BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0012.p (534 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_nprB [E] COG3227 Zinc metalloprotease (elastase) 387 e-107 CAC2517 [E] COG3227 Zinc metalloprotease (elastase) 300 3e-81 CAC2290_1 [E] COG3227 Zinc metalloprotease (elastase) 295 2e-79 BS_nprE [E] COG3227 Zinc metalloprotease (elastase) 279 9e-75 MA2075_1 [E] COG3227 Zinc metalloprotease (elastase) 211 2e-54 >BS_nprB [E] COG3227 Zinc metalloprotease (elastase) Length = 346 Score = 387 bits (994), Expect = e-107, Method: Compositional matrix adjust. Identities = 0/540 (0%), Positives = 0/540 (0%), Gaps = 26/540 (4%) Query: 8 LLSGIVALGS--FASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSE-EQVYK 64 L + + I + E Sbjct: 10 SLEVSTLGLGTMTFGEQNSEADAHAQL-----DYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 65 YLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTLHIDKS 124 V L + + S+ + R Q ++ + A Sbjct: 65 TETYVGNWLAK--HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 125 GNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAPEADLY 184 + L + AV+ + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGK-----LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 185 YFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLTGTGTGVNGDTKT 244 + Y + + + + + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 245 FETRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXX-----------XNVWSDRAG 293 F T + + + + Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 Query: 294 VDAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTF 353 Sbjct: 298 357 Query: 354 TLLSGDLDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYTP 413 Sbjct: 358 417 Query: 414 GIPGDALRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTV 473 Sbjct: 418 477 Query: 474 AGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 Sbjct: 478 537 >CAC2517 [E] COG3227 Zinc metalloprotease (elastase) Length = 346 Score = 300 bits (769), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 0/503 (0%), Positives = 0/503 (0%), Gaps = 53/503 (10%) Query: 58 SEEQVYKYLESVKGN-LHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGAD 116 ++ + L + D +Q + + + Y Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDK----GIRPDQALD-RKNIREALHDSLKRLQTDYLDL 128 Query: 117 QTLHIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKT--ISDTEAISAAVTDATQQYGPLGE 174 +H + P V L + E A Sbjct: 129 YQVHWPQRPTNCFGKLGYSWT----DSAPAVSLLDTLDALAEYQRAGKIRYI--GVSNET 182 Query: 175 AQIAPEADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILE---DL 231 A Y + T N ++ + Sbjct: 183 A---FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 232 TGTGTGVNGDTKTFETRLSGSTYQLYDNTRGK--GIVTYTAKNRXXXXXXXXXXXXNVWS 289 T TG +NG S + Y + + R Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 290 D---RAGVDAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFG 346 Sbjct: 300 PFVA 359 Query: 347 DGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIE------ 400 Sbjct: 360 419 Query: 401 ----------GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGSSDNGGVHT 450 Sbjct: 420 479 Query: 451 NSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQSA 510 Sbjct: 480 ---------- 529 Query: 511 TELFGAGSAQVTAVTQAYNAVGV 533 Sbjct: 530 -- 550 >CAC2290_1 [E] COG3227 Zinc metalloprotease (elastase) Length = 346 Score = 295 bits (754), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 0/553 (0%), Positives = 0/553 (0%), Gaps = 67/553 (12%) Query: 24 AAESTERSQPAELFTGSTPI-SITGQSLKTPAGDG--------------SEEQVYKYLES 68 +E+ +Q + + P + + + S Sbjct: 27 NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIAS 86 Query: 69 VKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTLHIDKSGNVT 128 ++ IR + + + + + + + Sbjct: 87 KVSGPSRNNDKGIRPDQALDRKNI----REALHDSLKRLQTDYLDLYQVHWPQRPTNCFG 142 Query: 129 SLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAPEADLYYFIV 188 L + + P V L D + +Y + A Y + Sbjct: 143 KLGYSWTDS------APAVSLLDTLDAL--AEYQRAGKIRY-IGVSNETAFGVMRYLHLA 193 Query: 189 GGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILE---DLTGTGTGVNGDTKTF 