BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0048.p (412 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 521 e-148 lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 461 e-129 DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 350 3e-96 AGc4702 [S] COG5476 Uncharacterized conserved protein 325 1e-88 AGc4707 [K] COG1737 Transcriptional regulators 325 1e-88 >BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 521 bits (1342), Expect = e-148, Method: Compositional matrix adjust. Identities = 251/412 (60%), Positives = 303/412 (73%), Gaps = 2/412 (0%) Query: 1 MPQLQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWX 60 M Q L KYA+LAV++GVN+QPGQTL++ PISA E VRL+ KAY GA V V W Sbjct: 1 MTTFQENLEKYAELAVKVGVNIQPGQTLVIRTPISALELVRLVAKKAYEAGAKNVHVEWS 60 Query: 61 XXXEFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIA 120 E +TR F A E F + P W A E AENGAAFL + +DPD LK D ER+A Sbjct: 61 D--EELTRTKFHLAPDEAFHEYPAWIAKGFEELAENGAAFLSITGQDPDLLKDADPERVA 118 Query: 121 NYQKTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVR 180 N K G+AL +R M+DKVSWSIVA PS+ WA K+ PD D V+KLWEAIF R Sbjct: 119 NLNKAGGKALKTFRSYIMADKVSWSIVAAPSEGWAKKISPDESKDRAVEKLWEAIFAATR 178 Query: 181 LDREDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAK 240 D+ DPVAAW+ HL TL +K N LN ++Y +LHY APGTDL+IELP+ HLW G S N Sbjct: 179 TDQADPVAAWKNHLKTLNEKMNELNKRRYHALHYKAPGTDLTIELPDTHLWVSGGSTNKD 238 Query: 241 GHSFVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQ 300 G FVAN+PTEEVFTA KTGVNGTV TKPL+YGG +ID F++TF+ G++V AEQG Sbjct: 239 GVDFVANIPTEEVFTAAKKTGVNGTVTNTKPLNYGGTLIDQFTLTFKDGKVVDFTAEQGY 298 Query: 301 EALEYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEG 360 E L+ L+ DEGA+Y+GEVALVPH+SPISE++++++NTLFDENASNHLAIGTAYAF +EG Sbjct: 299 ETLKRLLDTDEGARYIGEVALVPHQSPISETDLIFYNTLFDENASNHLAIGTAYAFNIEG 358 Query: 361 GKEMNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 GKEM+++EL G+NTS+THVDFMIGS+EMDI GIT DG REPVF KGNWAF Sbjct: 359 GKEMSEEELEQAGINTSITHVDFMIGSSEMDIDGITKDGKREPVFRKGNWAF 410 >lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 461 bits (1186), Expect = e-129, Method: Compositional matrix adjust. Identities = 226/412 (54%), Positives = 291/412 (70%), Gaps = 2/412 (0%) Query: 1 MPQLQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWX 60 M +L KYA+L V++GVNVQP Q +++ AP+ AA VRLI+ A+ +GA V ++W Sbjct: 1 MTVFSEKLEKYAELIVKVGVNVQPEQKVVIMAPVDAAPLVRLISKYAFEVGAEDVIMDWR 60 Query: 61 XXXEFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIA 120 E + +++A VF P E TE A+ GA F+ + +EDPD L G+DS +IA Sbjct: 61 D--EELGALRYKNAPLRVFESAPVHRVAEKTELAKEGACFISITSEDPDLLNGVDSNKIA 118 Query: 121 NYQKTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVR 180 +QKT G A++++REL ++ VSW++VA SQ WA KVFPD+ E+++ LWEAIF T R Sbjct: 119 TFQKTMGGAMSEFRELMQANVVSWTVVAAASQGWAAKVFPDLTPAEQMETLWEAIFETTR 178 Query: 181 LDREDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAK 240 ++ E+PV W+ H TL KA LN K++ SLHY APGTDL+I LP+ HLW S N K Sbjct: 