BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0028.p (1145 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc03112_2 [E] COG1410 Methionine synthase I, cobalamin-binding ... 445 e-124 BH1630_1 [E] COG0646 Methionine synthase I (cobalamin-dependent)... 423 e-118 SMc03112_1 [E] COG0646 Methionine synthase I (cobalamin-dependen... 272 2e-72 CAC0578_1 [E] COG0646 Methionine synthase I (cobalamin-dependent... 261 7e-69 BH1629_1 [E] COG0646 Methionine synthase I (cobalamin-dependent)... 162 3e-39 >SMc03112_2 [E] COG1410 Methionine synthase I, cobalamin-binding domain Length = 913 Score = 445 bits (1145), Expect = e-124, Method: Compositional matrix adjust. Identities = 308/870 (35%), Positives = 447/870 (51%), Gaps = 55/870 (6%) Query: 321 LSGIEPVYIESDNRPYM-VGERTNVLGSRKFKRLIVEGKYEEASEIARAQVKNGAQVIDV 379 LSG+EP + +D P++ VGERTNV GS KF++LI G Y A ++AR QV NGAQ+IDV Sbjct: 13 LSGLEPFTL-TDEIPFVNVGERTNVTGSAKFRKLITAGDYAAALDVARDQVANGAQIIDV 71 Query: 380 CVQDPDRDESADITKFLELXXXX---XXXPLMIDTTDPKVIDLALQYSQGKAIINSINLE 436 + + D + +FL L P+MID++ +VI+ L+ QGKA++NSI+L+ Sbjct: 72 NMDEGLIDSKQVMVEFLNLVASEPDIARVPVMIDSSKWEVIEAGLKCVQGKALVNSISLK 131 Query: 437 DGEEKFELVTPLIHKYGAAIVVGTIDESGQAIKAADKLNVAKRSYELLVNKYGLAPEDLI 496 +GE F L+ YGAA+VV DE GQA K+ + +R+Y LL + G PED+I Sbjct: 132 EGEAAFLHHARLVRAYGAAVVVMAFDEKGQADTKTRKVEICRRAYRLLTEEVGFPPEDII 191 Query: 497 FDTLVFPVGTGDEQYIGSAKETIDGIRLIKEALPEVHTILGISNVSFGLP--EAGREVLN 554 FD +F V TG E++ + I+ I ALP VH G+SN+SF E RE ++ Sbjct: 192 FDPNIFAVATGIEEHNNYGVDFIEATHEIIAALPHVHVSGGVSNLSFSFRGNEPVREAMH 251 Query: 555 SVFLYECTKAGLDYAIVNTEKVERYASIPEQERYLAEQLIYNTNDDT---LSAFVAAFRG 611 ++FLY +AG+D IVN ++ Y +I + R E ++ N D+ L +RG Sbjct: 252 AIFLYHAIQAGMDMGIVNAGQLAVYDAIDPELRETCEDVVLNRRADSTERLLEIAERYRG 311 Query: 612 KK----VEKKEKISNLSLEERLASYVVEGTKEGLIPDLNEA-LTKSGPLEIINGPLMAGM 666 K EK +E+RL +V G E + D EA L PL +I GPLMAGM Sbjct: 312 KGGSQGKEKDLAWREWPVEKRLEHALVNGITEFIEADTEEARLAAERPLHVIEGPLMAGM 371 Query: 667 SEVGRLFNNNELIVAEVLQSAEVMKASVSHLEQFMQKDETS--------VKGKIMLATVK 718 + VG LF + ++ + +V++SA VMK +V+ L M++++ + GKI++ATVK Sbjct: 372 NVVGDLFGSGKMFLPQVVKSARVMKQAVAVLLPHMEEEKRANGGGEARESAGKILMATVK 431 Query: 719 GDVHDIGKNLVEIILSNNGYEIINLGIKVPPESIIEAFRREKADAIGLSGLLVKSAQQMV 778 GDVHDIGKN+V ++L+ N YEII+LG+ VP I+E R +K D +GLSGL+ S +M Sbjct: 432 GDVHDIGKNIVGVVLACNNYEIIDLGVMVPSAKILEVAREQKVDIVGLSGLITPSLDEMA 491 Query: 