BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0043.p (168 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value spr [M] COG0791 Cell wall-associated hydrolases (invasion-associ... 99 3e-21 STM2214 [M] COG0791 Cell wall-associated hydrolases (invasion-as... 99 4e-21 YPO1275 [M] COG0791 Cell wall-associated hydrolases (invasion-as... 99 4e-21 HI1652 [M] COG0791 Cell wall-associated hydrolases (invasion-ass... 95 6e-20 BS_ywtD [M] COG0791 Cell wall-associated hydrolases (invasion-as... 87 1e-17 >spr [M] COG0791 Cell wall-associated hydrolases (invasion-associated proteins) Length = 188 Score = 99.0 bits (245), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 52/124 (41%), Positives = 76/124 (61%), Gaps = 8/124 (6%) Query: 46 QIVNLGKKYMGVRYEFGAPTSSTRTFDCSSFTKYIF-GKYGVNLPRTSSAQSKVGKSVSK 104 +I++ + GVRY G S+ + DCS F + F ++G+ LPR++ Q ++GKSVS+ Sbjct: 69 RIMDQYADWKGVRYRLGG--STKKGIDCSGFVQRTFREQFGLELPRSTYEQQEMGKSVSR 126 Query: 105 ANLKVGDLVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGNWKRTYLKA 164 +NL+ GDLV F +GS HV +YIGN + +H S GV IS +N WK+ Y +A Sbjct: 127 SNLRTGDLVLFRAGSTGR-----HVGIYIGNNQFVHASTSSGVIISSMNEPYWKKRYNEA 181 Query: 165 RRVL 168 RRVL Sbjct: 182 RRVL 185 >STM2214 [M] COG0791 Cell wall-associated hydrolases (invasion-associated proteins) Length = 190 Score = 98.6 bits (244), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 52/124 (41%), Positives = 76/124 (61%), Gaps = 8/124 (6%) Query: 46 QIVNLGKKYMGVRYEFGAPTSSTRTFDCSSFTKYIF-GKYGVNLPRTSSAQSKVGKSVSK 104 +I++ + GVRY G S+ + DCSSF + F ++G+ LPR++ Q ++GK+VS+ Sbjct: 71 RIMDQYADWKGVRYRLGG--STKKGVDCSSFVQRTFREQFGLELPRSTYEQQEMGKAVSR 128 Query: 105 ANLKVGDLVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGNWKRTYLKA 164 NL+ GDLV F +GS HV +YIGN + +H S GV IS +N WK+ Y +A Sbjct: 129 NNLRTGDLVLFRAGSTGR-----HVGIYIGNNQFVHASTSSGVIISSMNEPYWKKRYNEA 183 Query: 165 RRVL 168 RRVL Sbjct: 184 RRVL 187 >YPO1275 [M] COG0791 Cell wall-associated hydrolases (invasion-associated proteins) Length = 194 Score = 98.6 bits (244), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 51/116 (43%), Positives = 69/116 (59%), Gaps = 8/116 (6%) Query: 54 YMGVRYEFGAPTSSTRTFDCSSFTKYIF-GKYGVNLPRTSSAQSKVGKSVSKANLKVGDL 112 + GVRY G SS R DCS+F + F ++G++LPR++S Q +GK V + L+ GDL Sbjct: 80 WKGVRYRLGG--SSKRGIDCSAFVQTTFREQFGMDLPRSTSQQQDLGKKVQRTKLRPGDL 137 Query: 113 VFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGNWKRTYLKARRVL 168 V F +GS HV +Y+GN K +H S GVTIS+LN W + Y RRVL Sbjct: 138 VLFRAGSTGR-----HVGIYLGNDKFVHASTSAGVTISNLNDDYWNKRYRDGRRVL 188 >HI1652 [M] COG0791 Cell wall-associated hydrolases (invasion-associated proteins) Length = 183 Score = 94.7 bits (234), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 48/117 (41%), Positives = 71/117 (60%), Gaps = 11/117 (9%) Query: 53 KYMGVRYEFGAPTSSTRTFDCSSFTKYIFGK-YGVNLPRTSSAQSKVGKSVSKANLKVGD 111 +++G RY G T R DCS+F + F + +G+ LPR+++ Q +G+ ++K+ LK GD Sbjct: 77 EWVGTRYRMGGTTK--RGIDCSAFMQTTFSEVFGIELPRSTAEQRHLGRKINKSELKKGD 134 Query: 112 LVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGNWKRTYLKARRVL 168 LVFF R N HV VYIGN + +H GVTIS L+ W RTY ++RR++ Sbjct: 135 LVFF----RKNN----HVGVYIGNNQFMHASTGQGVTISSLDEKYWARTYTQSRRIM 183 >BS_ywtD [M] COG0791 Cell wall-associated hydrolases (invasion-associated proteins) Length = 413 Score = 87.4 bits (215), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 47/124 (37%), Positives = 75/124 (60%), Gaps = 3/124 (2%) Query: 45 QQIVNLGKKYMGVRYEFGAPTSSTRTFDCSSFTKYIFGKYGVNLPRTSSAQSKVGKSVSK 104 + IV+ K +G +Y++G T FD S +Y+F K ++LPR+ + Q K+G +V Sbjct: 37 ELIVSEAKNLLGYQYKYGGETPK-EGFDPSGLIQYVFSKADIHLPRSVNDQYKIGTAVKP 95 Query: 105 ANLKVGDLVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDL-NSGNWKRTYLK 163 NLK GD++FF + G+ TH A+YIG+G+++H+ S GV I++ S W TY+ Sbjct: 96 ENLKPGDILFFKK-EGSTGTVPTHDALYIGDGQMVHSTQSKGVIITNYKKSSYWSGTYIG 154 Query: 164 ARRV 167 ARR+ Sbjct: 155 ARRI 158 Score = 81.3 bits (199), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 45/121 (37%), Positives = 68/121 (56%), Gaps = 5/121 (4%) Query: 47 IVNLGKKYMGVRYEFGAPTSSTRTFDCSSFTKYIFGK-YGVNLPRTSSAQSKVGKSVSKA 105 +V +KY+GV Y FG T S FDCS +Y+F + G+ LPR++ Q VG+ V+ Sbjct: 169 VVQEAEKYIGVPYVFGGSTPS-EGFDCSGLVQYVFQQALGIYLPRSAEQQWAVGEKVAPQ 227 Query: 106 NLKVGDLVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGNWKRTYLKAR 165 N+K GD+V+FS+ + + ++H +Y G G+ + S VTIS L+ WK R Sbjct: 228 NIKPGDVVYFSNTYK---TGISHAGIYAGAGRFIQASRSEKVTISYLSEDYWKSKMTGIR 284 Query: 166 R 166 R Sbjct: 285 R 285 Score = 75.5 bits (184), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 48/124 (38%), Positives = 74/124 (59%), Gaps = 11/124 (8%) Query: 47 IVNLGKKYMG-VRYEFGAPTSSTRTFDCSSFTKYIFGKY-GVNLPRTSSAQSKVGKSVSK 104 IV+ Y+G V Y+ G T T FD + F +Y++ K G++LPR +++Q G + K Sbjct: 297 IVSEATLYVGEVPYKQGGVTPET-GFDTAGFVQYVYQKAAGISLPRYATSQYNAGTKIEK 355 Query: 105 ANLKVGDLVFFSSGSRANGSNVTHVAVYIGNGKILHTYGSPGVTISDLNSGN-WKRTYLK 163 A+LK GD+VFF S S + ++YIGNG+++H S GVTI+++N+ WK Y Sbjct: 356 ADLKPGDIVFFQSTS-------LNPSIYIGNGQVVHVTLSNGVTITNMNTSTYWKDKYAG 408 Query: 164 ARRV 167 + RV Sbjct: 409 SIRV 412 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,612,374 Number of extensions: 352452 Number of successful extensions: 698 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 618 Number of HSP's successfully gapped: 61 Length of query: 168 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 69 Effective length of database: 26,960,365 Effective search space: 1860265185 Effective search space used: 1860265185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 112 (47.8 bits)