BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0033.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2440 [E] COG1126 ABC-type polar amino acid transport system, ... 340 1e-93 BS_hisP [E] COG1126 ABC-type polar amino acid transport system, ... 325 3e-89 BS_yckI [E] COG1126 ABC-type polar amino acid transport system, ... 314 7e-86 CAC0879 [E] COG1126 ABC-type polar amino acid transport system, ... 303 2e-82 NMA1000 [E] COG1126 ABC-type polar amino acid transport system, ... 302 3e-82 >lin2440 [E] COG1126 ABC-type polar amino acid transport system, ATPase component Length = 64 Score = 340 bits (872), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%) Query: 1 MIELKQISKSFGRHQVLNHIDLTVAKGEVVVILGPSGSGKTTLLRCVNYLEKPSGGEIAI 60 M F + ++ + + K + G Sbjct: 1 MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNG 60 Query: 61 GDFKLDCRHARKKDIHQLRQRTAMVFQQYNLFRHKTALENVMEGLLIVKKLPKEEARKKS 120 Sbjct: 61 120 Query: 121 IALLEKVGLGAKLDAYPSQLSGGQQQRVGIARALALEPEVILFDEPTSALDPELVGEVLA 180 Sbjct: 121 180 Query: 181 VIRKIAKEGITMIVVTHEMGFARDVANHVVFMDGGVIVEEGTPTEVFNHPREERTKQFLK 240 Sbjct: 181 240 Query: 241 RITPELNYSI 250 Sbjct: 241 250 >BS_hisP [E] COG1126 ABC-type polar amino acid transport system, ATPase component Length = 259 Score = 325 bits (834), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 160/245 (65%), Positives = 197/245 (80%) Query: 1 MIELKQISKSFGRHQVLNHIDLTVAKGEVVVILGPSGSGKTTLLRCVNYLEKPSGGEIAI 60 MIE+K I K FG H VL I+LTV KGEVV I+GPSGSGKTT LRC+N LE+P G I+I Sbjct: 1 MIEIKNIHKQFGIHHVLKGINLTVRKGEVVTIIGPSGSGKTTFLRCLNLLERPDEGIISI 60 Query: 61 GDFKLDCRHARKKDIHQLRQRTAMVFQQYNLFRHKTALENVMEGLLIVKKLPKEEARKKS 120 D ++CR KK++H LR++TAMVFQQY+LF HKT +ENVMEGL I +K+ K++A + Sbjct: 61 HDKVINCRFPSKKEVHWLRKQTAMVFQQYHLFAHKTVIENVMEGLTIARKMRKQDAYAVA 120 Query: 121 IALLEKVGLGAKLDAYPSQLSGGQQQRVGIARALALEPEVILFDEPTSALDPELVGEVLA 180 L KVGL KL+AYPSQLSGGQ+QRVGIARALA+ P+V+LFDEPT+ALDPELVGEVL Sbjct: 121 ENELRKVGLQDKLNAYPSQLSGGQKQRVGIARALAIHPDVLLFDEPTAALDPELVGEVLE 180 Query: 181 VIRKIAKEGITMIVVTHEMGFARDVANHVVFMDGGVIVEEGTPTEVFNHPREERTKQFLK 240 V+ +I K G TMIVVTHEM FAR V++ VVFMD GVIVE+GTP EVF H +++RT+QFL+ Sbjct: 181 VMLEIVKTGATMIVVTHEMEFARRVSDQVVFMDEGVIVEQGTPEEVFRHTKKDRTRQFLR 240 Query: 241 RITPE 245 R++PE Sbjct: 241 RVSPE 245 >BS_yckI [E] COG1126 ABC-type polar amino acid transport system, ATPase component Length = 247 Score = 314 bits (805), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 150/243 (61%), Positives = 200/243 (82%), Gaps = 1/243 (0%) Query: 1 MIELKQISKSFGRHQVLNHIDLTVAKGEVVVILGPSGSGKTTLLRCVNYLEKPSGGEIAI 60 M+ +K ++KSFG +++L ID+ + KG+V+ ILGPSGSGKTTLLRC+N LE P+ GE+A Sbjct: 1 MLTVKGLNKSFGENEILKKIDMKIEKGKVIAILGPSGSGKTTLLRCLNALEIPNRGELAF 60 Query: 61 GDFKLD-CRHARKKDIHQLRQRTAMVFQQYNLFRHKTALENVMEGLLIVKKLPKEEARKK 119 DF +D + ++ DI +LR+++ MVFQ Y+LF H+TALENVMEG + V+K KEE RK+ Sbjct: 