179 INTENPVETWKNHDQTLAAKAESLNEKQFTSLHYTAPGTDLTIGLPKNHLWVGAGSKNKK 238 Query: 241 GHSFVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQ 300 GH F+ANMPTEEVF K V G V +TKPLSY GNIID F ITFE+GRIV V A G+ Sbjct: 239 GHEFMANMPTEEVFCCADKLKVEGYVSSTKPLSYAGNIIDDFKITFEKGRIVGVEAASGE 298 Query: 301 EALEYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEG 360 E L+ LI DEG+ YLGEVALVP SPIS+S IL++NTLFDENASNHLAIG+AYAF ++G Sbjct: 299 EILKDLIATDEGSHYLGEVALVPDPSPISQSGILFYNTLFDENASNHLAIGSAYAFNVKG 358 Query: 361 GKEMNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 G+EM+++EL A G+N S+THVDFMIGS+EMDI G+T G PVF KG+WAF Sbjct: 359 GEEMSREELEAAGVNNSLTHVDFMIGSSEMDIDGVTESGEVVPVFRKGDWAF 410 >DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 417 Score = 350 bits (897), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 175/405 (43%), Positives = 257/405 (63%), Gaps = 2/405 (0%) Query: 4 LQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXX 63 + +L YA LAVR+G+ V+PGQ ++V +P+ AA RLI +AYA GA V V W Sbjct: 11 FEQKLQNYARLAVRVGLGVKPGQRVLVQSPVDAAPLARLIVREAYAAGARFVDVRWDDDD 70 Query: 64 EFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQ 123 + R FE A F + W E A G A + + A DP+ L +D ER+A +Q Sbjct: 71 VQLAR--FELAPDGTFEEISQWRVDAELETANAGGAVIAIRATDPNLLAKVDPERVATWQ 128 Query: 124 KTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDR 183 + Y M+++++W++V+ P WA +FP+ +++ V++ W+AIF VR D+ Sbjct: 129 RANATYRKPYSLQVMTNRLNWNLVSAPIPGWATLMFPEASSEQAVEQQWDAIFAAVRADQ 188 Query: 184 EDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHS 243 DPVA W+EHL L+++ +L K+Y +LH+ TDL++ L + H+W G + G + Sbjct: 189 PDPVALWEEHLANLKRRREILTGKQYHALHFRGGDTDLTVGLADDHIWGGGAADTPSGVT 248 Query: 244 FVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEAL 303 F AN+PTEEV+TAP + V+G V +TKPLSY G +IDG I F+ GRI +A+QG+ AL Sbjct: 249 FTANIPTEEVWTAPHRERVDGVVVSTKPLSYAGTLIDGIRIEFKDGRITGASAKQGEAAL 308 Query: 304 EYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKE 363 +I DEG+ LGEVALVPH SPIS S + ++NTL+DENA++H+AIG+AY F ++GG + Sbjct: 309 LKMIDTDEGSHRLGEVALVPHSSPISRSGLFFYNTLYDENAASHIAIGSAYRFNVKGGID 368 Query: 364 MNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKG 408 M+ ++ G N S+THVD+M+GS ++D+ GIT DG REPV G Sbjct: 369 MDAEQFAQKGGNDSLTHVDWMVGSDQIDVDGITKDGNREPVMRAG 413 >AGc4702 [S] COG5476 Uncharacterized conserved protein Length = 412 Score = 325 bits (832), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 164/405 (40%), Positives = 245/405 (60%), Gaps = 2/405 (0%) Query: 7 QLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXXEFI 66 +L K A++A+++G+ +Q Q L+++AP++A VRL+T AY G V + + Sbjct: 10 KLEKLAEVAIKVGLQLQKDQDLVITAPLAALPLVRLLTKHAYIAGGGLVTTFYSDEETTL 69 Query: 67 TRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQKTR 126 +R + HA+ F + W + + NGAA L + ++P L D ++A K Sbjct: 70 SR--YRHASDANFDRASGWLYEGMAKAYANGAARLAIAGDNPMLLAEEDPAKVARANKAN 127 Query: 127 GEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDREDP 186 A E + ++W+I++ P+ +WA +VFP + DE V