BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0049.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pdhB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 371 e-103 BH2654 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 364 e-101 lin1045 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase comple... 362 e-100 SA0944 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 349 3e-96 BH0214 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex... 262 3e-70 >BS_pdhB [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 371 bits (952), Expect = e-103, Method: Compositional matrix adjust. Identities = 15/251 (5%), Positives = 41/251 (16%) Query: 23 FRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTDSLE 82 + Q + A + AP+ + + + Sbjct: 75 MMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTAL 134 Query: 83 GLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTVELG 142 + +G+ A + + Sbjct: 135 TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQL 194 Query: 143 KANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPIDIDTIVASVKKT 202 + + + YG+ T A+ + A T++ + + Sbjct: 195 AVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSV 254 Query: 203 NRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIPTPAR 262 + + + I + T Sbjct: 255 SHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATV 314 Query: 263 VIAAVKKVMEF 273 A Sbjct: 315 KQNADNARQAS 325 >BH2654 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 364 bits (935), Expect = e-101, Method: Compositional matrix adjust. Identities = 15/251 (5%), Positives = 41/251 (16%) Query: 23 FRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTDSLE 82 + Q + A + AP+ + + + Sbjct: 75 MMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTAL 134 Query: 83 GLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTVELG 142 + +G+ A + + Sbjct: 135 TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQL 194 Query: 143 KANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPIDIDTIVASVKKT 202 + + + YG+ T A+ + A T++ + + Sbjct: 195 AVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSV 254 Query: 203 NRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIPTPAR 262 + + + I + T Sbjct: 255 SHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATV 314 Query: 263 VIAAVKKVMEF 273 A Sbjct: 315 KQNADNARQAS 325 >lin1045 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 362 bits (930), Expect = e-100, Method: Compositional matrix adjust. Identities = 16/262 (6%), Positives = 43/262 (16%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 V+ + Q + A + AP+ + Sbjct: 64 RINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNI 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 + + + +G+ A Sbjct: 124 DEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDN 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 + + + + + YG+ T A+ + A T++ Sbjct: 184 ADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDG 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 + + + + + I + Sbjct: 244 RSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEET 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 T A Sbjct: 304 AASMEQLTATVKQNADNARQAS 325 >SA0944 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 349 bits (895), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 16/262 (6%), Positives = 43/262 (16%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 V+ + Q + A + AP+ + Sbjct: 64 RINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNI 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 + + + +G+ A Sbjct: 124 DEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDN 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 + + + + + YG+ T A+ + A T++ Sbjct: 184 ADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDG 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 + + + + + I + Sbjct: 244 RSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEET 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 T A Sbjct: 304 AASMEQLTATVKQNADNARQAS 325 >BH0214 [C] COG0022 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit Length = 553 Score = 262 bits (670), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 27/255 (10%), Positives = 54/255 (21%), Gaps = 1/255 (0%) Query: 20 VQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTD 79 + + + ++ + + QAA + P + T K +T Sbjct: 74 MMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTA 133 Query: 80 SLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTV 139 E + G + P + + + A+ Sbjct: 134 LTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQ 193 Query: 140 ELGKANVVREGSDVSIITYGLMVHTATKAAEQLEK-DGIKAEIIDLRTVSPIDIDTIVAS 198 A VV V+ M+ T + L ++ + S Sbjct: 194 LAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQS 253 Query: 199 VKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIP 258 V R++ G A A + A +E Sbjct: 254 VSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTAT 313 Query: 259 TPARVIAAVKKVMEF 273 A + Sbjct: 314 VKQNADNARQASQLA 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,026,694 Number of extensions: 822496 Number of successful extensions: 1740 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 1699 Number of HSP's successfully gapped: 35 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)