779 LTAQDLRTAGIDVPIMVGGAALTRKFTKTRIRPEYD-GLVLYAKDAMDGLDIANRLMNPE 837 A +L G DVP+++GGA +R T +I P Y G +Y DA + + + L++PE Sbjct: 492 HVASELEREGFDVPLLIGGATTSRVHTAVKINPRYSLGQTVYVTDASRAVGVVSSLLSPE 551 Query: 838 ER------VKIXXXXXXXXXXXXXXXXXXXXXXLTRAVRSKISPDA-PVFTPPDLERHVL 890 R V+ RA +I DA P L V Sbjct: 552 VRDSYKKTVRAEYLKVADAHARNEAEKRRLPLSQARANAFRIDWDAHQPKVPSFLGTRVF 611 Query: 891 RNYPLGHIIPYVNMQMLLGHHLGLKGSV------EAQLAAGDPRTVELKETVDEILHHAM 944 + L + Y++ LKG E Q AA +L E ++ + Sbjct: 612 EGWDLAELARYIDWTPFF-QTWELKGVFPKILDDERQGAAAR----QLFEDAQAMVEKIV 666 Query: 945 LEGTMTPHAMYQFFPAQSKGNDILIYDPQDTATVLHTFTFPRQNV-----EPYLCLADFL 999 E P A+ F+PA S G+D+ ++ + L TF RQ + P + LADF+ Sbjct: 667 AEAWFAPKAVIGFWPAASMGDDVRLFADEVREAELATFFTLRQQMVKRDGRPNVALADFV 726 Query: 1000 KSVDSGIMDYVGFLVVTAGHGIREMS-AEL--RDKGDYLRSHXXXXXXXXXXXXXXXXXH 1056 SG DYVG VVTA GI E++ AE R DY Sbjct: 727 APAASGKRDYVGGFVVTA--GIEEVAIAERFERANDDYSSIMVKALADRFAEAFAERMHE 784 Query: 1057 HMMRDTWGF-PDPADMTMKQRHGARYQGIRVSFGYPACPDLEDQGPLFKLM-SPGDIGVE 1114 ++ ++ WG+ PD A T ++ Y GIR + GYPA PD ++ LF+L+ + IGV Sbjct: 785 YVRKELWGYAPDEA-FTPQELIAEPYAGIRPAPGYPAQPDHTEKETLFRLLDAEAAIGVR 843 Query: 1115 LTEGFMMEPEASVSAMVFAHPEAQYFNVEK 1144 LTE + M P +SVS + HP++ YF V K Sbjct: 844 LTESYAMWPGSSVSGLYVGHPDSYYFGVAK 873 >BH1630_1 [E] COG0646 Methionine synthase I (cobalamin-dependent), methyltransferase domain Length = 305 Score = 423 bits (1088), Expect = e-118, Method: Compositional matrix adjust. Identities = 193/305 (63%), Positives = 244/305 (80%) Query: 1 VAKFTLAESLQQRILILDGAMGTMIQQVPLTGADFGGEELDGCNEMLVLTRPELIQGIHE 60 + K + L+++I+ILDGAMGTM+Q LT DFGGEE +GCNE L T P +++ IH Sbjct: 1 MTKSLFEQQLERKIVILDGAMGTMLQAANLTADDFGGEEYEGCNEYLNETAPHVVEDIHR 60 Query: 61 KYLEAGADLIETNTFGATSVVLAEYDIPQRAREINLEAARLARNAVDKYDTPDRPRYVIG 120 YLEAGAD+I TNTFGAT +VL +YD+ +A E+N+ A ++A+ +++ TPD PR+V G Sbjct: 61 AYLEAGADVIATNTFGATDIVLDDYDLGYKAEELNICAVKIAKRVAEEFSTPDWPRFVAG 120 Query: 121 AMGPTTKTLSVTGGVTFQELVQSYEEQAAALIDGKVDALLLETSQDTLNVKAGSIGIRNA 180 AMGPTTK+LSVTGG TF++L++SY +QA LI G D LLLETSQD NVKA +G+ A Sbjct: 121 AMGPTTKSLSVTGGATFEQLIESYRQQATGLIKGGADILLLETSQDMRNVKAAYLGLSQA 180 Query: 181 FERSGITLPVMISGTIEPMGTTLAGQNIEAFYISLEHLKPISIGLNCATGPEFMRDHIRS 240 + + LP++ISGTIEPMGTTLAGQNIEAFY+SLEH+ P+ +GLNCATGPEFMRDH+RS Sbjct: 181 QKELEVKLPLIISGTIEPMGTTLAGQNIEAFYLSLEHMNPVVVGLNCATGPEFMRDHLRS 240 Query: 241 