BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0065.p (226 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0753 [O] COG1651 Protein-disulfide isomerase 99 7e-21 AGc1464 [O] COG1651 Protein-disulfide isomerase 71 2e-12 BMEI1440 [O] COG1651 Protein-disulfide isomerase 65 8e-11 CC0375 [O] COG1651 Protein-disulfide isomerase 64 2e-10 mlr7525 [O] COG1651 Protein-disulfide isomerase 63 3e-10 >DR0753 [O] COG1651 Protein-disulfide isomerase Length = 262 Score = 98.6 bits (244), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 69/182 (37%), Positives = 93/182 (51%), Gaps = 10/182 (5%) Query: 49 YEKQPHLGSENAKVKVIEFADFKCPACKKWTEAYLDTFIKDYVDTGKVQLYFMNFAFL-D 107 Y P+ G NA V V+ DFKCP CK + E YV TGKV++Y + + FL D Sbjct: 81 YANLPYAGQANAPVNVLVVEDFKCPNCKSFEETVAPELRTKYVGTGKVKMYSLVYPFLAD 140 Query: 108 R----DSYLAASAGEAIYKQS-NEKFWEYLHKLYENQGDESKIWATRKFILNFVKKNIDG 162 R DS AA A +Y Q N+ F Y L+ QG E+++WAT K L + ++D Sbjct: 141 RLPEDDSKYAAQAARCVYAQGKNDAFNTYKEILFRAQGPETEVWAT-KSRLKELATSLD- 198 Query: 163 IDYAKFEQDLKNQTYMYDVKEDFKIAGSYGVNGTPKFMVDGVLLPDSS--YEGLTAAIEA 220 ID AKF L N V+ D + A GV GTP V+G L+ S + ++AAI+ Sbjct: 199 IDQAKFATCLDNDETAAQVETDKQEALKAGVGGTPTVFVNGKLVNVQSDYVKDISAAIDE 258 Query: 221 GL 222 L Sbjct: 259 AL 260 >AGc1464 [O] COG1651 Protein-disulfide isomerase Length = 301 Score = 70.9 bits (172), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 19/199 (9%), Positives = 42/199 (21%), Gaps = 16/199 (8%) Query: 27 AELDDLPNYT-DVKGTIVVDGLKYEKQPH--LGSENAKVKVIEFADFKCPACKKWTEAYL 83 + G + + + + K A + L Sbjct: 35 ITSRKAQTTRHRIVGIHT---EGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVEL 91 Query: 84 DTFIKDYVDTGKVQLYFMNFAFLDRDSYLAASAGEAIYKQSNE-KFWEYLHKLYENQGDE 142 F+ + +N + A A+ K N + + L L Sbjct: 92 VIFVVEGTRWTPDDEMVLNKLREGK-----APVILAVNKVDNVQEKADLLPHLQFLASQM 146 Query: 143 SKIWATRKFILNFVKKNIDGIDYAKFEQDLKNQTYMYDVKEDF-KIAGSYGVNGTPKFMV 201 + + + +D I + + + + K M Sbjct: 147 NFLDIVPISAETGLN--VDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMR 204 Query: 202 DG-VLLPDSSYEGLTAAIE 219 LP S + + Sbjct: 205 FLGAELPYSVTVEIERFVS 223 >BMEI1440 [O] COG1651 Protein-disulfide isomerase Length = 217 Score = 65.1 bits (157), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 52/191 (27%), Positives = 87/191 (45%), Gaps = 21/191 (10%) Query: 43 VVDGLKYEKQPHL-----GSENAKVKVIEFADFKCPACKKWTEAYLDTFIKDYVDTGKVQ 97 +VD ++ K L G +A V ++E+A CP C + + Y+DTGK + Sbjct: 37 IVDAIEIAKPGKLKDMVYGKADAPVTIVEYASLTCPHCADFKLITFPKIKEKYIDTGKAR 96 Query: 98 LYFMNFAFLDRDSYLAASAGEAIYKQSNEKFWEYLHKLYENQGDESKIWAT----RKFIL 153 L F +F F R A+A + + + E + + L+ Q + WAT + +L Sbjct: 97 LIFRDFPFDPR-----ATAAVMLARCAPEDHYFPMIDLFFRQQQQ---WATAEDGKAALL 148 Query: 154 NFVKKNIDGIDYAKFEQDLKNQTYMYDVKEDF-KIAGSYGVNGTPKFMVDG-VLLPDSSY 211 K + G FE L NQ + DV+ + + +GVN TP F ++G D S+ Sbjct: 149 QIAK--LAGFTQESFEACLTNQQLLNDVRATVERGSKEFGVNATPTFFINGKKYAGDLSF 206 Query: 212 EGLTAAIEAGL 222 E ++ I++ L Sbjct: 207 EEMSGFIDSAL 217 >CC0375 [O] COG1651 Protein-disulfide isomerase Length = 204 Score = 64.3 bits (155), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 49/177 (27%), Positives = 80/177 (45%), Gaps = 15/177 (8%) Query: 55 LGSENAKVKVIEFADFKCPACKKWTEAYLDTFIKDYVDTGKVQLYFMNFAFLDRDSYLA- 113 LG NAKV VIE+A CP C W F Y+DTGKV+ ++ RD+ Sbjct: 35 LGDPNAKVTVIEYASVACPHCATWNAEVFPAFKAKYIDTGKVK-------YVHRDALTGE 87 Query: 114 ---ASAGEAIYKQSN-EKFWEYLHKLYENQGDESKIWATRKFILNFVKKNIDGIDYAKFE 169 A+AG + + + +K+++ LY Q + R +L + G++ A+F Sbjct: 88 PRLANAGAMLARCAGKDKYFQVTEALYRAQTNIFTSGDIRGELLTIAQA--AGMNEAQFN 145 Query: 170 QDLKNQTYMYDVKEDFKIAGSYGVNGTPKFMVDGVLL-PDSSYEGLTAAIEAGLAKA 225 L ++ + K+ + GTP F V+G L + E A ++A +A+A Sbjct: 146 SCLSDENAAKSAERIEKMMTDNNIRGTPTFEVNGKRLGGEEGGEQTLAQLDAAIAEA 202 >mlr7525 [O] COG1651 Protein-disulfide isomerase Length = 301 Score = 63.2 bits (152), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 8/151 (5%), Positives = 19/151 (12%), Gaps = 8/151 (5%) Query: 54 HLGSENAKVKVIEFADFKCPACKKWTEAYLDTFIKDYVDTGKVQLYFMNFAFLDRDSYLA 113 V I VQ L Sbjct: 87 GDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQ-----F 141 Query: 114 ASAGEAIYKQSNEKFWEYLHKLYENQGDESKIWATRKFILNFVKKNIDGIDYAKFEQDLK 173 ++ L+ + D + ++ Sbjct: 142 LASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYI--TDRSQRFMASEIIR 199 Query: 174 NQTYMYDVKEDFKI-AGSYGVNGTPKFMVDG 203 + + E + + Sbjct: 200 EKLMRFLGAELPYSVTVEIERFVSNERGGYD 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,837,495 Number of extensions: 682476 Number of successful extensions: 1320 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1322 Number of HSP's successfully gapped: 10 Length of query: 226 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 123 Effective length of database: 26,499,029 Effective search space: 3259380567 Effective search space used: 3259380567 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)