BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0061.p (488 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YER087w [J] COG0442 Prolyl-tRNA synthetase 199 6e-51 SPBC24C6.03 [J] COG0442 Prolyl-tRNA synthetase 187 2e-47 BS_thrZ [J] COG0441 Threonyl-tRNA synthetase 60 7e-09 SPBC25H2.02 [J] COG0441 Threonyl-tRNA synthetase 60 1e-08 STM0740 [C] COG1271 Cytochrome bd-type quinol oxidase, subunit 1 56 1e-07 >YER087w [J] COG0442 Prolyl-tRNA synthetase Length = 346 Score = 199 bits (507), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 0/481 (0%), Positives = 0/481 (0%), Gaps = 54/481 (11%) Query: 5 RQSHLL-ATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 + E + + Y + + R + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSE-ADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 64 MDA-AGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMR 122 A+ + A R K + + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNN--DKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 123 GEISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYK 182 ++ G + Y + + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 183 AYQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEV 242 + + + + ++ + + + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLN-RSFEVGLAEVSQYEGV---ELLAYSC------ 235 Query: 243 HTEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLVR 302 + K+ + + + G+ V Sbjct: 236 ----------LGFGTLTGKYL----------NGAKPAGARNTLFSRFTRYSGEQTQKAVA 275 Query: 303 GDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMTS 362 ++ + ++ S Sbjct: 276 AYVDIARRHGLDPA--------QMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELS 327 Query: 363 GITGAGE-RDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFK 421 A Sbjct: 328 EDVLAEIE- 386 Query: 422 LGTKYSEKLGASVLDANGRSKLMI-MGCYGIGISRLVXXXXLQWLSRTTMGRGLSGLRHW 480 Sbjct: 387 -------- 438 Query: 481 P 481 Sbjct: 439 439 >SPBC24C6.03 [J] COG0442 Prolyl-tRNA synthetase Length = 346 Score = 187 bits (476), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 19/242 (7%), Positives = 38/242 (15%), Gaps = 3/242 (1%) Query: 1 LKNMRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIV 60 + L E + + A +A GI + Sbjct: 5 RIPHSSLEVSTLGLGTMTF-GEQNS-EADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 REEMDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSL 120 G S + + + + ++ DR+ + H+ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 121 MRGEISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRM 180 P K + + + L A G + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 181 YKAYQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQA 240 R F V Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSL-LNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 241 EV 242 Sbjct: 242 TG 243 Score = 58.9 bits (141), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 8/110 (7%), Positives = 15/110 (13%), Gaps = 11/110 (10%) Query: 357 VAAMTSGITGAGERDFHLR-----DVV---PGRDFALE-HVADFRTAEEGETCPVCGKGV 407 R F + L + + Sbjct: 200 RIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTL- 258 Query: 408 LHFQQGIEIGHIFKLGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLV 457 K Y + + L + S L+ Sbjct: 259 FSRFTRYSGEQTQKAVAAYVDIARRHG-LDPAQMALAFVRRQPFVASTLL 307 >BS_thrZ [J] COG0441 Threonyl-tRNA synthetase Length = 346 Score = 60.1 bits (144), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 0/129 (0%), Positives = 0/129 (0%), Gaps = 2/129 (1%) Query: 39 AAGIYTYLPLGRKVLRKIEAIVREEMDAAGAQELLMPSMQPAELWKESERYEVYGKELMK 98 A A A + A + + K ++ Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQL-KTNIE 320 Query: 99 LSDRHDREFVLGPTHEEVITSLMRGEISSYRKLPITVYQIGTKFRDERR-PRSGLMRGRE 157 Sbjct: 321 380 Query: 158 FLMKDAYSF 166 Sbjct: 381 389 >SPBC25H2.02 [J] COG0441 Threonyl-tRNA synthetase Length = 346 Score = 59.7 bits (143), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 0/154 (0%), Positives = 0/154 (0%), Gaps = 5/154 (3%) Query: 19 AEAETVNHQLLLRAG---YIRQLAAGIYTYLPLGRKVLRKIEAIVREEMDAAGAQELLMP 75 + L L +E + ++ Sbjct: 284 HGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTY 343 Query: 76 SMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRGEISSYRKLPITV 135 Sbjct: 344 - 402 Query: 136 YQIGTKFRDERR-PRSGLMRGREFLMKDAYSFDT 168 Sbjct: 403 436 >STM0740 [C] COG1271 Cytochrome bd-type quinol oxidase, subunit 1 Length = 522 Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 8/162 (4%), Positives = 29/162 (17%), Gaps = 3/162 (1%) Query: 37 QLAAGIYTYLPLGRKVLRKIEAIVREEMDAAGAQELLMPSMQPAELWKESERYEVYGKEL 96 A P + + +E A L + ++ + Sbjct: 256 IEAEWETQPAPASFTLFGIPDQDKQENHLAIQIPYALGIIATRSVDTPVIGLKDLMV-QH 314 Query: 97 MKLSDRHDREFVLGPTHEEVITSLMRGEISSYRKLPITVYQIGTKFRDERR-PRSGLMRG 155 + + + L T + + K + + ++ ++ Sbjct: 315 EERIRNGMKAYELLEQLRAGSTDQAVRDQFNSMKKDLGYGLLLKRYTPNVTDATEAQIQQ 374 Query: 156 REFLMKDAYSFDTGWEGLDDTYSRMYKAYQRIFERCGLVYRA 197 + ++ Sbjct: 375 ATKDSIPRVAPLYF-AFRIMVACGFLLLAIIALSFWSVIRNR 415 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,268,798 Number of extensions: 1440407 Number of successful extensions: 3862 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 3855 Number of HSP's successfully gapped: 15 Length of query: 488 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 378 Effective length of database: 25,691,691 Effective search space: 9711459198 Effective search space used: 9711459198 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)