BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0005.p (581 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pbpF [M] COG0744 Membrane carboxypeptidase (penicillin-bindin... 410 e-114 BH1201 [M] COG0744 Membrane carboxypeptidase (penicillin-binding... 376 e-104 lin2331 [M] COG0744 Membrane carboxypeptidase (penicillin-bindin... 335 2e-91 BS_ponA [M] COG0744 Membrane carboxypeptidase (penicillin-bindin... 318 1e-86 BH1702 [M] COG0744 Membrane carboxypeptidase (penicillin-binding... 308 2e-83 >BS_pbpF [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein) Length = 491 Score = 410 bits (1053), Expect = e-114, Method: Compositional matrix adjust. Identities = 0/515 (0%), Positives = 0/515 (0%), Gaps = 11/515 (2%) Query: 55 LAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLIGELALEKSEPVEYEDI 114 ++ L + K+ + + D Sbjct: 18 ASVPYMHQAALAAEKQDALSGQID--KILADHPALEGAMAGITVRSAETGAVLYEHSGDT 75 Query: 115 PKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGSTITQQLAKNIFLTRD 174 ++ G + + G + + + + Sbjct: 76 RMRPASSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDF 135 Query: 175 KTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAASIRYFGQSNLNDLKV 234 + V N + + + + + Sbjct: 136 DKMAEILKHSGVKVIKGNLIGDDTWHDDMRLSPDMPWSDEYTYYGAPISALT-ASPNEDY 194 Query: 235 WQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQ--MDEAKVVNYNYK 292 + + K + ++ ++ N Sbjct: 195 DAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKDLTIEREHGTNTITI 254 Query: 293 PPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAHAQETVEKAFADSDNFE 352 ++ + L ++ I D E + + Sbjct: 255 EGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTGEAPSSSDVLLSHRS 314 Query: 353 KSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSRRSPGSSFKPLVAYAPAL 412 + ++ + V + +K S G S+ + +L Sbjct: 315 MP--LSKLFVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEV-LNSTLPEFGVDSKSL 371 Query: 413 ESGKFTNNSVLSNEKQCFGTYCPNNLHG-YSKTISMAAALTKSENIPAVWLLNEIGVNTG 471 + S + + ++ + + + V + Sbjct: 372 VLRDGSGISHIDAVSSDQLSQLLYDIQDQSWFSAYLNSLPVAGNPDRMVGGTLRNRMKGT 431 Query: 472 FQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAFANGGELREAYSIKSITNSD 531 K +S G + + E + + Sbjct: 432 PAQGKVRAKTGSLS--TVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDIEDQIAVILA 489 Query: 532 GDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGT 566 Sbjct: 490 524 >BH1201 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein) Length = 719 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 197/504 (39%), Positives = 313/504 (62%), Gaps = 11/504 (2%) Query: 71 GEKLLDENIDKLTVNETTKIYDRNAKLIGELALEKSEPVEYEDIPKLLVNAFVATEDKRF 130 G+ ++DE +KL +N TT + D ++ L +E EPV D+P + +AF+A ED RF Sbjct: 30 GQYVIDE--EKLVMNATTTLVDTKGNVVSRLFIEDREPVSILDVPDHVKHAFIAIEDARF 87 Query: 131 