BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0053.p (470 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pdhD [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 558 e-159 BH2652 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 547 e-155 SA0946 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex... 540 e-153 lin1047 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase comple... 538 e-153 L0036 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex,... 455 e-128 >BS_pdhD [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 558 bits (1438), Expect = e-159, Method: Compositional matrix adjust. Identities = 24/471 (5%), Positives = 102/471 (21%), Gaps = 2/471 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 M+ + + +V+G + + + +G + S + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 + ++ + + N + + K Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 + + + + + + + + AL ++ + ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTG-IEIVTNAK 239 Q+ V I+ + + ++ + + Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 240 AESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKV 299 + + + + + T Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGT-REIAAGNTDLSSRTEQQASA 299 Query: 300 DHQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPEC 359 + ++ + A A + G K + + + + Sbjct: 300 LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI 359 Query: 360 SSVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIE 419 + + + + A ++ + + +I + Sbjct: 360 ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALI 419 Query: 420 ASNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVVK 470 ++ ++ + + E + ++A + +I+ E A + + + Sbjct: 420 EDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQ 470 >BH2652 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 547 bits (1410), Expect = e-155, Method: Compositional matrix adjust. Identities = 26/470 (5%), Positives = 92/470 (19%), Gaps = 1/470 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 M+ + + +V+G + + + +G + S + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 + ++ + + N + + K Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 + + + + + + + + AL ++ + ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIVTNAKA 240 Q+ V I+ + + ++ + + N Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 241 ESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKVD 300 ++ S S T ++ Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAG-TREIAAGNTDLSSRTEQQASA 299 Query: 301 HQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPECS 360 + + + A G K V + + Sbjct: 300 LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI 359 Query: 361 SVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIEA 420 + ++ + + + V N + A+ + Sbjct: 360 ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALI 419 Query: 421 SNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVVK 470 + ++ + + + + I T +M + Sbjct: 420 EDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGID 469 >SA0946 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 540 bits (1390), Expect = e-153, Method: Compositional matrix adjust. Identities = 26/469 (5%), Positives = 92/469 (19%), Gaps = 1/469 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 M+ + + +V+G + + + +G + S + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 + ++ + + N + + K Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 + + + + + + + + AL ++ + ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIVTNAKA 240 Q+ V I+ + + ++ + + N Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 241 ESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKVD 300 ++ S S T ++ Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAG-TREIAAGNTDLSSRTEQQASA 299 Query: 301 HQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPECS 360 + + + A G K V + + Sbjct: 300 LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI 359 Query: 361 SVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIEA 420 + ++ + + + V N + A+ + Sbjct: 360 ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALI 419 Query: 421 SNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVV 469 + ++ + + + + I T +M + Sbjct: 420 EDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI 468 >lin1047 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 538 bits (1385), Expect = e-153, Method: Compositional matrix adjust. Identities = 26/468 (5%), Positives = 91/468 (19%), Gaps = 1/468 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 M+ + + +V+G + + + +G + S + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 + ++ + + N + + K Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 + + + + + + + + AL ++ + ++ Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIVTNAKA 240 Q+ V I+ + + ++ + + N Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 241 ESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKVD 300 ++ S S T ++ Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAG-TREIAAGNTDLSSRTEQQASA 299 Query: 301 HQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPECS 360 + + + A G K V + + Sbjct: 300 LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI 359 Query: 361 SVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIEA 420 + ++ + + + V N + A+ + Sbjct: 360 ADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALI 419 Query: 421 SNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHV 468 + ++ + + + + I T +M Sbjct: 420 EDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRG 467 >L0036 [C] COG1249 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes Length = 553 Score = 455 bits (1171), Expect = e-128, Method: Compositional matrix adjust. Identities = 36/474 (7%), Positives = 102/474 (21%), Gaps = 8/474 (1%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 M+ + + +V+G + + + +G + S + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLM 60 Query: 61 AAHQYEAA---QHGE-VFGVTAENVKVDWAKTQEFK-NGVVKRMTTGVTSLMKGNKIEVF 115 + + N KV+ + + + Sbjct: 61 LQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATS 120 Query: 116 SGEAMFISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEI 175 L + Y A ++ ++ + S+E L + Sbjct: 121 RNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV 180 Query: 176 PKSMIVIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIV 235 + + + V +L K + + + Sbjct: 181 TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 236 TNAKAESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRG 295 + ++E ++ S+ V + G R Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIA-AGNTDLSSRTEQQASA 299 Query: 296 LIKVDHQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKV-IPAVVF 354 L + + D + +++ + + +++ + Sbjct: 300 LEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKI 359 Query: 355 TDPECSSVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQ 414 D G+ + GR ++ + E +++ + A Sbjct: 360 ADIISVIDGIAFQTNILA-LNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKAL 418 Query: 415 IVGIEASNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHV 468 I + + + + +GEI + E G Sbjct: 419 IEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVA 472 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,385,653 Number of extensions: 1372154 Number of successful extensions: 5600 Number of sequences better than 1.0e-05: 206 Number of HSP's gapped: 4776 Number of HSP's successfully gapped: 206 Length of query: 470 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 360 Effective length of database: 25,691,691 Effective search space: 9249008760 Effective search space used: 9249008760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)