BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0039.p (410 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE2330 [GEPR] COG0477 Permeases of the major facilitator superf... 251 1e-66 SMa1447 [GEPR] COG0477 Permeases of the major facilitator superf... 211 2e-54 PM0835 [GEPR] COG0477 Permeases of the major facilitator superfa... 120 4e-27 HI1104 [GEPR] COG0477 Permeases of the major facilitator superfa... 114 2e-25 ECs0408 [GEPR] COG0477 Permeases of the major facilitator superf... 106 7e-23 >PAE2330 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 384 Score = 251 bits (641), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 154/397 (38%), Positives = 225/397 (56%), Gaps = 18/397 (4%) Query: 11 KQRKLLFSAGLSWMFDAMDVGMISFVVAALAKEWGLGPERIGYLTSINSIGMAVGXXXXG 70 K+ +L+ GL WMFDA+DV ++S+++ A++ E L + ++ N++GM +G G Sbjct: 2 KEGRLVLYTGLGWMFDALDVLILSYLLVAMSNELKLDSQAKNWIVLANNLGMLIGAMLFG 61 Query: 71 EAAGILADRFGRKSVLLWTXXXXXXXXXXXXXXXXYAMLCVLRFVAGFGLGGELPVASTL 130 LADR GRK V + T + V+RF G GLGGELPV +T Sbjct: 62 R----LADRVGRKKVFMSTLLTYSIATAISAFARNWQEFAVIRFFVGLGLGGELPVVATY 117 Query: 131 VSESMPVKERGRAVVLLESFWAVGWIVSALIAYFVIPDYGWRVAFAIGAVPALYALYLRR 190 VSE+ P + RGR VVLLESFW++G I++A I+ F+ GWR + + A Y +R Sbjct: 118 VSENSPPERRGRNVVLLESFWSLGAILAAAISLFLFTTIGWRSSLLLMGATAFYVFVIRF 177 Query: 191 AIDDSPRFAEIRKKAPVPLRSRIATVWSGEYRRSTIMLWILWFTVVFSYYGMFLWLPTVM 250 + +S R+ E+R++ +RI GE+ R ++ +WF + F YYG FLWLPT++ Sbjct: 178 TLPESQRWLELREQRGGLFETRII----GEFVRRLTLVGAVWFLLAFGYYGAFLWLPTML 233 Query: 251 V-IKGFSLVKSFEYVLIMTLAQLPGYFTAAYFIEKFGRKFVLVIYLLLTXXXXXXXXXXN 309 V +GF+ V ++E++ + TLAQLPGYF+AAY +EK GR+ V +Y L+ N Sbjct: 234 VRERGFTQVGTYEFMFLTTLAQLPGYFSAAYLVEKAGRRPVGSLYFALSALSAAFFISSN 293 Query: 310 STTEGMLMAAGICLSFFNLGAWGGMYAYTPELYPTAIRSTGAGLATSFGRIGGIIAPTLV 369 S L+ + L+FFNLG WG +YAYTPEL+PT++R GLA S RIG I+ P L Sbjct: 294 SYA--ELLLWALALNFFNLGVWGVVYAYTPELFPTSVRGIATGLAGSAARIGMILGPLLY 351 Query: 370 GVMVGNAVGIGTIFMLFFVTIIIGALAVLFLGKETKG 406 + A+ I I L A A++ L ET+G Sbjct: 352 PLYASTALLIIAIAWLV-------ASALILLLPETRG 381 >SMa1447 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 450 Score = 211 bits (537), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 143/430 (33%), Positives = 227/430 (52%), Gaps = 44/430 (10%) Query: 12 QRKLLFSAGLSWMFDAMDVGMISFVVAALAKEWGLGPERIGYLTSINSIGMAVGXXXXGE 71 QR+L+ GL W DAM V + F A++A +GL + ++ +GM G G Sbjct: 32 QRRLMAIFGLVWAADAMQVLAVGFTAASIAATFGLTVPQALQTGTLFFLGMLFGAAGFGR 91 Query: 72 AAGILADRFGRKSVLLWTXXXXXXXXXXXXXXXXYAMLCVLRFVAGFGLGGELPVASTLV 131 LADR GR+ VL+ T + +L +LRF+ G +GG LPV ++ Sbjct: 92 ----LADRIGRRRVLIATVACDAVFGLLSVFAQDFTVLLLLRFLTGAAVGGTLPVDYAMM 147 Query: 132 SESMPVKERGRAVVLLESFWAVGWIVSALIAYFV----IPDYGWRVAFAIGAVPALYALY 187 +E +P + RGR +V+LE FWAVG ++ AL A+ + D WR FA+ A+PAL + Sbjct: 148 AEFLPARNRGRWLVMLEGFWAVGTLIVALAAWAASLAGVAD-AWRYIFAVTAIPALIGVG 