BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0058.p (360 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.07_4 [ER] COG0493 NADPH-dependent glutamate synthase be... 276 3e-74 Rv3858c [ER] COG0493 NADPH-dependent glutamate synthase beta cha... 258 8e-69 MT3973 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 258 8e-69 YDL171c_4 [ER] COG0493 NADPH-dependent glutamate synthase beta c... 257 2e-68 VC2374 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 243 4e-64 >SPAPB1E7.07_4 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 276 bits (707), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 0/325 (0%), Positives = 0/325 (0%), Gaps = 11/325 (3%) Query: 40 GFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDMSGGTS 99 +R ++ G Sbjct: 4 WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAA------- 56 Query: 100 GCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXX--XXXXXXXXXXXXXXI 157 + P L + Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 158 KTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTVFERSD 217 ++ + P + ++ Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 218 RIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGK--DIPAQQLVDEYDAVVL 275 I Y L ++ + Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 276 CGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGGGDT 335 Sbjct: 237 296 Query: 336 GSDCVATALRHGCSSITQFGTXXXP 360 Sbjct: 297 321 >Rv3858c [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 258 bits (660), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 0/332 (0%), Positives = 0/332 (0%), Gaps = 15/332 (4%) Query: 35 MSTPTGFMEY-KRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGID 93 MS + + E ++ + + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAA---- 56 Query: 94 MSGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXX--XXXXXXXXXXXXX 151 + + + +E Sbjct: 57 -----RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYE 111 Query: 152 XXXXXIKTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVT 211 + + + + Sbjct: 112 LFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSE 171 Query: 212 VFERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYD 271 D + +P+ + V + + K Sbjct: 172 AGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPL 231 Query: 272 AVVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKL--EDGN-YVSAAGKDVI 328 Sbjct: 232 291 Query: 329 VLGGGDTGSDCVATALRHGCSSITQFGTXXXP 360 Sbjct: 292 323 >MT3973 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 258 bits (660), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 0/332 (0%), Positives = 0/332 (0%), Gaps = 15/332 (4%) Query: 35 MSTPTGFMEY-KRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGID 93 MS + + E ++ + + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAA---- 56 Query: 94 MSGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXX--XXXXXXXXXXXXX 151 + + + +E Sbjct: 57 -----RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYE 111 Query: 152 XXXXXIKTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVT 211 + + + + Sbjct: 112 LFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSE 171 Query: 212 VFERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYD 271 D + +P+ + V + + K Sbjct: 172 AGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPL 231 Query: 272 AVVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKL--EDGN-YVSAAGKDVI 328 Sbjct: 232 291 Query: 329 VLGGGDTGSDCVATALRHGCSSITQFGTXXXP 360 Sbjct: 292 323 >YDL171c_4 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 257 bits (657), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 0/323 (0%), Positives = 0/323 (0%), Gaps = 10/323 (3%) Query: 40 GFMEYKRQLPGDRDPAQRVKDWEEFHHHLTEDELRTQGARCMDCGTPYCHTGIDMSGGTS 99 +R ++ G + Sbjct: 4 WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWP-------AA 56 Query: 100 GCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXX--XXXXXXXXXXXXXXI 157 + P L + Sbjct: 57 RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHL 116 Query: 158 KTIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVTVFERSD 217 ++ + P + ++ Sbjct: 117 VSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEV 176 Query: 218 RIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYDAVVLCG 277 I Y L ++ Q L ++Y Sbjct: 177 DIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 Query: 278 GATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLGGGDTGS 337 Sbjct: 237 - 295 Query: 338 DCVATALRHGCSSITQFGTXXXP 360 Sbjct: 296 318 >VC2374 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 243 bits (620), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 0/329 (0%), Positives = 0/329 (0%), Gaps = 13/329 (3%) Query: 35 MSTPTGFMEYKRQLPGDRDPAQRVKDWEEF-HHHLTEDELRTQGARCMDCGTPYCHTGID 93 MS + R + + ++ + + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAA---- 56 Query: 94 MSGGTSGCPVHNLIPEWNNLVYRGLWKEALERLXXXXXXXXXXXXXXXXXXXXXXXXXXX 153 + + + +E Sbjct: 57 -----RITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYE 111 Query: 154 XXXIK--TIELAIIDKGFEEGWVVPNPPEKRTGKTVAIVXXXXXXXXXXXXXXXXXHTVT 211 + + + Sbjct: 112 LFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSE 171 Query: 212 VFERSDRIGGLLTYGIPTMKLDKRVVQRRVDLLAAEGVKFVVNTEIGKDIPAQQLVDEYD 271 D + +P+ + V + + K Sbjct: 172 AGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPL 231 Query: 272 AVVLCGGATKARRFNVEGSELNGVMYAMDYLNGTIKSYLNSKLEDGNYVSAAGKDVIVLG 331 Sbjct: 232 - 290 Query: 332 GGDTGSDCVATALRHGCSSITQFGTXXXP 360 Sbjct: 291 319 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,937,096 Number of extensions: 914625 Number of successful extensions: 8384 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 8277 Number of HSP's successfully gapped: 71 Length of query: 360 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 252 Effective length of database: 25,922,359 Effective search space: 6532434468 Effective search space used: 6532434468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)