BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0036.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH312 [R] COG1201 Lhr-like helicases 197 2e-50 PAE2130 [R] COG1201 Lhr-like helicases 180 4e-45 PH1329 [R] COG1201 Lhr-like helicases 166 8e-41 SSO0965 [R] COG1201 Lhr-like helicases 157 3e-38 PAB1817 [R] COG1201 Lhr-like helicases 154 3e-37 >MTH312 [R] COG1201 Lhr-like helicases Length = 254 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 0/355 (0%), Positives = 0/355 (0%), Gaps = 40/355 (11%) Query: 9 LAPFIKEFIYKNR--WETLREAQVDACRVLFDTPHHLLIASGTASGKTEAAFFPALTQLY 66 + + + E + D + + + A + Sbjct: 6 YSLEGEAID-EMELPEIFNEEFRPDVIKRAVLSAQTARVQP-WGPDPMAGKRTSAQSYGA 63 Query: 67 EDPSASVGILYIAPLKALINDQFTRLNDLLREGNIPVWHWHGDVPQADKTKLMQNPSGVL 126 A V + A + + + + Sbjct: 64 GRGVAMVPRIKNGSRAAFVPQAVGGRRAHPPRPQKNYHERINRKERRLAIRSAVAATARK 123 Query: 127 QITPESLEGLLMNRPNA-IPALFHDLRFIIVDEVHAFMGADRGIQVLSQLARISRMAGCH 185 + + + + D F ++ AG Sbjct: 124 DLVEARGHRIENVPQLPLVVDDELSMIKRTADTREVFRKLGIMDDIVRAREGKKIRAGKG 183 Query: 186 PRRIGLSATLSDYASVTEWLA---AGTRESVEVSAPQGGRKLRLSVEHFSFPDARDEVQA 242 R T V G R + Sbjct: 184 KMRGRKYRTPRGPLIVVGDDKGITRGARNHPG-------VDVVRVEN---------LNAE 227 Query: 243 EHLERARQAYYGFIYDHTHLKKALIFTNSRTDAEEAILEXXXXRLAAKRGERDVFHVHHG 302 + + Sbjct: 228 LLAPGTHPGRLT--------VFTRSAIEKLDELFQ------- 272 Query: 303 SISAMLREETEAALRQGPGPAVAAATLTLELGIDLGELERVLQLGAPYSCASFVQ 357 Sbjct: 273 - 326 >PAE2130 [R] COG1201 Lhr-like helicases Length = 914 Score = 180 bits (456), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 140/381 (36%), Positives = 197/381 (51%), Gaps = 19/381 (4%) Query: 6 FYRLAPFIKEFIYKNRWETLREAQVDACRVLFDTPHHLLIASGTASGKTEAAFFPALTQL 65 F L P +++ I + + AQ+ A + H LLIA T GKTEAAFFP L+++ Sbjct: 3 FNLLHPELQKAIKELGYAEPTPAQIGAIPKILAGLHVLLIAP-TGFGKTEAAFFPVLSRI 61 Query: 66 YED-PSASVGILYIAPLKALINDQFTRLNDLLREGNIPVWHWHGDVPQADKTKLMQNPSG 124 E V LYI PL++L D RL L + + HGD P+ ++ L + P Sbjct: 62 LESRGEGGVKCLYITPLRSLNRDLLRRLFALAERVGVSIAVRHGDTPEGERRSLARKPPD 121 Query: 125 VLQITPESLEGLLMNRPNAIPALFHDLRFIIVDEVHAFMGADRGIQVLSQLARISRMAGC 184 VL TPE+L+ LL++R P+L +RF+IVDEVH + + RG+Q+ L R+ +AG Sbjct: 122 VLITTPETLQILLLHR-GLRPSL-RKVRFVIVDEVHELINSKRGVQLTVGLERLVELAG- 178 Query: 185 HPRRIGLSATLSDYASVTEWLAAGTRESVEVSAPQGGRKLRLSVEHFSFPDARDEVQAEH 244 +RIGLSAT+ V E+L G VEV G +KL + V P D + AE Sbjct: 179 EFQRIGLSATVGAPGLVAEFLG-GVGRRVEVVDVSGEKKLDIEVV-LPTPSDEDYIDAEK 236 Query: 245 LERARQAYYGFIYDHTHLKKA----LIFTNSRTDAEEAILEXXXXRLAAKRGERDVFHVH 