LSAISSAAVSCYPNAGLPDENGNYHESPDSLARKLAGFAEKGWLNIAGGCCGTTPDHIRA 300 LS +++ +VSCYPNAGLPDE GNYHESP+SLA KLAGFAEKGWLN+ GGCCGTTPDHIRA Sbjct: 241 LSDLATCSVSCYPNAGLPDEEGNYHESPESLAAKLAGFAEKGWLNMVGGCCGTTPDHIRA 300 Query: 301 MAEVL 305 + +V+ Sbjct: 301 LLDVM 305 >SMc03112_1 [E] COG0646 Methionine synthase I (cobalamin-dependent), methyltransferase domain Length = 344 Score = 272 bits (696), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 151/321 (47%), Positives = 201/321 (62%), Gaps = 17/321 (5%) Query: 6 LAESLQQRILILDGAMGTMIQQVPLTGADFGGEELDGC-------NEMLVLTRPELIQGI 58 LA++ +RILI+DGAMGT IQQ+ F GE GC N++L LT+P+ I+ I Sbjct: 24 LAQAAAERILIMDGAMGTEIQQLGFVEDHFRGERFGGCACHQQGNNDLLTLTQPKAIEDI 83 Query: 59 HEKYLEAGADLIETNTFGATSVVLAEYDIPQRAREINLEAARLARNAVDKYDTPD-RPRY 117 H Y AGAD++ETNTF +T + A+Y + ++N + ARLAR A + + D R R+ Sbjct: 84 HYHYAIAGADILETNTFSSTRIAQADYGMEDMVYDLNRDGARLARRAAKRAEAEDGRRRF 143 Query: 118 VIGAMGPTTKTLSVT--------GGVTFQELVQSYEEQAAALIDGKVDALLLETSQDTLN 169 V GA+GPT +T S++ V+F +L +Y EQ LIDG D +L+ET DTLN Sbjct: 144 VAGALGPTNRTASISPDVNNPGYRAVSFDDLRLAYAEQVRGLIDGGADIILIETIFDTLN 203 Query: 170 VKAGSIGIRNAFERSGITLPVMISGTIEPM-GTTLAGQNIEAFYISLEHLKPISIGLNCA 228 KA + F G+ LPVMISGTI + G TL+GQ AF+ S+ H P +IGLNCA Sbjct: 204 AKAAIFATQEVFAEKGVRLPVMISGTITDLSGRTLSGQTPTAFWYSVRHADPFTIGLNCA 263 Query: 229 TGPEFMRDHIRSLSAISSAAVSCYPNAGLPDENGNYHESPDSLARKLAGFAEKGWLNIAG 288 G MR HI LSA++ V YPNAGLP+E G Y ESP+ +A ++ GFA G +NI G Sbjct: 264 LGANAMRAHIDELSAVADTLVCAYPNAGLPNEFGRYDESPEQMAAQVEGFARDGLVNIVG 323 Query: 289 GCCGTTPDHIRAMAEVLAEYP 309 GCCG+TP HIRA+AE +A+YP Sbjct: 324 GCCGSTPAHIRAIAEAVAKYP 344 >CAC0578_1 [E] COG0646 Methionine synthase I (cobalamin-dependent), methyltransferase domain Length = 322 Score = 261 bits (666), Expect = 7e-69, Method: Compositional matrix adjust. Identities = 141/319 (44%), Positives = 196/319 (61%), Gaps = 18/319 (5%) Query: 5 TLAESLQQRILILDGAMGTMIQQVPLTGADFGGEELDGC-------NEMLVLTRPELIQG 57 +L L +IL+LDGAMGT IQ L DF G C N++L LT+PE+I+ Sbjct: 4 SLKNLLNNKILVLDGAMGTCIQSFNLDEGDFKGSLSCTCHSNQKGNNDVLNLTKPEIIKE 63 Query: 58 IHEKYLEAGADLIETNTFGATSVVLAEYDIPQRAREINLEAARLARNAVDKYD--TPDRP 115 IH++YLEAGAD+IETNTF AT + +Y++ + +IN + A+LA+ A Y P++P Sbjct: 64 IHKRYLEAGADIIETNTFNATEISQKDYNMQDKIYDINFKGAKLAKEACTYYTKLNPNKP 123 Query: 116 RYVIGAMGPTTKTLSVT--------GGVTFQELVQSYEEQAAALIDGKVDALLLETSQDT 167 R+ G++GPT +T S++ VTF EL +Y+ Q ALIDG VD LL+ET DT