BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0002.p (391 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 106 5e-23 SSO1811 [Q] COG1228 Imidazolonepropionase and related amidohydro... 99 1e-20 CC2672 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 82 1e-15 CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 81 2e-15 BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 75 2e-13 >CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 428 Score = 106 bits (265), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 108/379 (28%), Positives = 160/379 (42%), Gaps = 33/379 (8%) Query: 28 IVIQDGRFTSIIE---AEPQLEEPAHPDQRLWISPGIIDLHTHLAWTDFDHDD-QLKRDS 83 +V+++G+ T I++ AEP + D ++ PG+ID H HL + Q S Sbjct: 48 LVLENGKVTRILDGYVAEPGGKVVDLKDS--FVLPGLIDSHVHLTGQQNPNGRLQAVTQS 105 Query: 84 REVEAMQADAFA-ATLRTGVTTARDAGGILPS--TIRHLVQH--YQQPLRVQTSSDM--- 135 AM FA TL G TT + G + +R V+ P + + S + Sbjct: 106 PSDSAMVGAGFARKTLMAGFTTVANLGANSQAIFALREGVKRGDVAGPRILASGSSISVH 165 Query: 136 ------------LXXXXRGPADARGVQHLGERMAQIFGTGAGWIKIMATGGLGAPTEKVL 183 + R + G + + GA IKI ATGG+ + T L Sbjct: 166 GGHGDINGYSEDVMHALRPESVCSGADDCRRAVREQIWLGADLIKITATGGVLSNTAAGL 225 Query: 184 EPNFSEEEFAFIVRNAHSNHKKVLVHTWGGVTIDWSIVAGVESIEHGMFLSEEQAGRLAE 243 FS+ E A IV +AHS +KV H GG I+ + AG +SIEHG +L E + + Sbjct: 226 AKQFSDAELAAIVESAHSMGRKVTAHAHGGDGINAFLKAGGDSIEHGTYLDNEGIALMKK 285 Query: 244 SAVAFVPTTS----IYRIAADPKGVLELNPVICDRXXXXXXXXXXXIGYAKKAGVRIGFG 299 S VPT +YRIA+ P P + + A GV++ FG Sbjct: 286 SGAYLVPTLMAGDFVYRIASGPNNY--FTPAQTAKALEAGPKMLDMVRRAHADGVKVAFG 343 Query: 300 TDYATPALHGYNLQELDTLMDYGLTRAEAWKSATESAAGILGSGSELGRIAEGYIADAVI 359 TD A HG N E L+ G+T EA ++AT +AA +E+G + G AD + Sbjct: 344 TDSGVSA-HGDNAGEFALLVKAGMTPLEAIQTATVNAADHFSLSNEIGSLTPGKAADLIA 402 Query: 360 YNADPYQARNAEQLRNSIV 378 DP + Q S++ Sbjct: 403 VKGDPLKDVTELQRVTSVI 421 >SSO1811 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 396 Score = 99.4 bits (246), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 99/358 (27%), Positives = 157/358 (43%), Gaps = 53/358 (14%) Query: 53 QRLWISPGIIDLHTH---------LAWTDFDHDDQLKRDSREVEAMQADAFAATLRTGVT 103 Q +I PG++D H H L+W + D R +R++E + LR+G T Sbjct: 44 QANFIMPGLVDAHLHFFGVEEDNVLSWNIVNEIDVAIRSTRDMERL--------LRSGFT 95 Query: 104 TARDAGGILPSTIRHLVQHYQQPLRVQTSSDMLXXXXRGPADARGVQ-HLGERMAQIFGT 162 RD G + + +L + + +S G D + + + +R++ F Sbjct: 96 LVRDLGSKVAVRLDYLQRRGEILGPTVIASGYSLAITGGNDDPKDLPIDIAQRLSYSFYC 155 Query: 163 ----------------GAGWIKIMATGGLGAPTEKVLEPNFSEEEFAFIVRNAHSNHKKV 206 GA IK+ A+G + K+L P F+ +E IV +H KV Sbjct: 156 