BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0053.p (239 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yobT [R] COG0491 Zn-dependent hydrolases, including glyoxylases 268 6e-72 lin1904 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 193 2e-49 FN1162 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 86 4e-17 PH0096 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 79 6e-15 BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases 78 1e-14 >BS_yobT [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 233 Score = 268 bits (684), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 133/232 (57%), Positives = 164/232 (70%) Query: 1 MRLTQEGQLLQLTWLPRLFPVNCYLVEEKEGLTLIDAAMSFSVQGILDTAAKLNKPINRI 60 MR+TQ + QLT+ P LFPVNCYLVEE+ +TLIDAA+ S +GI+ +L KP+ I Sbjct: 1 MRITQYQTVWQLTFFPALFPVNCYLVEEENEVTLIDAALPGSYKGIIQAVNQLGKPLQHI 60 Query: 61 ILTHAHGDHVGALDELKQRLPSAQVYISERDAALLRGDRSLREGEPQTPIKGSVPTKITT 120 +LTHAHGDHVG+LD L Q P A+V ISERD+ LL+GD SLR+ EPQTPIKG +P I T Sbjct: 61 LLTHAHGDHVGSLDTLAQTFPHAKVMISERDSFLLQGDTSLRQDEPQTPIKGGIPKHIQT 120 Query: 121 VPDILLQEGDAVGSLTAIATPGHTPGSMSFLDERTGALIAGDALQTFRRTAVAGTVVPFF 180 P LL G+ +GSL AI TPGHTPGSMSFLD R G LIAGDA Q AV+G + F Sbjct: 121 KPHQLLTGGETIGSLLAIPTPGHTPGSMSFLDTRNGTLIAGDAFQLRGGIAVSGQIKWAF 180 Query: 181 PFPAMATWNKASALESAVKLLKLSPKVLAVGHGNLLHAPIEHMKYAVEQAEQ 232 PFPA ATWNK A++SA L +P LAVGHG L +P E M+ A+++A++ Sbjct: 181 PFPAFATWNKEEAIKSAQLLADKAPSCLAVGHGKFLRSPSERMRQAIQKAKK 232 >lin1904 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 238 Score = 193 bits (491), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 98/213 (46%), Positives = 130/213 (61%), Gaps = 1/213 (0%) Query: 1 MRLTQEGQLLQLTWLPRLFPVNCYLVEEKEGLTLIDAAMSFSVQGILDTAAKLNKPINRI 60 M++T + Q Q+T LP LFPVNCYLV EK+GLTLID + +GI+ KL P+ RI Sbjct: 1 MKVTAKHQYWQITTLPHLFPVNCYLVLEKDGLTLIDTGILSHAKGIISLITKLKLPLKRI 60 Query: 61 ILTHAHGDHVGALDELKQRLPSAQVYISERDAALLRGDRSLREGEPQTPIKGSVPTKITT 120 +LTH HGDH+G L LK+ P A + + R+ L+ + + E Q P+KGS P + Sbjct: 61 LLTHGHGDHIGGLVALKEAFPEALLMVGSREKLLVE-TKEIYAFEAQKPLKGSYPDQFPV 119 Query: 121 VPDILLQEGDAVGSLTAIATPGHTPGSMSFLDERTGALIAGDALQTFRRTAVAGTVVPFF 180 D LL++GD VGSL + TPGHTPGS+SF DER G L GD QT A+ G F Sbjct: 120 KIDQLLKDGDMVGSLLIMDTPGHTPGSISFFDERNGHLFVGDLFQTRGGAAICGEKRILF 179 Query: 181 PFPAMATWNKASALESAVKLLKLSPKVLAVGHG 213 PFPAM +W+ +++ SA KL + +A GHG Sbjct: 180 PFPAMGSWDLETSIASAEKLQLVDVTEIACGHG 212 >FN1162 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 207 Score = 85.9 bits (211), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 52/156 (33%), Positives = 77/156 (49%), Gaps = 16/156 (10%) Query: 19 FPVNCYLVEEKEGLTLIDAAMSFSVQGILDTAAKLNKPINRIILTHAHGDHVGALDELKQ 78 + NC+L ++ + +++ + + ++ N + I+LTH HGDH+ L++L Sbjct: 11 YGTNCFLAYDENNIAYFFDCGGRNLEKVYEFISEHNLDLKYIVLTHGHGDHIEGLNDLAS 70 Query: 79 RLPSAQVYISERDAALLRGDR-SLRE---GEPQTPIKGSVPTKITTVPDILLQEGDAVGS 134 P A+VYI E D L SL + GE KG + T ++E D VG Sbjct: 71 HYPEAKVYIGEEDKDFLYNSELSLSDAIFGEF-FKFKGEIHT---------VKESDMVGD 120 Query: 135 LTAIATPGHTPGSMSFLDERTGALIAGDALQTFRRT 170 I TPGHT GS SF E+ LI+GD L FRR+ Sbjct: 121 FKVIDTPGHTIGSKSFYYEKDKILISGDTL--FRRS 154 >PH0096 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 198 Score = 79.0 bits (193), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 60/209 (28%), Positives = 90/209 (43%), Gaps = 29/209 (13%) Query: 21 VNCYLVEEKEGLTLIDAAMSFSVQGILDTAAKLNKPINRIILTHAHGDHVGALDELKQRL 80 VN Y+++ + L LID + + + IL +L KP+ +I+TH H DH G+L +K Sbjct: 10 VNVYIIDRGDHLVLIDTGIESTCEKILKKIEELGKPLKTVIITHYHLDHTGSLKCVKDGT 69 Query: 81 PSAQVYISERDAALLRGDRSLREGEPQTPIKGSVPTKITTVPDILLQEGDAVGSLTAIAT 140 + V E A+ E +T ++ VP K +GD V L Sbjct: 70 GAKVVAHEEEVKAI----------EEKTGVRVDVPVK----------DGDVVEGLKVFHM 109 Query: 141 PGHTPGSMSFLDERTGALIAGDALQTFRRTAVAGTVVPFFPFPAMATWNKASALESAVKL 200 PGHT GS+ LDE +GAL GD + R + P + + E +L Sbjct: 110 PGHTRGSICLLDEESGALFVGDLM--VERDG------KLYEVPQQYSEDPMMNRERIKEL 161 Query: 201 LKLSPKVLAVGHGN-LLHAPIEHMKYAVE 228 L + K + HG +L E +K VE Sbjct: 162 LNIEFKAIYPAHGEPVLENAKEKVKELVE 190 >BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 264 Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 63/212 (29%), Positives = 95/212 (44%), Gaps = 21/212 (9%) Query: 22 NCYLVE---EKEGLTLIDAAMSFSVQGILDTAAKL---NKPINRIILTHAHGDHVGALDE 75 N Y ++ E LIDA M S I++ A + + IILTH H DH+GA++E Sbjct: 32 NVYFIQLPSEPHSFVLIDAGMPQSAGVIVNEAKQRFGEGFQLKAIILTHGHFDHIGAIEE 91 Query: 76 LKQRLPSAQVYISERDAALLRGDRSLREGEPQT---------PIKGSVPTKITTVPDILL 126 + + VYI R+ + G P + P+ I++ L Sbjct: 92 ILEHW-DVPVYIHSREMPYVTGKEDYPPARPDSKSGLVAKLSPLFPRHSIDISSHVQALP 150 Query: 127 QEGDA--VGSLTAIATPGHTPGSMSFLDERTGALIAGDALQTFRRTAVAGTVV---PFFP 181 ++G + IATPGHTPG +S + L+AGDA+ T + +A ++ Sbjct: 151 EDGSVPFLDEWMWIATPGHTPGHISLFRDDGRVLVAGDAVITVEQEKMADVLIQKQELHG 210 Query: 182 FPAMATWNKASALESAVKLLKLSPKVLAVGHG 213 PA T + +A ES +KL L P+ L GHG Sbjct: 211 PPAYFTPDTETAAESILKLAGLEPEALLTGHG 242 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,208,555 Number of extensions: 681568 Number of successful extensions: 1869 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 1865 Number of HSP's successfully gapped: 50 Length of query: 239 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 136 Effective length of database: 26,499,029 Effective search space: 3603867944 Effective search space used: 3603867944 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)