BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0041.p (356 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_moeB [H] COG0476 Dinucleotide-utilizing enzymes involved in m... 376 e-104 BS_yjbU [H] COG0476 Dinucleotide-utilizing enzymes involved in m... 369 e-102 PA2103_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in ... 135 1e-31 AF0553 [H] COG0476 Dinucleotide-utilizing enzymes involved in mo... 131 1e-30 PA4663 [H] COG0476 Dinucleotide-utilizing enzymes involved in mo... 130 3e-30 >BS_moeB [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 339 Score = 376 bits (965), Expect = e-104, Method: Compositional matrix adjust. Identities = 186/336 (55%), Positives = 238/336 (70%), Gaps = 2/336 (0%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ+RF G EGQ LA S VLI+G GALGT AE L+R G G I + DRDYVEWSNL Sbjct: 5 YSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNL 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQQLYTE DA+ RMPKA AAK L +INSEI IEA V + AE LE L+ D+++D T Sbjct: 65 QRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDAT 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 DNF+TR++IND+AQK PW+YGACV S G+ T++PGETPCL+CL +P+GG TCDTA Sbjct: 125 DNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVIPGETPCLSCLFEQIPVGGATCDTA 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 GI+P AV +V+A AEALKLL G+ E ++ ++FDVW N H +I V +++ CPSCG Sbjct: 185 GIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREECPSCG 244 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARLAGLGSGKVESNPYLVS 320 ++ VYPYL NT ++ VLCGRDTVQ+R +++ QE RL + GKVE+N +L+ Sbjct: 245 ANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTI--GKVEANAFLLH 302 Query: 321 FTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 E +R+V+F DGRAL+HGT+D+ A S R G Sbjct: 303 IFYEDFRIVIFNDGRALVHGTNDVKEANSVLARVIG 338 >BS_yjbU [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 211 Score = 369 bits (946), Expect = e-102, Method: Compositional matrix adjust. Identities = 0/338 (0%), Positives = 0/338 (0%), Gaps = 8/338 (2%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 ++ + + A+ + + Sbjct: 5 KRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAM 64 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 + + + + + + + + LPGV Sbjct: 65 LELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANV 124 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGDTCDTA 200 H + + Sbjct: 125 VLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGR 184 Query: 201 GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPSCG 260 + + + Sbjct: 185 YTCIARKPRCGSCIIEDLCEYKEK-- 242 Query: 261 SHPVYPYLTAANTERSDVLCGRDTVQIRPAQRRQLNLQETAARL--AGLGSGKVESNPYL 318 Sbjct: 243 ---- 298 Query: 319 VSFTEEPYRMVVFGDGRALIHGTSDIAAARSFYHRYFG 356 Sbjct: 299 336 >PA2103_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 266 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 89/245 (36%), Positives = 123/245 (50%), Gaps = 12/245 (4%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 Y R + G EGQ L Q+ VLIIG G LG+ LA G G I L D D VE SNL Sbjct: 21 YVRHITLPGVGREGQQRLKQARVLIIGTGGLGSPACLYLAAAGVGTIGLVDFDVVESSNL 80 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQ ++ + MPK +A+ R++ +N I + + A+ +SL+ DL++DGT Sbjct: 81 QRQVVHGY--STLGMPKVESAQRRMQDLNPYIEVVTHRYAIDADNAQSLIQSYDLVLDGT 138 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLL--GAVPLGGDTCD 198 DNF TR ++N + K G P ++GA G PC CL P +C+ Sbjct: 139 DNFATRYLVNAVCAKLGKPLVFGAIYRFEGQVSVFNLEGGPCYQCLFPNNPPPEMAPSCN 198 Query: 199 TA---GILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDS 255 G+LP + L+ A EA+K L G + L G+LL FD E VK ++D Sbjct: 199 AGGVIGVLPGVIGLLQAT---EAVKALVGLGKSLSGRLLRFDALLMHFNE--VKFNRRDD 253 Query: 256 CPSCG 260 CP+CG Sbjct: 254 CPACG 258 >AF0553 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 267 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 77/242 (31%), Positives = 121/242 (50%), Gaps = 7/242 (2%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 Y RQ+ G GQ L ++ VL++G G LG+ E LA G GRI +AD D V+ +NL Sbjct: 10 YGRQILIPEIGGRGQEKLLKAKVLVVGAGGLGSPAIEFLAAAGVGRIGIADGDEVDITNL 69 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 RQ ++ + KA +A + +E +N ++ ++ + AE ++ D+++D T Sbjct: 70 HRQTIHA---GNLGVNKAESAASFVEKLNPDVEVDVYPFHLSAENAREVISKYDVVLDCT 126 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYGITFTILPGETPCLNCLLGAVPLGGD--TCD 198 D+F +R +IND P+++ A + G T++P ET C C L P G +C Sbjct: 127 DSFSSRFLINDACVLESKPFVHAAVLRFEGQIMTVIPHETACYRCFLPEAPPPGSVPSCR 186 Query: 199 TAGILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPS 258 AGI+ EA+KL+ G E +LL D+ N+ I VK K++ C Sbjct: 187 EAGIIGTTTAFFGVLQANEAIKLILGTGELFTNRLLYADILHNDFNFIEVK--KQEKCRV 244 Query: 259 CG 260 CG Sbjct: 245 CG 246 >PA4663 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 252 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 87/242 (35%), Positives = 127/242 (52%), Gaps = 6/242 (2%) Query: 21 YSRQVRFTPFGAEGQAGLAQSTVLIIGVGALGTGIAETLARCGTGRIILADRDYVEWSNL 80 YSRQ+ EGQ L +S LI+G+G LG+ +A LA G G + LAD D V+ +NL Sbjct: 10 YSRQILLAQVDVEGQLRLKRSRALIVGLGGLGSPVALYLAAAGVGELHLADFDTVDLTNL 69 Query: 81 QRQQLYTEEDARQRMPKAAAAKARLESINSEITIEARVLDVRAEELESLLPGVDLIMDGT 140 QRQ ++ + A + K +A RL ++N +I + + + + L++ + VDL++D T Sbjct: 70 QRQVIH--DGASVGIGKVESAMRRLGALNPQIRLVSHSAALDEDSLDAAVAAVDLVLDCT 127 Query: 141 DNFDTRLIINDMAQKHGIPWIYGACVGSYG--ITFTILPGETPCLNCLLGAVPLGGDTCD 198 DNF TR +N + G P + GA + G F E+PC +CL G TC Sbjct: 128 DNFATREAVNVACVRAGKPLVSGAAIRLEGQLSVFDTRRDESPCYHCLYGHGSEAELTCS 187 Query: 199 TAGILPQAVQLVTANATAEALKLLGGRPEQLRGKLLSFDVWRNEHQEIGVKAAKKDSCPS 258 AG++ V LV + EALKLL G E L G+LL D +E+ V+ + SC Sbjct: 188 EAGVIGPLVGLVGSLQALEALKLLAGFGEPLVGRLLLVDALGTRFRELRVR--RDPSCAV 245 Query: 259 CG 260 CG Sbjct: 246 CG 247 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,634,421 Number of extensions: 1000359 Number of successful extensions: 2661 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 2578 Number of HSP's successfully gapped: 54 Length of query: 356 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 249 Effective length of database: 26,037,693 Effective search space: 6483385557 Effective search space used: 6483385557 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)