BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0050.p (356 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1046 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 132 6e-31 SA0945 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 114 2e-25 BH2653 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 107 4e-23 BS_pdhC [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 97 4e-20 PA3415 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 78 2e-14 >lin1046 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 132 bits (333), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 7/280 (2%), Positives = 26/280 (9%), Gaps = 20/280 (7%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 R + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 64 DGQVCRVGEVVAIIDAEGDIPEQEGGHXXXXXXXXXXXXKGGXXXXXXXXXXXXXNFEYK 123 V ++D+ Sbjct: 63 TRINLSRSAVRMMMDS------SNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEM 116 Query: 124 FPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAKDGQV 183 + D + + + Sbjct: 117 VATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLY 176 Query: 184 CRVGEVVAIIAAEGDV------------PQQEGGHXXXXXXXXXXXXXXXXXXXXXXXNR 231 + Sbjct: 177 RDIVTD--NADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGN 234 Query: 232 DVLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 + +TH + Sbjct: 235 LANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDA 274 >SA0945 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 114 bits (286), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 5/160 (3%), Positives = 22/160 (13%), Gaps = 8/160 (5%) Query: 120 FEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAK 179 + + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 180 DGQVCRVGEVVAIIAAEGDVPQQEGGHXXXXXXXX--------XXXXXXXXXXXXXXXNR 231 V ++ + + Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 232 DVLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 + + + N + T A Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMG 162 Score = 77.4 bits (189), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 4/76 (5%), Positives = 15/76 (19%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 R + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 64 DGQVCRVGEVVAIIDA 79 V ++D+ Sbjct: 63 TRINLSRSAVRMMMDS 78 >BH2653 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 107 bits (266), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 7/159 (4%), Positives = 31/159 (19%), Gaps = 7/159 (4%) Query: 120 FEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAK 179 + + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 180 DGQVCRVGEV-VAIIAAEG------DVPQQEGGHXXXXXXXXXXXXXXXXXXXXXXXNRD 232 V + + ++ ++ +R+ Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 233 VLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 + A + +D TG + ++ + Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAM 161 Score = 74.7 bits (182), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 4/76 (5%), Positives = 15/76 (19%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 R + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 64 DGQVCRVGEVVAIIDA 79 V ++D+ Sbjct: 63 TRINLSRSAVRMMMDS 78 >BS_pdhC [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 97.1 bits (240), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 5/176 (2%), Positives = 23/176 (13%), Gaps = 24/176 (13%) Query: 120 FEYKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAK 179 + + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 180 DGQVCRVGEVVAIIAAEGDVPQQ--------------------EGGHXXXXXXXXXXXXX 219 V ++ + + Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 220 XXXXXXXXXX----NRDVLATPSVRKFARDQSVDISKVNGTGKNGKITHEDVEAFL 271 D L + + + + G + Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD 178 Score = 70.5 bits (171), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 4/78 (5%), Positives = 15/78 (19%) Query: 4 FEYRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTK 63 R + I + + + + ++ + Sbjct: 3 NRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQ 62 Query: 64 DGQVCRVGEVVAIIDAEG 81 V ++D+ Sbjct: 63 TRINLSRSAVRMMMDSSN 80 >PA3415 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Length = 553 Score = 77.8 bits (190), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 4/151 (2%), Positives = 18/151 (11%), Gaps = 7/151 (4%) Query: 122 YKFPXXXXXXXXXXIIKMHIKAGDKITDDDIIMEVQNDKAVVEVPSPVNGTVLEVFAKDG 181 + + +G + + T Sbjct: 4 RIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQT 63 Query: 182 QVCRVGEVVAIIAAEGDVPQQEGGHXXXXXXXXXXXXXXXXXXXXXXXNRDVL---ATPS 238 ++ A+ + + + Sbjct: 64 RINLSRS--AVRMMMDS--SNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVAT 119 Query: 239 VRKFARDQSVDISKVNGTGKNGKITHEDVEA 269 R + + + Sbjct: 120 SRNIDEKYKNYYTALTELIDYLDYGNTGAYF 150 Score = 50.8 bits (120), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 3/75 (4%), Positives = 12/75 (16%) Query: 6 YRFPXXXXXXXXXXIIKMHIKAGDKVTDDDIVMEVQNDKAVVEVPCPVNGTVLEVFTKDG 65 + + +G + + T Sbjct: 4 RIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQT 63 Query: 66 QVCRVGEVVAIIDAE 80 ++ V ++ Sbjct: 64 RINLSRSAVRMMMDS 78 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,341,691 Number of extensions: 608925 Number of successful extensions: 1742 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 1728 Number of HSP's successfully gapped: 62 Length of query: 356 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 249 Effective length of database: 26,037,693 Effective search space: 6483385557 Effective search space used: 6483385557 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)