245 T N ++ + T TG +NG Sbjct: 194 DKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAG 253 Query: 246 ETRLSGSTYQLYD--NTRGKGIVTYTAKNRXXXXXXXXXXXXNV--WSDRAGVDAHTYAE 301 S + Y T+ R + + T + Sbjct: 254 ARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMD 313 Query: 302 RTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLD 361 Sbjct: 314 373 Query: 362 VVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEG----------------ENWL 405 Sbjct: 374 433 Query: 406 VGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGSSDN-----GGVHTNSGINNKAFY 460 Sbjct: 434 - 492 Query: 461 LVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQ 520 Sbjct: 493 ---------- 542 Query: 521 VTAVTQAYNAVGV 533 Sbjct: 543 555 >BS_nprE [E] COG3227 Zinc metalloprotease (elastase) Length = 346 Score = 279 bits (713), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 0/459 (0%), Positives = 0/459 (0%), Gaps = 34/459 (7%) Query: 90 QQTNSKTGSQHYRLSQYINGIPVYGADQTLHIDKSGNVTSLLGTVVEDVYQTIPQPLVQL 149 SK + I + + Sbjct: 82 LIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD----YLDLYQVHWPQRP 137 Query: 150 KTISDTEAISAAVTDATQQYGPL--GEAQIAPEADLYYF-IVGGEPKLAYKTEVNVLEPE 206 S + A+ + Y + + + + Sbjct: 138 TNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHD 197 Query: 207 PLRIRYFISAEDGTVLFKYNILEDL--TGTGTGVNGDTKTFETRLSGSTYQLYDNTRGKG 264 RI + L ++ + F T Sbjct: 198 LPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNT 257 Query: 265 --IVTYTAKNRXX----XXXXXXXXXXNVWSDRAGVDAHTYAERTYDYYLQHFGRNSLDN 318 + + + L + L Sbjct: 258 LFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKT 317 Query: 319 KGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANL 378 Sbjct: 318 377 Query: 379 EYYGEPGALNESFSDIIGNSIEGENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDR 438 Sbjct: 378 --- 434 Query: 439 Y----TGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLT 494 Sbjct: 435 ---------- 484 Query: 495 TSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 Sbjct: 485 -- 521 >MA2075_1 [E] COG3227 Zinc metalloprotease (elastase) Length = 346 Score = 211 bits (537), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 0/532 (0%), Positives = 0/532 (0%), Gaps = 69/532 (12%) Query: 34 AELFTGS---TPISITGQSLKTPAGDGSEEQVYKYLESVKGNLHLSSKDNIRGKFQIKEQ 90 + V E+ + + K Sbjct: 25 EQNSEADAHAQLDYAVA-QGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLI 83 Query: 91 QTNSKTGSQHYRLSQYINGIPVYGADQTLHIDKSGNVTSLLGTVVEDVYQTIPQPLVQLK 150 + + P D+ + + L T D+ V Sbjct: 84 IA---SKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL------YQVHWP 134 Query: 151 TISDTEAISAAVTDATQQYGPLGEAQIAPEADLYYFIVGGEPKLAYKTEVNVLEPEPLRI 210 + A + K+ Y N +R Sbjct: 135 QRPTNCFGKLGYSWTDSA-----PAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRY 189 Query: 211 RYFISAEDGTVLF------KYNILEDLTGTGTGVNGDTKTFETRLSGSTYQLYDNTRGKG 264 + D + G G Sbjct: 190 LHLADKHDLPRIVTIQNPYSLLNRSFEVGL--------------------AEVSQYEGVE 229 Query: 265 IV---TYTAKNRXXXXXXXXXXXXNVWSDRAGVDAHTYAERTYDYYLQHFGRNSLDNKGL 321 ++ + E+T + Sbjct: 230 LLAYSCLGFGTLTGKYLNGAKPA-GARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDP 288 Query: 322 QIRSSVHYYTSYNNAFWNG--SQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLE 379 + A Sbjct: 289 AQMALAFVRRQPFVASTLLGA 348 Query: 380 YYGEPGALNESFSDIIGNSIEGE----NWLVGDKVYTPGIPGDALRSLANPTLYGQPDKY 435 Sbjct: 349 - 407 Query: 436 SDRYT----------GSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIY 485 Sbjct: 408 467 Query: 486 YNALVYYLTTSS----DFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 Sbjct: 468 519 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,059,264 Number of extensions: 1779988 Number of successful extensions: 3509 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3486 Number of HSP's successfully gapped: 7 Length of query: 534 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 423 Effective length of database: 25,576,357 Effective search space: 10818799011 Effective search space used: 10818799011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 118 (50.1 bits)