BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0006.p (527 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN0649 [R] COG1574 Predicted metal-dependent hydrolase with the ... 107 6e-23 BMEII0123 [R] COG1574 Predicted metal-dependent hydrolase with t... 106 8e-23 SMa0101 [R] COG1574 Predicted metal-dependent hydrolase with the... 100 5e-21 RSp1459 [R] COG1574 Predicted metal-dependent hydrolase with the... 93 1e-18 RSc0239 [R] COG1574 Predicted metal-dependent hydrolase with the... 88 3e-17 >FN0649 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Length = 542 Score = 107 bits (266), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 116/552 (21%), Positives = 221/552 (40%), Gaps = 56/552 (10%) Query: 10 DRVLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPGV 69 D++ ++ +++ ++ + ++ +I +G EAK+ + K + LK +++PG+ Sbjct: 3 DKLFINGEIYSMKKEGEKFQSLGVKDGKIVFLGTNDEAKN-VSSKELID-LKGKMMIPGM 60 Query: 70 HDNHVFFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYAYGWSEEGW--GQL 127 D H+ Y VDL+ I+E + L+++ +N + + + W + Sbjct: 61 ADAHLHLYAYCQNLTFVDLSKVHDINEMVSLMKEKVKNIKKGDWIKGVNFDQSKWKENRF 120 Query: 128 PGQSLLDEAFPDRAVIAINRTKSYCWMNTLAQHK----------YQFA------------ 165 P +D D VI K C +A K YQ Sbjct: 121 PTLQEMDSISKDNPVI----IKRCCLHAVVANSKALEMAGIGKNYQAGSGGIVELDKDGM 176 Query: 166 PD----ECSAESRAHLLKDMMQDRELVRQEFLDFARMLAKRGVTSIKDIGFD--RHSXXX 219 P+ E S ++ +L D ++D E+ ++ D ++ +G+T+I +++ Sbjct: 177 PNGILREQSTKAFDDILPDPLKDIEVQKKIMQDVLNDMSSKGITTIHTYAAKIWQYNEDI 236 Query: 220 XXXXXXXXXXXXXXRVHFALEPVLQPLDIPAGLQYKERYQGEFLRFQ--GYKLMLDGVVA 277 RV ++ + +P + +E+ + + Q YK+ DG + Sbjct: 237 SIYKNFEKEEKLPLRVTVCIDELFEP-----EILTEEKLNNPYRKVQLGAYKIFSDGSMG 291 Query: 278 DHTGDMLEPYADMPGVTTLRPVDYEAIETAVQAADSHGIKCCLTAEGDAAIRKAVDILE- 336 + + PY D P + E + + A HG++ + A GD A+ + +E Sbjct: 292 SRSAALKAPYTDDPENSGFMLFTQEELNNKILTAYEHGLQPAIHAIGDRALDMTLAAIEY 351 Query: 337 --------GCSRRRGDQRIRHSISDLEYPHPDDIRRMGENGIFAEVYAQILLLNPSYEEA 388 G + +R+ I ++ + + RM + + ++ L + + E Sbjct: 352 TLKTTKEKGMTDEEQKKRLPFRIIHVQMIDDNLLERMKKLPLVLDIQPIFLCTDLHWIED 411 Query: 389 YMSAVAGKENESRFYNYKSMLEANVPVTIGTDLPLFLTSVPDSLYAASFRLFSDGSPMGG 448 + GKE + K+M A + T G+D P+ +YAA R +G P G Sbjct: 412 RI----GKERLKGSFALKTMENAGLIQTGGSDCPVETYEPLKGIYAAVTRQDMEGYPTEG 467 Query: 449 WQPEQGMPAAEVLKAWTINGARHNGMEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAH 508 + PE+ + E L +T N G E GTLE GK AD+ V +++L TEI+D Sbjct: 468 FLPEERLSIYEALCMYTKNVHYATGQESVLGTLEIGKFADLTVLEKDLFKIDETEIKDVK 527 Query: 509 VILTITAGQITY 520 V T AG + Sbjct: 528 VEQTYVAGNCVF 539 >BMEII0123 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Length = 560 Score = 106 bits (265), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 132/565 (23%), Positives = 232/565 (41%), Gaps = 