BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0054.p (263 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3805 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 140 3e-33 BH2719 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 134 1e-31 STM3079 [R] COG0596 Predicted hydrolases or acyltransferases (al... 77 2e-14 lin2547 [R] COG0596 Predicted hydrolases or acyltransferases (al... 72 6e-13 BMEI1951 [R] COG0596 Predicted hydrolases or acyltransferases (a... 70 3e-12 >BH3805 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 259 Score = 140 bits (352), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 79/247 (31%), Positives = 135/247 (54%), Gaps = 5/247 (2%) Query: 11 MHYTDCGQGTTIIFIHPPVLTSLNFLYQMKPLSADFRTISFDIRGHGSSKSSREEITYPL 70 ++Y G+GT I+FI PP + L F Q++ L++ F+ I++D+RG+G S+++ E +T Sbjct: 11 LYYEVSGEGTAILFIPPPAMGHLTFHEQVRGLTSCFQVITYDLRGNGRSETTDEPLTLEQ 70 Query: 71 IVEDIRQLMDQLKIEKAFLCGYSTGGSIVLDFLLTCPERAFGGIVISGMSEVNDKKLRNK 130 + ED++ ++D L +E++ +CGYS G I F L P+R G + ISG SEV+ L+ + Sbjct: 71 MAEDVKAILDDLGVEQSIVCGYSNGALIAQLFALMYPDRCLGVVSISGFSEVSPLLLKKQ 130 Query: 131 IARGRFL--SRXXXXXXXXLSISWSQAKARLSLFRTLFTGAKRANAENAEQYYHYSMQYN 188 G + S L+I+ Q K + + + K+++ EQ Y Q N Sbjct: 131 YDLGVSVTGSEHLDFFAWVLAIAHFQDK---KVRKEIQKEIKKSDPHMIEQQYAVGKQGN 187 Query: 189 CTAQLDRIHHPVLLVYGEKDTLFHPYAKLLQERLPKSELVFIKKTKHQIPTKAAGKVNGL 248 T L ++ P+L++YG+ D H + + + ELV+I + HQIPT ++NG+ Sbjct: 188 VTRALHQLTVPLLVLYGDHDYYIHSTKRPFIDHVKDLELVYIDGSTHQIPTHHPAELNGI 247 Query: 249 IRQFIHR 255 IR + R Sbjct: 248 IRSWTKR 254 >BH2719 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 260 Score = 134 bits (338), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 79/252 (31%), Positives = 131/252 (51%), Gaps = 14/252 (5%) Query: 10 SMHYTDCGQGTTIIFIHPPVLTSLNFLYQMKPLSADFRTISFDIRGHGSSKSSREEITYP 69 +++Y G+GT IIFI PP + L F YQ+ PL + I+FD+RG S+ S E +T Sbjct: 11 AIYYETIGKGTPIIFIPPPGVGHLTFRYQV-PLMNQCKLITFDVRGDCQSERSTEPMTMT 69 Query: 70 LIVEDIRQLMDQLKIEKAFLCGYSTGGSIVLDFLLTCPERAFGGIVISGMSEVNDKKLRN 129 + D+++++D + KA +CGYS G +I +F LT PER G I+I G V + L Sbjct: 70 QLAYDVKRVLDANCVRKAIVCGYSNGAAIAQEFALTYPERTAGVILIGGYFAVKNFLLEK 129 Query: 130 KIARGRFLSRXXXXXXXXLSIS---WSQAKARLSLFRTLFTGAKRANAENAEQYYHYSMQ 186 + G + +R ++ ++ +A L+ + + A+ HYS + Sbjct: 130 EYKLGIWAARNELMPLLASGLAKNHFTDKQAANELYAEIMKTDPQMLADQYTTGLHYSSE 189 Query: 187 YNCTAQLDRIHH---PVLLVYGEKDTLFHPYAKLLQERLPKSELVFIKKTKHQIPTKAAG 243 DR+H P+LL+YG D PY +E + E+V+I+ KHQ+P+KA Sbjct: 190 -------DRLHQLRVPLLLIYGANDHYLQPYQTRFRELVHDVEIVYIQGVKHQVPSKAPH 242 Query: 244 KVNGLIRQFIHR 255 + N ++R+++ R Sbjct: 243 ECNAVLREWMRR 254 >STM3079 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 318 Score = 77.4 bits (189), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 70/270 (25%), Positives = 112/270 (41%), Gaps = 35/270 (12%) Query: 7 