BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0008.p (394 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr2975 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 203 5e-52 BMEII1134 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A... 197 2e-50 DR1377 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 117 3e-26 mll3370 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 106 7e-23 AGl3143 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 106 7e-23 >mlr2975 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 203 bits (516), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 0/402 (0%), Positives = 0/402 (0%), Gaps = 28/402 (6%) Query: 3 VHNLYGAFIE-PELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHS 61 + G V S ++ + Sbjct: 4 DKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDT 63 Query: 62 AVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXXX 121 + M + + + + + + Sbjct: 64 PGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAV 123 Query: 122 -XDFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQ 180 + + + + E + A Sbjct: 124 NKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPED 183 Query: 181 AGEVLLGAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIAP 240 R A + +++ + + G +I Sbjct: 184 YITDRSQ------RFMASEII----REKLMRFLGAELPYSVTVEIERFVSNERGGYDING 233 Query: 241 EGLKTWMN--------VFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQ-- 290 L +++ I A P+ Sbjct: 234 LILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALR 293 Query: 291 --DHSRALRMRGEIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCI 348 Sbjct: 294 SL---- 349 Query: 349 AGLAGLPQVTLPVIGPAGLPLGLSVIGGHGQDLRLLSWVREI 390 Sbjct: 350 391 >BMEII1134 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 401 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 137/409 (33%), Positives = 195/409 (47%), Gaps = 26/409 (6%) Query: 1 MAVHNLYGAFI-EPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAA 59 MA + + A I +PE + + SG LAG AVKD++ +AG + GNP L P + Sbjct: 1 MANLDPFNALIAKPEKPLAHAASGPLAGERLAVKDIYDIAGMVTGCGNPQILAESPVARK 60 Query: 60 HSAVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXX 119 + VV LL +GA G TDE+ +S+ G+N HY P+NP A DR+ Sbjct: 61 SAPVVEKLLAAGAEFIGKAQTDEVAFSMMGQNSHYPYPINPAAPDRVTGGSSSGSAAAVA 120 Query: 120 XX-XDFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELL 178 D ALG+DTGGS+R P+++CG+ G R THG + +EG++PLAP DT+GW L Sbjct: 121 GKLADIALGSDTGGSIRTPASFCGLIGLRSTHGRIPLEGIMPLAPSLDTIGWFARDIALY 180 Query: 179 LQAGEVLLGAAAPGSRGGAMGKLHIAP--------EAWXXXXXXXXXXXRNGLQLLQAGA 230 + G VLLG A + + +L P +A R L+A + Sbjct: 181 EKVGAVLLGDDAQEFK---LTQLLYMPVLEQLLLGQAETDAYRAMFAKVRPHFATLKAAS 237 Query: 231 GWSGATEIAPEGLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQ 290 + + + F ++QGAE W HGAWI GP I Sbjct: 238 QPTLS-------IDELYLAFSQIQGAEAWENHGAWISSGNRQLGPGIADRFAYGAEMTAD 290 Query: 291 DHSRALRMRGEIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAG 350 R A ++GE + + LPTVPG APL G L+ R A+ L C++G Sbjct: 291 MVKNQRARRARFAEEFEAIIGESAVLALPTVPGAAPLTGEPFEALQAYREQALRLLCLSG 350 Query: 351 LAGLPQVTLPV--IGPAGL----PLGLSVIGGHGQDLRLLSWVREIWQQ 393 L+GLPQ+TLP+ + P G P G+S IG G D L++ + I + Sbjct: 351 LSGLPQITLPLGSLRPGGTMYDAPFGISFIGPRGSDRTLIALAQTIISE 399 >DR1377 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 394 Score = 117 bits (293), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 108/383 (28%), Positives = 164/383 (42%), Gaps = 56/383 (14%) Query: 22 SGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLLQSGATLRGATHTD 81 +G LAG +F+VKD++ V G +A R+ P S +V LL+ GA G TH Sbjct: 32 AGPLAGLTFSVKDLYGVPGWPLTAST----RAPVPDPGASVLVGRLLELGAAALGKTHLH 87 Query: 82 ELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXX-XXDFALGTDTGGSVRVPSAY 140 E+ + G N + GT +P +R+P DFALGTDTGGS+RVP+A+ Sbjct: 88 EVALGITGMNGYGGT-THPRDPERVPGGSSSGAAVSVALGQVDFALGTDTGGSIRVPAAW 146 Query: 141 CGVYGFRPT--HGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLGAAAPGSRGGAM 198 C V G++PT H A EGV+PL+ D G + ++++ + A+ Sbjct: 147 CSVVGYKPTKGHPAWSTEGVLPLSWTCDHAGPLARDLSMVVRVYQ-------------AL 193 Query: 199 GKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGAT-----------------EIAPE 241 G H+ P+ W R GL L + GW+ T + P Sbjct: 194 GGGHVTPQDW--------QDVRVGLWLPE---GWTDETVRDAVLAYADDLKARGATVTPV 242 Query: 242 GLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRGE 301 L ++ + + +E H +++ P F P ++ A R E Sbjct: 243 DLPEMLDAYSPIVLSEAARVHAEALQQPDPGFLPFTLMSLRNGAALSEEEVQAAHERRTE 302 Query: 302 IAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQN----RSGAMMLSCIAGLAGLPQV 357 L +LL E ++ P VP P PL G D L + R + L+ L G P + Sbjct: 303 YRVLLDRLLAEFDVLLAPAVPTPPPLIGQDEVTLLEGPQVLRRAVLRLNTPFSLLGAPVL 362 Query: 358 TLPVIGPAGLPLGLSVIGGHGQD 380 +LP P +G+ ++G HGQD Sbjct: 363 SLPSSVPD---VGVQLVGRHGQD 382 >mll3370 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 106 bits (264), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 0/382 (0%), Positives = 0/382 (0%), Gaps = 17/382 (4%) Query: 23 GKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLLQSGATLRGATHTDE 82 + D + A N + S+ + + + G Sbjct: 51 HTEGAYQAIYVDTPGLHMEEKRAINR-LMNKAASSSIGDVELVIFVVEGTRWTPDDEMVL 109 Query: 83 LMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXX-XXDFALGTDTGGSVRVPSAYC 141 G +++ + +TG +V +A Sbjct: 110 NKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIV 169 Query: 142 GVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLGAAAPGSRGGAMGKL 201 + TH E + L + G Sbjct: 170 RKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGY 229 Query: 202 HI-APEA--WXXXXXXXXXXXRNGL-QLLQAGAGWSGAT---EIAPEGLKTWMNVFRELQ 254 I + + + E + + + + Sbjct: 230 DINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDE 289 Query: 255 GAEIWATHGAWIERAQPL-FGPDIXXXXXXXXXXXGQDHSRALRMRGEIAARLRQLLGED 313 A Sbjct: 290 RALR--- 346 Query: 314 SCIVLPTVPGPAP--LRGGDLAQLEQNRSGAMMLSC-IAGLAGLPQVTLPVIGPAGLPLG 370 Sbjct: 347 - 405 Query: 371 LSVIGGHGQDLRLLSWVREIWQ 392 Sbjct: 406 427 >AGl3143 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 144 Score = 106 bits (264), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 0/382 (0%), Positives = 0/382 (0%), Gaps = 21/382 (5%) Query: 19 GSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLLQSGATLRGAT 78 A V + S Sbjct: 14 PETLADAAKRLGGGPVVMINFLRFRDRPL-YSDGFEHVKTTSRSAYYEGYASAFQQIATA 72 Query: 79 HTDELMYSLGGENYHYGTPVNPLAKD--RIPXXXXXXXXXXXXX-XXDFALGTDTGGSVR 135 G P D I + Sbjct: 73 LNISTELIYAGSQLMTLLPKESEVWDDIVIVRYDSFADLCSIIDTPEYALQAEPHREASV 132 Query: 136 VPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLG-AAAPGSR 194 Sbjct: 133 192 Query: 195 GGAMGKLHIAPEAWXXXXX---XXXXXXRNGLQLLQAGAGWSGATEIAPEGLKTWMNVFR 251 Sbjct: 193 --- 249 Query: 252 E---LQGAEIWATHGAWIERAQPL-FGPDIXXXXXXXXXXXGQDHSRALRMRGEIAARLR 307 Sbjct: 250 309 Query: 308 QLLGEDSCIVLPTVP----GPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPV-I 362 Sbjct: 310 - 368 Query: 363 GPAGLPLGLSVIGGHGQDLRLL 384 Sbjct: 369 390 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,605,997 Number of extensions: 1249037 Number of successful extensions: 2192 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2131 Number of HSP's successfully gapped: 54 Length of query: 394 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 286 Effective length of database: 25,922,359 Effective search space: 7413794674 Effective search space used: 7413794674 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)