61 DDFSIDFSKKVKQADILKLRRKSPMVFQAYHLFPHRTALENVMEGPVQVQKRNKEEVRKE 120 Query: 120 SIALLEKVGLGAKLDAYPSQLSGGQQQRVGIARALALEPEVILFDEPTSALDPELVGEVL 179 +I LL+KVGL K+D YP QLSGGQQQRVGIARALA++PE++LFDEPTSALDPELVGEVL Sbjct: 121 AIQLLDKVGLKDKMDLYPFQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVGEVL 180 Query: 180 AVIRKIAKEGITMIVVTHEMGFARDVANHVVFMDGGVIVEEGTPTEVFNHPREERTKQFL 239 VI+ +A EG TM+VVTHE+ FA++VA+ V+F+DGGVIVE+G P ++F+ P+EERT++FL Sbjct: 181 KVIKDLANEGWTMVVVTHEIKFAQEVADEVIFIDGGVIVEQGPPEQIFSAPKEERTQRFL 240 Query: 240 KRI 242 RI Sbjct: 241 NRI 243 >CAC0879 [E] COG1126 ABC-type polar amino acid transport system, ATPase component Length = 243 Score = 303 bits (775), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 136/242 (56%), Positives = 191/242 (78%) Query: 1 MIELKQISKSFGRHQVLNHIDLTVAKGEVVVILGPSGSGKTTLLRCVNYLEKPSGGEIAI 60 MI+++ + K++G ++VL I L + GE+V ++GPSG+GK+T LRC+NYLE+P G + I Sbjct: 1 MIKIESLHKTYGDNEVLKGISLEIKAGEIVAVIGPSGTGKSTFLRCLNYLERPDSGTLLI 60 Query: 61 GDFKLDCRHARKKDIHQLRQRTAMVFQQYNLFRHKTALENVMEGLLIVKKLPKEEARKKS 120 GD ++D + K +++ LR++T+MVFQ YNLF++KTALENVME L +VKK+PK+EA K S Sbjct: 61 GDVRVDFENISKDEVYSLRKKTSMVFQNYNLFKNKTALENVMEALRVVKKMPKDEAEKVS 120 Query: 121 IALLEKVGLGAKLDAYPSQLSGGQQQRVGIARALALEPEVILFDEPTSALDPELVGEVLA 180 + L++VGL K ++YPS+LSGGQQQR+GIARA+ L+PE+ILFDEPTSALDPE VGEVL Sbjct: 121 LEALKQVGLLDKANSYPSRLSGGQQQRIGIARAMVLKPEIILFDEPTSALDPEWVGEVLD 180 Query: 181 VIRKIAKEGITMIVVTHEMGFARDVANHVVFMDGGVIVEEGTPTEVFNHPREERTKQFLK 240 VI+ IA +TMI+VTHEM FAR++A+ +VF+DGG I++ GTP EVF +R QF+K Sbjct: 181 VIKGIASRHLTMIIVTHEMSFAREIADKIVFLDGGKIIQYGTPEEVFEKSNNDRVNQFIK 240 Query: 241 RI 242 ++ Sbjct: 241 KL 242 >NMA1000 [E] COG1126 ABC-type polar amino acid transport system, ATPase component Length = 202 Score = 302 bits (774), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 0/246 (0%), Positives = 0/246 (0%), Gaps = 4/246 (1%) Query: 1 MIELKQISKSFGRHQVLNHIDLTVAKGEVVVILGPSGSGKTTLLRCVNYLEKPSGGEIAI 60 M + Q I + + + + + L + K +A+ Sbjct: 1 MDWSSAEGRMCMDMQSAIVIYIVLTVTGRFLSQRQRKNNRRKQLSQWRNIVKDYTAIVAV 60 Query: 61 GD---FKLD-CRHARKKDIHQLRQRTAMVFQQYNLFRHKTALENVMEGLLIVKKLPKEEA 116 A ++ L + ++ A + + ++ Sbjct: 61 IATAFAMGLALLEAALREDTVLPITSIIIGTVIVTGGWVVAWFANRKIGTNWSPIIEKTN 120 Query: 117 RKKSIALLEKVGLGAKLDAYPSQLSGGQQQRVGIARALALEPEVILFDEPTSALDPELVG 176 R++ + + L + G A ++ ++ + + Sbjct: 121 RQELVTSGIYSLVRHPLYFSGFCILIGTNIYFCSKWAWIGTGIALITILFRIPIEEKKLK 180 Query: 177 EVLAVIRKIAKEGITMIVVTHEMGFARDVANHVVFMDGGVIVEEGTPTEVFNHPREERTK 236 Sbjct: 181 240 Query: 237 QFLKRI 242 Sbjct: 241 246 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,532,843 Number of extensions: 777867 Number of successful extensions: 11775 Number of sequences better than 1.0e-05: 2200 Number of HSP's gapped: 7869 Number of HSP's successfully gapped: 2469 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)