BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0017.p (432 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2228 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosida... 679 0.0 BS_melA [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosid... 649 0.0 mlr6450 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosid... 453 e-127 SMb21643 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosi... 440 e-123 AGl436 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosida... 433 e-121 >BH2228 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 434 Score = 679 bits (1753), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/434 (73%), Positives = 366/434 (84%), Gaps = 2/434 (0%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M KITF+GAGST+FAKNVLGDCM T +LQ FE ALFDIDL+RL DS ML ++K + + Sbjct: 1 MGKITFLGAGSTIFAKNVLGDCMLTPSLQEFEFALFDIDLDRLHDSETMLKHLKETLQAN 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 ++AYTDRKEALRGAKYV+NAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFR+ Sbjct: 61 VKIEAYTDRKEALRGAKYVVNAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRS 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPVMLDFA D+ EVCPDA LNYTNPMA LT M Y GV+TVGLCHSVQ C P L Sbjct: 121 LRTIPVMLDFATDMEEVCPDAWLLNYTNPMATLTGTMLRYTGVKTVGLCHSVQVCAPELL 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQK-EHHHDMVRYEMML 239 + LGM++ +Q KIAGINHMAWLLE+T++GEDLYPEIKRRA EKQ+ E H DMVR+E+M Sbjct: 181 DRLGMEKDNIQWKIAGINHMAWLLEITREGEDLYPEIKRRAKEKQQNERHDDMVRFELMF 240 Query: 240 KFGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHD 299 +FGYY+TESSEHNAEY PYFIK YPELIER IPLDEYPRRC+ QI WK+MR++LV++ Sbjct: 241 RFGYYVTESSEHNAEYFPYFIKEKYPELIERLNIPLDEYPRRCINQIEDWKKMRKDLVNN 300 Query: 300 TTLHHERSHEYASYILEAIETNVPFKIGGNVLNTG-LITNLPREACVEVPCLVDASGVTP 358 + HER+HEYASYI+EA+ETN PF+IGGNVLN G LI NLP ACVEVPCLVD +G+ P Sbjct: 301 KHITHERTHEYASYIMEAMETNKPFQIGGNVLNKGRLIANLPENACVEVPCLVDRNGIQP 360 Query: 359 TYVGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCD 418 TYVG+LP Q AALNRTNINTQLLTIEAA KKE IY AAMLDPHTS+ELS+DDIV++CD Sbjct: 361 TYVGELPEQLAALNRTNINTQLLTIEAAMKRKKEAIYQAAMLDPHTSSELSIDDIVSLCD 420 Query: 419 ELIAAHGQWLPDFK 432 +LI AHG WL FK Sbjct: 421 DLIEAHGSWLQSFK 434 >BS_melA [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 432 Score = 649 bits (1674), Expect = 0.0, Method: Compositional matrix adjust. Identities = 300/432 (69%), Positives = 352/432 (81%) Query: 1 MNKITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGST 60 M KITFIGAGST+FAKNVLGDC+ T AL GFE AL+DID +RL++S+ ML N++ + Sbjct: 1 MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPS 60 Query: 61 CIVKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRN 120 + +Y DRK AL+ A YVINAIQVGGY P T+ DFEIPK+YGLRQTIADTVGIGGIFR+ Sbjct: 61 VAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRS 120 Query: 121 LRTIPVMLDFAADIREVCPDALFLNYTNPMAVLTNVMNTYGGVQTVGLCHSVQHCIPGLF 180 LRTIPV+ D A D+ E+CPDA FLNYTNPMA LT M Y ++T+GLCHSVQ C LF Sbjct: 121 LRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRYTNIKTIGLCHSVQVCTKDLF 180 Query: 181 