BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0002.p (451 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl1352 [G] COG1653 ABC-type sugar transport system, periplasmic... 107 3e-23 Rv2833c [G] COG1653 ABC-type sugar transport system, periplasmic... 104 3e-22 AGpA775 [E] COG0410 ABC-type branched-chain amino acid transport... 101 2e-21 AGpA778 [E] COG4177 ABC-type branched-chain amino acid transport... 101 2e-21 mll3503 [G] COG1653 ABC-type sugar transport system, periplasmic... 94 4e-19 >Cgl1352 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 438 Score = 107 bits (268), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 104/388 (26%), Positives = 166/388 (42%), Gaps = 61/388 (15%) Query: 75 LIQKFNASQQEVIVKGI-VQADYSETEQKLQAAIATGQVPAAVLSSNMDW---ARKGYFA 130 LI +F A ++ V+ + A+Y+E QK AA++ G +P V+ S+ +W A G Sbjct: 64 LISRFEAENPDLKVQLVDAGANYAEVSQKFNAALSGGDLPDLVVLSDTEWFNFAINGATV 123 Query: 131 SMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRKDAFEKSGIDASQL 190 ++DE +A + + + +V + +G Y LP +T + YY KD + K+G++ Sbjct: 124 NIDE-VAKRNNIDTSTYVDSLFNDYSHEGGHYGLPFARSTVLFYYNKDLWAKAGLEDRGP 182 Query: 191 KTWEDLAAAAKKM--------AVQDGGKTTFFGWE---PMWGSGNMIDAVMSKGGSILSG 239 ++WE+ + K+ A G T + W PMW S GG+ G Sbjct: 183 ESWEEFSEWGPKLQEAMDSGFAHGWGDATNYLSWTFEGPMW----------SLGGNYSEG 232 Query: 240 DGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTID-DVMKGQAAGYTGS 298 +++T +PE + + + + E + T+ DV A G GS Sbjct: 233 WESRLT--TPETIRAVEWLKSTVDEG---------------FATVSTDVTNEFATGLIGS 275 Query: 299 SGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMA------GIPAKAGDAEKQAAMKWLT 352 Q D S V+ W P E GIP+ + + A+K++ Sbjct: 276 C-IQSTGDLSSVAGAASFDWGVAALPNPTGEGACPTGGAGLGIPSGISEQRQDNALKFID 334 Query: 353 YFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQD---- 408 + TN+ NT +WS TGY+ VR+ A DP +F E NP V + Q + QD Sbjct: 335 FLTNAANTGYWSRETGYVPVRKDAASDPDHAAFLEENPAYNVAVEQLPDTRS--QDNFRV 392 Query: 409 --PTGGK-ISDAL-KIAADKVQIENTPA 432 P G + I DAL KI I+ T A Sbjct: 393 LLPNGDRTIGDALEKICLTGADIDVTLA 420 >Rv2833c [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 436 Score = 104 bits (259), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 103/411 (25%), Positives = 168/411 (40%), Gaps = 42/411 (10%) Query: 58 IEFWYGLGGKLGENMESLIQKFNASQQEVIVKGI-VQADYSETEQKLQAAIATGQVPAAV 116 I+FW G+ LI +F + VK I DY E QK AA+ VP V Sbjct: 39 IDFWSSHPGQSSAAERELIGRFQDRFPTLSVKLIDAGKDYDEVAQKFNAALIGTDVPDVV 98 Query: 117 LSSNMDW---ARKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVM 173 L + W A G ++D+L Q + D+V + L + +G+ Y +P +T + Sbjct: 99 LLDDRWWFHFALSGVLTALDDLFG-QVGVDTTDYVDSLLADYEFNGRHYAVPYARSTPLF 157 Query: 174 YYRKDAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGNMIDAVMSKG 233 YY K A++++G+ ++W + ++ G + GW + ++I Sbjct: 158 YYNKAAWQQAGLPDRGPQSWSEFDEWGPELQRVVGAGRSAHGW----ANADLISWTFQGP 213 Query: 234 GSILSG---DGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKG 290 G D +T+ P + + +R IH G+G+ +D+ Sbjct: 214 NWAFGGAYSDKWTLTLTEPATIAAGNFYRNSIH----------GKGYA---AVANDIANE 260 Query: 291 QAAGYTGSS-GDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAG-----IPAKAGDAEK 344 A G S+ G L SA G A + G A A G IPAK + K Sbjct: 261 FATGILASAVASTGSLAGITASARFDFGAAPLPTGPDAAPACPTGGAGLAIPAKLSEERK 320 Query: 345 QAAMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASA 404 A+K++ + TN NTA++S TGY+ VR+SA+ D + + NP+ +V L Q H Sbjct: 321 VNALKFIAFVTNPTNTAYFSQQTGYLPVRKSAVDDASERHYLADNPRARVALDQLPHTRT 380 Query: 405 PFQD------PTGGKISDALKIAADKVQIENTPAAEALKEAQQTAQAALDK 449 QD P G +I + + + + + Q+ Q LD+ Sbjct: 381 --QDYARVFLPGGDRI---ISAGLESIGLRGADVTKTFTNIQKRLQVILDR 426 >AGpA775 [E] COG0410 ABC-type branched-chain amino acid transport systems, ATPase component Length = 373 Score = 101 bits (252), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 20/363 (5%), Positives = 56/363 (15%), Gaps = 32/363 (8%) Query: 57 TIEFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAV 116 K+ + + + ++ I + A + A Sbjct: 28 RKCSERREEKKMTRTIRWGVLGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTAR 87 Query: 117 LSSNMDWARKGYFASMDELIAAQPDFN----KEDFVQTFLKQGQVDGKQYFLPMYGTTQV 172 G + + +N T Q P+ Sbjct: 88 F---GIARSYGSYEDLLADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQ 144 Query: 173 