BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0026.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2155 [R] COG1011 Predicted hydrolase (HAD superfamily) 272 4e-73 BS_ysaA [R] COG1011 Predicted hydrolase (HAD superfamily) 260 1e-69 YPO2295 [R] COG1011 Predicted hydrolase (HAD superfamily) 94 3e-19 SA1018 [R] COG1011 Predicted hydrolase (HAD superfamily) 86 6e-17 STM4559 [R] COG1011 Predicted hydrolase (HAD superfamily) 83 5e-16 >BH2155 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 267 Score = 272 bits (695), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 134/261 (51%), Positives = 173/261 (66%), Gaps = 4/261 (1%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGD--SVDPXXXXXXXXXXXXNLYESYETFP 60 + A++FDLDDTLLWD++SV+EAF + A S+DP LY SYET+ Sbjct: 2 VKAIIFDLDDTLLWDKKSVKEAFVATCQYAATKRSLDPEALEESVREVARELYASYETYE 61 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 FT++IGINPFE LWG F FR+M+++ P YRK++W +GLA LG+ED Sbjct: 62 FTQMIGINPFEGLWGQFLDDND-HFRKMKEIVPTYRKDAWTQGLAKLGIEDQALGEELAE 120 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 R+ P++YEET +VLD+LKG +LLLLTNG P LQQ KLD PELVPYFDH+VIS Sbjct: 121 RFPEERKKHPFVYEETFRVLDQLKGNYQLLLLTNGSPHLQQTKLDITPELVPYFDHIVIS 180 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FG+GKPD IF HA++LL +T +E IMVGD L TDILG G+ +VWINR K Sbjct: 181 GGFGRGKPDPSIFEHALSLLSLTKDEAIMVGDNLMTDILGASRVGIKSVWINREQKEQG- 239 Query: 241 EITPDYQIGHLSELLPLVETL 261 E+ P Y+I HL +LL +++ L Sbjct: 240 EVEPTYEIEHLEQLLDILKKL 260 >BS_ysaA [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 260 Score = 260 bits (665), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 128/259 (49%), Positives = 166/259 (64%), Gaps = 2/259 (0%) Query: 3 ISAVLFDLDDTLLWDERSVEEAFRYASEAAGDS--VDPXXXXXXXXXXXXNLYESYETFP 60 + AV FDLDDTLLWDE+SV F A + P LY SYET+P Sbjct: 1 MKAVFFDLDDTLLWDEKSVRTTFAETCLQAEKKYGLAPEEFEAAVREAARELYMSYETYP 60 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKESWRRGLAALGVEDXXXXXXXXX 120 +T +IGINPFE LW NF+ F+++ ++ P YR+ +W GL ALG++D Sbjct: 61 YTVMIGINPFEGLWSNFSEPISEGFQKLNKIVPGYRRNAWTNGLKALGIDDPAYGEYLGE 120 Query: 121 XXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVIS 180 RR RP++Y+ET VLD+LKGK +LLLLTNG P+LQ+EKL GVPEL PYF+ +VIS Sbjct: 121 FFAAERRKRPFVYDETFAVLDQLKGKYELLLLTNGDPSLQKEKLAGVPELAPYFNEIVIS 180 Query: 181 GNFGKGKPDKGIFTHAMNLLDITPEEGIMVGDKLTTDILGGLGAGLTTVWINRTGKASDP 240 G FGKGKPD IF H + L++I ++ IMVGD L TDILG AG+ TVWINRT K ++ Sbjct: 181 GAFGKGKPDVSIFEHCLKLMNIEKDDAIMVGDNLNTDILGASRAGIKTVWINRTDKKNET 240 Query: 241 EITPDYQIGHLSELLPLVE 259 ++ PDY I L +L P++E Sbjct: 241 DVKPDYIISSLHDLFPILE 259 >YPO2295 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 224 Score = 93.6 bits (231), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 52/124 (41%), Positives = 75/124 (60%), Gaps = 6/124 (4%) Query: 138 QVLDELKGKVKLLLLTNGCPALQQEKLD--GVPELVPYFDHVVISGNFGKGKPDKGIFTH 195 +++D L GKV + ++TNG LQ +L+ G ++ F ++IS G KPD IF + Sbjct: 102 ELVDALSGKVNMGIITNGFTELQTIRLERTGFKDI---FSPLIISEQVGVAKPDVAIFEY 158 Query: 196 AMNLLDITPEEGI-MVGDKLTTDILGGLGAGLTTVWINRTGKASDPEITPDYQIGHLSEL 254 A N++D P+E I MVGD L +DI GG+ AG+ T W+N G A D I P YQ+ L+EL Sbjct: 159 AFNVMDNPPKEHILMVGDNLHSDIQGGINAGIDTCWLNTQGVAGDDNIAPRYQVSSLAEL 218 Query: 255 LPLV 258 L+ Sbjct: 219 QALL 222 >SA1018 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 228 Score = 85.9 bits (211), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 71/262 (27%), Positives = 109/262 (41%), Gaps = 39/262 (14%) Query: 1 MPISAVLFDLDDTLLWDERSVEEAFRYASEAAGDSVDPXXXXXXXXXXXXNLYESYETFP 60 M +L D DDT++ + E AF Y + ++ +E F Sbjct: 1 MGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDFLTFKKIN----HQHWEAFQ 56 Query: 61 FTKLIGINPFEALWGNFTAGEQLEFRQMEQLAPVYRKES-WRRGLAALGVEDXXXXXXXX 119 KL + N+ Q+E +R + +R GLA V+ Sbjct: 57 QNKLTKSEVLSERFVNYFKHHQME-------VDGHRADVLFRNGLAEAKVK--------- 100 Query: 120 XXXXXXRRSRPYIYEETLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVI 179 +++TL+ + EL + L ++TNG Q+ +L+ P L Y + I Sbjct: 101 ------------YFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTP-LHKYIKKIFI 147 Query: 180 SGNFGKGKPDKGIFTHAMNLLDITPEE---GIMVGDKLTTDILGGLGAGLTTVWINRTGK 236 S G KP+ F + N DI +E I+VGD LT+DILGG+ AG+ T W N G Sbjct: 148 SEETGYQKPNPEFFNYVFN--DIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGF 205 Query: 237 ASDPEITPDYQIGHLSELLPLV 258 +P I PDY+I +L +V Sbjct: 206 DHNPGIIPDYEINSWKQLNDIV 227 >STM4559 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 226 Score = 82.8 bits (203), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 48/125 (38%), Positives = 72/125 (57%), Gaps = 4/125 (3%) Query: 136 TLQVLDELKGKVKLLLLTNGCPALQQEKLDGVPELVPYFDHVVISGNFGKGKPDKGIFTH 195 + +L+ ++G+ K+ ++TNG ALQQ +L+ L YFD +VIS G KPD IF + Sbjct: 101 AVSLLNAIRGQAKIGIITNGFTALQQTRLERTG-LREYFDLLVISEQVGVAKPDPKIFNY 159 Query: 196 AMNLLDITPEEG--IMVGDKLTTDILGGLGAGLTTVWINRTGKASDPEITPDYQIGHLSE 253 A+ P+ +MVGD +DILGG+ AGL+T W+N + I P + + LSE Sbjct: 160 ALEQAG-NPDRSRVLMVGDTAESDILGGINAGLSTCWLNAHHREQPAGIHPTWTVASLSE 218 Query: 254 LLPLV 258 L L+ Sbjct: 219 LEQLL 223 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,129,550 Number of extensions: 774616 Number of successful extensions: 1458 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 1442 Number of HSP's successfully gapped: 40 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)