EALGMDQTGVQAKIAGINHMAWLLEVTKDGEDLYPEIKRRAAEKQKEHHHDMVRYEMMLK 240 +ALGM+ G++ +IAGINHMAWLLEV KDG DLYPEIKRRA EKQK HHDMVR+E+M K Sbjct: 181 KALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDK 240 Query: 241 FGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWKQMREELVHDT 300 FGYY+TESSEHNAEYHPYFIKRNYPELI QIPLDEYPRRCV+QI W++MR+++V++ Sbjct: 241 FGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNK 300 Query: 301 TLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCLVDASGVTPTY 360 L HERS EY S I+EA+ETN PF GGNVLNTGLITNLP +A VEV C+ D +TP + Sbjct: 301 NLTHERSKEYGSRIIEAMETNEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCF 360 Query: 361 VGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSMDDIVAMCDEL 420 G+LP Q AALNRTNINTQL+TIEAA T KKE +Y AAMLDPHTSAELSM DI++MCD+L Sbjct: 361 AGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAMLDPHTSAELSMKDIISMCDDL 420 Query: 421 IAAHGQWLPDFK 432 AAHG WLP++K Sbjct: 421 FAAHGDWLPEYK 432 >mlr6450 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 461 Score = 453 bits (1166), Expect = e-127, Method: Compositional matrix adjust. Identities = 225/441 (51%), Positives = 297/441 (67%), Gaps = 12/441 (2%) Query: 3 KITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGSTCI 62 +ITFIGAGSTVF KN++GD + AL G AL DI+ +RLE+S ++N + A+ G Sbjct: 11 RITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNKLIATLGVKAK 70 Query: 63 VKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRNLR 122 + Y+D+++AL GA +V+ A Q+GGY+PCTITDFE+PKKYGLRQTIADT+G+GGI R LR Sbjct: 71 AETYSDQRKALAGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTLGVGGIMRGLR 130 Query: 123 TIPVMLDFAADIREVCPDALFLNYTNPMAVLT-NVMNTYGGVQTVGLCHSVQHCIPGLFE 181 T+P + D+ VCP+A+ L Y NPMA+ T + Y ++ VGLCHSVQ L Sbjct: 131 TVPHLWKICEDMLAVCPEAIMLQYVNPMAINTWAISEKYPTIKQVGLCHSVQGTAMELAH 190 Query: 182 ALGMDQTGVQAKIAGINHMAWLLEV---TKDG--EDLYPEIKRRAAEKQKEHHH------ 230 L + ++ + AGINHMA+ L+ DG DLYP++ R E + Sbjct: 191 DLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRAPKPGWNPRCP 250 Query: 231 DMVRYEMMLKFGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWK 290 + VRYEM+ + GY++TESSEH AEY PYFIK P+LIE++ IPLDEYP+RC+EQI +WK Sbjct: 251 NKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPKRCIEQIERWK 310 Query: 291 QMREELVHDTTLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCL 350 + + E S EYAS I+ ++ T P I GNV N G IT+LPR+ EVPCL Sbjct: 311 DQAQAYRSAQRIEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLPRDCAAEVPCL 370 Query: 351 VDASGVTPTYVGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSM 410 VDASG+ PTY+GDLPPQ AL RTNIN Q LT+ A + +EHIYHAAM+DPHT+AEL + Sbjct: 371 VDASGIQPTYIGDLPPQLTALIRTNINVQELTVRALMSENREHIYHAAMMDPHTAAELDL 430 Query: 411 DDIVAMCDELIAAHGQWLPDF 431 D I ++ D+L+AAHG WLP + Sbjct: 431 DQICSLVDDLLAAHGDWLPAW 451 >SMb21643 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 457 Score = 440 bits (1131), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/442 (49%), Positives = 294/442 (66%), Gaps = 13/442 (2%) Query: 3 KITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGSTCI 62 +ITFIGAGSTVF KN++GD + AL AL