BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0046.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yubG [P] COG0168 Trk-type K+ transport systems, membrane comp... 317 2e-86 BH0598 [P] COG0168 Trk-type K+ transport systems, membrane compo... 306 6e-83 DR1668 [P] COG0168 Trk-type K+ transport systems, membrane compo... 281 1e-75 SA1815 [P] COG0168 Trk-type K+ transport systems, membrane compo... 268 2e-71 BS_ykrM [P] COG0168 Trk-type K+ transport systems, membrane comp... 265 1e-70 >BS_yubG [P] COG0168 Trk-type K+ transport systems, membrane components Length = 445 Score = 317 bits (812), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 176/426 (41%), Positives = 260/426 (61%), Gaps = 10/426 (2%) Query: 4 IAAGTILLCLPAASTGARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXXXXX 63 I G +LL LP ++T +S+IDALF A SAT VTGLAV+DTGTQ TVFGQ Sbjct: 28 IIIGAVLLMLPISTT-KPLSWIDALFTAASATTVTGLAVVDTGTQFTVFGQTVIMGLIQI 86 Query: 64 XXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLSGAI 123 T A LI ++L K+I LKER+L+QE++NQ ++ G++ L++ + ++S+ I+L A+ Sbjct: 87 GGLGFMTFAVLIVMILGKKIGLKERMLVQEALNQPTIGGVIGLVKVLFLFSISIELIAAL 146 Query: 124 LLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNITSML 183 +L+ R + ++ LFH+IS FNNAGF L+ D L Y DP VN+ Sbjct: 147 ILSIRLVPQYGWSSGLFASLFHAISAFNNAGFSLWPD------NLMSYVGDPTVNLVITF 200 Query: 184 LIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATLKPL 241 L GGIGF VL D++ + K +LH+K++L+ + +L F LE + TL L Sbjct: 201 LFITGGIGFTVLFDVMKNRRFKTFSLHTKLMLTGTLMLNAIAMLTVFILEYSNPGTLGHL 260 Query: 242 HAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKITTFA 301 H K+ AS+ QA+TPR+ G +++ +RE T +LLMFIGA ST GIK+TTF Sbjct: 261 HIVDKLWASYFQAVTPRTAGFNSLDFGSMREGTIVFTLLLMFIGAGSASTASGIKLTTFI 320 Query: 302 ILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESADFLTV 361 +++ + +RGK++ V+FR I + +A+ +++ SL +V L++TE A FL + Sbjct: 321 VILTSVIAYLRGKKETVIFRRSIKYPIIIKALAVSVTSLFIVFLGIFALTITEQAPFLQI 380 Query: 362 LFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELYRYPE 421 +FE SAFGT G+TMGLTP LTT GK ++I++MF+GR GPLT ++ K + RYP+ Sbjct: 381 VFETFSAFGTVGLTMGLTPELTTAGKCIIIVIMFIGRIGPLTFVFSFA-KTEQSNIRYPD 439 Query: 422 GNITIG 427 G + G Sbjct: 440 GEVFTG 445 >BH0598 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 448 Score = 306 bits (783), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 176/430 (40%), Positives = 268/430 (62%), Gaps = 12/430 (2%) Query: 1 MVLIAAGTILLCLPAASTGARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXX 60 +V I GT+LL LP AST IS++DA+F ATSA VTGL V+DTGT TVFGQ+ Sbjct: 28 LVFIMIGTLLLKLPIAST-TYISWVDAMFTATSAITVTGLVVVDTGTDYTVFGQVVIMIL 86 Query: 61 XXXXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLS 120 T A L ++L K+I L++R+L+QE+MNQ S+ G++RL++ +L+++LV++ Sbjct: 87 IQLGGLGLMTFAVLAVMMLGKKIGLQQRILIQEAMNQTSLGGLIRLVRVLLLFTLVMETI 146 Query: 121 GAILLAARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNIT 180 ILL+ R+ + + + +Y LFH+IS FNNAGF L+ D L+ Y DP +N+ Sbjct: 147 AFILLSIRWVPEFGWREGMYQSLFHTISAFNNAGFSLWSD------SLSAYVGDPAINLI 200 Query: 181 SMLLIFLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATL 238 +L GGIGF