BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0009.p (295 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ybiV [R] COG0561 Predicted hydrolases of the HAD superfamily 242 4e-64 Z1044 [R] COG0561 Predicted hydrolases of the HAD superfamily 241 7e-64 ECs0899 [R] COG0561 Predicted hydrolases of the HAD superfamily 241 7e-64 STM0842 [R] COG0561 Predicted hydrolases of the HAD superfamily 235 5e-62 STM0867 [R] COG0561 Predicted hydrolases of the HAD superfamily 216 4e-56 >ybiV [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 271 Score = 242 bits (618), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 120/274 (43%), Positives = 181/274 (66%), Gaps = 11/274 (4%) Query: 1 MNIKLIAVDMDGTFLDDKKNYHQKWFEQLYAVMKAKGVHFVVASGNQYYKLKTIFEDIQD 60 M++K+I DMDGTFL+D K Y+Q F Y +K +G+ FVVASGNQYY+L + F +++D Sbjct: 1 MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVASGNQYYQLISFFPELKD 60 Query: 61 ELAYVAENGAYVIDGTEVLFTGEITPEQVKLIVEKLSQYEEISAVMCGVNGAYVLESASK 120 E+++VAENGA V + + LF GE+T + ++++ +L + ++++ V CG+ AYV E+A + Sbjct: 61 EISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQLNFVACGLQSAYVSENAPE 120 Query: 121 EFYELMCHYYPRLEKVSAFEQVNDQIFKFSLITPDLAAGGVDKYLPLLMEE----LKGVV 176 F LM +Y RL+ V +++++D +FKFSL P D+ +PL++++ L G++ Sbjct: 121 AFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLP-------DEQIPLVIDKLHVALDGIM 173 Query: 177 DPVTSGHQCIDLIIPGCHKASGLERLLKRYGVRPDECAAFGDSSNDIEMLKLCKYSYAME 236 PVTSG IDLIIPG HKA+G+ RLLKR+ + P A GDS ND EMLK+ +YS+AM Sbjct: 174 KPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGNDAEMLKMARYSFAMG 233 Query: 237 NASPEVKAVVNYHTVSNNQHGVLHEISLLLGEPS 270 NA+ +K + Y T NN G L+ I +L S Sbjct: 234 NAAENIKQIARYATDDNNHEGALNVIQAVLDNTS 267 >Z1044 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 271 Score = 241 bits (616), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 120/274 (43%), Positives = 181/274 (66%), Gaps = 11/274 (4%) Query: 1 MNIKLIAVDMDGTFLDDKKNYHQKWFEQLYAVMKAKGVHFVVASGNQYYKLKTIFEDIQD 60 M++K+I DMDGTFL+D K Y+Q F Y +K +G+ FVVASGNQYY+L + F +++D Sbjct: 1 MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVASGNQYYQLISFFPELKD 60 Query: 61 ELAYVAENGAYVIDGTEVLFTGEITPEQVKLIVEKLSQYEEISAVMCGVNGAYVLESASK 120 E+++VAENGA V + + LF GE+T + ++++ +L + ++++ V CG+ AYV E+A + Sbjct: 61 EISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQLNFVACGLQSAYVSENAPE 120 Query: 121 EFYELMCHYYPRLEKVSAFEQVNDQIFKFSLITPDLAAGGVDKYLPLLMEE----LKGVV 176 F LM +Y RL+ V +++++D +FKFSL P D+ +PL++++ L G++ Sbjct: 121 AFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLP-------DEQIPLVIDKLHIALDGIM 173 Query: 177 DPVTSGHQCIDLIIPGCHKASGLERLLKRYGVRPDECAAFGDSSNDIEMLKLCKYSYAME 236 PVTSG IDLIIPG HKA+G+ RLLKR+ + P A GDS ND EMLK+ +YS+AM Sbjct: 174 KPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGNDAEMLKMARYSFAMG 233 Query: 237 NASPEVKAVVNYHTVSNNQHGVLHEISLLLGEPS 270 NA+ +K + Y T NN G L+ I +L S Sbjct: 234 NAAENIKQIARYATDDNNHEGALNVIQAVLDNTS 267 >ECs0899 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 271 Score = 241 bits (616), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 120/274 (43%), Positives = 181/274 (66%), Gaps = 11/274 (4%) Query: 1 MNIKLIAVDMDGTFLDDKKNYHQKWFEQLYAVMKAKGVHFVVASGNQYYKLKTIFEDIQD 60 M++K+I DMDGTFL+D K Y+Q F Y +K +G+ FVVASGNQYY+L + F +++D Sbjct: 1 MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVASGNQYYQLISFFPELKD 60 Query: 61 ELAYVAENGAYVIDGTEVLFTGEITPEQVKLIVEKLSQYEEISAVMCGVNGAYVLESASK 120 E+++VAENGA V + + LF GE+T + ++++ +L + ++++ V