BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0049.p (409 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 494 e-139 lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 436 e-122 DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 384 e-106 AGc4702 [S] COG5476 Uncharacterized conserved protein 331 2e-90 AGc4707 [K] COG1737 Transcriptional regulators 331 2e-90 >BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 494 bits (1272), Expect = e-139, Method: Compositional matrix adjust. Identities = 229/409 (55%), Positives = 301/409 (73%) Query: 1 MLSFTQKLENYALLTVKIGINIQPGQTLVVNADIASAELVRLVVRKAYEAGAKLVKVNYT 60 M +F + LE YA L VK+G+NIQPGQTLV+ I++ ELVRLV +KAYEAGAK V V ++ Sbjct: 1 MTTFQENLEKYAELAVKVGVNIQPGQTLVIRTPISALELVRLVAKKAYEAGAKNVHVEWS 60 Query: 61 DELVTRTRYELAPSESFLEPPKWQADELEDLAKNGAAFLSVISTNPDLLNGIDPVRIADN 120 DE +TRT++ LAP E+F E P W A E+LA+NGAAFLS+ +PDLL DP R+A+ Sbjct: 61 DEELTRTKFHLAPDEAFHEYPAWIAKGFEELAENGAAFLSITGQDPDLLKDADPERVANL 120 Query: 121 QRTAGQAMMPYRELLMGNHVSWSLVAYPSPVWAAKVFPDAPPEQQVELLWDAIFKAVRAD 180 + G+A+ +R +M + VSWS+VA PS WA K+ PD ++ VE LW+AIF A R D Sbjct: 121 NKAGGKALKTFRSYIMADKVSWSIVAAPSEGWAKKISPDESKDRAVEKLWEAIFAATRTD 180 Query: 181 QENPVEAWSLHLAGLKQRCDILNAKKYRKLHYTAPGTDLMLELPEGHIWCQAGAVNGRGV 240 Q +PV AW HL L ++ + LN ++Y LHY APGTDL +ELP+ H+W G+ N GV Sbjct: 181 QADPVAAWKNHLKTLNEKMNELNKRRYHALHYKAPGTDLTIELPDTHLWVSGGSTNKDGV 240 Query: 241 PFLANIPTEEVFTAPLKTGANGTVSSTKPLSYSGNIIDRFTLTLENGKVTDFTAEVGQDA 300 F+ANIPTEEVFTA KTG NGTV++TKPL+Y G +ID+FTLT ++GKV DFTAE G + Sbjct: 241 DFVANIPTEEVFTAAKKTGVNGTVTNTKPLNYGGTLIDQFTLTFKDGKVVDFTAEQGYET 300 Query: 301 LASLLAMDEGSAYFGEVALVPYHSPISESGILYFTTLYDENASCHLALGAAYAFTLEQGT 360 L LL DEG+ Y GEVALVP+ SPISE+ ++++ TL+DENAS HLA+G AYAF +E G Sbjct: 301 LKRLLDTDEGARYIGEVALVPHQSPISETDLIFYNTLFDENASNHLAIGTAYAFNIEGGK 360 Query: 361 AMTKEQLAEHGMNQSLTHVDFMMGSADMNIDGITDEGLAEPIFRNGNWA 409 M++E+L + G+N S+THVDFM+GS++M+IDGIT +G EP+FR GNWA Sbjct: 361 EMSEEELEQAGINTSITHVDFMIGSSEMDIDGITKDGKREPVFRKGNWA 409 >lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 436 bits (1121), Expect = e-122, Method: Compositional matrix adjust. Identities = 211/409 (51%), Positives = 284/409 (69%) Query: 