Sbjct: 124 RFAAGSIGPTNRTASLSPDVENPGFRNVTFDELCNAYKHQIEALIDGGVDLLLIETIFDT 183 Query: 168 LNVKAGSIGIRNAFERSGITLPVMISGTI-EPMGTTLAGQNIEAFYISLEHLKPISIGLN 226 LN +A FE I LP++ISGTI + G L+GQ ++AF SL++ I+IGLN Sbjct: 184 LNARAAIFAAETVFENKKIKLPIIISGTIADKSGRILSGQTLDAFAESLKNENIIAIGLN 243 Query: 227 CATGPEFMRDHIRSLSAISSAAVSCYPNAGLPDENGNYHESPDSLARKLAGFAEKGWLNI 286 C+ G E + I+ LS + +S +PNAGLP+ G Y E P+ A + A +G LNI Sbjct: 244 CSFGAEELIPFIKRLSETQNRYISFHPNAGLPNSLGEYEELPEETASIVKKLALEGHLNI 303 Query: 287 AGGCCGTTPDHIRAMAEVL 305 GGCCGTTP+HIRA++ V+ Sbjct: 304 VGGCCGTTPEHIRAISSVV 322 >BH1629_1 [E] COG0646 Methionine synthase I (cobalamin-dependent), methyltransferase domain Length = 283 Score = 162 bits (410), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 108/304 (35%), Positives = 157/304 (51%), Gaps = 27/304 (8%) Query: 5 TLAESLQQRILILDGAMGTMIQQVPLTGADFGGEELDGCNEMLVLTRPELIQGIHEKYLE 64 L E+L+ IL+ DGAMGT++ + + +D C E L +T PE I H Y+E Sbjct: 3 NLVEALKTNILVGDGAMGTLLYE----------QGIDRCFEELNVTDPEKIVAAHVAYVE 52 Query: 65 AGADLIETNTFGATSVVLAEYDIPQRAREINLEAARLARNAVDKYDTPDRPRYVIGAMGP 124 AGAD+I+TNT+ A + LA+Y + + EIN A RLAR A + +V+G +G Sbjct: 53 AGADVIQTNTYAANRMKLAKYQLDDQVLEINRAAVRLARKAAKQ------ETFVLGTIGG 106 Query: 125 TTKTLSVTGGVTFQELVQSYEEQAAALIDGKVDALLLETSQDTLNVKAGSIGIRNAFERS 184 V QE+ + EQ AL+ VD LLLET D K + + RS Sbjct: 107 IRSVQFEE--VEIQEVQDVFLEQMKALVSEGVDGLLLETFYDLEEAK-----LAVSLARS 159 Query: 185 GITLPVMISGTIEPMGTTLAGQNIEAFYISLEHLKPISIGLNCATGPEFMRDHIRSLSAI 244 LPV+ +I +G G+ +E + LE L +G+NC GP M + ++ + Sbjct: 160 LTDLPVIAHLSIAEIGVLQGGKLLEEAFAELEGLGADLVGINCRMGPYQMLRSLETVQLL 219 Query: 245 SSAAVSCYPNAGLPD-ENGN--YHESPDSLARKLAGFAEKGWLNIAGGCCGTTPDHIRAM 301 A S YPNA LPD +G YH +P+ F ++G + + GGCCGTTP+H+RA Sbjct: 220 DRAYYSAYPNASLPDYRDGRLYYHSNPEYFYEMGKRFVQQG-VRLLGGCCGTTPEHVRAF 278 Query: 302 AEVL 305 A+V+ Sbjct: 279 AKVV 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 82,416,405 Number of extensions: 3552013 Number of successful extensions: 9274 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 9235 Number of HSP's successfully gapped: 24 Length of query: 1145 Length of database: 38,378,431 Length adjustment: 117 Effective length of query: 1028 Effective length of database: 24,884,353 Effective search space: 25581114884 Effective search space used: 25581114884 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 121 (51.2 bits)