KL +AIF R+D DP Sbjct: 128 STAYKPALEKISNFDINWNIISYPNPSWAKQVFPGLTEDEAVRKLADAIFAASRVDVADP 187 Query: 187 VAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHSFVA 246 VAAW +H L +++ LN +++ +LH+ PGTD+ I L +GH W G S+ G + Sbjct: 188 VAAWAQHNANLAKRSAWLNGERFSALHFTGPGTDVKIGLADGHEWHGGASMAKNGVTCNP 247 Query: 247 NMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEALEYL 306 N+PTEEVFT P V+G V +TKPLS+ G +ID + FE GRIV A +G+ L + Sbjct: 248 NIPTEEVFTTPHALRVDGYVSSTKPLSHQGTLIDDIQVKFEAGRIVEAKASKGEAVLNKV 307 Query: 307 IGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKEMNK 366 + DEGA+ LGEVALVPH SPIS S IL++NTLFDENAS H+A+G Y+ C G +++ Sbjct: 308 LDTDEGARRLGEVALVPHSSPISASGILFYNTLFDENASCHIALGQCYSKCFLDGASLSQ 367 Query: 367 DELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWA 411 D++ A G N+S+ H+D+MIGS ++DI G+ DG+ PV KG WA Sbjct: 368 DQIKAQGGNSSLIHIDWMIGSNKVDIDGVKPDGSTVPVMRKGEWA 412 >AGc4707 [K] COG1737 Transcriptional regulators Length = 412 Score = 325 bits (832), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 164/405 (40%), Positives = 245/405 (60%), Gaps = 2/405 (0%) Query: 7 QLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXXEFI 66 +L K A++A+++G+ +Q Q L+++AP++A VRL+T AY G V + + Sbjct: 10 KLEKLAEVAIKVGLQLQKDQDLVITAPLAALPLVRLLTKHAYIAGGGLVTTFYSDEETTL 69 Query: 67 TRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQKTR 126 +R + HA+ F + W + + NGAA L + ++P L D ++A K Sbjct: 70 SR--YRHASDANFDRASGWLYEGMAKAYANGAARLAIAGDNPMLLAEEDPAKVARANKAN 127 Query: 127 GEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDREDP 186 A E + ++W+I++ P+ +WA +VFP + DE V KL +AIF R+D DP Sbjct: 128 STAYKPALEKISNFDINWNIISYPNPSWAKQVFPGLTEDEAVRKLADAIFAASRVDVADP 187 Query: 187 VAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHSFVA 246 VAAW +H L +++ LN +++ +LH+ PGTD+ I L +GH W G S+ G + Sbjct: 188 VAAWAQHNANLAKRSAWLNGERFSALHFTGPGTDVKIGLADGHEWHGGASMAKNGVTCNP 247 Query: 247 NMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEALEYL 306 N+PTEEVFT P V+G V +TKPLS+ G +ID + FE GRIV A +G+ L + Sbjct: 248 NIPTEEVFTTPHALRVDGYVSSTKPLSHQGTLIDDIQVKFEAGRIVEAKASKGEAVLNKV 307 Query: 307 IGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKEMNK 366 + DEGA+ LGEVALVPH SPIS S IL++NTLFDENAS H+A+G Y+ C G +++ Sbjct: 308 LDTDEGARRLGEVALVPHSSPISASGILFYNTLFDENASCHIALGQCYSKCFLDGASLSQ 367 Query: 367 DELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWA 411 D++ A G N+S+ H+D+MIGS ++DI G+ DG+ PV KG WA Sbjct: 368 DQIKAQGGNSSLIHIDWMIGSNKVDIDGVKPDGSTVPVMRKGEWA 412 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,259,821 Number of extensions: 1353368 Number of successful extensions: 3934 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 3920 Number of HSP's successfully gapped: 12 Length of query: 412 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 303 Effective length of database: 25,807,025 Effective search space: 7819528575 Effective search space used: 7819528575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)