300 + +A ++K + L+FTN+R AE RL G DV VH Sbjct: 237 FDATPEAVARIKRVGEYVKSSKGSVLVFTNTRDGAE-----FLASRLKQILG--DVVEVH 289 Query: 301 HGSISAMLREETEAALRQGPGPAVAAATLTLELGIDLGELERVLQLGAPYSCASFVQRLG 360 H S+S R E L++G AV A T +LELGID+GE++ V+Q G+P A VQR+G Sbjct: 290 HSSLSRDHRIAVEERLKRGELKAVVA-TSSLELGIDIGEIDLVVQYGSPRQVAKLVQRVG 348 Query: 361 RSGRRGDAASEMIFVTPEEED 381 RSG + S I + + ED Sbjct: 349 RSGHKLGLTSRGIVIAADLED 369 >PH1329 [R] COG1201 Lhr-like helicases Length = 912 Score = 166 bits (419), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 122/370 (32%), Positives = 182/370 (49%), Gaps = 30/370 (8%) Query: 19 KNRWETLREAQVDACRVLFDTPHHLLIASGTASGKTEAAFFPALTQLYEDPSASVGILYI 78 K ++ L + Q+ A + ++ +LI + T SGKTEAA P + + +D + LYI Sbjct: 10 KEKFGKLNKLQIQAFKAIYGEGESVLIIAPTGSGKTEAAIIPVINSILKDNLRPISCLYI 69 Query: 79 APLKALINDQFTRLNDLLREGNIPVWHWHGDVPQADKTKLMQNPSGVLQITPESLEGLLM 138 APLKAL D RL + I V HGD PQ+ + K ++NP +L TPESL +L Sbjct: 70 APLKALNRDLLERLKWWGEKTGIKVEVRHGDTPQSMRAKQVKNPPHILITTPESLPAILT 129 Query: 139 NRPNAIPALFHDLRFIIVDEVHAFMGADRGIQVLSQLARISRMAGCHPRRIGLSATLSDY 198 + ++ +++F+IVDE+ + RGIQ++ L R+S +A RIGLSAT+ + Sbjct: 130 TK--SLRKYLENVKFVIVDEITELVDNKRGIQLILNLKRLSLIADFI--RIGLSATVGNE 185 Query: 199 ASVTEWLAAGTRESVEVSAPQGGRKLRLSVEHFSFPDARDEVQAEHLE-------RARQA 251 + EW E+ + P ++ + V + P D+ AE L+ R R Sbjct: 186 EEIREWF-----EAKRIIKPHLKKRYKFKV-LYPQPKPEDQELAEKLKVPGDVATRLRTL 239 Query: 252 YYGFIYDHTHLKKALIFTNSRTDAEEAILEXXXXRLAAKRGERDVFHVHHGSISAMLREE 311 + KKALIF N+R AE IL VHHGS+S R E Sbjct: 240 WEIV----EKYKKALIFVNTRQFAE--ILGHRLKTWGKP------VEVHHGSLSKEARIE 287 Query: 312 TEAALRQGPGPAVAAATLTLELGIDLGELERVLQLGAPYSCASFVQRLGRSGRRGDAASE 371 E L++G A+ T ++ELGID+G+++ V+Q +P +QR GRS R SE Sbjct: 288 AERKLKEGKIKAL-ICTSSMELGIDIGDVDVVIQYMSPRQVNRLIQRAGRSRHRLWEVSE 346 Query: 372 MIFVTPEEED 381 +T ED Sbjct: 347 AYIITTGVED 356 >SSO0965 [R] COG1201 Lhr-like helicases Length = 933 Score = 157 bits (398), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 109/351 (31%), Positives = 175/351 (49%), Gaps = 20/351 (5%) Query: 13 IKEFIYKNRWETLREAQVDACRVLFDTPHHLLIASGTASGKTEAAFFPALTQLYEDPSAS 72 +++ I + W L Q + + + + L+IA T GKTEAA P L ++ ++ Sbjct: 9 LRKLIEEKNWMKLTRVQEMSFEPISNGYNTLVIAP-TGYGKTEAALLPILNRMLKEDVKP 67 Query: 73 VGILYIAPLKALINDQFTRLNDLLREGNIPVWHWHGDVPQADKTKLMQNPSGVLQITPES 132 V ++YI PLKALIND R++ V HG+VPQ +K ++ ++ TPE Sbjct: 68 VTVIYITPLKALINDLLYRIDWWASRLGFLVNRKHGEVPQKEKNLRLKRIPHIIVTTPEG 127 Query: 133 