVQAETSTGALSDAQALGALAKQHGLLTIVDAVTS 176 Query: 179 IGGVPVETDAWMLDAVIGGTQKCLSVPSGMAPVTYNERAEAKLMKRKQVERGLRTGGAAR 238 +GGVP+ D W LDAV G+QKCLS G+AP+T++ RA K+ RK Sbjct: 177 LGGVPLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKT------------ 224 Query: 239 SELPVVGSNYFDLGMLQDYWS--EQRLNHHTEMTSMLYAXXXXXXXXXXXXXXKRYARHV 296 + S + D ++ YWS +R HHT + LYA + RH Sbjct: 225 ----PIQSWFLDQSLVLGYWSGAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHR 280 Query: 297 LHERALTAGLAAMGLKLYGDPESKLTVVTCVLIPEGVDGESVRAMLLERFGIEIASSFGP 356 L L GL +G + +L + + +PEG++ VR+ LL + +EI + G Sbjct: 281 LMHEKLKQGLEQLGFQFVVAEPYRLPQLNAIYVPEGIEEAKVRSHLLNEYNLEIGAGLGA 340 Query: 357 LKGLIWRIGTMGFSCRENN 375 L G WRIG MG+ R N Sbjct: 341 LAGKAWRIGVMGYGARSEN 359 >DR1350 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 390 Score = 189 bits (481), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 126/360 (35%), Positives = 176/360 (48%), Gaps = 21/360 (5%) Query: 15 CIMTPGPVEVDPRVLRAMSFPVLGQFDPEFTDIMNETMGMLRELFATGNQ-WAFPVDGTS 73 ++TPGP + PR +A+ +LG DPE + +E LRE++ T + + + GT Sbjct: 11 VLLTPGPTPIHPRAQKALIRSMLGHMDPEVFALNSEIQRDLREMYGTEPEGFTALLAGTG 70 Query: 74 RSGIEAVMVSLIAPGDRVLIPIFGRFGHLLHEIAERCGAEVFTIETAWGSVFAPEEVIAA 133 G+EA +L+ GD VL+ G FG + E+A R GA+V + G P +V A Sbjct: 71 SLGMEAGFANLVEAGDEVLVCANGSFGRRMAEMAARYGAKVRLVTAPLGEAIKPADVAAH 130 Query: 134 IRSFNPDVVAMVHGETSTGRVQPLAEIGRACRGHDALLIVDAVATIGGVPVETDAWMLDA 193 + + +VA+VHGETSTG + P+ EI RG ALL VDAV T G P W +D Sbjct: 131 LDD-DISMVAVVHGETSTGVLNPVPEIAELVRGSGALLTVDAVTTAGMEPFHMQQWGVDY 189 Query: 194 VIGGTQKCLSVPSGMAPVTYNERAEAKLMKRKQVERGLRTGGAARSELPVVGSNYFDLGM 253 G QKCLS P G+APV +ERA ++ R R++ P+ Y D Sbjct: 190 AYTGAQKCLSAPPGLAPVAISERAFSRFAAR-------------RTQTPLW---YCDFEG 233 Query: 254 LQDYWSEQRLNHHTEMTSMLYAXXXXXXXXXXXXXXKRYARHVLHERALTAGLAAMGLKL 313 L+DYW HHT ++ +A R AR L R + LA +G Sbjct: 234 LRDYWVNHTY-HHTVPVNLHFAFHAALRAALEEGLEARQARVRLMGRVIERTLAPLGFSH 292 Query: 314 Y-GDPESKLTVVTCVLIPEGVDGESVRAMLLERFGIEIASSFGPLKGLIWRIGTMGFSCR 372 Y PE +L V + +PEG D +R L +R I + GP G+IWR+G MG S R Sbjct: 293 YVRRPEDRLPTVLALRLPEGFDDAGLRQALRQR-EISVTGGLGPTAGVIWRLGLMGESAR 351 >NMA1816_2 [QR] COG0500 SAM-dependent methyltransferases Length = 391 Score = 165 bits (417), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 127/410 (30%), Positives = 190/410 (46%), Gaps = 39/410 (9%) Query: 8 DLSPALRCIMTPGPVEVDPRVLRAMSFPVLGQFDPEFTDIMNETMGMLRELFATGNQWAF 67 DL L +M PGPV V PRVLRAMS P++ EF I + +L ++F T N F Sbjct: 10 DLEDTL--LMMPGPVPVTPRVLRAMSKPMINHRSAEFAGIYTDCRQILADVFQTKNDI-F 66 Query: 68 PVDGTSRSGIEAVMVSLIAPGDRVLIPIFGRFGHLLHEIAERCGAEVFTIETAWGSVFAP 127 + G+ +G+EA + S+ GD+V+ G+FG ++A A+V +E WG Sbjct: 67 LLSGSGTAGMEAAVGSVAGSGDKVIAIENGKFGQRFKDLAA-LYADVVPLEFEWGLPVDL 