BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0015.p (442 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2360 [E] COG0174 Glutamine synthetase 693 0.0 MA4216 [E] COG0174 Glutamine synthetase 540 e-153 STM0943 [K] COG1278 Cold shock proteins 518 e-146 BH3867 [E] COG0174 Glutamine synthetase 508 e-144 ML1631 [E] COG0174 Glutamine synthetase 384 e-106 >BH2360 [E] COG0174 Glutamine synthetase Length = 211 Score = 693 bits (1789), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/446 (0%), Positives = 0/446 (0%), Gaps = 6/446 (1%) Query: 3 FSKEDILRIAKDENVRFIRLQFTDLLGTIKNVEIPVSQLEKALDNKMMFDGSSIEGYVRI 62 + +IL ++ N + + +Q NK + Sbjct: 4 AKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAA 63 Query: 63 EESDMYLYPDLSTWVIFPWVTDS-RVARLICDVYMP-----DGTPFAGDPRGILKRCLQE 116 I + + + + + + A + + R Sbjct: 64 MLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTAN 123 Query: 117 AEEMGYTAMNVGPEPEFFLFKTDERGNPTTELNDQGGYFDLAPMDLGENCRREIVLTLEE 176 + + +Q L + L L Sbjct: 124 VVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHG 183 Query: 177 MGFEIEASHHEVASGQHEIDFKYADAIKAADQIQTFKLVVKTVARQHGLHATFMPKPLFG 236 Sbjct: 184 243 Query: 237 MNGSGMHAHQSLFKGNENVFYDESDTLGLSKTARYYMAGILKHARAFAAITNPTVNSYKR 296 Sbjct: 244 303 Query: 297 LVPGYEAPCYVAWSASNRSPMIRIPASRGLSTRIEVRNPDPAANPYLALAVMLKAGLDGI 356 Sbjct: 304 363 Query: 357 KRQLDLPAPIDRNIYVMSEEERIEEGIPSLPSDLKEALGEMIRSHVITEALGEHALAHFY 416 Sbjct: 364 423 Query: 417 ELKEIEWDIYRTQVHEWERDQYMTLY 442 Sbjct: 424 449 >MA4216 [E] COG0174 Glutamine synthetase Length = 252 Score = 540 bits (1390), Expect = e-153, Method: Compositional matrix adjust. Identities = 0/439 (0%), Positives = 0/439 (0%), Gaps = 2/439 (0%) Query: 4 SKEDILRIAKDENVRFIRLQFTDLLGTIKNVEIPVSQLEKALDNKMMFDGSSIEGYVRIE 63 ++ R+ ++ P + + I+ + Sbjct: 9 YLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMI 68 Query: 64 ESDMYLYPDLSTWVIFPW-VTDSRVARLICDVYMPDGTPFAGDPRGILKRCLQEAEEMGY 122 PD + LI P + L L + Sbjct: 69 AEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAV 128 Query: 123 TAMNVGPEPEFFLFKTDERGNPTTELNDQGGYFDLAPMDLGENCRREIVLTLEEMGFEIE 182 M + + + + + V + Sbjct: 129 Q-MPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQM 187 Query: 183 ASHHEVASGQHEIDFKYADAIKAADQIQTFKLVVKTVARQHGLHATFMPKPLFGMNGSGM 242 SH + + + Q F + + L + Sbjct: 188 PSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFIV 247 Query: 243 HAHQSLFKGNENVFYDESDTLGLSKTARYYMAGILKHARAFAAITNPTVNSYKRLVPGYE 302 Sbjct: 248 307 Query: 303 APCYVAWSASNRSPMIRIPASRGLSTRIEVRNPDPAANPYLALAVMLKAGLDGIKRQLDL 362 Sbjct: 308 367 Query: 363 PAPIDRNIYVMSEEERIEEGIPSLPSDLKEALGEMIRSHVITEALGEHALAHFYELKEIE 422 Sbjct: 368 427 Query: 423 WDIYRTQVHEWERDQYMTL 441 Sbjct: 428 446 >STM0943 [K] COG1278 Cold shock proteins Length = 73 Score = 518 bits (1333), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/437 (0%), Positives = 0/437 (0%), Gaps = 2/437 (0%) Query: 6 EDILRIAKDENVRFIRLQFTDLLGTIKNVEIPVSQLEKALDNKMMFDGSSIEGYVRIEES 65 + + FI + I + + + Sbjct: 4 GTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVRFDVHQGPKGNHASV 63 Query: 66 DMYLYPDLSTWVIFPWVTD-SRVARLICDVYMPDGTPFAGDPRGILKRCLQEAEEMGYTA 124 Sbjct: 64 123 Query: 125 MNVGPEPEFFLFKTDERGNPTTELNDQGGYFDLAPMDLGENCRREIVLTLEEMGFEIEAS 184 