BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0042.p (457 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yrkA [R] COG1253 Hemolysins and related proteins containing C... 392 e-109 BS_yhdP [R] COG1253 Hemolysins and related proteins containing C... 378 e-105 BS_yhdT [R] COG1253 Hemolysins and related proteins containing C... 363 e-100 lin2334 [R] COG1253 Hemolysins and related proteins containing C... 362 e-100 BS_yugS [R] COG1253 Hemolysins and related proteins containing C... 353 3e-97 >BS_yrkA [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 392 bits (1006), Expect = e-109, Method: Compositional matrix adjust. Identities = 204/408 (50%), Positives = 272/408 (66%), Gaps = 8/408 (1%) Query: 34 FFVATEFALVRLRGSQISQMVLEGKKNALAVQRVAANLDGYLSACQLGITITALGIGALA 93 FFVATEFA+V++R S+I Q++LEGKK A++ ++V +LD YLSACQLGIT+TALGIG + Sbjct: 18 FFVATEFAIVKIRSSKIDQLILEGKKGAISAKKVITHLDEYLSACQLGITVTALGIGWVG 77 Query: 94 EPAFEQLLIPLFDLGNISHSVSEPXXXXXXXXXXXXXHVVVGELAPKTAAINIPEKIGQI 153 E FE +L PLF ++S +VS HVVVGELAPKT AI E I + Sbjct: 78 ESTFEVILHPLFAHFHVSETVSHVLILVIAFVMATFLHVVVGELAPKTLAIQKAETITLL 137 Query: 154 TSPLIIWFYKILYPLIWLMNGSANLLVRMFGMKPASEHGDAHSEDEIRLILSESYESGKI 213 T+ IIWFY+IL+P IW +NGSA +V +FG+KPASEH AHSE+E+R++LSESY+SG+I Sbjct: 138 TAKPIIWFYRILFPFIWFLNGSARFIVGLFGLKPASEHELAHSEEELRILLSESYKSGEI 197 Query: 214 NKAEYGYVNRIFTFDEMLAKEIMVPRTDMVCLFTNHSLKENLEIIRKEQYTRFPVADGSK 273 N+ E YVN IF FDE +AKEIM+PR ++V + + S + ++II+ E YTR+PV +G K Sbjct: 198 NQNELKYVNNIFEFDERIAKEIMIPRREIVAISSEDSYETIVKIIKTESYTRYPVLNGDK 257 Query: 274 DNIIGMINTKQL---YLQYDN--NPDFDFKSLILPLLTVSEVTPXXXXTLLTRMQKERVH 328 D+IIG IN K+ Y+ D DF ++ I P++ V E P +L +MQKER H Sbjct: 258 DSIIGFINAKEFLSAYIDTDQKIKEDFKLENHINPVIHVIESVP--IHDVLVKMQKERTH 315 Query: 329 IALLLDEYGGTSGLITIEDILEEIVGEIXXXXXXXXXXNIEKLSDSHYLFDGNVSVLEVK 388 IA+L+DEYGGTSGL+T EDILEEIVGEI NI K++D+HY+ D V + +V Sbjct: 316 IAILVDEYGGTSGLVTAEDILEEIVGEIRDEFDKDEVPNIRKVNDNHYILDSKVLIEDVN 375 Query: 389 ELTGFDLHDDEVTTIGGWLYSHLEEPAIGKSITHENVTLTVRE-MNRH 435 +L G L DEV TIGGW + + A+G I + V E + RH Sbjct: 376 DLLGTTLASDEVDTIGGWFMTQQIDAAVGSVIEADGYIFKVHETVGRH 423 >BS_yhdP [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 444 Score = 378 bits (971), Expect = e-105, Method: Compositional matrix adjust. Identities = 192/416 (46%), Positives = 274/416 (65%), Gaps = 5/416 (1%) Query: 34 FFVATEFALVRLRGSQISQMVLEGKKNALAVQRVAANLDGYLSACQLGITITALGIGALA 93 FFVA+EFA++R+RGS+I Q++ EG K A+AV++V +LD YLSACQLGIT+T++G+G L Sbjct: 18 FFVASEFAIIRIRGSRIDQLIAEGNKAAIAVKKVTTHLDEYLSACQLGITLTSIGLGVLG 77 Query: 94 EPAFEQLLIPLFDLGNISHSVSEPXXXXXXXXXXXXXHVVVGELAPKTAAINIPEKIGQI 153 E E+LL PLF N+ S+S HVVVGELAPKT AI E + + Sbjct: 78 ESTIERLLHPLFVQMNVPGSLSHVISFIFAYAIITFLHVVVGELAPKTVAIQKAEAVSML 137 Query: 154 TSPLIIWFYKILYPLIWLMNGSANLLVRMFGMKPASEHGDAHSEDEIRLILSESYESGKI 