FEHSGVDLWSIGRAAVKDIAARSMVEGGSTITQQLAKNIFLTRDKTFFRKATEVSIAVAL 190 +H G+D +IGRA +D+ A +EGGSTITQQL KN FL +KT+ RK EV IA+ L Sbjct: 88 HDHQGIDFRAIGRALYRDMLAGEKLEGGSTITQQLVKNAFLDHEKTWLRKTKEVLIAMNL 147 Query: 191 ENKFTKEEIITKYLNRINFGGTIYGIKAASIRYFGQSNLNDLKVWQIATLAAMPKGPSRY 250 E +++K I+ YLN+I FG YGI++A+ YF + +++DL V + A LA + K P+ Y Sbjct: 148 ERRYSKSAILEFYLNQIYFGHGAYGIQSAANLYFDK-DVSDLTVEEGAVLAGLAKAPTIY 206 Query: 251 NPLRNPELSKERRGVVLQLMYEQGYITKDQMDEAK--VVNYNYKPPESKQRYQAFIDYAV 308 +P+ + E SK+RR +VL +M QG+IT ++ AK ++ + P ++ Y +ID + Sbjct: 207 SPIIDLERSKQRRDLVLTVMQRQGFITAEEAVRAKGRLIQLDVNEPAEEEAYLTYIDMVI 266 Query: 309 DEAEDKFGLTEDDLNIGGYKIYTTMDAHAQETVEKAFADSDNFEKSADDELVQGSMTIIN 368 DEAE ++ L+ + GGY + +D QE + + K +D Q + +++ Sbjct: 267 DEAESQYHLSHQEFMTGGYTVVVPIDQAVQEVAFTLIHEKKYYPKGNED--AQSAFVLLD 324 Query: 369 QENGSIVALLGGRNYEKKGYSRVNGSRRSPGSSFKPLVAYAPALESGKFTNNSVLSNEKQ 428 + G ++A+ GG++Y ++G +RVN R+ PGS+FKPL +APALE G+ S+L +E + Sbjct: 325 AQTGGVLAVQGGKDYVRRGLNRVNVPRQ-PGSTFKPLAVFAPALEEGRAEPYSLLRDELR 383 Query: 429 CFGTYCPNNLHG-YSKTISMAAALTKSENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDK 487 + Y P+N Y + +++ A+T+S+N PAVWLLNE+G+ T Q+ ++ G +L DE Sbjct: 384 TYDGYEPHNFGDEYEEEVTLYDAITQSKNAPAVWLLNELGIPTAKQYLQRQGFQLDDE-- 441 Query: 488 NLSLALGGMSEGTNTLEMAQAYSAFANGGELREAYSIKSITNSDGDTVYKANTKPERVMS 547 L++ALGG+ +G LE+A AY FANGG+ RE Y I++I N +G+ + + + +V S Sbjct: 442 GLAIALGGLQDGVTPLELASAYQVFANGGKKREPYFIEAIYNREGEKMAEVDRTETQVYS 501 Query: 548 ENTAYQMTEMMQKVVEDGTGKKAK 571 TA+ MT M++ VV +GTG A+ Sbjct: 502 PQTAWSMTRMLESVVREGTGTSAQ 525 >lin2331 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein) Length = 714 Score = 335 bits (858), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 203/539 (37%), Positives = 322/539 (59%), Gaps = 14/539 (2%) Query: 42 KKRVLWTLFFTTALAIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLIGEL 101 K + + +F L + YL ++ K D + K + T +YD++ GEL Sbjct: 35 KNKHIGKIFLLAGLVFLLSFIIYLVVV---AKSADIDALKKGLESATIVYDKDGDKAGEL 91 Query: 102 ALEKSEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGSTI 161 + + V + I K L NA V+ ED+RF+EH G DL I RA V I + GGSTI Sbjct: 92 SSTDATFVSIDKISKNLQNAVVSIEDRRFYEHKGFDLKGIARAGVNLITNGGISGGGSTI 151 Query: 162 TQQLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAASI 221 TQQLAKN LT+++TF RKA E+ +A +E ++K+EI+ YLNR FG +G++ AS+ Sbjct: 152 TQQLAKNALLTQEQTFTRKAKEIFMAREIEKTYSKDEIMEMYLNRSYFGNGEWGVENASL 211 Query: 222 RYFGQSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQM 281 +YFG+ DL + + AT+A + + PS Y+P ++ E + RR +VL M E G ITK + Sbjct: 212 KYFGKP-AADLNIPEAATIAGLLQAPSAYDPYQHIEKATNRRNMVLNAMVETGDITKAEG 270 Query: 282 DEAK----VVNYNYKPPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAHA 337 D+ K V+N K P + +Y ++D ++EA ++ +T+D++ GYKIYT +D + Sbjct: 271 DKYKATKIVLNDQSKDPLA-NKYPWYVDAVINEAVNEADITQDEIMQKGYKIYTELDQNY 