CG+ AYV E+A + Sbjct: 61 EISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQLNFVACGLQSAYVSENAPE 120 Query: 121 EFYELMCHYYPRLEKVSAFEQVNDQIFKFSLITPDLAAGGVDKYLPLLMEE----LKGVV 176 F LM +Y RL+ V +++++D +FKFSL P D+ +PL++++ L G++ Sbjct: 121 AFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLP-------DEQIPLVIDKLHIALDGIM 173 Query: 177 DPVTSGHQCIDLIIPGCHKASGLERLLKRYGVRPDECAAFGDSSNDIEMLKLCKYSYAME 236 PVTSG IDLIIPG HKA+G+ RLLKR+ + P A GDS ND EMLK+ +YS+AM Sbjct: 174 KPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGNDAEMLKMARYSFAMG 233 Query: 237 NASPEVKAVVNYHTVSNNQHGVLHEISLLLGEPS 270 NA+ +K + Y T NN G L+ I +L S Sbjct: 234 NAAENIKQIARYATDDNNHEGALNVIQAVLDNTS 267 >STM0842 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 269 Score = 235 bits (600), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 117/270 (43%), Positives = 175/270 (64%), Gaps = 11/270 (4%) Query: 1 MNIKLIAVDMDGTFLDDKKNYHQKWFEQLYAVMKAKGVHFVVASGNQYYKLKTIFEDIQD 60 M + ++ DMDGTFLDD K Y + F Y +K + + FVVASGNQYY+L + F +++D Sbjct: 1 MTVNVVVTDMDGTFLDDAKQYDRVRFMAQYQELKKRNIEFVVASGNQYYQLISFFPELKD 60 Query: 61 ELAYVAENGAYVIDGTEVLFTGEITPEQVKLIVEKLSQYEEISAVMCGVNGAYVLESASK 120 E+++VAENGA V + + LF GE+T + ++++ +L + ++++ V CG+ AYV ++A + Sbjct: 61 EISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQLNFVACGLKSAYVSKNAPE 120 Query: 121 EFYELMCHYYPRLEKVSAFEQVNDQIFKFSLITPDLAAGGVDKYLPLLMEE----LKGVV 176 F LM +Y RL+ V+ + ++D +FKFSL P D+ +PL++++ L G++ Sbjct: 121 TFVALMAKHYHRLQPVNDYHDIDDILFKFSLNLP-------DEQIPLVIDKLHVSLDGIM 173 Query: 177 DPVTSGHQCIDLIIPGCHKASGLERLLKRYGVRPDECAAFGDSSNDIEMLKLCKYSYAME 236 PVTSG IDLIIPG HKA+G+ RLLKR+ P A GDS ND EMLK+ YS+AM Sbjct: 174 KPVTSGFGFIDLIIPGLHKANGISRLLKRWNRSPQNVVAIGDSGNDAEMLKMAHYSFAMG 233 Query: 237 NASPEVKAVVNYHTVSNNQHGVLHEISLLL 266 NA+ +KA+ YHT NN G L+ I +L Sbjct: 234 NAADNIKALSRYHTDDNNHQGALNVIQAVL 263 >STM0867 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 271 Score = 216 bits (549), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 120/270 (44%), Positives = 160/270 (59%), Gaps = 4/270 (1%) Query: 1 MNIKLIAVDMDGTFLDDKKNYHQKWFEQLYAVMKAKGVHFVVASGNQYYKLKTIFEDIQD 60 M+IKLIAVDMDGTFL D+K Y++ F Y MK +G+ FVVASGNQYY+L + F +I Sbjct: 1 MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAH 60 Query: 61 ELAYVAENGAYVIDGTEVLFTGEITPEQVKLIVEKLSQYEEISAVMCGVNGAYVLESASK 120 E+A+VAENG +V+ E +F GE+T + +VE L ++ + CG N AY L+ Sbjct: 61 EIAFVAENGGWVVSEGEDIFNGELTKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDD 120 Query: 121 EFYELMCHYYPRLEKVSAFEQVNDQIFKFSLITPDLAAGGVDKYLPLLMEELKGVVDPVT 180 + YY RLE V F +ND FKF L D + + L + + ++ PV Sbjct: 121 ALKAVAAMYYHRLEFVDNFNNINDVFFKFGLNITDER---IPEVQAALHDAIGDIMVPVH 177 Query: 181 SGHQCIDLIIPGCHKASGLERLLKRYGVRPDECAAFGDSSNDIEMLKLCKYSYAMENASP 240 +G+ IDLIIPG HKA+GL L +R+G+ E FGD NDIEML+ +S+AM +AS Sbjct: 178 TGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASE 237 Query: 241 EVKAVVNYHTVSNNQHGVLHEI-SLLLGEP 269 V A Y SNNQ GVL I S+L EP Sbjct: 238 AVAAAAKYRAGSNNQEGVLDIIDSVLNNEP 267 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,092,716 Number of extensions: 880221 Number of successful extensions: 4354 Number of sequences better than 1.0e-05: 119 Number of HSP's gapped: 4276 Number of HSP's successfully gapped: 120 Length of query: 295 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 189 Effective length of database: 26,153,027 Effective search space: 4942922103 Effective search space used: 4942922103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)