DSPYECRKAVRMAIRQGARVIKVYASGAF-SQGGKIL-PGFALDELKAIVDESHRFGLKV 213 Query: 207 LVHTWGGVTIDWSIVAGVESIEHGMFLSEEQAGRLAESAVAFVPTTSIYRI---AADPKG 263 H +G I SI+AGV++IEHG+ L +E A + E + ++PT + Y + DP+ Sbjct: 214 ASHAYGKEAIMNSILAGVDTIEHGLGLDDETASMIKERGICYIPTLATYEVPFEVRDPE- 272 Query: 264 VLELNPVICDRXXXXXXXXXXXIGYAKKAGVRIGFGTDY--ATPALHGYNLQELDTLMDY 321 V R I A GV+I GTDY + HG N +E L Y Sbjct: 273 ------VRIYREELVKRHFSEDIRIAVSHGVKIATGTDYVGSKKRPHGQNYRESVLLSRY 326 Query: 322 GLTRAEAWKSATESAAGILGSGSELGRIAEGYIADAVIYNADPYQARNAEQLRNSIVS 379 ++ E +++T A+ L G ++G I EGY AD ++ DP R+ E L+ + VS Sbjct: 327 -MSNLEVLRASTSIASECL--GVKVGYIREGYKADLIVLKDDP--TRDIENLKPNNVS 379 >CC2672 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 429 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 100/379 (26%), Positives = 154/379 (40%), Gaps = 52/379 (13%) Query: 23 GKRFD---IVIQDGRFTSIIEAEPQLEEPAHPDQRLWIS--PGIIDLHTHL-AWTDFDHD 76 GK D +++ DGR TSI + + A ++ PG+ID+H HL + + Sbjct: 43 GKYVDNPLVIVTDGRITSIGKKGDAVPAGATAVDLPGVTLLPGLIDMHVHLDSLAEVGGY 102 Query: 77 DQLKRDSREVEAMQADAFAATLRTGVTTARDAG---------------GILP-------- 113 + L+ R +Q TL G TT R+ G G +P Sbjct: 103 NSLEYSDRFWSVVQTANAKKTLEAGFTTVRNVGAADYDDVGLREAIDAGYVPGPRIVTAA 162 Query: 114 ----STIRHLVQHYQQPLRVQTSSDMLXXXXRGPADAR-GVQHLGERMAQIFGTGAGWIK 168 +T H + P Q + P +AR V+ L + AQ+ IK Sbjct: 163 ISFGATGGHCDSTFFPPSMDQKNP----FNSDSPDEARKAVRTLKKYGAQV-------IK 211 Query: 169 IMATGGLGAPTEKVLEPNFSEEEFAFIVRNAHSNHKKVLVHTWGGVTIDWSIVAGVESIE 228 I ATGG+ + + + + EE +V AH KV H G I ++ AGV++IE Sbjct: 212 ICATGGVFSRGNEPGQQQLTYEEMKAVVDEAHMAGIKVAAHAHGASGIREAVRAGVDTIE 271 Query: 229 HGMFLSEEQAGRLAESAVAF---VPTTSIYRIAADPKGVLELNPVICDRXXXXXXXXXXX 285 H + +E + F + T + GVLE N + Sbjct: 272 HASLVDDEGIKLAVQKGAYFSMDIYNTDYTQAEGKKNGVLEDN---LRKDRDIGELQREN 328 Query: 286 IGYAKKAGVRIGFGTDYATPALHGYNLQELDTLMDYGLTRAEAWKSATESAAGILGSGSE 345 A KAGV++ +GTD A HG N ++ ++ YG T +A +SAT +AA LG + Sbjct: 329 FRKALKAGVKMVYGTD-AGIYPHGDNAKQFAVMVRYGATPLQAIQSATLTAAEALGRSKD 387 Query: 346 LGRIAEGYIADAVIYNADP 364 +G++A G D + DP Sbjct: 388 VGQVAVGRYGDMIAVAGDP 406 >CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 431 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 98/346 (28%), Positives = 148/346 (42%), Gaps = 50/346 (14%) Query: 55 LWISPGIIDLHTHLAWTDFDHDDQLKRDSREVEAMQADAFAA----TLRTGVTTARDAG- 109 L + PG+ID+HTHL D + + + A QA A TL G T+ RD G Sbjct: 77 LTVLPGLIDMHTHL--VDDEQSENIALPLLRSAAQQAYIGAGHARTTLMAGFTSVRDVGT 134 Query: 110 --GILPSTIRHLVQHYQQP-LRVQTSSDMLXXXXRG------------PADAR-GV---- 149 + + +R + P R+ + + G PAD R GV Sbjct: 135 