BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0003.p (321 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3601 [M] COG2222 Predicted phosphosugar isomerases 307 1e-83 STM0572 [M] COG2222 Predicted phosphosugar isomerases 286 3e-77 STM4540 [M] COG2222 Predicted phosphosugar isomerases 138 1e-32 SMc03139 [M] COG2222 Predicted phosphosugar isomerases 129 8e-30 ZyhfN [M] COG2222 Predicted phosphosugar isomerases 98 2e-20 >STM3601 [M] COG2222 Predicted phosphosugar isomerases Length = 325 Score = 307 bits (787), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 151/316 (47%), Positives = 207/316 (65%), Gaps = 2/316 (0%) Query: 1 MNVNQVVQSIREKVAE--GISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFV 58 M + + V +I AE G+ VY+VACGGS YP+K FL+ EAK L GLY + EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGVKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 59 HALPKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLY 118 + P LG +VV+V SH GNTPE++ AA+ A++ GA IGLT ++ L+ + DY Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETY 121 Query: 119 EWGDDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARV 178 +GD ++ L AV+LL EGYAH + FQ G+ IN I+ + +QVA RA+ Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 179 FADKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETP 238 FA +Y+++++ Y + SGA YG AY +IC MEMQW+++A IHSGE+FHGPFE+TD TP Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 239 FILMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICV 298 F +EG TRA+DERA FL +Y ++EV+DAKELG+ TI V+++FN LF ++ V Sbjct: 242 FFFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 299 YREALAEVRNHPLETR 314 Y ALAE R HPL TR Sbjct: 302 YNRALAEARQHPLTTR 317 >STM0572 [M] COG2222 Predicted phosphosugar isomerases Length = 328 Score = 286 bits (732), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 143/316 (45%), Positives = 204/316 (64%), Gaps = 5/316 (1%) Query: 2 NVNQVVQSIREKVAEGISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHAL 61 N +++ I K + I V+FV CGGSL +P KYFLD EA KL G T+NEFVHA Sbjct: 7 NARRIISDILGK--QNIERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHAT 64 Query: 62 PKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEWG 121 PK LG SVVI+ S GNT E+VAAA+ A+E+GA TIGL + + L YSDY Y+W Sbjct: 65 PKALGKNSVVILASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWA 124 Query: 122 ---DDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARV 178 + + A L LA+++L EGYA + + +++ ++QV A+ Sbjct: 125 RYPETVDPAQQKAAYSLWLALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQR 184 Query: 179 FADKYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETP 238 FA ++++E++ Y++ SG S+G A+ +IC L+EMQW+N+A+IHSGEYFHGPFE+T+ TP Sbjct: 185 FAAEWKDEKVVYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTP 244 Query: 239 FILMMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICV 298 FIL+ + GRTR LD+RA F+ +Y K+++IDA +LGI ++ +V E+F +L ++ V Sbjct: 245 FILLQSSGRTRPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDV 304 Query: 299 YREALAEVRNHPLETR 314 Y ALA RNHPL TR Sbjct: 305 YNLALATARNHPLTTR 320 >STM4540 [M] COG2222 Predicted phosphosugar isomerases Length = 343 Score = 138 bits (348), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 89/284 (31%), Positives = 136/284 (47%), Gaps = 12/284 (4%) Query: 17 GISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHALPKRLGAQSVVIVCSH 76 G S ++F + GGSL M F E L + A E +H KRL SVVI S Sbjct: 41 GFSSLFFASVGGSLAPMMAINEFA-KELTTLPVYVEQAAELIHKGNKRLNKDSVVITLSK 99 Query: 77 GGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEWGDDSNVKNN---PMAI 133 G+T ESVA