BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0004.p (378 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGpA781 [E] COG0411 ABC-type branched-chain amino acid transport... 204 2e-52 AGpA786 [R] COG0431 Predicted flavoprotein 204 2e-52 AGpA780 [E] COG0559 Branched-chain amino acid ABC-type transport... 204 2e-52 lin0218 [G] COG1175 ABC-type sugar transport systems, permease c... 201 1e-51 BH1865 [G] COG1175 ABC-type sugar transport systems, permease co... 191 1e-48 >AGpA781 [E] COG0411 ABC-type branched-chain amino acid transport systems, ATPase component Length = 373 Score = 204 bits (520), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 12/284 (4%), Positives = 45/284 (15%) Query: 95 LKPILFTLPAMVPFVLFWLAPLLYVLYLSFTEWDFMSPEKTFVGLQNYADLFSNPAFYKA 154 + + + S + + Sbjct: 39 MTRTIRWGVLGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTARFGIARSYGSYE 98 Query: 155 LRVTVLFCAGSVXXXXXXXXXXXXXMNRKLKGSALYQVLLFSPWVTPTVAVSIVWSWIYE 214 + + L + + + + Sbjct: 99 DLLADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQARELATAQKAASLP 158 Query: 215 PEVGLANTVLDVFGLEKIGWLQDPKWALVGVLLVTIWKSVGWAMIFYLVALRNVPSDLLE 274 + + + + + + + V + + Sbjct: 159 VAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLDDPGNVRNQVDIGGGGLFDVG 218 Query: 275 AGDLDGASAGQKFFRITLPLISPTTLFLFVVQLIQALQAYDQINVLTQGGPSGSTRTLLY 334 ++ A + + +L L A+ + L + + T Sbjct: 219 CYAINTARFLFDAEPLRAIALMERDPVFGTDRLTSGLLAFPEGKQLAFTCSTQLSLTQKV 278 Query: 335 LYYQSAFESFQIGEASSVAVVLVTICMLLSVFSFGVSRRTTHYQ 378 + ++ A + G R Sbjct: 279 TVLGTRGRLEIPIPFNAPADEPTVLIFDDGRDLAGGGREEIMIG 322 >AGpA786 [R] COG0431 Predicted flavoprotein Length = 323 Score = 204 bits (520), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 107/284 (37%), Positives = 165/284 (58%) Query: 95 LKPILFTLPAMVPFVLFWLAPLLYVLYLSFTEWDFMSPEKTFVGLQNYADLFSNPAFYKA 154 L P LF P M+ LF P+L L LSF EW+F+SP FVG +NY L ++ + + Sbjct: 39 LTPYLFVAPLMIFIALFTYIPILASLNLSFREWNFLSPNMPFVGFRNYEQLLASRDLWNS 98 Query: 155 LRVTVLFCAGSVXXXXXXXXXXXXXMNRKLKGSALYQVLLFSPWVTPTVAVSIVWSWIYE 214 L VT LF SV + R+ L + LF P VT TV++++V+SW++ Sbjct: 99 LWVTTLFAVLSVPLRLGLALVIAAYLVRESVHVRLLRGALFLPSVTSTVSIAVVFSWVFS 158 Query: 215 PEVGLANTVLDVFGLEKIGWLQDPKWALVGVLLVTIWKSVGWAMIFYLVALRNVPSDLLE 274 + G+AN +L FGL K+ WLQ+P AL ++ V WK +G+ ++ Y+ L+ +P +L + Sbjct: 159 TDYGMANALLGTFGLGKVQWLQNPHLALWVLIFVNTWKQIGYDIVIYIAGLQAIPRELYD 218 Query: 275 AGDLDGASAGQKFFRITLPLISPTTLFLFVVQLIQALQAYDQINVLTQGGPSGSTRTLLY 334 A +DG F RIT+PL+ PTT FL V+ +I+A Q + +NV+T+GGP+G+T L+ Sbjct: 219 AAAVDGGKRLHVFRRITMPLVMPTTYFLLVISVIEAFQVFTIVNVMTKGGPAGATDMLVS 278 Query: 335 LYYQSAFESFQIGEASSVAVVLVTICMLLSVFSFGVSRRTTHYQ 378 L Y+ F F IG S++AV+L + + L++ + R HY+ Sbjct: 279 LLYEIGFVLFDIGRGSALAVLLFILLVGLAILKSRIIGRKVHYE 322 >AGpA780 [E] COG0559 Branched-chain amino acid ABC-type transport system, permease components Length = 373 Score = 204 bits (520), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 12/284 (4%), Positives = 45/284 (15%) Query: 95 LKPILFTLPAMVPFVLFWLAPLLYVLYLSFTEWDFMSPEKTFVGLQNYADLFSNPAFYKA 154 + + + S + + Sbjct: 39 MTRTIRWGVLGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTARFGIARSYGSYE 98 Query: 155 LRVTVLFCAGSVXXXXXXXXXXXXXMNRKLKGSALYQVLLFSPWVTPTVAVSIVWSWIYE 214 + + L + + + + Sbjct: 99 DLLADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQARELATAQKAASLP 158 