VL+DL + L+LHSK++L + L F LE TL Sbjct: 201 ITILFITGGIGFTVLADLWYKKTFRALSLHSKIMLLGTLALNCIAFIAIFILEYANPHTL 260 Query: 239 KPLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKIT 298 L + ++ QA+TPR+ G T+EI + ++ L++LLMFIGA ST GIK+T Sbjct: 261 GGLAQSDRFWGAYFQAVTPRTAGFNTLEIADMTTASIVLILLLMFIGAGSASTASGIKVT 320 Query: 299 TFAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESADF 358 TF ++++ ++ ++G+ + V+F I + RA+ + ++S+M+V A +L++TE A F Sbjct: 321 TFFVMILATWSFLKGRAETVVFERTIKLHTILRALAIIVISMMIVFIAVFILAITEDAPF 380 Query: 359 LTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYAL-KPKNSKELY 417 + +FE SAFGT G++MGLT LTT+GK+ ++++MF+GR GPLT+A+ L +PK S Sbjct: 381 IVKVFEVFSAFGTVGLSMGLTGELTTLGKMTIMVVMFIGRVGPLTMAFTLARPKQSN--I 438 Query: 418 RYPEGNITIG 427 RYPE + G Sbjct: 439 RYPEDEVFTG 448 >DR1668 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 512 Score = 281 bits (720), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 174/432 (40%), Positives = 258/432 (59%), Gaps = 17/432 (3%) Query: 7 GTILLCLP-AASTGARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXXXXXXX 65 G +LL LP A G I+FI ALFMATS CVTGLAV+D G+ VFG+ Sbjct: 87 GAVLLWLPFAHEAGKSITFIQALFMATSCVCVTGLAVVDPGSTFNVFGESVMLLLIQVGG 146 Query: 66 XXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLSGAILL 125 T+ TL L L +R+ + R+ Q+ ++ S+ L++ +++ SL+ + GA+LL Sbjct: 147 LGIITLGTLFALALRRRLGVIGRINAQQQVSATSLGSATSLVRSIVLTSLLFEGLGALLL 206 Query: 126 AARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNITSMLLI 185 F + +YY +FHSIS FNNAGF L+ + LT + DP++N+ LLI Sbjct: 207 LPAFIPREGVLRGIYYAIFHSISSFNNAGFSLYS------TNLTGFVSDPLINMVVTLLI 260 Query: 186 FLGGIGFIVLSDLIDY---PKRKRLTLHSKVVLSASAVLILGGAAVFFWLELN--ATLKP 240 GG+GF+V +L+ + +R RL+ ++++ L+ + VL++ G F E N TL P Sbjct: 261 IFGGMGFLVQLNLLGWLRDRRRNRLSTNTRLSLTMTGVLLVVGTVFFALTEWNNPGTLAP 320 Query: 241 LHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKITTF 300 L GK++AS+ Q++TPR+ G T++ L+ F+ I+ MFIGA PGSTGGGIK TF Sbjct: 321 LPLHGKLLASWFQSVTPRTAGFNTLDYGLMGFPALFMTIVWMFIGANPGSTGGGIKTNTF 380 Query: 301 AILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATM---LLSVTESAD 357 ++V +A++ +RG+ D +F RI E + RA+T+ LLS+ LV + M LL+ + Sbjct: 381 YVIVASAWSMVRGRRDTTLFDRRIDTETLLRAMTVGLLSMGLVTAGFMLMLLLNTNKKVL 440 Query: 358 FLTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYAL-KPKNSKEL 416 FL + FE VSAFGT+G++M TP L T + ++I LMF+GR GPLT A A +P + K L Sbjct: 441 FLQLFFETVSAFGTAGLSMNTTPLLNTAQEAVLIALMFLGRIGPLTFAVAFSRPASEKML 500 Query: 417 YRYP-EGNITIG 427 RYP + +I IG Sbjct: 501 TRYPADRDILIG 512 >SA1815 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 435 Score = 268 bits (684), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 165/420 (39%), Positives = 246/420 (58%), Gaps = 10/420 (2%) Query: 7 GTILLCLPAASTGAR-ISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXXXXXXX 65 G +LL LP TG + ISF+DALF+A+SA VTGL+ +D G+Q + G+I Sbjct: 22 GALLLYLPF--TGKKPISFLDALFIASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGG 79 Query: 66 XXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLSGAILL 125 T+ L + LN++IS+K R L+ + N + G+++LI+ + IYSLV +L G I L Sbjct: 80 