329 Query: 338 QETVEKAFADSDNFEKSADD-ELVQGSMTIINQENGSIVALLGGRN-YEKKGYSRVNGSR 395 Q ++EK + + F ++A+D LVQ +++ G I AL+GGR + +G++R + Sbjct: 330 QTSLEKVYNNDSLFPRNANDGTLVQSGAVLMDPATGGIRALVGGRGEHVFRGFNRATQMK 389 Query: 396 RSPGSSFKPLVAYAPALESGKFTNNSVLSNEKQCF-GTYCPNNLHG-YSKTISMAAALTK 453 PGS+ KPL Y PAL+SG + +S+L +EK + G Y P N+ G Y+ + M A+ Sbjct: 390 AQPGSTMKPLAVYTPALQSG-YNVDSMLKDEKVTYKGNYTPTNVGGIYNGEVPMYKAVAN 448 Query: 454 SENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQAYSAFA 513 S N PAVWLL++IG++ G + +K GI++ ++D+ L LALGGMS+G + +EMA AY+ FA Sbjct: 449 SINAPAVWLLDQIGIDKGVKSVEKFGIEVPEKDQTLGLALGGMSKGASPVEMATAYATFA 508 Query: 514 NGGELREAYSIKSITNSDGDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGTGKKAKI 572 N G E++ I I + G+TVY+ K ++++S+ + +MT M+ V+ GTG+ A + Sbjct: 509 NNGAKPESHIITKIVDPSGNTVYENVPKTKQIISKTVSTEMTSMLLDVINTGTGQSAAV 567 >BS_ponA [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein) Length = 914 Score = 318 bits (816), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 194/540 (35%), Positives = 306/540 (56%), Gaps = 26/540 (4%) Query: 48 TLFFTTALAIFCALGGYLF--IMLNGEKLLDENIDKLTVNETTKIYDRNAKLIGELALEK 105 TLF L + +GG + +M++ LDE+ KL ++ IYD+N K I E+ EK Sbjct: 37 TLFTLLILFVLGVVGGAVTFAVMVSDAPSLDES--KLKTPYSSTIYDKNGKEIAEVGAEK 94 Query: 106 SEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEGGSTITQQL 165 V ++IP ++ AF+ATED RF+EH G+D IG A V + EGGSTITQQ+ Sbjct: 95 RTYVSIDEIPDVVKEAFIATEDARFYEHHGIDPVRIGGALVANFKDGFGAEGGSTITQQV 154 Query: 166 AKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIKAASIRYFG 225 KN L+ KT RK EV +++ LE ++K+EI+ YLNRI F YGI A+ +FG Sbjct: 155 VKNSLLSHQKTLKRKVQEVWLSIQLERNYSKDEILEMYLNRIYFSPRAYGIGKAAEEFFG 214 Query: 226 QSNLNDLKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYITKDQMDEAK 285 ++L+ L V Q ATLA MP+ P+ YNP++NP+ +++RR +VL LM +QG+I+ Q ++AK Sbjct: 215 VTDLSKLTVEQAATLAGMPQSPTAYNPVKNPDKAEKRRNIVLSLMKKQGFISDSQYNKAK 274 Query: 286 --------VVNYNYKPPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTTMDAHA 337 VV+ S +Y AF++ + E ++K + D + G KIYTT+D A Sbjct: 275 KVAVKDEGVVSQKEYEKASTNKYSAFVEEVMKEIDEKSDV---DPSADGLKIYTTLDTKA 331 Query: 338 QETVEKAF-ADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYEKKGYSRVNGSRR 396 Q+ +++ D+ F E +QG +T+++ +NG + A+ GRN G++ ++ Sbjct: 332 QDKLDELMDGDTVGFT-----EGMQGGVTLLDTKNGEVRAIGAGRNQPVGGFNYATQTKA 386 Query: 397 SPGSSFKPLVAYAPALESGKFTNNSVLSNEKQCFGTYCP--NNLHGYSKTISMAAALTKS 454 PGS+ KP++ Y P +E+ K++ + + + P + Y ISM AL +S Sbjct: 387 QPGSTIKPILDYGPVIENKKWSTYEQIDDSAYTYSNGKPIRDWDRKYLGPISMRYALAQS 446 Query: 455 ENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGM--SEGTNTLEMAQAYSAF 512 NIPA+ +G +T FA LG+ L ++ + ++GG ++G + L MA AYSAF Sbjct: 447 RNIPALKAFQAVGKDTAVDFANGLGLGLTKDNVTEAYSIGGFGGNDGVSPLTMAGAYSAF 506 Query: 513 