1 MLSFTQKLENYALLTVKIGINIQPGQTLVVNADIASAELVRLVVRKAYEAGAKLVKVNYT 60 M F++KLE YA L VK+G+N+QP Q +V+ A + +A LVRL+ + A+E GA+ V +++ Sbjct: 1 MTVFSEKLEKYAELIVKVGVNVQPEQKVVIMAPVDAAPLVRLISKYAFEVGAEDVIMDWR 60 Query: 61 DELVTRTRYELAPSESFLEPPKWQADELEDLAKNGAAFLSVISTNPDLLNGIDPVRIADN 120 DE + RY+ AP F P + E +LAK GA F+S+ S +PDLLNG+D +IA Sbjct: 61 DEELGALRYKNAPLRVFESAPVHRVAEKTELAKEGACFISITSEDPDLLNGVDSNKIATF 120 Query: 121 QRTAGQAMMPYRELLMGNHVSWSLVAYPSPVWAAKVFPDAPPEQQVELLWDAIFKAVRAD 180 Q+T G AM +REL+ N VSW++VA S WAAKVFPD P +Q+E LW+AIF+ R + Sbjct: 121 QKTMGGAMSEFRELMQANVVSWTVVAAASQGWAAKVFPDLTPAEQMETLWEAIFETTRIN 180 Query: 181 QENPVEAWSLHLAGLKQRCDILNAKKYRKLHYTAPGTDLMLELPEGHIWCQAGAVNGRGV 240 ENPVE W H L + + LN K++ LHYTAPGTDL + LP+ H+W AG+ N +G Sbjct: 181 TENPVETWKNHDQTLAAKAESLNEKQFTSLHYTAPGTDLTIGLPKNHLWVGAGSKNKKGH 240 Query: 241 PFLANIPTEEVFTAPLKTGANGTVSSTKPLSYSGNIIDRFTLTLENGKVTDFTAEVGQDA 300 F+AN+PTEEVF K G VSSTKPLSY+GNIID F +T E G++ A G++ Sbjct: 241 EFMANMPTEEVFCCADKLKVEGYVSSTKPLSYAGNIIDDFKITFEKGRIVGVEAASGEEI 300 Query: 301 LASLLAMDEGSAYFGEVALVPYHSPISESGILYFTTLYDENASCHLALGAAYAFTLEQGT 360 L L+A DEGS Y GEVALVP SPIS+SGIL++ TL+DENAS HLA+G+AYAF ++ G Sbjct: 301 LKDLIATDEGSHYLGEVALVPDPSPISQSGILFYNTLFDENASNHLAIGSAYAFNVKGGE 360 Query: 361 AMTKEQLAEHGMNQSLTHVDFMMGSADMNIDGITDEGLAEPIFRNGNWA 409 M++E+L G+N SLTHVDFM+GS++M+IDG+T+ G P+FR G+WA Sbjct: 361 EMSREELEAAGVNNSLTHVDFMIGSSEMDIDGVTESGEVVPVFRKGDWA 409 >DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 417 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/407 (47%), Positives = 269/407 (66%) Query: 2 LSFTQKLENYALLTVKIGINIQPGQTLVVNADIASAELVRLVVRKAYEAGAKLVKVNYTD 61 LSF QKL+NYA L V++G+ ++PGQ ++V + + +A L RL+VR+AY AGA+ V V + D Sbjct: 9 LSFEQKLQNYARLAVRVGLGVKPGQRVLVQSPVDAAPLARLIVREAYAAGARFVDVRWDD 68 Query: 62 ELVTRTRYELAPSESFLEPPKWQADELEDLAKNGAAFLSVISTNPDLLNGIDPVRIADNQ 121 + V R+ELAP +F E +W+ D + A G A +++ +T+P+LL +DP R+A Q Sbjct: 69 DDVQLARFELAPDGTFEEISQWRVDAELETANAGGAVIAIRATDPNLLAKVDPERVATWQ 128 Query: 122 RTAGQAMMPYRELLMGNHVSWSLVAYPSPVWAAKVFPDAPPEQQVELLWDAIFKAVRADQ 181 R PY +M N ++W+LV+ P P WA +FP+A EQ VE WDAIF AVRADQ Sbjct: 129 RANATYRKPYSLQVMTNRLNWNLVSAPIPGWATLMFPEASSEQAVEQQWDAIFAAVRADQ 188 Query: 