60/565 (10%) Query: 1 MEAATHGFADRVLLSNAVFTGIEDSVSP--AFVALQGERIAAVGNPAEAKDWIGPKTFVH 58 M +ATH AD +L + ++T D+ P VA+ G RI AVG+ AE + + +T V Sbjct: 9 MTSATH--ADLILTNGRIYT--VDTARPWAQAVAISGGRILAVGSTAEIEAYRTGETEVV 64 Query: 59 HLKDALIMPGVHD--NHVFFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYA 116 L + MPG D NH+ G + L A++D+ +R+ AE Q + A Sbjct: 65 DLGGRMAMPGFVDVHNHIMMGGQADLFE-TQLPAGASVDDICAHVRRQAEAAVPGQWIIA 123 Query: 117 YGWSEEGWGQLPGQS---LLDEAFPDRAVIAINRTKSYCWMNTLAQHKYQFAPDE----- 168 W + L ++ LD A V+ + T W+N++A A D+ Sbjct: 124 NQWGADMITALNSEASLAKLDAASLGHPVLLRDETMHNRWVNSVALQMCGIANDQPDPPA 183 Query: 169 --------------CSAESRAHLLKDMMQDRELVRQEFLDFAR---MLAKRGVTSIKDIG 211 ES A +++ + D AR ++ GVT+ +D Sbjct: 184 GSFGRDPATGRLTGLLVESAAGIVERVAADHFTEAMGEAAIARAVEIVNSYGVTAFQDA- 242 Query: 212 FDRHSXXXXXXXXXXXXXXXXXRVHFALE--PVLQP-----LDIPAGLQYKERYQGEFLR 264 + +A+ P+++P L L ++ Y+ +R Sbjct: 243 ----ASVLPIMKALTGLDNKGKLTAWAVTSLPLIEPSFMFGLSGDELLALRDEYRTRHVR 298 Query: 265 FQGYKLMLDGVVADHTGDMLEPYAD----MPGVTTLRPVDYEAIETAVQAADSHGIKCCL 320 K+ LDGV T + Y D G V Y + + ++ G+ + Sbjct: 299 PNFTKIFLDGVPGARTSAFHDAYLDDGVHPRGYRGETLVTYPDLVQHIDKSEKLGMGLKI 358 Query: 321 TAEGDAAIRKAVDILEGCSRRRGDQRIRHSISDLEYPHPDDIRRMGENGIFAEVYAQILL 380 GD A+ + +D +E G ++ H I+ Y P D+ R + + A++ + ++ Sbjct: 359 HCTGDYAVTEMLDAVEAVRHFNGPAKVMHHIAHASYVRPQDVERFAKLSVVADL-SPMIW 417 Query: 381 LNPSYEEAYMSAVAGKENESRFYNYKSMLEANVPVTIGTDLPLFLTSVPDSLYAASFRLF 440 ++ E + A+ G E +RF+ + ++ + G+D P+ VPD + + Sbjct: 418 YPTTFLEGHKEAM-GDERATRFWPIADLHKSGALLAGGSDWPVI--PVPDP-WTGIEGMV 473 Query: 441 SDGSPMGGWQ-----PEQGMPAAEVLKAWTINGARHNGMEERTGTLEAGKAADIAVFDRN 495 + +P G + PEQ + A ++ +T+N A G+ TG++E GK+ADI V D+ Sbjct: 474 TRQNPSGAFPGVALWPEQAIDLATAIRIFTLNSAIAMGIGAETGSIEPGKSADIIVLDQQ 533 Query: 496 LLDTTATEIRDAHVILTITAGQITY 520 + D I D V+ T AG+ Y Sbjct: 534 VFDVAPDRIADTKVLTTYFAGRAVY 558 >SMa0101 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Length = 541 Score = 100 bits (250), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 134/544 (24%), Positives = 210/544 (38%), Gaps = 40/544 (7%) Query: 9 ADRVLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPG 68 AD +L++ ++ G E+ +S A QG +I A G+ + GP+T V L+ PG Sbjct: 7 ADLILINGRIWRGREEGISEALAVWQG-KILATGSDTDILGLKGPRTEVIDLEGRFATPG 65 Query: 69 VHDNHVFFTGYMSMHRGVDLTHAA--TIDEALQLLRQDAENRPSEQNVYAYGWSEEGW-- 124 + DNH+ VD T A+ T+ + + A P V A G+ + Sbjct: 66 LIDNHLHLIATGMAMGWVDATPASAPTLAALMGRISDRAATTPKGGWVRARGYDQVKLDT 125 Query: 125 GQLPGQSLLDEAFPDRAVIAINRTKSYCWMNT----LAQHKYQFAPDECS---------- 170 G+ P + LD PD V+ N+ LA A E Sbjct: 126 GRHPTRDDLDRVAPDHPVLLTRACGHVSIANSRALELAGITEATAVPEGGVIGVTEGRLN 185 Query: 171 ---AESRAHLLKDMMQDRELVRQEFLD----FARMLAKRGVTSIKDIGFDRHSXXXXXXX 223 AE+ +L+K M ++ +D R L G+TS D + S Sbjct: 186 