213 + +IWFY+I +P IWL+N SA LL + FG++ SE+ AHSE+E+R+ILSESY+SG+I Sbjct: 138 FAKPLIWFYRIAFPFIWLLNNSARLLTKAFGLETVSENELAHSEEELRIILSESYKSGEI 197 Query: 214 NKAEYGYVNRIFTFDEMLAKEIMVPRTDMVCLFTNHSLKENLEIIRKEQYTRFPVADGSK 273 N++E+ YVN+IF FD+ LAKEIM+PRT++V L + + E ++II+ E+YTR+PV +G K Sbjct: 198 NQSEFKYVNKIFEFDDRLAKEIMIPRTEIVSLPHDIKISEMMDIIQIEKYTRYPVEEGDK 257 Query: 274 DNIIGMINTKQLY---LQYDNNPDFDFKSLILPLLTVSEVTPXXXXTLLTRMQKERVHIA 330 DNIIG+IN K++ + + + D + P++ V E P LL +MQKERVH+A Sbjct: 258 DNIIGVINIKEVLTACISGEVSVDSTISQFVNPIIHVIESAP--IQDLLVKMQKERVHMA 315 Query: 331 LLLDEYGGTSGLITIEDILEEIVGEIXXXXXXXXXXNIEKLSDSHYLFDGNVSVLEVKEL 390 +L DEYGGT+GL+T+EDI+EEIVGEI I K+ + HY+ DG V + +V +L Sbjct: 316 ILSDEYGGTAGLVTVEDIIEEIVGEIRDEFDIDEISEIRKIGEGHYILDGKVLIDQVNDL 375 Query: 391 TGFDLHDDEVTTIGGWLYSHLEEPAIGKSITHENVTLTVREMNRHRIRRVEFELAQ 446 G L ++EV TIGGW + + SI E + E++ H + +E + Q Sbjct: 376 LGIHLENEEVDTIGGWFLTQKYDVEKDDSIIEEGCEFIINEIDGHHVAYIEVKKLQ 431 >BS_yhdT [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 80 Score = 363 bits (932), Expect = e-100, Method: Compositional matrix adjust. Identities = 0/415 (0%), Positives = 0/415 (0%), Gaps = 8/415 (1%) Query: 34 FFVATEFALVRLRGSQISQMVLEGKKNALAVQRVAANLDGYLSACQLGITITALGIGALA 93 + LV S ++ + + L L + + + Sbjct: 19 TLLYLAVWLVAAYLSGVAPGFTGFPRWFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDD 78 Query: 94 EPAFEQLLIPLFDLGNISHSVSEPXXXXXXXXXXXXXHVVVGELAPKTAAINIPEKIGQI 153 Sbjct: 79 138 Query: 154 TSPLIIWFYKILYPLIWLMNGSANLLVRMFGMKPASEHGDAHSEDEIRLILSESYESGKI 213 Sbjct: 139 198 Query: 214 NKAEYGYVNRIFTFDEMLAKEIMVPRTDMVCLFTNHSLKENLEIIRKEQYTRFPVADGSK 273 Sbjct: 199 258 Query: 274 DNIIGMINTKQ-----LYLQYDNNPDFDFKSLILPLLTVSEVTPXXXXTLLTRMQKERVH 328 Sbjct: 259 -- 316 Query: 329 IALLLDEYGGTSGLITIEDILEEIVGEIXXXXXXXXXXNIEKLSDSHYLFDGNVSVLEVK 388 Sbjct: 317 376 Query: 389 ELTGFDLHDDEVTTIGGWLYSHLEEPAIGKSITHENVTLTVREMNRHRIRRVEFE 443 Sbjct: 377 - 430 >lin2334 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 362 bits (930), Expect = e-100, Method: Compositional matrix adjust. Identities = 182/412 (44%), Positives = 269/412 (65%), Gaps = 7/412 (1%) Query: 34 FFVATEFALVRLRGSQISQMVLEGKKNALAVQRVAANLDGYLSACQLGITITALGIGALA 93 FFVATEFA+V++R S++ Q++ E K A+ + + +L+ YLSACQLGITI++LG+G L Sbjct: 19 FFVATEFAIVKMRPSRLDQLIAEKDKRAVLARHIYNHLNAYLSACQLGITISSLGLGWLG 78 Query: 94 EPAFEQLLIPLFDLGNISHSVSEPXXXXXXXXXXXXXHVVVGELAPKTAAINIPEKIG-Q 152 E E L PLF L + S HVVVGEL PKT AI+ E + Sbjct: 79 ESTVEAALHPLFSLLELPQSAITILSFTIAFLFITFLHVVVGELVPKTLAIDKTETVALA 138 Query: 153 ITSPLIIWFYKILYPLIWLMNGSANLLVRMFGMKPASEHGDAHSEDEIRLILSESYESGK 212 + PL I FYK+++P IW++NGSA + R+FG++PASEH AH+EDE+++I+ ESY+SG+ Sbjct: 139 VARPLHI-FYKVMFPFIWILNGSAVFIARLFGLEPASEHEIAHTEDELKIIVGESYKSGE 197 Query: 213 INKAEYGYVNRIFTFDEMLAKEIMVPRTDMVCLFTNHSLKENLEIIRKEQYTRFPVADGS 