BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0003.p (352 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr6806 [E] COG0075 Serine-pyruvate aminotransferase/archaeal as... 139 6e-33 NMA1816_2 [QR] COG0500 SAM-dependent methyltransferases 125 1e-28 CAC0221 [E] COG0075 Serine-pyruvate aminotransferase/archaeal as... 106 5e-23 PAE3447 [E] COG0075 Serine-pyruvate aminotransferase/archaeal as... 104 2e-22 DR1350 [E] COG0075 Serine-pyruvate aminotransferase/archaeal asp... 86 6e-17 >mlr6806 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 301 Score = 139 bits (350), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 0/319 (0%), Positives = 0/319 (0%), Gaps = 12/319 (3%) Query: 25 KLLYTQQPPVIIPGEAILGIEAVAAGIAAPGRTFVNVVTGPYGRLFGQWLERGGATVVEV 84 AA + + Sbjct: 54 GAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLR 113 Query: 85 KVPFDEVAKPDVIASAIEQY-QPDALSFVHAEVVTGGSNPAQDIFKLAQKYKLITVSDSV 143 + + + + + E+ L F+ +++ P L V + Sbjct: 114 EGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHL 173 Query: 144 SAVGGEALNVDEWGVDFAVIGAQKALAGPNGVSSVSISPRGWEFLESNPAAPRNSILSLL 203 + ++ + +E + R Sbjct: 174 PEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDING 233 Query: 204 DLKPSPDGAAPLRVPPNIPTLEARALIAALTEVEEEGLQQVIKRHERXXXXXXXXXXXLN 263 + +G + + + + + + +E + + Sbjct: 234 LILVEREGQKKMVIGNKGA--KIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERA 291 Query: 264 LEPWQKDSRHYSTLTTTIRI-SGKEGLLIRQPT----GIV-APGDGELFGQLLRINHFGA 317 Sbjct: 292 351 Query: 318 NASRDSVEKAIAALARLLG 336 Sbjct: 352 --- 367 >NMA1816_2 [QR] COG0500 SAM-dependent methyltransferases Length = 391 Score = 125 bits (313), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 93/316 (29%), Positives = 154/316 (48%), Gaps = 16/316 (5%) Query: 16 FNTLTGLLSKLLYTQQPPVIIPGEAILGIEAVAAGIAAPGRTFVNVVTGPYGRLFGQWLE 75 + +L+ + T+ ++ G G+EA +A G + + G +G+ F + L Sbjct: 48 YTDCRQILADVFQTKNDIFLLSGSGTAGMEAAVGSVAGSGDKVIAIENGKFGQRF-KDLA 106 Query: 76 RGGATVVEVKVPFDEVAKPDVIASAIEQYQPDALSFVHAEVVTGGSNPAQDIFKLAQKYK 135 A VV ++ + +++ +E+ A++ VH E G NPA +I KLA+K+ Sbjct: 107 ALYADVVPLEFEWGLPVDLEMVKEKLEE-GAKAITLVHNETSAGIMNPAVEIGKLAKKHD 165 Query: 136 LITVSDSVSAVGGEALNVDEWGVDFAVIGAQKALAGPNGVSSVSISPRGWEFLESNPAAP 195 I + D V+++GG+ + VDEWGVD A++G+QK LA P G+S+VS+S + +E + S P Sbjct: 166 AIFIMDGVTSLGGDEVKVDEWGVDIAIVGSQKCLAAPPGMSAVSVSEKAFEAINSMKKRP 225 Query: 196 RNSILSLLDLKP-SPDGAAPLRVPPNIPTLEA-RALIAALTEVEEEGLQQVIKRHERXXX 253 + DLK G P P P L A+ AL V+EEG++ IKRH Sbjct: 226 YYN-----DLKAYKKSGDKPRPETPYTPALPLFYAMQEALHIVKEEGMEVRIKRHRALSE 280 Query: 254 XXXXXXXXLNLE--PWQKDSRHYSTLTTTIRI-SGKEGLLIRQPTG----IVAPGDGELF 306 +N+E P + YS T ++ +G +G ++ I+A G L Sbjct: 281 AVRAAAGAMNIEMFPQLNEYSKYSNTVTAMKAPAGVDGEDVKNDMKKRGVIIAGGQEHLK 340 Query: 307 GQLLRINHFGANASRD 322 G++ RI + G +RD Sbjct: 341 GKIFRIGNMGNVTARD 356 >CAC0221 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 377 Score = 106 bits (265), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 96/348 (27%), Positives = 156/348 (44%), Gaps = 22/348 (6%) Query: 16 FNTLTGLLSKLLYTQQPPVIIPGEAILGIEAVAAGIAAPGRTFVNVVTGPYGRLFGQWLE 75 + T + K L T+ I+ GE ILG+EA A + G + + G +G F +++ Sbjct: 39 YKKTTEKIGKFLKTKNEVRILSGEGILGLEAACASLTEKGDRVLVIENGIFGEGFADFVK 98 Query: 76 RGGATVVEVKVPFDEVAKPDVIASAIEQYQPDALSF-----VHAEVVTGGSNPAQDIFKL 130 G F K + +E++ F VH + +G N I KL Sbjct: 99 MYGGE----PFFFRGNKKCSINIEELERFLDSDSEFKYATVVHCDTPSGMLNNVGAICKL 154 Query: 131 