BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0029.p (316 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjK [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 285 5e-77 lin2084 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 285 9e-77 BH1713 [R] COG1234 Metal-dependent hydrolases of the beta-lactam... 258 9e-69 SPy0924 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 256 5e-68 STM2313 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 248 9e-66 >BS_yqjK [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Length = 252 Score = 285 bits (730), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 0/307 (0%), Positives = 0/307 (0%), Gaps = 1/307 (0%) Query: 1 MEIYFLGTNAGVPTLQRNVTSVALRLLEERRSFWMFDCGEGTQHQVLRSPLRLGKLEKLF 60 M + + L + ++ D G G + R Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 ITHLHGDHLFGLPGLLSSRGYQGGTGPLTVYGPPGLKAYLDISLSVSQSRIPYPLEIVEH 120 I L + + L + +P + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 121 SGGLIFEDDGFKVEAGLLEHRIDSYGYRVTEKDSPGNLNKELLLSFXXXXXXXXXXXXXX 180 + + + + D + + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 181 QDVTTEEGVLIRAAEVVNAPKRGRIVTVLGDTRPCQGTLPLALDADLIIHEATFGHDLKE 240 + + V A + L ++ + G L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 241 MAHQYHHSTARQAAELARDAGGRELLLTHFSSRYTTXXXXXXXXXXXXXIFPQTLLAEEF 300 Sbjct: 241 - 299 Query: 301 CTFPVYR 307 Sbjct: 300 306 >lin2084 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Length = 252 Score = 285 bits (728), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 0/305 (0%), Positives = 0/305 (0%), Gaps = 1/305 (0%) Query: 1 MEIYFLGTNAGVPTLQRNVTSVALRLLEERRSFWMFDCGEGTQHQVLRSPLRLGKLEKLF 60 M + + L + ++ D G G + R Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 ITHLHGDHLFGLPGLLSSRGYQGGTGPLTVYGPPGLKAYLDISLSVSQSRIPYPLEIVEH 120 I L + + L + +P + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 121 SGGLIFEDDGFKVEAGLLEHRIDSYGYRVTEKDSPGNLNKELLLSFXXXXXXXXXXXXXX 180 + + + + D + + Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 181 QDVTTEEGVLIRAAEVVNAPKRGRIVTVLGDTRPCQGTLPLALDADLIIHEATFGHDLKE 240 + + V A + L ++ + G L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 241 MAHQYHHSTARQAAELARDAGGRELLLTHFSSRYTTXXXXXXXXXXXXXIFPQTLLAEEF 300 Sbjct: 241 - 299 Query: 301 CTFPV 305 Sbjct: 300 304 >BH1713 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Length = 252 Score = 258 bits (659), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 0/304 (0%), Positives = 0/304 (0%), Gaps = 3/304 (0%) Query: 1 MEIYFLGTNAGVPTLQRNVTSVALRLLEERRSFWMFDCGEGTQHQVLRSPLRLGKLEKLF 60 M + + L + ++ D G G + R Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 ITHLHGDHLFGLPGLLSSRGYQGGTGPLTVYGPPGLKAYLDISLSVSQSRIPYPLEIVEH 120 I L + + L + +P + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 121 SG-GLIFEDDGFKVEAGLLEHRIDSYGYRVTEKDSPGNLNKELLLSFXXXXXXXXXXXXX 179 + G++ + + L Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 180 XQDVTTEEGVLIRAAEVVNAPKRGRIVTVLGDTRPCQGTLPLALDADLIIHEATFGHDLK 239 V R + + + + + + L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 240 EMAHQYHHSTARQAAELARDAGGRELLLTHFSSRYTTXXXXXXXXXXXXXIFPQTLLAEE 299 Sbjct: 241 -- 298 Query: 300 FCTF 303 Sbjct: 299 302 >SPy0924 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Length = 252 Score = 256 bits (653), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 0/279 (0%), Positives = 0/279 (0%), Gaps = 5/279 (1%) Query: 1 MEIYFLGTNAGVPTLQRNVTSVALRLLEERRSFWMFDCGEGTQHQVLRSPLRLGKLEKLF 60 M + + L + ++ D G G + R Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 ITHLHGDHLFGLPGLLSSRGYQGG--TGPLTVYGPPGLKAYLDISLSVSQSRIPYPL--- 115 I L + + L P+ + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 116 EIVEHSGGLIFEDDGFKVEAGLLEHRIDSYGYRVTEKDSPGNLNKELLLSFXXXXXXXXX 175 + ++ + E + + G +LS Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 176 XXXXXQDVTTEEGVLIRAAEVVNAPKRGRIVTVLGDTRPCQGTLPLALDADLIIHEATFG 235 Q + + + A + + + + + Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 236 HDLKEMAHQYHHSTARQAAELARDAGGRELLLTHFSSRY 274 Sbjct: 241 279 >STM2313 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Length = 252 Score = 248 bits (633), Expect = 9e-66, Method: Compositional matrix adjust. Identities = 0/306 (0%), Positives = 0/306 (0%), Gaps = 2/306 (0%) Query: 1 MEIYFLGTNAGVPTLQRNVTSVALRLLEERR-SFWMFDCGEGTQHQVLRSPLRLGKLEKL 59 M + + L + + L Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 60 FITHLHGDHLFGLPGLLSSRGYQGGTGPLTVYGPPGLKAYLDISLSVSQSRIPYPLEIVE 119 + V + A + + L + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 120 HSGGLIFEDDGFKVEAGLLEHRIDSYGYRVTEKDSPGNLNKELLLSFXXXXXXXXXXXXX 179 + G++ + + L Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 180 XQDVTTEEGVLIRAAEVVNAPKRGRIVTVLGDTRPCQGTLPLALDADLIIHEATFGHDLK 239 V R + + + + + + L Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 240 EMAHQYHHSTARQAAELARDAGGRELLLTHFSSRYTTXXXXXXXXXXXXXIFPQTLLAEE 299 Sbjct: 241 - 299 Query: 300 FCTFPV 305 Sbjct: 300 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,737,782 Number of extensions: 855300 Number of successful extensions: 1604 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 1573 Number of HSP's successfully gapped: 28 Length of query: 316 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 210 Effective length of database: 26,153,027 Effective search space: 5492135670 Effective search space used: 5492135670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)