182 ENPVEAWSLHLAGLKQRCDILNAKKYRKLHYTAPGTDLMLELPEGHIWCQAGAVNGRGVP 241 +PV W HLA LK+R +IL K+Y LH+ TDL + L + HIW A GV Sbjct: 189 PDPVALWEEHLANLKRRREILTGKQYHALHFRGGDTDLTVGLADDHIWGGGAADTPSGVT 248 Query: 242 FLANIPTEEVFTAPLKTGANGTVSSTKPLSYSGNIIDRFTLTLENGKVTDFTAEVGQDAL 301 F ANIPTEEV+TAP + +G V STKPLSY+G +ID + ++G++T +A+ G+ AL Sbjct: 249 FTANIPTEEVWTAPHRERVDGVVVSTKPLSYAGTLIDGIRIEFKDGRITGASAKQGEAAL 308 Query: 302 ASLLAMDEGSAYFGEVALVPYHSPISESGILYFTTLYDENASCHLALGAAYAFTLEQGTA 361 ++ DEGS GEVALVP+ SPIS SG+ ++ TLYDENA+ H+A+G+AY F ++ G Sbjct: 309 LKMIDTDEGSHRLGEVALVPHSSPISRSGLFFYNTLYDENAASHIAIGSAYRFNVKGGID 368 Query: 362 MTKEQLAEHGMNQSLTHVDFMMGSADMNIDGITDEGLAEPIFRNGNW 408 M EQ A+ G N SLTHVD+M+GS +++DGIT +G EP+ R G + Sbjct: 369 MDAEQFAQKGGNDSLTHVDWMVGSDQIDVDGITKDGNREPVMRAGEF 415 >AGc4702 [S] COG5476 Uncharacterized conserved protein Length = 412 Score = 331 bits (848), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 166/403 (41%), Positives = 239/403 (59%) Query: 7 KLENYALLTVKIGINIQPGQTLVVNADIASAELVRLVVRKAYEAGAKLVKVNYTDELVTR 66 KLE A + +K+G+ +Q Q LV+ A +A+ LVRL+ + AY AG LV Y+DE T Sbjct: 10 KLEKLAEVAIKVGLQLQKDQDLVITAPLAALPLVRLLTKHAYIAGGGLVTTFYSDEETTL 69 Query: 67 TRYELAPSESFLEPPKWQADELEDLAKNGAAFLSVISTNPDLLNGIDPVRIADNQRTAGQ 126 +RY A +F W + + NGAA L++ NP LL DP ++A + Sbjct: 70 SRYRHASDANFDRASGWLYEGMAKAYANGAARLAIAGDNPMLLAEEDPAKVARANKANST 129 Query: 127 AMMPYRELLMGNHVSWSLVAYPSPVWAAKVFPDAPPEQQVELLWDAIFKAVRADQENPVE 186 A P E + ++W++++YP+P WA +VFP ++ V L DAIF A R D +PV Sbjct: 130 AYKPALEKISNFDINWNIISYPNPSWAKQVFPGLTEDEAVRKLADAIFAASRVDVADPVA 189 Query: 187 AWSLHLAGLKQRCDILNAKKYRKLHYTAPGTDLMLELPEGHIWCQAGAVNGRGVPFLANI 246 AW+ H A L +R LN +++ LH+T PGTD+ + L +GH W ++ GV NI Sbjct: 190 AWAQHNANLAKRSAWLNGERFSALHFTGPGTDVKIGLADGHEWHGGASMAKNGVTCNPNI 249 Query: 247 PTEEVFTAPLKTGANGTVSSTKPLSYSGNIIDRFTLTLENGKVTDFTAEVGQDALASLLA 306 PTEEVFT P +G VSSTKPLS+ G +ID + E G++ + A G+ L +L Sbjct: 250 PTEEVFTTPHALRVDGYVSSTKPLSHQGTLIDDIQVKFEAGRIVEAKASKGEAVLNKVLD 309 Query: 307 MDEGSAYFGEVALVPYHSPISESGILYFTTLYDENASCHLALGAAYAFTLEQGTAMTKEQ 366 DEG+ GEVALVP+ SPIS SGIL++ TL+DENASCH+ALG Y+ G +++++Q Sbjct: 310 TDEGARRLGEVALVPHSSPISASGILFYNTLFDENASCHIALGQCYSKCFLDGASLSQDQ 369 