206 Query: 188 LRRAIDDSP----RFAEIRKKAPV---------------------PLRSRIATVWSGEYR 222 LR + +SP R + + + P + A ++S + R Sbjct: 207 LRFLVPESPLYLLRLGKTSEAKAIVDEILVVNGKMRLGAGASLVPPPPTASAGIFSADLR 266 Query: 223 RSTIMLWILWFTVVFSYYGMFLWLPTVMVIKGFSLVKSFEYVLIMTLAQLPGYFTAAYFI 282 + ++M+ +WF V SYYG+F W+P + +GF V+ + +++++ LAQ+PGY AAY + Sbjct: 267 KRSLMILAIWFLVSISYYGVFTWMPPRLAGEGFGFVRGYGFLVVLALAQIPGYALAAYGV 326 Query: 283 EKFGRKFVLVIYLLLTXXXXXXXXXXNSTTEGMLMAAGICL---SFFNLGAWGGMYAYTP 339 EK+GR+ L+ + LL+ G M G+ L SF LG WG +YAYTP Sbjct: 327 EKWGRRPTLIGFCLLSALGCLLF-----VAAGTAMLIGVSLLIMSFALLGTWGALYAYTP 381 Query: 340 ELYPTAIRSTGAGLATSFGRIGGIIAPTLVGVMVGNAVGIGTIFMLFFVTIIIGALAVLF 399 ELYPTA R+TG G A + R+GG++AP+L+G++V + G+ +F +++ A+A Sbjct: 382 ELYPTASRATGMGAAGAMARLGGLLAPSLMGLVVAQSFGLA--IGIFAGLLLVAAVAAFL 439 Query: 400 LGKETKGLEL 409 + ET+ + L Sbjct: 440 IDAETRRVSL 449 >PM0835 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 405 Score = 120 bits (301), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 112/407 (27%), Positives = 180/407 (44%), Gaps = 14/407 (3%) Query: 9 NPKQRKLLFSAGLSWMFDAMDVGMISFVVAALAKEWGLGPERIGYLTSINSIGMAVGXXX 68 N K L + + + D D+ ++ F+++A++ + L P + G L + IG G Sbjct: 6 NKDGWKALLGSAVGYAMDGFDLLILGFMLSAISADLALTPAQSGSLVTWTLIGAVAG--- 62 Query: 69 XGEAAGILADRFGRKSVLLWTXXXXXXXXXXXXXXXXYAMLCVLRFVAGFGLGGELPVAS 128 G G L+D++GR VL WT Y L + R +AG GLGGE + Sbjct: 63 -GIIFGALSDKYGRVRVLTWTIVLFAVFTGLCAFAQGYWDLLIYRTIAGIGLGGEFGIGM 121 Query: 129 TLVSESMPVKERGRAVVLLESFWAVGWIVSALIAYFVIPDYGWRVAFAIGAVPALYALYL 188 L +E+ P + R +A + W VG + +AL+ ++P GWR F +G PA A YL Sbjct: 122 ALAAEAWPARHRAKASSYVALGWQVGVLGAALLTPLLLPHIGWRGMFLVGIFPAFVAWYL 181 Query: 189 RRAIDDSPRFAEIRKKAPVPLRSRIA--TVWSGEYRRSTIMLWILWFTVV--FSYYGMFL 244 R + + F + + SR+ + + + I I+ T V F YYG+ + Sbjct: 182 RAKLHEPEIFVKKQTALERQKTSRLEPFRLLIKDKATAKISAGIVVLTSVQNFGYYGIMI 241 Query: 245 WLPTVMVIK-GFSLVKSFEYVLIMTLAQLPGYFTAAYFIEKFGRKFVLVIYLLLTXXXXX 303 W+P + + GFSL KS + + + G + ++ GRK +++ Sbjct: 242 WMPNFLSKQLGFSLTKSGVWTAVTICGMMFGIWLFGQLADRIGRKPSFLLFQFGAVISIF 301 Query: 304 XXXXXNSTTEGMLMAAGICLSFFNLGAWGGMYAYTPELYPTAIRSTGAGLATSFGRIGGI 363 + T ++ AG L F G GG A E YPT R+T + + GR G Sbjct: 302 TYSQMSDPT--YMLFAGAVLGMFVNGMMGGYGALMSEAYPTQARATAQNVLFNLGRAVGG 359 Query: 364 IAPTLVGVMVGNAVGIGTIFMLFFVTI-IIGALAVLFLGKETKGLEL 409 P +VG +V + + F +I II +A +FL E KG +L Sbjct: 360 FGPIVVGALVSTYS--FQLAIAFLASIYIIDMIATIFLIPELKGKQL 404 >HI1104 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 407 Score = 114 bits (286), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 112/408 (27%), Positives = 177/408 (43%), Gaps = 14/408 (3%) Query: 9 NPKQRKLLFSAGLSWMFDAMDVGMISFVVAALAKEWGLGPERIGYLTSINSIGMAVGXXX 68 N K L + + + D D+ ++ F+++A++ + L P + G L + IG G Sbjct: 6 NSYGWKALIGSAVGYGMDGFDLLILGFMLSAISADLNLTPAQGGSLVTWTLIGAVFG--- 62 Query: 69 