BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0476.0056.p
         (78 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BS_thyA [F] COG0207 Thymidylate synthase                              126   1e-29
BS_thyB [F] COG0207 Thymidylate synthase                               84   8e-17
BS_thyA [F] COG0207 Thymidylate synthase                               71   4e-13
ECU01g1430 [F] COG0207 Thymidylate synthase                            64   6e-11
ECU01g0180 [F] COG0207 Thymidylate synthase                            64   6e-11

>BS_thyA [F] COG0207 Thymidylate synthase
          Length = 264

 Score =  126 bits (316), Expect = 1e-29,   Method: Compositional matrix adjust.
 Identities = 54/78 (69%), Positives = 67/78 (85%)

Query: 1   MVAQQTGLEVGEFIWSGGDVHIYTNHLEQVATQLAREPYPLPKLVIRRQPESIFDYTFED 60
           M+AQQ  LEVG+F+W+GGD H+Y+NH++Q   QL+REP PLPKL+I+R+PESIFDY FED
Sbjct: 187 MMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSREPRPLPKLIIKRKPESIFDYRFED 246

Query: 61  FEFVGYEHHPGIKAPVAI 78
           FE  GY+ HPGIKAPVAI
Sbjct: 247 FEIEGYDPHPGIKAPVAI 264


>BS_thyB [F] COG0207 Thymidylate synthase
          Length = 264

 Score = 83.6 bits (205), Expect = 8e-17,   Method: Compositional matrix adjust.
 Identities = 41/78 (52%), Positives = 54/78 (69%)

Query: 1   MVAQQTGLEVGEFIWSGGDVHIYTNHLEQVATQLAREPYPLPKLVIRRQPESIFDYTFED 60
           ++A  TGLE GEFI + GDVHIY NH+EQV  QL R+  PLP+L   R+ +SIF++ FED
Sbjct: 187 IIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIFNFAFED 246

Query: 61  FEFVGYEHHPGIKAPVAI 78
           F    Y+ HP IK  V++
Sbjct: 247 FIIEDYDPHPHIKGAVSV 264



 Score = 70.9 bits (172), Expect = 4e-13,   Method: Composition-based stats.
 Identities = 0/78 (0%), Positives = 0/78 (0%)

Query: 1   MVAQQTGLEVGEFIWSGGDVHIYTNHLEQVATQLAREPYPLPKLVIRRQPESIFDYTFED 60
                                      + +  +     +         +           
Sbjct: 202 TGGDTHLYSNHMDQTHLQLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAP 261

Query: 61  FEFVGYEHHPGIKAPVAI 78
                             
Sbjct: 262                    279


>ECU01g1430 [F] COG0207 Thymidylate synthase
          Length = 294

 Score = 63.9 bits (154), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 33/82 (40%), Positives = 49/82 (59%), Gaps = 8/82 (9%)

Query: 1   MVAQQTGLEVGEFIWSGGDVHIYTNHLEQVATQLAREPYPLPKLVI-----RRQPESIFD 55
           +VA  TGL+ GEF+   GD H+Y +H++ +  Q+ R P   PKL +     R +PE    
Sbjct: 215 LVAHLTGLQPGEFVHFLGDAHVYLDHVDSLRQQIQRPPRAFPKLFVSPKGPRTEPEH--- 271

Query: 56  YTFEDFEFVGYEHHPGIKAPVA 77
           + +EDFE VGY+ HP IK  ++
Sbjct: 272 FQYEDFELVGYDPHPAIKMNMS 293


>ECU01g0180 [F] COG0207 Thymidylate synthase
          Length = 294

 Score = 63.9 bits (154), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 33/82 (40%), Positives = 49/82 (59%), Gaps = 8/82 (9%)

Query: 1   MVAQQTGLEVGEFIWSGGDVHIYTNHLEQVATQLAREPYPLPKLVI-----RRQPESIFD 55
           +VA  TGL+ GEF+   GD H+Y +H++ +  Q+ R P   PKL +     R +PE    
Sbjct: 215 LVAHLTGLQPGEFVHFLGDAHVYLDHVDSLRQQIQRPPRAFPKLFVSPKGPRTEPEH--- 271

Query: 56  YTFEDFEFVGYEHHPGIKAPVA 77
           + +EDFE VGY+ HP IK  ++
Sbjct: 272 FQYEDFELVGYDPHPAIKMNMS 293


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.321    0.141    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 6,377,208
Number of extensions: 191475
Number of successful extensions: 329
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 326
Number of HSP's successfully gapped: 6
Length of query: 78
Length of database: 38,378,431
Length adjustment: 49
Effective length of query: 29
Effective length of database: 32,727,065
Effective search space: 949084885
Effective search space used: 949084885
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 109 (46.6 bits)