Query: 215 PEVGLANTVLDVFGLEKIGWLQDPKWALVGVLLVTIWKSVGWAMIFYLVALRNVPSDLLE 274 + + + + + + + V + + Sbjct: 159 VAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLDDPGNVRNQVDIGGGGLFDVG 218 Query: 275 AGDLDGASAGQKFFRITLPLISPTTLFLFVVQLIQALQAYDQINVLTQGGPSGSTRTLLY 334 ++ A + + +L L A+ + L + + T Sbjct: 219 CYAINTARFLFDAEPLRAIALMERDPVFGTDRLTSGLLAFPEGKQLAFTCSTQLSLTQKV 278 Query: 335 LYYQSAFESFQIGEASSVAVVLVTICMLLSVFSFGVSRRTTHYQ 378 + ++ A + G R Sbjct: 279 TVLGTRGRLEIPIPFNAPADEPTVLIFDDGRDLAGGGREEIMIG 322 >lin0218 [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 201 bits (512), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 100/268 (37%), Positives = 164/268 (61%), Gaps = 3/268 (1%) Query: 86 MTRSKVISGLKPILFTLPAMVPFVLFWLAPLLYVLYLSFTEWDFMSPEKTFVGLQNYADL 145 MTR K + L +F LP ++ F++F P+L L +SFT+WD +S F G +NY L Sbjct: 4 MTRKKTLFWL--CIFLLPNLLGFLIFIAIPILGSLGISFTDWDLVSTIH-FTGFENYKRL 60 Query: 146 FSNPAFYKALRVTVLFCAGSVXXXXXXXXXXXXXMNRKLKGSALYQVLLFSPWVTPTVAV 205 F++P F+K+ R T+ F G + +N+K+K ++ + F P +T VAV Sbjct: 61 FNDPEFWKSFRNTITFIIGYLPSVMILGLACALMLNQKIKLRPFFRAVYFLPVITSWVAV 120 Query: 206 SIVWSWIYEPEVGLANTVLDVFGLEKIGWLQDPKWALVGVLLVTIWKSVGWAMIFYLVAL 265 S+VW W++ P GL N L + G+ WL DP+ A++ V++ + WK +G+ M+ +L L Sbjct: 121 SLVWKWLFNPSYGLINYFLSLIGIAGPNWLTDPQTAMIAVIITSAWKDIGFVMVLFLGGL 180 Query: 266 RNVPSDLLEAGDLDGASAGQKFFRITLPLISPTTLFLFVVQLIQALQAYDQINVLTQGGP 325 +N+ EA ++DGAS ++ F IT+PL++PTT F+ ++ LI + Q +DQ+ ++T+GGP Sbjct: 181 QNISPSYYEAANMDGASKLRQLFSITIPLLTPTTFFVTIISLINSFQVFDQVMIMTEGGP 240 Query: 326 SGSTRTLLYLYYQSAFESFQIGEASSVA 353 SG+T TL+ Y AF F++G AS+++ Sbjct: 241 SGATMTLVQNIYNHAFRYFEMGYASAMS 268 >BH1865 [G] COG1175 ABC-type sugar transport systems, permease components Length = 309 Score = 191 bits (486), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 99/279 (35%), Positives = 164/279 (58%), Gaps = 1/279 (0%) Query: 99 LFTLPAMVPFVLFWLAPLLYVLYLSFTEWDFMSPEKTFVGLQNYADLFSNPAFYKALRVT 158 LF P ++ F +F L P+LY +Y+SFT++D + E + G NY LF ++ Sbjct: 32 LFIAPPIIGFAIFALGPMLYSIYVSFTDFDLYN-EPVWTGADNYYRLFVTDDLFRKTVFN 90 Query: 159 VLFCAGSVXXXXXXXXXXXXXMNRKLKGSALYQVLLFSPWVTPTVAVSIVWSWIYEPEVG 218 + A + +N+K+KG AL++ F P V+ VA++++W WI+ + G Sbjct: 91 TFYAALGIPIGMAVSLGIAVALNQKVKGIALFRTAFFLPAVSSVVAITLLWRWIFNADFG 150 Query: 219 LANTVLDVFGLEKIGWLQDPKWALVGVLLVTIWKSVGWAMIFYLVALRNVPSDLLEAGDL 278 L N +L+ G+ GWL D KWA+ +++ +W +G MI YL AL+ V L EA D+ Sbjct: 151 LLNIMLNYVGIHGPGWLSDEKWAMPAMIIQGVWGGLGINMILYLAALQGVNPALYEAADI 210 Query: 279 DGASAGQKFFRITLPLISPTTLFLFVVQLIQALQAYDQINVLTQGGPSGSTRTLLYLYYQ 338 DG +A QKF IT+P ISPTT F+ + I ALQ + + ++T+GGP+ ST T++Y + Sbjct: 211 DGGNAWQKFIHITVPSISPTTFFILITSTIGALQDFQRFMIMTEGGPNYSTTTVVYYLFL 270 Query: 339 SAFESFQIGEASSVAVVLVTICMLLSVFSFGVSRRTTHY 377 +AF ++G AS++A VL I +++++ +F ++++ HY Sbjct: 271 NAFRYMEMGYASAMAWVLGIIILIITIINFKLAKKWVHY 309 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,415,605 Number of extensions: 938550 Number of successful extensions: 3462 Number of sequences better than 1.0e-05: 170 Number of HSP's gapped: 3202 Number of HSP's successfully gapped: 171 Length of query: 378 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 270 Effective length of database: 25,922,359 Effective search space: 6999036930 Effective search space used: 6999036930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)