LGIVTVTLLTLVFLNRKISMKNRFLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICL 139 Query: 126 AARFTVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNITSMLLI 185 F GK ++ LF S+S FNNAGF LF + L Y+ DPIV IT +LI Sbjct: 140 CLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKN------NLIDYSSDPIVIITISILI 193 Query: 186 FLGGIGFIVLSDLIDYPKRKRLTLHSKVVLSASAVLILGGAAVFFWLELNATLKPLHAGG 245 GGIG V+ D I+ K +L+LHSK+VL+ +++LI+ GA FF LE T++ + Sbjct: 194 IFGGIGHFVVIDFINCKKLSKLSLHSKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVE 253 Query: 246 KVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKITTFAILVV 305 K+ SF Q++T R+ G +I+I + +ST +++LLMFIG AP S GGIKITTFA+ + Sbjct: 254 KIGNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFI 313 Query: 306 TAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLS-VTESADFLTVLFE 364 IR + ++ +F IS +++ +I +S + + T +LS + + + +LFE Sbjct: 314 FVLNYIRKENNVSVFNKEISDKHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFE 373 Query: 365 AVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELYRYPEGNI 424 VSAFGT G++M LT I K+++I +M G+ G LTL P S + YRY +G I Sbjct: 374 VVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQI 433 >BS_ykrM [P] COG0168 Trk-type K+ transport systems, membrane components Length = 449 Score = 265 bits (676), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 160/430 (37%), Positives = 240/430 (55%), Gaps = 12/430 (2%) Query: 4 IAAGTILLCLPAAST-GARISFIDALFMATSATCVTGLAVIDTGTQLTVFGQIXXXXXXX 62 + ILL LPAA GA +FIDALF A S+ VTGL V+DT + G Sbjct: 26 VTVAVILLSLPAAHKPGADWTFIDALFTAVSSVSVTGLTVVDTADTFSTIGIFILAFVLQ 85 Query: 63 XXXXXXXTMATLITLVLNKRISLKERLLLQESMNQNSMQGIVRLIQRVLIYSLVIQLSGA 122 T+ T I L++ KRI LKER L+ NQ+ GIV L+++VL L I+ G Sbjct: 86 FGGIGIMTLGTFIWLIMGKRIGLKERKLIMVDQNQSQFSGIVNLMKQVLFLILWIEFFGG 145 Query: 123 ILLAARF-TVDMSFGKAVYYGLFHSISIFNNAGFDLFGDIHGPFSGLTRYAEDPIVNITS 181 ++L F T S+ +A +G F SIS N GFD+ G+ PF D V + Sbjct: 146 LILGTYFLTYYDSYQEAYLHGFFASISATTNGGFDITGNSMIPFR------HDYFVQFIT 199 Query: 182 MLLIFLGGIGFIVLSDLIDY----PKRKRLTLHSKVVLSASAVLILGGAAVFFWLELNAT 237 MLLI G IGF VL ++ D+ +R TL +K+ L+L GA F LE N Sbjct: 200 MLLIIFGAIGFPVLVEVKDFLFSKHRRYPFTLFTKITTITFGSLVLFGAIGIFALEANHA 259 Query: 238 LKPLHAGGKVMASFLQAITPRSGGVTTIEIPLLRESTQFLMILLMFIGAAPGSTGGGIKI 297 + S Q+ RSGG+ TI+I L +ST F + LMFIGA+P S GGGI+ Sbjct: 260 FAGKSWHDILFLSLFQSTATRSGGLATIDISQLSDSTLFFICALMFIGASPSSVGGGIRT 319 Query: 298 TTFAILVVTAYTKIRGKEDIVMFRHRISKENVYRAITMTLLSLMLVVSATMLLSVTESAD 357 TTFA+ ++ + RG + + +F+ + ++ +++ +T+++++LV AT++L++TE Sbjct: 320 TTFALNLLALFHFARGNKAVKVFKRELHPADLMKSLVVTMMAILLVFGATLILTITEKHS 379 Query: 358 FLTVLFEAVSAFGTSGITMGLTPNLTTIGKVLVIILMFVGRTGPLTLAYALKPKNSKELY 417 L +LFE SAFGT+G+++G+T +L+++GK +++I+MF+GR G LT Y + K + Y Sbjct: 380 LLELLFEVCSAFGTTGLSLGITADLSSVGKCVIMIVMFIGRIGILTFLYLIGRKEIEANY 439 Query: 418 RYPEGNITIG 427 YP+ + IG Sbjct: 440 HYPKERVIIG 449 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,762,819 Number of extensions: 998310 Number of successful extensions: 3130 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3080 Number of HSP's successfully gapped: 17 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)