BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0002.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2564 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 191 8e-49 STM0525 [G] COG1929 Glycerate kinase 181 1e-45 alr5266 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 155 4e-38 AGc3695 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 121 1e-27 >RSc2564 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Length = 323 Score = 191 bits (486), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 119/238 (50%), Positives = 142/238 (59%), Gaps = 9/238 (3%) Query: 5 TRRRVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIPH 64 T R + LLGPTA GKT +L LAE + EIIS DS VYR MDIGTAK E PH Sbjct: 7 TAPRAVCLLGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAEQAIAPH 66 Query: 65 HLIDIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVAD 124 HLIDI DP DAYSAA+F LIE I +G+LP IVGGT LY ++L G AD Sbjct: 67 HLIDIIDPLDAYSAAQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSDLPG-AD 125 Query: 125 EAFRSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSA-- 182 A R+E DA A G ALHA+LA+VDP +AARLH D +RI RALE++ T +SA Sbjct: 126 PAIRAEIDAEAARDGWPALHAKLAQVDPVTAARLHATDAQRIQRALELYRLTGQPMSALL 185 Query: 183 -----SHADQKKESPYELCLIGL-TMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRG 234 + A + E+ I L DR +L+ RI R D MLA GL+ EVE L +RG Sbjct: 186 AREAGAAAFHRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLAGGLLDEVEALRRRG 243 >STM0525 [G] COG1929 Glycerate kinase Length = 382 Score = 181 bits (458), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 12/232 (5%), Positives = 33/232 (14%), Gaps = 4/232 (1%) Query: 8 RVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIPHHLI 67 ++++ + A + D+ V + G + + Sbjct: 2 KIVIAPDSFKESLSADKCCQAIKAGFSTVFPDARYVCLPIADGGEGTVDAMVAATGGKRV 61 Query: 68 DIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVADEAF 127 + T + + + + Sbjct: 62 SVDVSGPMGEKVNGFYGLTGDGKTAIIEMAAASGLMLVAPEARNPLLAS-SFGTGELIRH 120 Query: 128 RSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSASHADQ 187 + A + + A + S Sbjct: 121 ALDAGIR-HIILGIGGSATVDGGMGVAQALGVRFLDAQGTPLGAGGGNLSRLASIDLQGC 179 Query: 188 KKESPYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQRGYSRSL 239 C I + D R+ Sbjct: 180 DPRISE--CRIEVACDVDNPLVGPRGAAAVFGPQKGATPEMVETLENGLRNY 229 >alr5266 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Length = 359 Score = 155 bits (393), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 14/228 (6%), Positives = 40/228 (17%), Gaps = 12/228 (5%) Query: 8 RVLVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIPHHLI 67 V++ A+ L + + G+ + + G+ Sbjct: 3 AATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEE 62 Query: 68 DIHDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVADEAF 127 + + +G ++ E L S + Sbjct: 63 ALRLRAGGITKPVLLLEGFFDARDLPTISAQ--HFHTAVHNEEQLAALEEASLDEPVTVW 120 Query: 128 RSEQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSASHADQ 187 + AE + + I + + + Sbjct: 121 MKLDTGM----HRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGAT----E 172 Query: 188 KKESP--YELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLLQR 233 K+ + + + G+ Sbjct: 173 KQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPL 220 >AGc3695 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Length = 301 Score = 121 bits (304), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 16/228 (7%), Positives = 39/228 (17%), Gaps = 11/228 (4%) Query: 10 LVLLGPTAVGKTRLSLELAEAYDAEIISGDSMQVYRGMDIGTAKIKPEEMMGIPHHLIDI 69 VG A D + + + + A ++ + + Sbjct: 40 AQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGT 99 Query: 70 HDPQDAYSAAEFQEQGTKLIEEISRKGKLPFIVGGTGLYIESLCYGFRFSEAVADEAFRS 129 D +G + K +++ L F + V + Sbjct: 100 RWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPIS-AET 158 Query: 130 EQDAFAEAHGAEALHARLAEVDPESAARLHPNDRRRIIRALEIHHQTKVTLSASHADQKK 189 + A PE E + Sbjct: 159 GLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAEL----PYSV 214 Query: 190 ES------PYELCLIGLTMDRKILYKRIEDRIDQMLADGLVAEVEGLL 231 E + + + + + + Sbjct: 215 TVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEAR 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,028,227 Number of extensions: 803763 Number of successful extensions: 2755 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2747 Number of HSP's successfully gapped: 4 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)