A+ K QG + +T N ++ L + W KN + Sbjct: 100 SGDTKESVAIAEWCKAQGIRVVAITKNADSPLAQAA------TWHIPMRHKNGVEYEYML 153 Query: 134 ILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARVFADKYRNEELYYILT 193 + L ++L +A + F LE + + + K++ P+A A +Y N + Y + Sbjct: 154 LYWLFFRVLSRNNEFASYDRFASQLEILPANLLKAKQKFDPQADAIASRYHNSD-YMMWV 212 Query: 194 SGAS-YGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETPFILMMNEGRTRALD 252 GA +G Y F++C L EMQW + S E+FHG E+ +K+ P IL+ EG+ RALD Sbjct: 213 GGAEMWGEVYLFSMCILEEMQWKRTRPVSSAEFFHGALELLEKDVPLILVKGEGKCRALD 272 Query: 253 ERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSII 296 ER + F ++ + + VID K + I DE P + +++ Sbjct: 273 ERVERFASKITDNLVVIDPKAYALDGIDDEFRWIMAPCVVSTLL 316 >SMc03139 [M] COG2222 Predicted phosphosugar isomerases Length = 337 Score = 129 bits (323), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 83/313 (26%), Positives = 152/313 (48%), Gaps = 5/313 (1%) Query: 2 NVNQVVQSIREKVAEGISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHAL 61 ++ VV S EK AE V+F+ GG+ + M P+ L ++ A + E Sbjct: 22 RLDDVVVSCLEKGAEN---VFFLGTGGAAILMQPAAQLLQRRSR-FPAFIDLPAELFITG 77 Query: 62 PKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEWG 121 L +S+V++ S G T ESVA AKE GA + L ++E L D++F+ Sbjct: 78 SVNLTEKSIVVIPSLSGTTKESVALLAKAKEAGATVLSLVGHEETPLGKGGDHAFVNFAE 137 Query: 122 DDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARVFAD 181 DD++ ++ + + +A+ ++ + + LE + G++ + K+ +A FA Sbjct: 138 DDTSCESFYLQSLF-IALSIMRHRGEIENYDQIAGELERLPGLLLEVKRGYEDKAETFAR 196 Query: 182 KYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETPFIL 241 + + + +G ++ A+ + +C L EMQW+ +H+ ++FHG E+ +K IL Sbjct: 197 VLAGSDYHIVTGAGNAWPEAFYYGMCILEEMQWIRTRPVHASDFFHGTLELVEKGVSVIL 256 Query: 242 MMNEGRTRALDERAQTFLNQYAEKVEVIDAKELGIGTIADEVVEFFNPVLFYSIICVYRE 301 E R L +R Q F Y +++ V+D + + I+ EV +P++ +++ Sbjct: 257 FKGEDALRPLADRVQNFAPNYTDRLTVLDTADFALPGISQEVRGLVSPIVLATVLERISA 316 Query: 302 ALAEVRNHPLETR 314 L +RNHPL TR Sbjct: 317 HLEVMRNHPLTTR 329 >ZyhfN [M] COG2222 Predicted phosphosugar isomerases Length = 347 Score = 97.8 bits (242), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 64/269 (23%), Positives = 114/269 (42%), Gaps = 4/269 (1%) Query: 2 NVNQVVQSIREKVAEGISEVYFVACGGSLVDMYPSKYFLDSEAKKLVAGLYTANEFVHAL 61 +V V + E V I +YFVACG L +K+ D + V + + EF Sbjct: 35 DVPLVHAIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAI-SGWEFCDNT 93 Query: 62 PKRLGAQSVVIVCSHGGNTPESVAAAQTAKEQGAHTIGLTHNKEAALLNYSDYSFLYEWG 121 P RL + VI S G T E + A + + GA T T ++ + + +++S Y+ Sbjct: 94 PYRLDDRCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQ-- 151 Query: 122 DDSNVKNNPMAIILDLAVQLLHVAEGYAHVEAFQKGLESINGIINQGKKQVAPRARVFAD 181 + + + + ++++ A + + L+ + + + + R + Sbjct: 152 -ADCIWEIHLLLCYSVVLEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGE 210 Query: 182 KYRNEELYYILTSGASYGHAYGFAICSLMEMQWLNAAAIHSGEYFHGPFEVTDKETPFIL 241 + Y + +G Y I +LME W + I SGE+ HGP E+ + PF+ Sbjct: 211 LASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLF 270 Query: 242 MMNEGRTRALDERAQTFLNQYAEKVEVID 270 ++ +R ERA F+ Q + V VID Sbjct: 271 LLGNDESRHTTERAINFVKQRTDNVIVID 299 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,078,856 Number of extensions: 908984 Number of successful extensions: 2046 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2040 Number of HSP's successfully gapped: 13 Length of query: 321 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 215 Effective length of database: 26,153,027 Effective search space: 5622900805 Effective search space used: 5622900805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)