125 Query: 128 EEVIAAIRSFNPDVVAMVHGETSTGRVQPLAEIGRACRGHDALLIVDAVATIGGVPVETD 187 E V + + +VH ETS G + P EIG+ + HDA+ I+D V ++GG V+ D Sbjct: 126 EMVKEKLEE-GAKAITLVHNETSAGIMNPAVEIGKLAKKHDAIFIMDGVTSLGGDEVKVD 184 Query: 188 AWMLDAVIGGTQKCLSVPSGMAPVTYNERAEAKL--MKRKQVERGL----RTGGAARSEL 241 W +D I G+QKCL+ P GM+ V+ +E+A + MK++ L ++G R E Sbjct: 185 EWGVDIAIVGSQKCLAAPPGMSAVSVSEKAFEAINSMKKRPYYNDLKAYKKSGDKPRPET 244 Query: 242 PVVGSNYFDLGMLQDYWSEQRLNHHTEMTSMLYAXXXXXXXXXXXXXXKRYARHVLHERA 301 P +T + YA R RH A Sbjct: 245 P-----------------------YTPALPLFYAMQEALHIVKEEGMEVRIKRHRALSEA 281 Query: 302 LTAGLAAMGLKLYG--DPESKLT-VVTCVLIPEGVDGESVRAMLLERFGIEIASSFGPLK 358 + A AM ++++ + SK + VT + P GVDGE V+ + +R G+ IA LK Sbjct: 282 VRAAAGAMNIEMFPQLNEYSKYSNTVTAMKAPAGVDGEDVKNDMKKR-GVIIAGGQEHLK 340 Query: 359 GLIWRIGTMGFSCRENNXXXXXXXXXXXXXXHGYAVPCGLGVQAALDVYE 408 G I+RIG MG + + GY G G +AA+ V + Sbjct: 341 GKIFRIGNMG-NVTARDVLSTIQQLEIVLSKQGYIDSVGAGAEAAMRVID 389 >mlr6806 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 301 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 0/411 (0%), Positives = 0/411 (0%), Gaps = 24/411 (5%) Query: 3 MKRY-EDLSPALRCIMTPGPVEVDPRVLRAMSFPVLGQFDPEFTDIMNETMGMLRELFAT 61 M I+ V + + + + T + Sbjct: 1 MSIDKSYCGFIA--IVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQA 58 Query: 62 GNQWAFPVDGTSRSGIEAVMVSLIAPGDRVLIPIFGRFGHLLHEIAERCGAEVFTIETAW 121 +++ A + + + E Sbjct: 59 IYVDTP-GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKA 117 Query: 122 GSVFAPEEVIAAIRSFNPD--VVAMVHGETSTGRVQPLAEIGRACRGHDALLIVDAVATI 179 + A +V + + + + + P++ A ++ + Sbjct: 118 PVILAVNKVDNVQEK-ADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEA 176 Query: 180 GGVPVETDAWMLDAVIGGTQKCLSVPSGMAPVTYNERAEAKLMKRKQVERGLRTGGAARS 239 E ++ ++ + ERG Sbjct: 177 THHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERG--------G 228 Query: 240 ELPVVGSNYFDLGMLQDYWSEQRLNHHTEMTSMLYAXXXXXXXXXXXXXXKRYARHVLHE 299 + Q+ + + + + Sbjct: 229 YDINGL--------ILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVK 280 Query: 300 RALTAGLAAMGLKLYGDPESKLT-VVTCVLIPEGVDGESVRAMLLERFGIEIASSFGPLK 358 Sbjct: 281 340 Query: 359 GLIWRIGTMGFSCRENNXXXXXXXXXXXXXXHGYAVPCGLGVQAALDVYEA 409 Sbjct: 341 391 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,326,825 Number of extensions: 1166060 Number of successful extensions: 2360 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2326 Number of HSP's successfully gapped: 18 Length of query: 409 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 300 Effective length of database: 25,807,025 Effective search space: 7742107500 Effective search space used: 7742107500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)