LEGLLMNRPNAIPALFHDLRFIIVDEVHAFMGADRGIQVLSQLARISRMAGCHPRRIGLS 192 LE + ++ + + ++++++VDE+H + + RG Q+ L R+ G +RIGLS Sbjct: 128 LE-IDLDWASRFREHYKNVKWVVVDEIHELINSKRGTQLSILLERLKYFIGYDFQRIGLS 186 Query: 193 ATLSDYASVTEWLAAGTRESVEVSAPQGGRKLRLSVEHFSFPDARDEVQAEHLERARQAY 252 AT+SD V ++L + +++ R + + D + E+ Q Sbjct: 187 ATVSDEDKVAQFLFGSSERKMKIIRLNDTRNFEIKINKLG-NDNSFALWKSAAEKINQLI 245 Query: 253 YGFIYDHTHLKKALIFTNSRTDAEEAILEXXXXRLAAKRGERDVFHVHHGSISAMLREET 312 K L+FTNSR E E K G ++++ VHH S+S L+ Sbjct: 246 E---------KPTLVFTNSRFSTERLHEE------LEKLGNKEIY-VHHSSVSRDLKNLA 289 Query: 313 EAALRQGPGPAVAAATLTLELGIDLGELERVLQLGAPYSCASFVQRLGRSG 363 E LR G AV T TLELGI +G++++V+ L P S +SF+QRLGRSG Sbjct: 290 EGELRNGNAKAV-VCTKTLELGIHVGDIKKVIMLRPPTSVSSFLQRLGRSG 339 >PAB1817 [R] COG1201 Lhr-like helicases Length = 912 Score = 154 bits (388), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 125/369 (33%), Positives = 182/369 (49%), Gaps = 29/369 (7%) Query: 19 KNRWETLREAQVDACRVLFDTPHHLLIASGTASGKTEAAFFPALTQLYEDPSASVGILYI 78 K ++ L Q++A + + + L+IA T SGKTEAA P + + +D + LYI Sbjct: 10 KEKFRELNRLQIEAFKRISEGKSVLIIAP-TGSGKTEAAILPVMNSILKDGLPPISCLYI 68 Query: 79 APLKALINDQFTRLNDLLREGNIPVWHWHGDVPQADKTKLMQNPSGVLQITPESLEGLLM 138 APLKAL D RL + I + HGD Q + K ++NP VL TPE+L LL Sbjct: 69 APLKALNRDLLERLKWWEAKTGIRIDVRHGDTTQYRRAKQVRNPPHVLITTPETLPALLT 128 Query: 139 NRPNAIPALFHDLRFIIVDEVHAFMGADRGIQVLSQLARISRMAGCHPRRIGLSATLSDY 198 + ++ ++RF++VDEV + RG Q++ L R+S +A RIGLSAT+ + Sbjct: 129 VK--SLRPYLKNVRFVVVDEVTELIDNKRGAQLILNLKRLSLIADFI--RIGLSATVGNE 184 Query: 199 ASVTEWLAAGTRESVEVSAPQGGRKLRLSVEHFSFPDARDEVQAEHLE-----RAR-QAY 252 V EWL E+ P ++ + V F P D+ AE L AR + Sbjct: 185 EEVKEWLGVD-----EIVKPPLKKRYKFKV-LFPRPRPEDKELAELLRVDVEVAARLRTL 238 Query: 253 YGFIYDHTHLKKALIFTNSRTDAEEAILEXXXXRLAAKRGERDVFHVHHGSISAMLREET 312 + + H K+ L+F N+R A E RL K + V VHHGS+S R E Sbjct: 239 WEIVEKH---KRVLVFVNTRQFA-----EVLAHRL--KTWGKPV-EVHHGSLSKEARIEA 287 Query: 313 EAALRQGPGPAVAAATLTLELGIDLGELERVLQLGAPYSCASFVQRLGRSGRRGDAASEM 372 E L++G A+ T ++ELGID+GE++ V+Q +P +QR GRS R SE Sbjct: 288 ERKLKEGKVKAL-ICTSSMELGIDIGEVDAVIQYMSPRQVNRLIQRAGRSKHRLLEVSEA 346 Query: 373 IFVTPEEED 381 + ED Sbjct: 347 YIIATGVED 355 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,298,987 Number of extensions: 1146427 Number of successful extensions: 3364 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 3299 Number of HSP's successfully gapped: 70 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)