272 IN++E+ YVN+IF FDE +AKE+M+PRT++V + T ++ E +I++ E+YTR+PV DG Sbjct: 198 INQSEFRYVNKIFDFDERMAKEVMIPRTEIVTVDTGSTIGELSDIMQNERYTRYPVIDGD 257 Query: 273 KDNIIGMINTKQL---YLQYDNNPDFDFKSLILPLLTVSEVTPXXXXTLLTRMQKERVHI 329 KD++IG++N K++ Y+++ +NP F + P++ V E P LL RMQ+ER HI Sbjct: 258 KDHVIGVLNLKEILSAYVEHGSNPSFSIDPYVKPIIRVIETIPIKE--LLFRMQRERSHI 315 Query: 330 ALLLDEYGGTSGLITIEDILEEIVGEIXXXXXXXXXXNIEKLSDSHYLFDGNVSVLEVKE 389 A+LLDEYGGTSGL+T+EDI+EEIVG+I I K+ D HY+ D + + EV Sbjct: 316 AILLDEYGGTSGLVTVEDIVEEIVGDIRDEFDADEIPEIRKIKDGHYIVDAKLLIDEVNN 375 Query: 390 LTGFDLHDDEVTTIGGWLYSHLEEPAIGKSITHENVTLTVREMNRHRIRRVE 441 + G ++ ++EV TIGGW + E +G I ++ V++ H I +E Sbjct: 376 ILGTEIEEEEVDTIGGWFLTQNYEVEVGDEIDYDGFIFRVKQGEPHHIEYIE 427 >BS_yugS [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 353 bits (906), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 182/409 (44%), Positives = 253/409 (61%), Gaps = 2/409 (0%) Query: 35 FVATEFALVRLRGSQISQMVLEGKKNALAVQRVAANLDGYLSACQLGITITALGIGALAE 94 FVA EFA+V++RGS+I+Q++ G ALA ++ +NLD YLSACQLGITITALG+G L E Sbjct: 24 FVAAEFAIVKIRGSKINQLIESGDSRALAAHKIISNLDEYLSACQLGITITALGLGWLGE 83 Query: 95 PAFEQLLIPLFDLGNISHSVSEPXXXXXXXXXXXXXHVVVGELAPKTAAINIPEKIGQIT 154 P FE+ L PLF + I + HVV+GELAPKT +I E + Sbjct: 84 PTFERFLHPLFTMTGIPEPFNHIVTFVVAFIIVTFLHVVMGELAPKTVSIQKAEAVSLWI 143 Query: 155 SPLIIWFYKILYPLIWLMNGSANLLVRMFGMKPASEHGDAHSEDEIRLILSESYESGKIN 214 + +IWFYKI YP I +NGSA+ LV++FG EH SE+E+RLILSESYE G+IN Sbjct: 144 AKPLIWFYKITYPFIKALNGSASFLVKLFGFHSVKEHQVVISEEELRLILSESYEKGEIN 203 Query: 215 KAEYGYVNRIFTFDEMLAKEIMVPRTDMVCLFTNHSLKENLEIIRKEQYTRFPVADGSKD 274 ++E+ YVN+IF FD +A+EIM+PRT++ + SL+E + I E+YTR+PV KD Sbjct: 204 QSEFRYVNKIFEFDNRVAREIMIPRTEIAVISLEQSLEEAIHHIINERYTRYPVIKDDKD 263 Query: 275 NIIGMINTKQLYLQYDNNPDFDFKSLILPLLTVSEVTPXXXXTLLTRMQKERVHIALLLD 334 +I+G+IN+K ++ Y ++ P++ V E P LL RMQKER+H+A+L+D Sbjct: 264 HILGIINSKDMFKAYFLGQPIKLNQIMRPVIRVIESIP--VQQLLIRMQKERIHMAILVD 321 Query: 335 EYGGTSGLITIEDILEEIVGEIXXXXXXXXXXNIEKLSDSHYLFDGNVSVLEVKELTGFD 394 EYGGT+GL+T+EDI+EEIVGEI +I K + HY+ DG + EV +L Sbjct: 322 EYGGTAGLVTVEDIIEEIVGEIRDEYDQDETPHILKKGEHHYVMDGKALIDEVNDLLDIA 381 Query: 395 LHDDEVTTIGGWLYSHLEEPAIGKSITHENVTLTVREMNRHRIRRVEFE 443 + ++E+ TI GWL + E G I E + + H IR VE + Sbjct: 382 IENEEIDTIAGWLLTQKMELKAGDVIHAEGCEFKILDAEDHHIRFVEIK 430 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,800,846 Number of extensions: 1223925 Number of successful extensions: 2056 Number of sequences better than 1.0e-05: 84 Number of HSP's gapped: 1895 Number of HSP's successfully gapped: 84 Length of query: 457 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 347 Effective length of database: 25,691,691 Effective search space: 8915016777 Effective search space used: 8915016777 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)