XGEAAGILADRFGRKSVLLWTXXXXXXXXXXXXXXXXYAMLCVLRFVAGFGLGGELPVAS 128 G G L+D++GR VL WT Y L + R +AG GLGGE + Sbjct: 63 -GILFGALSDKYGRVRVLTWTILLFAVFTGLCAIAQGYWDLLIYRTIAGIGLGGEFGIGM 121 Query: 129 TLVSESMPVKERGRAVVLLESFWAVGWIVSALIAYFVIPDYGWRVAFAIGAVPALYALYL 188 L +E+ P + R +A + W VG + +AL+ ++P GWR F +G PA A +L Sbjct: 122 ALAAEAWPARHRAKAASYVALGWQVGVLGAALLTPLLLPHIGWRGMFLVGIFPAFVAWFL 181 Query: 189 RRAIDDSPRFAE----IRKKAPVPLRSRIATVWSGEYRRSTIMLWILWFTVV--FSYYGM 242 R + + F + + ++ + R + + S I L I+ T V F YYG+ Sbjct: 182 RSHLHEPEIFTQKQTALSTQSSFTDKLRSFQLLIKDKATSKISLGIVVLTSVQNFGYYGI 241 Query: 243 FLWLPTVMVIK-GFSLVKSFEYVLIMTLAQLPGYFTAAYFIEKFGRKFVLVIYLLLTXXX 301 +WLP + + GFSL KS + + + G + ++ GRK +++ L Sbjct: 242 MIWLPNFLSKQLGFSLTKSGLWTAVTVCGMMAGIWIFGQLADRIGRKPSFLLFQL--GAV 299 Query: 302 XXXXXXXNSTTEGMLMAAGICLSFFNLGAWGGMYAYTPELYPTAIRSTGAGLATSFGRIG 361 T +++ AG L F G GG A E YPT R+T + + GR Sbjct: 300 ISIVVYSQLTDPDIMLLAGAFLGMFVNGMLGGYGALMAEAYPTEARATAQNVLFNIGR-A 358 Query: 362 GIIAPTLVGVMVGNAVGIGTIFMLFFVTIIIGALAVLFLGKETKGLEL 409 +V V A T L + +I LA +FL E KG L Sbjct: 359 VGGFGPVVVGSVVLAYSFQTAIALLAIIYVIDMLATIFLIPELKGKAL 406 >ECs0408 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 106 bits (264), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 88/310 (28%), Positives = 142/310 (45%), Gaps = 11/310 (3%) Query: 74 GILADRFGRKSVLLWTXXXXXXXXXXXXXXXXYAMLCVLRFVAGFGLGGELPVASTLVSE 133 G+LADR+GRK +L+ + + L R + G GLG LP L SE Sbjct: 86 GMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAALPNLIALTSE 145 Query: 134 SMPVKERGRAVVLLESFWAVGWIVSALIAYFVIPDYGWRVAFAIGAV-PALYALYLRRAI 192 + + RG AV L+ +G ++A + F + W+ F +G V P + L R + Sbjct: 146 AAGPRFRGTAVSLMYCGVPIGAALAATLG-FAGANLAWQTVFWVGGVVPLILVPLLMRWL 204 Query: 193 DDSPRFAEIRKKAPVPLRSRIATVWSGEYRRSTIMLWILWFTVVFSYYGMFLWLPTVMVI 252 +S FA ++ AP PLR+ A E +T++LW+ +F + Y + WLP ++V Sbjct: 205 PESAVFAGEKQAAP-PLRALFAP----ETATATLLLWLCYFFTLLVVYMLINWLPLLLVE 259 Query: 253 KGFSLVKSFEYVLIMTLAQLPGYFTAAYFIEKFGRKFVLVIYLLLTXXXXXXXXXXNSTT 312 +GF ++ + + + G ++K R + + + S+ Sbjct: 260 QGFQPSQAAGVMFALQMGAASGTLMLGALMDKL-RPVTMSLLIYSGMLASLLALGTVSSF 318 Query: 313 EGMLMAAGICLSFFNLGAWGGMYAYTPELYPTAIRSTGAGLATSFGRIGGIIAPTLVGVM 372 GML+A G F G +YA P Y + IR+TG G A + GR+G + P L G M Sbjct: 319 NGMLLA-GFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAGKM 377 Query: 373 VGNAVGIGTI 382 + A+G GT+ Sbjct: 378 L--ALGTGTV 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,504,885 Number of extensions: 1096293 Number of successful extensions: 16304 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 16259 Number of HSP's successfully gapped: 73 Length of query: 410 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 301 Effective length of database: 25,807,025 Effective search space: 7767914525 Effective search space used: 7767914525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)