MYYRKDAFEKSGIDA-SQLKTWEDLAAAAK----KMAVQDGGKTTFFGWEPMWGSGNMID 227 A + + + + + Sbjct: 145 ARELATAQKAASLPVAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLD------ 198 Query: 228 AVMSKGGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMR---IHSGGQGWEYWYKTI 284 + + G G + LF + G Sbjct: 199 DPGNVRNQVDIGGGGLFDVGCYAINTARFLFDAEPLRAIALMERDPVFGTDRLTSGLLAF 258 Query: 285 DDVMKGQAAGYTGSSGDQ-GDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAE 343 + + T S Q + + + + + Sbjct: 259 PEGKQLAFTCSTQLSLTQKVTV---LGTRGRLEIPIPF----NAPADEP-TVLIFDDGRD 310 Query: 344 KQAAMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPL--MQASH 401 + + + + L+ + + T + Sbjct: 311 LAGGGREEIMIGPVDQYREQADAFAEAVLSGKPLETDLGDAIANTKAIDALFRSAASGRW 370 Query: 402 ASA 404 Sbjct: 371 EEP 373 >AGpA778 [E] COG4177 ABC-type branched-chain amino acid transport system, permease component Length = 373 Score = 101 bits (252), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 20/363 (5%), Positives = 56/363 (15%), Gaps = 32/363 (8%) Query: 57 TIEFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAV 116 K+ + + + ++ I + A + A Sbjct: 28 RKCSERREEKKMTRTIRWGVLGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTAR 87 Query: 117 LSSNMDWARKGYFASMDELIAAQPDFN----KEDFVQTFLKQGQVDGKQYFLPMYGTTQV 172 G + + +N T Q P+ Sbjct: 88 F---GIARSYGSYEDLLADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQ 144 Query: 173 MYYRKDAFEKSGIDA-SQLKTWEDLAAAAK----KMAVQDGGKTTFFGWEPMWGSGNMID 227 A + + + + + Sbjct: 145 ARELATAQKAASLPVAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLD------ 198 Query: 228 AVMSKGGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMR---IHSGGQGWEYWYKTI 284 + + G G + LF + G Sbjct: 199 DPGNVRNQVDIGGGGLFDVGCYAINTARFLFDAEPLRAIALMERDPVFGTDRLTSGLLAF 258 Query: 285 DDVMKGQAAGYTGSSGDQ-GDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAE 343 + + T S Q + + + + + Sbjct: 259 PEGKQLAFTCSTQLSLTQKVTV---LGTRGRLEIPIPF----NAPADEP-TVLIFDDGRD 310 Query: 344 KQAAMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPL--MQASH 401 + + + + L+ + + T + Sbjct: 311 LAGGGREEIMIGPVDQYREQADAFAEAVLSGKPLETDLGDAIANTKAIDALFRSAASGRW 370 Query: 402 ASA 404 Sbjct: 371 EEP 373 >mll3503 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 450 Score = 94.4 bits (233), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 98/395 (24%), Positives = 166/395 (42%), Gaps = 31/395 (7%) Query: 50 PTQGKPVTIEFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIAT 109 P +PV +FW+GL G L +++L + FNASQ++ V Q +Y T Q AA Sbjct: 23 PASAEPVKFDFWFGLSGDLARVVDTLCKNFNASQKDYEVVCTSQGNYDATLQNTIAAFRA 82 Query: 110 GQVPAAVLSSNMDWAR---KGYFASMDELIAAQPDFNKEDFVQTFLKQGQV----DGKQY 162 G+ PA V ++ A G + D+L+ + + K D+ F + G+ Sbjct: 83 GKQPAVVQVYDVGTATMMLSGAYKPADKLM--EENGYKIDYADYFPGIARYYATSKGEML 140 Query: 163 FLPMYGTTQVMYYRKDAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFG------- 215 P +T +MY+ KDAF K G KTWED+ A K A++D G Sbjct: 141 SFPFNSSTPLMYWNKDAFAKIG-KTEAPKTWEDVGADLK--ALKDAGYECPMAINISANE 197 Query: 216 -WEPMWGSGNMIDAVMSKGGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGG 274 W+ M + + ++ + G ++ ++ ++V+ +KW ++ +++I S Sbjct: 198 SWQLMEQFSALHNQPIATKNNGYDGLDARLEVNKTKFVQYVTDLKKW-YDAGLIKIKSKD 256 Query: 275 QGWEYWYKTIDDVMKGQAAGYTGSSGDQGDLDFSIVSAM-----EQPGWAGVGEGKPVAE 329 G + + G S GD G + + M E P +AG + Sbjct: 257 LGQD----MVQAFASGTCQVILTSVGDHGTVGRTQKEGMKWDVAELPVYAGTERKNSLVG 312 Query: 330 ALMAGIPAKAGDAEKQAAMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETN 389 + + DAE + A +L + + + FWS NTGYI V +S F + Sbjct: 313 GASLWVLSGKSDAEYKGAAAFLNFIHDPKTALFWSTNTGYIPVTKSGFDYMKGQGFYDKA 372 Query: 390 PQIKVPLMQAS-HASAPFQDPTGGKISDALKIAAD 423 P + AS AS P + G ++ + +I A+ Sbjct: 373 PYKGREVAIASLTASEPTEITRGVRLGNFTQIRAE 407 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.129 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,782,364 Number of extensions: 1307257 Number of successful extensions: 2809 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 2771 Number of HSP's successfully gapped: 47 Length of query: 451 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 341 Effective length of database: 25,691,691 Effective search space: 8760866631 Effective search space used: 8760866631 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)