BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0476.0052.p
         (63 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SA0945 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex...   100   4e-22
lin1046 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple...   100   8e-22
BH2653 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex...    96   1e-20
BS_pdhC [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple...    96   1e-20
BH0778 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex...    69   1e-12

>SA0945 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex,
           dihydrolipoamide acyltransferase (E2) component, and
           related enzymes
          Length = 553

 Score =  100 bits (250), Expect = 4e-22,   Method: Compositional matrix adjust.
 Identities = 5/62 (8%), Positives = 15/62 (24%)

Query: 1   VAILGTGRITEKPVVKNGEIVAAPVMALSLSFDHRIIDGATAQNFMNYIKTLLANPDMLV 60
                T  +     V+             ++ + R +   +AQ        +  +   + 
Sbjct: 368 GIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVD 427

Query: 61  ME 62
             
Sbjct: 428 TG 429


>lin1046 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex,
           dihydrolipoamide acyltransferase (E2) component, and
           related enzymes
          Length = 553

 Score =  100 bits (248), Expect = 8e-22,   Method: Compositional matrix adjust.
 Identities = 3/63 (4%), Positives = 10/63 (15%)

Query: 1   VAILGTGRITEKPVVKNGEIVAAPVMALSLSFDHRIIDGATAQNFMNYIKTLLANPDMLV 60
                +            E  A+ +     +F              +   +        +
Sbjct: 482 TQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRL 541

Query: 61  MEV 63
              
Sbjct: 542 RIA 544


>BH2653 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex,
           dihydrolipoamide acyltransferase (E2) component, and
           related enzymes
          Length = 553

 Score = 95.9 bits (237), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 8/62 (12%), Positives = 11/62 (17%)

Query: 1   VAILGTGRITEKPVVKNGEIVAAPVMALSLSFDHRIIDGATAQNFMNYIKTLLANPDMLV 60
             I G    T    +      A                   A       K + A  +  V
Sbjct: 364 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSV 423

Query: 61  ME 62
             
Sbjct: 424 SR 425


>BS_pdhC [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex,
           dihydrolipoamide acyltransferase (E2) component, and
           related enzymes
          Length = 553

 Score = 95.9 bits (237), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 5/62 (8%), Positives = 11/62 (17%)

Query: 1   VAILGTGRITEKPVVKNGEIVAAPVMALSLSFDHRIIDGATAQNFMNYIKTLLANPDMLV 60
            A+       +             + + S      I              ++L       
Sbjct: 380 AAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGET 439

Query: 61  ME 62
           M 
Sbjct: 440 MN 441


>BH0778 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex,
           dihydrolipoamide acyltransferase (E2) component, and
           related enzymes
          Length = 553

 Score = 69.3 bits (168), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 6/60 (10%), Positives = 13/60 (21%)

Query: 2   AILGTGRITEKPVVKNGEIVAAPVMALSLSFDHRIIDGATAQNFMNYIKTLLANPDMLVM 61
           A+              G  V A  +    S   +      A    +  +    +  +   
Sbjct: 377 ALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESA 436


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.322    0.137    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 4,552,920
Number of extensions: 126579
Number of successful extensions: 319
Number of sequences better than 1.0e-05: 72
Number of HSP's gapped: 326
Number of HSP's successfully gapped: 72
Length of query: 63
Length of database: 38,378,431
Length adjustment: 35
Effective length of query: 28
Effective length of database: 34,341,741
Effective search space: 961568748
Effective search space used: 961568748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 109 (46.6 bits)