BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0048.p (358 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2655 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 400 e-111 BS_pdhA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 390 e-108 lin1044 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 378 e-105 BH0213 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 252 8e-67 VNG2217G [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase compl... 236 3e-62 >BH2655 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 400 bits (1029), Expect = e-111, Method: Compositional matrix adjust. Identities = 17/342 (4%), Positives = 55/342 (16%), Gaps = 1/342 (0%) Query: 17 EALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRLGFYAPVSG 76 + + + ++ + +L Q R+ Sbjct: 14 VLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVR 73 Query: 77 QEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPEGVNVLMP 136 + + E + A L + N Sbjct: 74 MMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTA 133 Query: 137 QIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFKLPVIFFVQ 196 + + + A + + A+ + + Q Sbjct: 134 LTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQ 193 Query: 197 NNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXXXXXXXXTL 256 A+ I ++ Sbjct: 194 LAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQS 253 Query: 257 IEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEEDTLRVREE 315 + + + S ++ + T ++ Sbjct: 254 VSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTAT 313 Query: 316 AKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQKADF 357 K + + ++ + + + AD Sbjct: 314 VKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADS 355 >BS_pdhA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 390 bits (1002), Expect = e-108, Method: Compositional matrix adjust. Identities = 15/350 (4%), Positives = 59/350 (16%), Gaps = 1/350 (0%) Query: 5 TKVPYEVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGR 64 V + + S+ K + + + + + ++ Sbjct: 12 VMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSA 71 Query: 65 QGRLGFYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHG 124 + + + + + + + + Sbjct: 72 VRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYY 131 Query: 125 GQIPEGVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFA 184 + E ++ L G+ Q + + + Sbjct: 132 TALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQ 191 Query: 185 GRFKLPVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXX 244 + + + V A G + + + Sbjct: 192 WQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLA 251 Query: 245 XXXXXXXXXXTLIEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGL 303 T + + + + ++ ++ Sbjct: 252 QSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLT 311 Query: 304 WTEEDTLRVREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQ 353 T + +A A+ K+ + Sbjct: 312 ATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIAD 361 >lin1044 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 378 bits (970), Expect = e-105, Method: Compositional matrix adjust. Identities = 14/351 (3%), Positives = 58/351 (16%), Gaps = 1/351 (0%) Query: 9 YEVYTEDVEALSVLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRL 68 + + S+ K + + + + + ++ + Sbjct: 16 GVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMM 75 Query: 69 GFYAPVSGQEATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIP 128 + + + + + + + + Sbjct: 76 MDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTALT 135 Query: 129 EGVNVLMPQIIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFK 188 E ++ L G+ Q + + + + Sbjct: 136 ELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLA 195 Query: 189 LPVIFFVQNNGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXX 248 + + V A G + + + Sbjct: 196 VIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVS 255 Query: 249 XXXXXXTLIEAVTYRFRPHSLS-DDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEE 307 T + + + + ++ ++ T + Sbjct: 256 HMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVK 315 Query: 308 DTLRVREEAKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQKADFE 358 +A A+ K+ + Sbjct: 316 QNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVI 366 >BH0213 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 252 bits (643), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 18/342 (5%), Positives = 61/342 (17%), Gaps = 5/342 (1%) Query: 19 LSVLSPDGEVINKDKMPALTDDQLKELMYR-MVFTRTWDDRAVNLGRQGRLGFYAPVSGQ 77 + G + + + L + L A Sbjct: 19 ALLQLISGSL--FFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMMM 76 Query: 78 EATMIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPEGVNVLMPQ 137 +++ S ++ D + ++ + + + + Sbjct: 77 DSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTE 136 Query: 138 IIIGAQILHATGIAMGFKLKKQQNVAITYTGDGGSSEGDFYEGLNFAGRFKLPVIFFVQN 197 +I + Q + + SSE + + + + + Sbjct: 137 LIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAV 196 Query: 198 NGYAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVISAVQXXXXXXXXXXXXTLI 257 + I + + Sbjct: 197 IALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSH 256 Query: 258 EAVTYRFRPHSLSDDASKYRSKEE-EGQWNE-KDPIARLAKYLEKKGLWTEEDTLRVREE 315 + + + + + N ++ + E ++ Sbjct: 257 MQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQ 316 Query: 316 AKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQKADF 357 + + A+ G V H + Sbjct: 317 NADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKK 358 >VNG2217G [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Length = 553 Score = 236 bits (603), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 15/338 (4%), Positives = 58/338 (17%), Gaps = 5/338 (1%) Query: 21 VLSPDGEVINKDKMPALTDDQLKELMYRMVFTRTWDDRAVNLGRQGRLGFYAPVSGQEAT 80 + M + ++ +T A + + + + Sbjct: 63 TRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRN 122 Query: 81 MIGSEFALQKEDFVCPGYRDIPQLVWHGLPLYQAFLYSRGHQHGGQIPEGVNVLMPQIII 140 + Y D + Q + G + + Sbjct: 123 IDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTD 182 Query: 141 GAQILHATGIAMGFKLKKQQNVA-ITYTGDGGSSEGDFYEGLNFAGRFKLPVIFFVQNNG 199 A + + + + G + + + Sbjct: 183 NADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTID 242 Query: 200 YAITTPFAKQTXXXXXXXXXXXXGIPGIKIDGMDVFAVI----SAVQXXXXXXXXXXXXT 255 Q+ D + + Sbjct: 243 GRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEE 302 Query: 256 LIEAVTYRFRPHSLSDDASKYRSKEEEGQWNEKDPIARLAKYLEKKGLWTEEDTLRVREE 315 ++ + D ++ S+ + + ++ + K + + ++ + Sbjct: 303 TAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADI 362 Query: 316 AKATVNEQIKKAEQTEKMTVPGLIDSMFEVTPKHLEEQ 353 + V + + Sbjct: 363 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGE 400 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,433,511 Number of extensions: 1039347 Number of successful extensions: 3001 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 2947 Number of HSP's successfully gapped: 37 Length of query: 358 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 250 Effective length of database: 25,922,359 Effective search space: 6480589750 Effective search space used: 6480589750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)