GFLAENAQNLVKAAMP--SATTEDLIDGIERAGRYLLSFGITSCMDAAVGQVSGFAEIQA 243 Query: 224 XXXXXXXXXXRVHFALEPVLQP-LDIPAGLQYKERYQG---EFLRFQGYKLMLDGVVADH 279 V L + P + I G + LR G K+ LDG Sbjct: 244 YEMAKLSGRLPVRVWLTLLGDPGVSIVEDCWRAGLLSGAGDDMLRVGGVKVFLDGSAGGR 303 Query: 280 TGDMLEPYADMPGVTTLRPVDYEAIETAVQAADSHGIKCCLTAEGDAAIRKAVDILEGCS 339 T M PY P ++ + +E V+A G + A GD AI + + E Sbjct: 304 TAWMTRPYRGEPDNIGVQMLPDAEVEAVVKACHDRGYQMVCHAIGDGAIEQLITAYEKAL 363 Query: 340 RRRGDQRIRHSISDLEYPHPDDIRRMGENGIFAEVYAQILLLNPSYEEAYMSAVAGKENE 399 D RH + + PD RM GI Q+ ++ + ++Y+S V G+E Sbjct: 364 AANPDPDRRHRVEHCGFSTPDQNARMKAAGILPA--PQMAFIH-DFGDSYIS-VLGEERG 419 Query: 400 SRFYNYKSMLEANVPVTIGTDLPLFLTSVPDSLYAASFRLFSDGSPMGGWQPEQGMPAAE 459 Y + + + + G+D P+ +L+A R G+ M + + + E Sbjct: 420 RLSYPIGTWMRMGLKPSTGSDSPVCSPDPFPNLHAMLTRQTGKGTVM---EASERLSRQE 476 Query: 460 VLKAWTINGARHNGMEERTGTLEAGKAADIAVFDRNLLDTTA-TEIRDAHVILTITAGQI 518 L+ +T GA E G L G+ ADIAVFD +LL T + D +LT+ AG++ Sbjct: 477 ALQTYTEYGAYSQKAEGVKGRLVPGQWADIAVFDNDLLAAPPETILSDTSCVLTLLAGRV 536 Query: 519 TYDA 522 +DA Sbjct: 537 VHDA 540 >RSp1459 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Length = 574 Score = 93.2 bits (230), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 129/530 (24%), Positives = 203/530 (38%), Gaps = 46/530 (8%) Query: 31 VALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPGVHD--NHVFFTGYMSMHRGVDL 88 VA++ R A+G+ AE +GP T V L A+++PG+ D H Y +++ L Sbjct: 52 VAVRDGRFVAIGSDAEVAQLVGPATQVVDLHGAMVVPGLSDVHAHPLEGAYEALYH-CAL 110 Query: 89 THAATIDEALQLLRQDAENRPSEQNVYAYGWSEEGWGQL-PGQSL--LDEAFPDRAVIAI 145 +D L + A + + WS G L +SL LD A V+ Sbjct: 111 APTGDLDAVLAAVGACAARAQPDDWIVGGPWSSALLGALETPESLAALDRASKGLPVLLR 170 Query: 146 NRTKSYCWMNTLAQHKYQFAPDECSAESRAHLLKD------------------MMQDREL 187 + T W N++ + P + A+++ D ++ R Sbjct: 171 DDTFHNRWANSVVLQRAGIGPGAAAPAGGAYVMADGKPTGLIKEPPAWQPVEKLIPPRTP 230 Query: 188 VRQEFLD--FARMLAKRGVTSIKDIGFDRHSXXXXXXXXXXXXXXXXXRV-HFALEPVLQ 244 R + A L G+T ++D R V +EP Sbjct: 231 ARLKHAAEAAAHRLNSFGITGVQDAFVTRQILTGWHAADTDGQGLPLRVVASMGVEPEAG 290 Query: 245 PLDIPAGLQYKERYQGEFLRFQGYKLMLDGVVADHTGDMLEPYA-------DMPGVTTLR 297 +P Q + + + LR K+ LDGV +T MLEPYA D G Sbjct: 291 GDGLP-DRQTLAQVRSDRLRPDFVKVFLDGVPMSYTAAMLEPYAPSDAHGHDFRGA---- 345 Query: 298 PVDYEAIET-AVQAADSHGIKCCLTAEGDAAIRKAVDILEGCSRRRGDQRIRHSISDLEY 356 P+ A+ T + A D G L A GD A+R A+D +E + G RH I+ + + Sbjct: 346 PLYALAVLTEKLTALDRQGTPVKLHAVGDGAVRLALDAIEAVRKANGMNGPRHQIAHITF 405 Query: 357 PHPDDIRRMGENGIFAEVYAQILLLNPSYEEAYMSAVAGKENESRFYNYKSMLEANVPVT 416 P D+ R + A+V +L P V G Y S++ A V Sbjct: 406 IAPSDMPRFARLNVVADV--SPMLWFPHAYTPLFEKVVGHARTLHSYPIGSLIRAGTLVA 463 Query: 417 IGTDLPLFLTSVPD---SLYAASFRLFSDGSPMGGWQPEQGMPAAEVLKAWTINGARHNG 473 G+D P PD + R G+ G + + + L+ +T+N A G Sbjct: 464 