Query: 367 LAEHGMNQSLTHVDFMMGSADMNIDGITDEGLAEPIFRNGNWA 409 + G N SL H+D+M+GS ++IDG+ +G P+ R G WA Sbjct: 370 IKAQGGNSSLIHIDWMIGSNKVDIDGVKPDGSTVPVMRKGEWA 412 >AGc4707 [K] COG1737 Transcriptional regulators Length = 412 Score = 331 bits (848), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 166/403 (41%), Positives = 239/403 (59%) Query: 7 KLENYALLTVKIGINIQPGQTLVVNADIASAELVRLVVRKAYEAGAKLVKVNYTDELVTR 66 KLE A + +K+G+ +Q Q LV+ A +A+ LVRL+ + AY AG LV Y+DE T Sbjct: 10 KLEKLAEVAIKVGLQLQKDQDLVITAPLAALPLVRLLTKHAYIAGGGLVTTFYSDEETTL 69 Query: 67 TRYELAPSESFLEPPKWQADELEDLAKNGAAFLSVISTNPDLLNGIDPVRIADNQRTAGQ 126 +RY A +F W + + NGAA L++ NP LL DP ++A + Sbjct: 70 SRYRHASDANFDRASGWLYEGMAKAYANGAARLAIAGDNPMLLAEEDPAKVARANKANST 129 Query: 127 AMMPYRELLMGNHVSWSLVAYPSPVWAAKVFPDAPPEQQVELLWDAIFKAVRADQENPVE 186 A P E + ++W++++YP+P WA +VFP ++ V L DAIF A R D +PV Sbjct: 130 AYKPALEKISNFDINWNIISYPNPSWAKQVFPGLTEDEAVRKLADAIFAASRVDVADPVA 189 Query: 187 AWSLHLAGLKQRCDILNAKKYRKLHYTAPGTDLMLELPEGHIWCQAGAVNGRGVPFLANI 246 AW+ H A L +R LN +++ LH+T PGTD+ + L +GH W ++ GV NI Sbjct: 190 AWAQHNANLAKRSAWLNGERFSALHFTGPGTDVKIGLADGHEWHGGASMAKNGVTCNPNI 249 Query: 247 PTEEVFTAPLKTGANGTVSSTKPLSYSGNIIDRFTLTLENGKVTDFTAEVGQDALASLLA 306 PTEEVFT P +G VSSTKPLS+ G +ID + E G++ + A G+ L +L Sbjct: 250 PTEEVFTTPHALRVDGYVSSTKPLSHQGTLIDDIQVKFEAGRIVEAKASKGEAVLNKVLD 309 Query: 307 MDEGSAYFGEVALVPYHSPISESGILYFTTLYDENASCHLALGAAYAFTLEQGTAMTKEQ 366 DEG+ GEVALVP+ SPIS SGIL++ TL+DENASCH+ALG Y+ G +++++Q Sbjct: 310 TDEGARRLGEVALVPHSSPISASGILFYNTLFDENASCHIALGQCYSKCFLDGASLSQDQ 369 Query: 367 LAEHGMNQSLTHVDFMMGSADMNIDGITDEGLAEPIFRNGNWA 409 + G N SL H+D+M+GS ++IDG+ +G P+ R G WA Sbjct: 370 IKAQGGNSSLIHIDWMIGSNKVDIDGVKPDGSTVPVMRKGEWA 412 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,285,522 Number of extensions: 1338388 Number of successful extensions: 2251 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2241 Number of HSP's successfully gapped: 12 Length of query: 409 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 300 Effective length of database: 25,807,025 Effective search space: 7742107500 Effective search space used: 7742107500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)