Sbjct: 124 - 182 Query: 185 HHEVASGQHEIDFKYADAIKAADQIQTFKLVVKTVARQHGLHATFMPKPLFGMNGSGMHA 244 Sbjct: 183 242 Query: 245 HQSLFKGNENVFYDESDTLGLSKTARYYMAGILKHARAFAAITNPTVNSYKRLVPGYEAP 304 Sbjct: 243 302 Query: 305 CYVAWSASNRSPMIRIPASRGLSTRIEVRNPDPAANPYLALAVMLKAGLDGIKRQLDLPA 364 Sbjct: 303 362 Query: 365 PIDRNIYVMSEEERIEEGIPSLPSDLKEALGEMIRSHVITEALGEHALAHFYELKEIEWD 424 Sbjct: 363 422 Query: 425 IYRTQVHEWERDQYMTL 441 Sbjct: 423 439 >BH3867 [E] COG0174 Glutamine synthetase Length = 252 Score = 508 bits (1308), Expect = e-144, Method: Compositional matrix adjust. Identities = 0/437 (0%), Positives = 0/437 (0%), Gaps = 5/437 (1%) Query: 6 EDILRIAKDENVRFIRLQFTDLLGTIKNVEIPVSQLEKALDNKMMFDGSSIEGYVRIEES 65 + V +++ LG + Q D S Sbjct: 16 RSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSAL 75 Query: 66 DMYLYPDLSTWVIFPWVTDSRVA--RLICDVYMPDGTPFAGDPRGILKRCLQEAEEMGYT 123 + + P + + + + L + Sbjct: 76 PDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWL 135 Query: 124 AMNVGPEPEFFLFKTDERGNPTTELNDQGGYFDLAPMDLGENCRREIVLTLEEMGFEIEA 183 ++ + L Y+D+ E + + Sbjct: 136 -EPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAII 194 Query: 184 SHHEVASGQHEIDFKYADAIKAADQIQTFKLVVKTVARQHGLHATFMPKPLFGMNGSGMH 243 Sbjct: 195 254 Query: 244 AHQSLF--KGNENVFYDESDTLGLSKTARYYMAGILKHARAFAAITNPTVNSYKRLVPGY 301 Sbjct: 255 314 Query: 302 EAPCYVAWSASNRSPMIRIPASRGLSTRIEVRNPDPAANPYLALAVMLKAGLDGIKRQLD 361 Sbjct: 315 374 Query: 362 LPAPIDRNIYVMSEEERIEEGIPSLPSDLKEALGEMIRSHVITEALGEHALAHFYELKEI 421 Sbjct: 375 434 Query: 422 EWDIYRTQVHEWERDQY 438 Sbjct: 435 451 >ML1631 [E] COG0174 Glutamine synthetase Length = 252 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 0/445 (0%), Positives = 0/445 (0%), Gaps = 9/445 (2%) Query: 5 KEDILRIAKDENVRFIRLQFTDLLGTIKNVEIPVSQLEKALDNKMMFDGSSIEGYVRIEE 64 + E R + + + + Sbjct: 5 NPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSS 64 Query: 65 SDMYLYPDLSTWVIFPWVTDS---RVARLICDVYMPDGTPFAGDPRGILKRCLQEAEEMG 121 M + D + + + ++ + D + + G Sbjct: 65 PAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQG 124 Query: 122 YTAMNVGPEPEFFLFKT--DERGNPTTELNDQGGYFDLAPMDLGENCRREIVLTLEEMGF 179 A+ P+ E + + + + Sbjct: 125 VLAV-QMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTY 183 Query: 180 EIEASHHEVASGQHEIDFKYADAIKAADQIQTFKLVVKTVARQHGLHATFMPKPLFGMNG 239 + H+ + Q L + + L Sbjct: 184 YHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPR 243 Query: 240 SGMHAHQSLFKGNENVFYDESDTLGLSKTARYYMAGILKHARAFAAITNPTVNSYKRLVP 299 Sbjct: 244 303 Query: 300 GYEAPCYVAWSASNRSPMIRIP---ASRGLSTRIEVRNPDPAANPYLALAVMLKAGLDGI 356 Sbjct: 304 363 Query: 357 KRQLDLPAPIDRNIYVMSEEERIEEGIPSLPSDLKEALGEMIRSHVITEALGEHALAHFY 416 Sbjct: 364 423 Query: 417 ELKEIEWDIYRTQVHEWERDQYMTL 441 Sbjct: 424 448 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,189,008 Number of extensions: 1473311 Number of successful extensions: 3020 Number of sequences better than 1.0e-05: 69 Number of HSP's gapped: 2773 Number of HSP's successfully gapped: 72 Length of query: 442 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 333 Effective length of database: 25,807,025 Effective search space: 8593739325 Effective search space used: 8593739325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)