GGSDWPAG-QQTPDPWLGIEGLVTRRNPAGNVPGVLDAHEAIRLGQALRLYTLNSATAMG 522 Query: 474 MEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAHVILTITAGQITYDAA 523 + + TG++E GK+AD AV ++L + I V +T G+ Y AA Sbjct: 523 LGQETGSIEVGKSADFAVLSQDLFKIPPSRIHRTTVRVTYFQGRPVYRAA 572 >RSc0239 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Length = 612 Score = 88.2 bits (217), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 130/550 (23%), Positives = 213/550 (38%), Gaps = 52/550 (9%) Query: 9 ADRVLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPG 68 AD +L + + T D + + VA++ R AVG+ A A GP T V L+ ++PG Sbjct: 3 ADLILHNGRIHTVDRDRPTASAVAVRDGRFVAVGDDATAMAQRGPATRVIDLRGRTVIPG 62 Query: 69 VHDNHV-FFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYAY-GWSEEGWGQ 126 ++D+H+ G ++ + + ++ +AL++LR+ A PS Q V GW+E + + Sbjct: 63 LNDSHLHLIRGGLNYNLELHWEGVPSLADALRMLREQAARTPSPQWVRVVGGWTEFQFAE 122 Query: 127 --LPGQSLLDEAFPDRAVIAINRTKSYCWMNTLAQHKYQFAPDECS-------------- 170 +P L++A PD V ++ +N A + + D + Sbjct: 123 RRMPTLDELNQAAPDTPVFVLH-LYDRALLNRAALRQVGYTRDTPNPPGGEIQRDAGGEP 181 Query: 171 -----AESRAHLLKDMMQDRELVRQEF-----LDFARMLAKRGVTSIKDI--GFDRHSXX 218 A A +L + + E+ F R L + GVTS D GF + Sbjct: 182 TGMLIARPNAMILYATLAKGPKLPPEYQVNSTRQFMRELNRLGVTSAIDAGGGFQNYPED 241 Query: 219 XXXXXXXXXXXXXXXRVHFAL---EPVLQPLDIPAGLQYKERYQG-EFLRFQGYKLMLDG 274 R+ + L +P + D QG +FLR G ML Sbjct: 242 YTVIRQLAQENQLTVRIAYNLFTQKPKEELADFKHWTGSVPYRQGDDFLRHNGAGEMLVF 301 Query: 275 VVADHTGDMLEPYADMPGVTTLRPVDYEAIETAVQAADSHGIKCCLTAEGDAAIRKAVDI 334 AD D L+P D+P T+ P +E V+ + L A D +I + +D+ Sbjct: 302 SAADFE-DFLQPRPDLP--ETMEP----ELEGVVRHLVAQRWPFRLHATYDESISRMLDV 354 Query: 335 LEGCSRRRGDQRIRHSISDLEYPHPDDIRRMGENGIFAEVYAQILLLNPSYEEAYMSAVA 394 E R + E P +I R+ G + ++ + E Y +A A Sbjct: 355 FERVDREIPFDGLPWFFDHAETISPKNIERVRALGGGIAIQDRMAFQGEYFVERYGAAAA 414 Query: 395 GKENESRFYNYKSMLEANVPVTIGTDLPLFLTSVPDSLYAASFRLFSDGSPMGGWQP-EQ 453 G+ + ML VPV GTD T V S G +GG Q Sbjct: 415 GQTPP-----IQRMLAEGVPVGAGTD----ATRVSSYNPWTSLYWLVSGRTVGGLPLYPQ 465 Query: 454 GMPAAEVLKAWTINGARHNGMEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAHVILTI 513 G+P L +T A + + G ++ G+ AD+A + I+ +LT+ Sbjct: 466 GLPRDVALALYTHGSAWFSADQGNKGQIKVGQLADLAALSADFFSVDEAGIKAIESVLTV 525 Query: 514 TAGQITYDAA 523 G++ Y AA Sbjct: 526 VDGRVVYGAA 535 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,466,904 Number of extensions: 1531266 Number of successful extensions: 3137 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 3126 Number of HSP's successfully gapped: 15 Length of query: 527 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 416 Effective length of database: 25,576,357 Effective search space: 10639764512 Effective search space used: 10639764512 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)