ANGGELREAYSIKSITNSDGDTVYKANTKPERVMSENTAYQMTEMMQKVVEDGTGKKAKI 572 N G E + +KSI +DG T K + MS+ TA+ +T+M++ V+ GTG+ A++ Sbjct: 507 GNNGTYNEPHFVKSIEFNDG-TKLDLTPKSKSAMSDYTAFMITDMLKTAVKTGTGQLAQV 565 >BH1702 [M] COG0744 Membrane carboxypeptidase (penicillin-binding protein) Length = 886 Score = 308 bits (789), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 190/546 (34%), Positives = 311/546 (56%), Gaps = 21/546 (3%) Query: 42 KKRVLWTLFFTTAL---AIFCALGGYLFIMLNGEKLLDENIDKLTVNETTKIYDRNAKLI 98 K+ +L LF L AI A G +F ++ +D +KL + +I+D++ + + Sbjct: 25 KRGILKKLFIAFILLFGAIMLAGGITVFAIIAQSPEIDP--EKLQFPQGAQIFDQSDEFV 82 Query: 99 GEL-ALEKSEPVEYEDIPKLLVNAFVATEDKRFFEHSGVDLWSIGRAAVKDIAARSMVEG 157 +L E+ V Y+DIP+ L AF++ ED RF +H G+DL IG A +I + EG Sbjct: 83 SQLDTSERRIQVNYQDIPEELEEAFISVEDVRFRDHFGIDLRRIGGAIAANITSGFGSEG 142 Query: 158 GSTITQQLAKNIFLTRDKTFFRKATEVSIAVALENKFTKEEIITKYLNRINFGGTIYGIK 217 STITQQL KN+FLT +K RK E +AV LE +++K +I+ YLN+I G +YG++ Sbjct: 143 ASTITQQLVKNVFLTNEKALTRKIQEQYLAVKLEQQYSKNQILEMYLNQIYLGQGVYGVE 202 Query: 218 AASIRYFGQSNLND-LKVWQIATLAAMPKGPSRYNPLRNPELSKERRGVVLQLMYEQGYI 276 AS YFG+S +D + A LAA+P+ PS Y+P+ NPE ++ RR +V+ +M +QG I Sbjct: 203 QASQYYFGKSITDDSFSLADAALLAAIPRRPSFYDPVVNPENAESRRNLVISMMADQGKI 262 Query: 277 TKDQMDEAKVV----NYNYKPPESKQRYQAFIDYAVDEAEDKFGLTEDDLNIGGYKIYTT 332 T ++ + A+ + N + ES ++AF + E E+ G++ +DL G KIYTT Sbjct: 263 TAEEAEAARSIPIAEQLNVQERESYP-FEAFYNQVHKEVEEMEGISVNDLYNAGLKIYTT 321 Query: 333 MDAHAQETVEKAFADSDNFEKSADDELVQGSMTIINQENGSIVALLGGRNYE--KKGYSR 390 +D AQ+ VEK + + + E Q +T+++ + G I A+ GR ++G++ Sbjct: 322 LDTEAQQHVEKVLQTDEFIQTYPNKEDFQAGITLVDTQTGEIKAIGSGRENTNVQRGFNY 381 Query: 391 VNGSRRSPGSSFKPLVAYAPALESGKFTNNSVLSNEKQCF--GTYCPNNLHGYSKTISMA 448 +R PGS+ KP++ Y P +E +++ ++ +E + G N Y ++M Sbjct: 382 ATDIKRQPGSTIKPILDYGPVIEYLQWSTGRIIKDEPHQYSNGVEFKNFDGAYKGNMTMR 441 Query: 449 AALTKSENIPAVWLLNEIGVNTGFQFAKKLGIKLKDEDKNLSLALGGMSEGTNTLEMAQA 508 AL +S N+PA+ L E+GV +FA+ +G+++ + + S LGG + G +TL+MA A Sbjct: 442 QALAESRNVPAIKALQEVGVERAAEFAESIGVEIDQKPMHESYGLGGFNTGVSTLDMAGA 501 Query: 509 YSAFANGGELREAYSIKSITNSDGDTVYKANTKPE--RVMSENTAYQMTEMMQKVVEDGT 566 Y+AF NGG+ E ++++ I DG + +T PE + MS+ TAY +++M++ VE GT Sbjct: 502 YAAFGNGGKYIEPHTVRKIEFPDGRVI---DTTPESTQAMSDYTAYMISDMLKTAVESGT 558 Query: 567 GKKAKI 572 G A I Sbjct: 559 GTVASI 564 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,614,651 Number of extensions: 1661712 Number of successful extensions: 3335 Number of sequences better than 1.0e-05: 102 Number of HSP's gapped: 2965 Number of HSP's successfully gapped: 105 Length of query: 581 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 469 Effective length of database: 25,461,023 Effective search space: 11941219787 Effective search space used: 11941219787 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 119 (50.4 bits)