BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0032.p (338 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2439 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 248 1e-65 BS_ytmO [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 241 1e-63 BS_ywcH [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 241 1e-63 RSc2462 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 198 9e-51 BS_yceB [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 196 4e-50 >lin2439 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 248 bits (632), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 20/335 (5%), Positives = 46/335 (13%), Gaps = 11/335 (3%) Query: 1 MAIVISVLDQSPIYPGETPEEAFQHTVKLAQLSERLGFRRFWVSEHHDSEQVAGSSPEVL 60 M + F A +L + D ++ P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 VSHLLAKTERIRVGSGGIMLQHYSPYKVAENFNVLASLAPGRVDLGVGRAPGGLPRSTQA 120 L + G + KV+ L L S + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 LQQGGAEAPSLTD-KITELEQYVHNRLAADHPLAGLKAGPIPGTPPELYVLGASVASAEI 179 LQ + + + D + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTD-----SAPAVSLLDTLDALAEYQRAGKIRY 175 Query: 180 AASLGLPYVFSLFISGDQAVALEAIRAYRSGFDTSQ-GREPQXXXXXXXXXXXXXXXXXX 238 + R + + Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLP----RIVTIQNPYSLLNRSFEVGLAEVSQYEGVELL 231 Query: 239 XXXXHKLIRIHLASGKTLTVATREQAEEFTRQSKEAYTLEEREPEITKGTKEAVREQLLA 298 + A ++ + + Sbjct: 232 AYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQM 291 Query: 299 LAGASGVTEFIVTTNIQSFDKRLRSFELLHEAFAE 333 F+ +T + + + Sbjct: 292 ALAFVRRQPFVASTLLGATTMDQLKTNIESLHLEL 326 >BS_ytmO [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 241 bits (616), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 15/329 (4%), Positives = 48/329 (14%), Gaps = 3/329 (0%) Query: 3 IVISVLDQSPIYPGETPEEAFQHTVKLAQLSERLGFRRFWVSEHHDSEQVAGSSPEVLVS 62 + S + + P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 HLLAKTERIRVGSGGIMLQHYSPYKVAENFNVLASLAPGRVDLGVGRAPGGLPRSTQALQ 122 L +T + + + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 123 -QGGAEAPSLTDKITELEQYVHNRLAADHPLAGLKAGPIPGTPPELYVLGASVASAEIAA 181 + + + + + E G Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 182 SLGLPYVFSLFISGDQAVALEAIRAYRSGFDTSQGREPQXXXXXXXXXXXXXXXXXXXXX 241 + + + I+ S ++ E Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLL--NRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 242 XHKLIRIHLASGKTLTVATREQAEEFTRQSKEAYTLEEREPEITKGTKEAVREQLLALAG 301 ++ A + ++ + + + G A Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 302 ASGVTEFIVTTNIQSFDKRLRSFELLHEA 330 + + T + + S L Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHLELSE 328 >BS_ywcH [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 241 bits (615), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 14/334 (4%), Positives = 43/334 (12%), Gaps = 5/334 (1%) Query: 3 IVISVLDQSPIYPGETPEEAFQHTVKLAQLSERLGFRRFWVSEHHDSEQVAGSSPEVLVS 62 + S + + P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 HLLAKTERIRVGSGGIMLQHYSPYKVAENFNVLASLAPGRVDLGVGRAPGGLPRSTQALQ 122 L +T + + + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 123 QGGA-EAPSLTDKITELEQYVHNRLAADHPLAGLKAGPIPGTPPELYVLGASVASAEIAA 181 Q + + + + + E G Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 182 SLGLPYVFSLFISGDQAVALEAIRAYRSGFDTS-QGREPQXXXXXXXXXXXXXXXXXXXX 240 + + + + Sbjct: 182 TAFGVMRYLHLADKHDLPR--IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 241 XXHKLIRIHLASGKTLT-VATREQAEEFTRQSKEAYTLEEREPEITKGTKEAVREQLLAL 299 + +R + K + + + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 300 AGASGVTEFIVTTNIQSFDKRLRSFELLHEAFAE 333 + T + + EL + AE Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHLELSEDVLAE 333 >RSc2462 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 198 bits (504), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 26/332 (7%), Positives = 51/332 (15%), Gaps = 3/332 (0%) Query: 2 AIVISVLDQSPIYPGETPEEAFQHTVKLAQLSERLGFRRFWVSEHHDSEQVAGSSPEVLV 61 S+ + T E AQL + + + L Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 62 SHLLAKTERIRVGSGGIMLQHYSPYKVAENFNVLASLAPGRVDLGVGRAPGGLPRSTQAL 121 + +++ N + L R Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 122 QQGG-AEAPSLTDKITELEQYVHNRLAADHPLAGLKAGPIPGTPPELYVLGASVASAEIA 180 P Y A L Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 181 ASLGLPYVFSLFISGDQAVALEAIRAYRSGFDTSQGREPQXXXXXXXXXXXXXXXXXXXX 240 L + + V ++ + + E Sbjct: 186 VMRYLHLADKHDL--PRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 241 XXHKLIRIHLASGKTLTVATREQAEEFTRQSKEAYTLEEREPEITKGTKEAVREQLLALA 300 + A + TR E+ + + R A + +A Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 301 GASGVTEFIVTTNIQSFDKRLRSFELLHEAFA 332 + L E + Sbjct: 304 STLLGATTMDQLKTNIESLHLELSEDVLAEIE 335 >BS_yceB [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 196 bits (499), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 26/313 (8%), Positives = 58/313 (18%), Gaps = 8/313 (2%) Query: 23 FQHTVKLAQLSERLGFRRFWVSEHHDSEQVAGSSPEVLVSHLLAKTERIRVGSGGIMLQH 82 + ++S + ++ +++ A V L + Q Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 83 YSPYKVAENFNVLASLAPGRVDLGVGRAPGGLPRSTQALQQGGAEAPSLTD---KITELE 139 + V S + V + + Q + ++ + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRK--NIREALHDSLKRL 121 Query: 140 QYVHNRLAADHPLAGLKAGPIPGTPPELYVLGASVASAEIAASLGLPYVFSLFISGDQAV 199 Q + L H A + A + G Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 200 ALEAIRAYRSGFDTS-QGREPQXXXXXXXXXXXXXXXXXXXXXXHKLIRIHLASGKTLTV 258 A R LA Sbjct: 182 --TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 259 ATREQAEEFTRQSKEAYTLEEREPEITKGTKEAVREQLLALAGASGVTEFIVTTNIQSFD 318 + + + TL R + + + +A G+ + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 319 KRLRSFELLHEAF 331 + S L Sbjct: 300 PFVASTLLGATTM 312 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,741,581 Number of extensions: 928038 Number of successful extensions: 3711 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 3693 Number of HSP's successfully gapped: 30 Length of query: 338 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 231 Effective length of database: 26,037,693 Effective search space: 6014707083 Effective search space used: 6014707083 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)