WRALGDAALRDAITEGLVPGPRMSVAGAYVTAPGGGGEITGVAPDVTIPADMRRGVVEDA 194 Query: 150 QHLGERMAQIFGTGAGWIKIMATGGLGAPTEKVLEPNFSEEEFAFIVRNAHSNHKKVLVH 209 + ++ + GA +IK++ATG + + + SEEE V A V H Sbjct: 195 ADVHRKVRALLVGGADFIKLIATGAVLTEGTEPGQLELSEEEIRAAVEEAAKRGTYVTAH 254 Query: 210 TWGGVTIDWSIVAGVESIEHGMFLSEEQAGRLAESAVAFVPTTSIY---------RIAAD 260 G I ++ AGV SIEHG + +E L ++ AF+ IY R Sbjct: 255 AHGAEGIKIAVRAGVRSIEHGSLIDDEGIA-LMKAKGAFL-VADIYNGDFIDTYGREHGW 312 Query: 261 PKGVLELNPVICDRXXXXXXXXXXXIGYAK--KAGVRIGFGTDYATPALHGYNLQELDTL 318 P G++ N D G+ K KAGV+I +GTD A HG+N +++ + Sbjct: 313 PAGMIRKNRETTDAQRE---------GFRKAVKAGVKIAYGTD-AGVYPHGWNARQMPYM 362 Query: 319 MDYGLTRAEAWKSATESAAGILGSGSELGRIAEGYIADAVIYNADP 364 + YG+T +A +SAT AA ++G ++G A G AD + DP Sbjct: 363 VRYGMTPMQAIQSATTVAAELMGKTGQVGCHAPGCYADLIAVAGDP 408 >BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 394 Score = 75.1 bits (183), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 83/341 (24%), Positives = 136/341 (39%), Gaps = 38/341 (11%) Query: 57 ISPGIIDLHTHLAWTDFDHDDQLKRDSREVEAMQADAFAAT---------LRTGVTTARD 107 + PG+ID+H HL D + DAF + LR G+TT R+ Sbjct: 53 VLPGLIDVHVHLGM-------DCSVDPFDTMENTEDAFTSFIAYKQGLQFLRAGITTVRN 105 Query: 108 AGGILPSTIRHLVQHYQQ---------PLRVQTSSDMLXXXXRGP---ADARGVQHLGER 155 G T ++ Y++ P ++ G + G + + Sbjct: 106 LG-----TKNNVDIIYKKAVESGLVIGPRVYAAGQPLIMTGGHGQPMGTEVDGKDEVIKA 160 Query: 156 MAQIFGTGAGWIKIMATGGLGAPTEKVLEPNFSEEEFAFIVRNAHSNHKKVLVHTWGGVT 215 Q GA IKIMATGG+ +F+ +E I + A H G Sbjct: 161 TRQRIKEGADLIKIMATGGVLTKGNHPGAVHFNVDELKCICQEAAKASLTTAAHAIGLNG 220 Query: 216 IDWSIVAGVESIEHGMFLSEEQAGRLAESAVAFVPTTSIYRIAADPKGVLELNPVICDRX 275 + +I AGV +IEHG +L +E + + + PT + K + + D+ Sbjct: 221 VKNAIKAGVNTIEHGYYLDKETVDTMRDLGIYLTPTLLAPTLLQ--KNSEHIPQHMIDKI 278 Query: 276 XXXXXXXXXXIGYAKKAGVRIGFGTDYATPALH-GYNLQELDTLMDYGLTRAEAWKSATE 334 A + GV+I GTD TP + G ++EL +++ G+T A+A +SAT Sbjct: 279 EEIEKEHRKSFRLALERGVKIIAGTDAGTPFNNPGQLVEELKIMLEEGMTTAQAIESATY 338 Query: 335 SAAGILGSGSELGRIAEGYIADAVIYNADPYQARNAEQLRN 375 A L S +G + E +AD ++ +P + + L+N Sbjct: 339 LGAKCLKVDSLIGSLEEQKLADLIVVKGNPLE--DISNLKN 377 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,651,400 Number of extensions: 1111525 Number of successful extensions: 2120 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 2108 Number of HSP's successfully gapped: 10 Length of query: 391 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 283 Effective length of database: 25,922,359 Effective search space: 7336027597 Effective search space used: 7336027597 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)