AQKYKLITVSDSVSAVGGEALNVDEWGVDFAVIGAQKALAGPNGVSSVSISPRGWEFLES 190 +K ++TV DSVSA+GGE L VDEW +D + G+QK ++ P G++ VSIS + ++ Sbjct: 155 LKKKGIMTVVDSVSAMGGEELKVDEWDIDIVIGGSQKCISAPPGLTIVSISDDAFSAMK- 213 Query: 191 NPAAPRNSILSLLDLKPSPDGAAPLRVPPNIPTLEARALIAALTEVEEEGLQQVIKRHER 250 N P S +L D P P + AL A+ + EE + +I RH++ Sbjct: 214 NRKTPIASFYC--NLLVWEDYYNKKWFPYTPPISDIVALRKAVENILEE--KDIIARHDK 269 Query: 251 XXXXXXXXXXXLNLEPWQKDSRHYSTLTTTIRI---SGKEGLL--IRQPTGIVAPGDGE- 304 LE + +D +S T I++ + +L +++ ++ G + Sbjct: 270 IASATRAAVVLGGLEIYIEDG--FSNTVTVIKVPHGMNDDKILSYMKENYNVMIAGAFDY 327 Query: 305 LFGQLLRINHFGANASRDSVEKAIAALARLLGQEPGEALQAVREVWGE 352 L G++LRI H G NA D V + AL R L E + + +V+ E Sbjct: 328 LKGKVLRIGHMGENAYVDKVSYTLFALQRTLEHYGFEFKRDMVKVFLE 375 >PAE3447 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 384 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 90/330 (27%), Positives = 153/330 (46%), Gaps = 15/330 (4%) Query: 10 PLSLAEFNTLTGLLSKLLYTQQPPVII-PGEAILGIEAVAAGIAAPGRTFVNVVTGPYGR 68 P E+ + LL LL Q V I GEA+LG+EA A PG + + G YG Sbjct: 37 PQFFQEYKQVIELLRPLLGAWQSRVYIWVGEAMLGLEAALANAVRPGTKVLVIDNGVYGS 96 Query: 69 LFGQWLERGGATVVEVKVPFDEVAKPDVIASAIEQYQP-DALSFVHAEVVTGGSNPAQDI 127 F ++ G + + + A P I +++ + + ++ VH + + N ++I Sbjct: 97 GFADLVKMYGGNPITPGLSWRRSADPSAIERILDKEKDVEVVTLVHCDTPSTVYNNLREI 156 Query: 128 FKLAQKYKLITVSDSVSAVGGEALNVDEWGVDFAVIGAQKALAGPNGVSSVSISPRGWE- 186 K+ + + D+VS++ + + VD+WGVD + G+QKAL P G++ +SIS R W+ Sbjct: 157 AKIVSSHGAYLIVDAVSSIAADEIRVDDWGVDVLIGGSQKALNAPPGLTILSISKRAWDR 216 Query: 187 FLE-SNPAAPRNSILSLLDLKPSPDGAAPLRVPPNIPTLEARALIAALTEVEEEGLQQVI 245 LE + P+ N + L+ P + + AL +L + EG + V Sbjct: 217 ALEVARPSFYMNYQIWEEWLEKGG-------FPYTMSDVLIYALKESLQRIHNEGFESVF 269 Query: 246 KRHERXXXXXXXXXXXLNLEPWQK---DSRHYSTLTTTIRISGKEGLLIRQPTGIVAPGD 302 +RH+ L LEP+ + D+ +T T S + I + G++ G Sbjct: 270 QRHKAARAAFRAGIEALGLEPYIERVDDTCPTATAFKTPLPSPELRRFIWEKYGVLFAGS 329 Query: 303 -GELFGQLLRINHFGANASRDSVEKAIAAL 331 G L +++RI+H G AS DS+ +I+ L Sbjct: 330 WGPLEREIMRISHMGIQASADSIAASISIL 359 >DR1350 [E] COG0075 Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Length = 390 Score = 86.3 bits (212), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 67/220 (30%), Positives = 100/220 (45%), Gaps = 9/220 (4%) Query: 27 LYTQQP---PVIIPGEAILGIEAVAAGIAAPGRTFVNVVTGPYGRLFGQWLERGGATVVE 83 +Y +P ++ G LG+EA A + G + G +GR + R GA V Sbjct: 54 MYGTEPEGFTALLAGTGSLGMEAGFANLVEAGDEVLVCANGSFGRRMAEMAARYGAKVRL 113 Query: 84 VKVPFDEVAKPDVIASAIEQYQPDALSFVHAEVVTGGSNPAQDIFKLAQKYKLITVSDSV 143 V P E KP +A+ ++ ++ VH E TG NP +I +L + + D+V Sbjct: 114 VTAPLGEAIKPADVAAHLDD-DISMVAVVHGETSTGVLNPVPEIAELVRGSGALLTVDAV 172 Query: 144 SAVGGEALNVDEWGVDFAVIGAQKALAGPNGVSSVSISPRGWEFLESNPAAPRNSI-LSL 202 + G E ++ +WGVD+A GAQK L+ P G++ V+IS R + S AA R L Sbjct: 173 TTAGMEPFHMQQWGVDYAYTGAQKCLSAPPGLAPVAISERAF----SRFAARRTQTPLWY 228 Query: 203 LDLKPSPDGAAPLRVPPNIPTLEARALIAALTEVEEEGLQ 242 D + D +P A AAL EEGL+ Sbjct: 229 CDFEGLRDYWVNHTYHHTVPVNLHFAFHAALRAALEEGLE 268 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,740,594 Number of extensions: 1041482 Number of successful extensions: 1719 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1704 Number of HSP's successfully gapped: 16 Length of query: 352 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 245 Effective length of database: 26,037,693 Effective search space: 6379234785 Effective search space used: 6379234785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)