NGYSMHYTDCGQGTTIIFIHPPVLTSLNFLYQMKPLSADFRTISFDIRGHGSSKSSREEI 66 NG +HY + G+G ++ IH LT+ ++ K S FR I D RGHG + + + Sbjct: 57 NGIKIHYVEEGEGPPLLLIHGGGLTAKSWQGLAKEASRYFRVIMPDSRGHGLTNNPQGTF 116 Query: 67 TYPLIVEDIRQLMDQLKIEKAFLCGYSTGGSIVLDFLLTCPERAFGGIV----------- 115 +Y L+ ED+ + LK+EK + GYS GG +VL P+ A IV Sbjct: 117 SYDLMTEDMAAFVKALKLEKPLVMGYSDGGMVVLKLTSRYPDLARAAIVGGATHRFATTH 176 Query: 116 -ISGMSEVNDKKLRNKIARGRFLSRXXXXXXXXLSISWS-QAKARLSLFRTLFTGAKRAN 173 + GM K + R L + + + + +RT G Sbjct: 177 YMQGMEIFYGKGMPQGQLTDRDLDKMASDAPGMVKFYQNMHHPEQKDYWRTFLKGV---- 232 Query: 174 AENAEQYYHYSMQYNCTA----QLDRIHHPVLLVYGEKDTLFH-PYAKLLQERLPKSELV 228 + M T+ ++ +IH PVLL+ G++D F L LP++E+ Sbjct: 233 ---------WPMWTTPTSLAEEEVKKIHIPVLLLDGDRDEFFTVEEVTELYRLLPQAEMT 283 Query: 229 FIKKTKHQIPTKAAGKVN---GLIRQFIHR 255 I + H I + GK L+ +F+ R Sbjct: 284 LIPGSGHAI-FQTPGKTPLFYALVLEFLQR 312 >lin2547 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 301 Score = 72.4 bits (176), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 11/247 (4%), Positives = 39/247 (15%), Gaps = 22/247 (8%) Query: 6 INGYSMHYTDCGQGTTIIFIHPPVLTSLNFLYQMKPLSADFRTISFDIRGHGSSKSSREE 65 + + +L + K + R + G + Sbjct: 5 KSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTP 64 Query: 66 ITYPLIVEDIRQLMDQLKIEKAFLCGYSTGGSIVLDFLLTCPERAFGGIVISGMSEVNDK 125 + I +LM++ + + Sbjct: 65 GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILA-- 122 Query: 126 KLRNKIARGRFLSRXXXXXXXXLSISWSQAKARLSLFRTLFTGAKRANAENAEQYYHYSM 185 + + + L+ ++ Sbjct: 123 -------VNKVDN------VQEKADLLP-HLQFLASQMNFL---DIVPISAETGLNVDTI 165 Query: 186 QYNCTAQLDRIHHPVLLVYGEKDTLFHPYAKLLQERLPKSELVFIKKTKHQIPTKAAGKV 245 + + T + K + + + + V Sbjct: 166 ---AAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFV 222 Query: 246 NGLIRQF 252 + + Sbjct: 223 SNERGGY 229 >BMEI1951 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 233 Score = 70.1 bits (170), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 41/110 (37%), Positives = 57/110 (51%), Gaps = 2/110 (1%) Query: 2 PQAF--INGYSMHYTDCGQGTTIIFIHPPVLTSLNFLYQMKPLSADFRTISFDIRGHGSS 59 P AF +NG M+Y G+G I+ IH + + Q+ L+ I D RG G S Sbjct: 38 PAAFAEVNGIEMYYRVVGKGPPILLIHGGLSDQHVWDAQLPILARHHSVIVADSRGQGRS 97 Query: 60 KSSREEITYPLIVEDIRQLMDQLKIEKAFLCGYSTGGSIVLDFLLTCPER 109 + E ITY L+ +D L+D L I+K L G+S GG I LD + P+R Sbjct: 98 TRTDEPITYRLMADDYVALLDYLHIDKVDLVGWSDGGIIGLDIAMRYPDR 147 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,702,335 Number of extensions: 636476 Number of successful extensions: 2061 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 2068 Number of HSP's successfully gapped: 27 Length of query: 263 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 159 Effective length of database: 26,383,695 Effective search space: 4195007505 Effective search space used: 4195007505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)