D++ ERL +S + + + G+ Sbjct: 6 RITFIGAGSTVFMKNIIGDILQRPALSAATIALMDVNPERLAESEIVAGKLARTLGADAR 65 Query: 63 VKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRNLR 122 ++ ++++++AL GA +V+ A Q+GGY+PCT+TDFE+PKKYGLRQTIADT+G+GGI R LR Sbjct: 66 IETHSNQRKALEGADFVVVAFQIGGYEPCTVTDFEVPKKYGLRQTIADTLGVGGIMRGLR 125 Query: 123 TIPVMLDFAADIREVCPDALFLNYTNPMAVLT-NVMNTYGGVQTVGLCHSVQHCIPGLFE 181 T+P + D+ EVCP+A+ L Y NPMA+ T + Y ++ VGLCHSVQ L Sbjct: 126 TVPHLWKICEDMLEVCPEAILLQYVNPMAINTWAIAEKYPAIKQVGLCHSVQGTAFELAR 185 Query: 182 ALGMDQTGVQAKIAGINHMAWLLEV---TKDG--EDLYPEIKR--RAAEKQKEHHHD--- 231 L + ++ + AGINHMA+ L+ KDG DLYP++ R R K H + Sbjct: 186 DLDIPLDEIRYRAAGINHMAFYLKFEHRQKDGSYRDLYPDLIRGYREGRFPKPSHWNPRC 245 Query: 232 --MVRYEMMLKFGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKW 289 VRYEM+ + GY++TESSEH AEY PYFIK P+LIE+F IPLDEYP+RCVEQI +W Sbjct: 246 PNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKDGRPDLIEKFGIPLDEYPKRCVEQIERW 305 Query: 290 KQMREELVHDTTLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPC 349 K T+ S EYAS I+ ++ T P I GN+ N G IT+LP EVPC Sbjct: 306 KGQAAAFKEAKTIEVAESREYASSIMNSVWTGEPSVIYGNLRNNGCITSLPENCAAEVPC 365 Query: 350 LVDASGVTPTYVGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELS 409 LVD+SG+ PTY+G LPPQ AL RTNIN Q LT++A T +EH+YHAAM+DPHT+AEL Sbjct: 366 LVDSSGIQPTYIGALPPQLTALIRTNINVQELTVQALVTENREHLYHAAMMDPHTAAELD 425 Query: 410 MDDIVAMCDELIAAHGQWLPDF 431 +D I ++ D+L+AAH W+P++ Sbjct: 426 LDQIWSLVDDLLAAHRDWIPEW 447 >AGl436 [G] COG1486 Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases Length = 200 Score = 433 bits (1113), Expect = e-121, Method: Compositional matrix adjust. Identities = 0/441 (0%), Positives = 0/441 (0%), Gaps = 12/441 (2%) Query: 3 KITFIGAGSTVFAKNVLGDCMGTAALQGFEPALFDIDLERLEDSRRMLNNIKASTGSTCI 62 +T F + A +G+ + + +A + Sbjct: 23 YRLPEEFFTTRFLVRRIVPTDAEAIFEGWNTDPEVTKYLTWKPHSELGQTQRAIEENYSA 82 Query: 63 VKAYTDRKEALRGAKYVINAIQVGGYDPCTITDFEIPKKYGLRQTIADTVGIGGIFRNLR 122 A T + + I P + + Sbjct: 83 WNAGTSFPAVICHRERPHELIGRIDARPMGHKVSYGWLVRRTWWGRGVASEVVQLAVEHA 142 Query: 123 TIPVMLDFAADIREVCPDALFLNYTNPMAVLT-NVMNTYGGVQTVGLCHSVQHCIPGLFE 181 + +V A Sbjct: 143 LSHPRIFRTEASCDVLNTASARVMEKVGMTKEA 202 Query: 182 ALGMDQTGVQAKIAGINHMAWLLEV---TKDG--EDLYPEIKR--RAAEKQKEHHH---- 230 Sbjct: 203 262 Query: 231 DMVRYEMMLKFGYYITESSEHNAEYHPYFIKRNYPELIERFQIPLDEYPRRCVEQISKWK 290 Sbjct: 263 322 Query: 291 QMREELVHDTTLHHERSHEYASYILEAIETNVPFKIGGNVLNTGLITNLPREACVEVPCL 350 Sbjct: 323 382 Query: 351 VDASGVTPTYVGDLPPQCAALNRTNINTQLLTIEAATTGKKEHIYHAAMLDPHTSAELSM 410 Sbjct: 383 442 Query: 411 DDIVAMCDELIAAHGQWLPDF 431 Sbjct: 443 463 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,230,562 Number of extensions: 1305239 Number of successful extensions: 2355 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 2258 Number of HSP's successfully gapped: 46 Length of query: 432 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 323 Effective length of database: 25,807,025 Effective search space: 8335669075 Effective search space used: 8335669075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)