BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0031.p (394 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_hipO [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 404 e-112 BS_yxeP [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 400 e-111 CAC0921 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 367 e-101 L182020 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 305 1e-82 PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 290 3e-78 >BS_hipO [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 404 bits (1039), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/371 (53%), Positives = 260/371 (70%) Query: 14 LNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQDGP 73 L +++IGIRRHLHQ+PELS EEFETT +I L++ GI I L TGV A+I G +GP Sbjct: 37 LEKQLIGIRRHLHQYPELSKEEFETTAFIKKCLKEKGIQIRPTALKTGVFADIAGESEGP 96 Query: 74 VVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGKVR 133 +ALRADIDALPI+E+TGLPYAS H G HACGHDFHTAAL+GAA+ LK+ + LKGK+R Sbjct: 97 AIALRADIDALPIEEKTGLPYASKHKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIR 156 Query: 134 LLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVVEV 193 LLFQPAEE GA ++I G L+G+ AVIG+HNKPD+ VGT+G+ GPLMAA D F VE+ Sbjct: 157 LLFQPAEEAGAGATKVIEDGQLDGIDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEI 216 Query: 194 RGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIPET 253 G G+HAA+P G DPI+ AS ++ ALQ+IVSRNV+ LQSA+++V K++ G++WN+IP+T Sbjct: 217 EGKGAHAALPHNGFDPIIGASQLIVALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDT 276 Query: 254 AVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLGEE 313 V+EGT+RTF+ VR++V +RF +W GPPP+ ND + L + Sbjct: 277 VVIEGTVRTFDSEVRNQVKQRFFAVTEQISAAFSLKANVKWHSGPPPLCNDEAITGLVRD 336 Query: 314 TAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVGAA 373 A + P+PS AGEDFA+Y +PG F F GT G +WHHPAF +DE A+ + Sbjct: 337 AAHKAKLQVIDPAPSTAGEDFAYYLEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASY 396 Query: 374 FFAGLAVRVLE 384 F A R+L+ Sbjct: 397 FLYESAKRLLD 407 >BS_yxeP [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 380 Score = 400 bits (1027), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/377 (51%), Positives = 254/377 (67%), Gaps = 1/377 (0%) Query: 12 QALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDY-GLTTGVIAEIGGLQ 70 +A + ++I +RR LH+HPELS +E ETT+ I LE+ I I+D L T VIAEI G + Sbjct: 4 KAFHTRLINMRRDLHEHPELSFQEVETTKKIRRWLEEEQIEILDVPQLKTAVIAEIKGRE 63 Query: 71 DGPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKG 130 DGPV+A+RADIDALPIQE+T LP+AS G HACGHDFHTA+++G A L Q+ +LKG Sbjct: 64 DGPVIAIRADIDALPIQEQTNLPFASKVDGTMHACGHDFHTASIIGTAMLLNQRRAELKG 123 Query: 131 KVRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFV 190 VR +FQPAEE A GA++++ +G L GV A+ GMHNKPDLPVGT+G+ GPLMA+ D F Sbjct: 124 TVRFIFQPAEEIAAGARKVLEAGVLNGVSAIFGMHNKPDLPVGTIGVKEGPLMASVDRFE 183 Query: 191 VEVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNII 250 + ++G G HA++P DPI AA I++ LQS+VSRN+S+LQ+AVVS+T++ +G SWN+I Sbjct: 184 IVIKGKGGHASIPNNSIDPIAAAGQIISGLQSVVSRNISSLQNAVVSITRVQAGTSWNVI 243 Query: 251 PETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARL 310 P+ A +EGT+RTF++ R V E +W P V ND Sbjct: 244 PDQAEMEGTVRTFQKEARQAVPEHMRRVAEGIAAGYGAQAEFKWFPYLPSVQNDGTFLNA 303 Query: 311 GEETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPV 370 E AA LG+ + SP GEDFA YQ +PG FV++GT G+ EWHHPAF LDE AL V Sbjct: 304 ASEAAARLGYQTVHAEQSPGGEDFALYQEKIPGFFVWMGTNGTEEWHHPAFTLDEEALTV 363 Query: 371 GAAFFAGLAVRVLEHLS 387 + +FA LAV VLE + Sbjct: 364 ASQYFAELAVIVLETIK 380 >CAC0921 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 382 Score = 367 bits (941), Expect = e-101, Method: Compositional matrix adjust. Identities = 168/368 (45%), Positives = 242/368 (65%) Query: 16 EKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQDGPVV 75 +K+IG RR LH+HPELS +E+ETT+ I L + I ++D+ + GV+AEI G +G V Sbjct: 12 KKLIGYRRELHKHPELSMKEYETTKRIKRWLYENDIKVLDFPIEVGVVAEIEGEHEGKTV 71 Query: 76 ALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGKVRLL 135 A+RADIDALPI+E+T LP++S++ G HACGHDFHTAA++G A L +++ ++ G+VR++ Sbjct: 72 AIRADIDALPIEEKTELPFSSVNKGIMHACGHDFHTAAILGTAIILSKRKAEIYGRVRII 131 Query: 136 FQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVVEVRG 195 FQP EE KG+Q II G L V + GMHNKPDLPVGT+GI G LMA+ D F ++V G Sbjct: 132 FQPGEETGKGSQYIIEEGVLRDVDCIFGMHNKPDLPVGTIGIRSGELMASVDRFEIKVIG 191 Query: 196 AGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIPETAV 255 +G HA +P DPIV AS IV ++Q++VSR++S ++S V+S+T+ +SG +WN+I ++A Sbjct: 192 SGGHAGIPNKCIDPIVVASQIVASMQTVVSRSISPIESVVISITRFNSGTTWNVISDSAE 251 Query: 256 LEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLGEETA 315 +EGT+RTF +R ++ +W P + ND L E A Sbjct: 252 MEGTVRTFNNNIREEIRNLMERTAKGIARALGGKIEFKWFSYSPVLENDVRFKDLCIEAA 311 Query: 316 ATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSREWHHPAFNLDEAALPVGAAFF 375 G+ Y++ + GEDF+FYQ VVP FV++G G+ EWH +NL E AL V A +F Sbjct: 312 EDNGYKYVEAKRNLGGEDFSFYQTVVPSFFVWMGVDGTEEWHKANYNLKEDALMVAANYF 371 Query: 376 AGLAVRVL 383 + LA++VL Sbjct: 372 SDLALKVL 379 >L182020 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 379 Score = 305 bits (780), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 157/378 (41%), Positives = 227/378 (60%), Gaps = 4/378 (1%) Query: 12 QALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQD 71 + L ++++ IR +LH HPE+S +E ETT++I L I I+D L TG++A+IG Sbjct: 4 ENLYQELVEIRHYLHAHPEISEKEVETTKFIRQKLADWQIEIMDSQLKTGLVAKIGS--G 61 Query: 72 GPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKGK 131 P++ALRADIDALPI EETGL ++S++ G HACGHD H +L+GAA LK++E G Sbjct: 62 KPIIALRADIDALPILEETGLEFSSVNKGAMHACGHDLHMTSLLGAAKILKEKESQFSGT 121 Query: 132 VRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFVV 191 ++++FQPAEE +G+++++ +G + V+A IG HN P L G +G+ G +MAA + F + Sbjct: 122 IKIIFQPAEEIGEGSKEVLKTGLVSDVQAFIGYHNMPTLSAGVIGLREGGVMAAVERFEI 181 Query: 192 EVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNIIP 251 +RG GSHAA P+ G D I+A++ IV LQ IVSRN+S L++AVVSVT + +GN+WN++P Sbjct: 182 LIRGQGSHAAYPQEGQDLILASAAIVQNLQQIVSRNISPLRAAVVSVTHIEAGNTWNVLP 241 Query: 252 ETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRWLGGPPPVNNDAGLARLG 311 A LEGTIRTF+ VRS RF +WL ND L + Sbjct: 242 NNARLEGTIRTFDNEVRSLTKRRFSQIIEATAKAYDVELEIKWLMEADLTFNDFELTDVI 301 Query: 312 EETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGT--AGSREWHHPAFNLDEAALP 369 + + P PS AGEDFA YQ+ P +F F+G+ G H + + L Sbjct: 302 RKRTEQWHDKVVYPEPSTAGEDFANYQKQAPSVFAFIGSNEQGGSGLHFADMVVQDETLK 361 Query: 370 VGAAFFAGLAVRVLEHLS 387 V ++ A ++LEH Sbjct: 362 VAVDYYLESAHQLLEHFK 379 >PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 387 Score = 290 bits (741), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 159/379 (41%), Positives = 218/379 (57%), Gaps = 8/379 (2%) Query: 11 AQALNEKVIGIRRHLHQHPELSNEEFETTRYIISLLEQAGITIIDYGLTTGVIAEIGGLQ 70 A+ L ++ RR H +PEL EE T++ + L++ G ++ TGVI + G + Sbjct: 9 AKELQGYIVEKRRDFHMYPELKYEEERTSKIVEEELKKLGYEVVRTA-KTGVIGILKGKE 67 Query: 71 DGPVVALRADIDALPIQEETGLPYASLHPGKSHACGHDFHTAALMGAAYQLKQQEPDLKG 130 DG VALRAD+DALPIQEE +PY S PGK HACGHD HTA L+GAA L + + +L+G Sbjct: 68 DGKTVALRADMDALPIQEENDVPYKSRVPGKMHACGHDAHTAMLLGAAKILAEMKDELQG 127 Query: 131 KVRLLFQPAEEKAKGAQQIIASGALEGVRAVIGMHNKPDLPVGTLGITGGPLMAAADGFV 190 V+L+FQPAEE GA++I+ G L+ V A+ G+H +LP G +GI GPL+A+AD F Sbjct: 128 TVKLIFQPAEEGGLGAKKIVEEGHLDDVDAIFGIHVWAELPSGIIGIKSGPLLASADAFR 187 Query: 191 VEVRGAGSHAAVPEAGTDPIVAASHIVTALQSIVSRNVSALQSAVVSVTKLHSGNSWNII 250 V ++G G H A P DPI A +V A Q I+SR V LQ AV+SVT + +G ++N+I Sbjct: 188 VLIKGKGGHGAAPHLSIDPIALAVDLVNAYQKIISREVDPLQPAVLSVTSIKAGTTFNVI 247 Query: 251 PETAVLEGTIRTFEEAVRSKVLERFXXXXXXXXXXXXXXXXXRW-LGGPPPVNNDAGLAR 309 PE+A + GTIRTF+E VR ++ R + PP N+ LA Sbjct: 248 PESAEILGTIRTFDEEVRDYIVRRMKEITENFANGMRCEGKFELTIEHIPPTINNEKLAN 307 Query: 310 LGEETAATLGFDYLKPSPSPAGEDFAFYQRVVPGLFVFVGTAGSRE-----WHHPAFNLD 364 + LG + +P P+ EDFAFY PGLF+F+G + HHP FN+D Sbjct: 308 FARDVLKVLG-EIREPKPTMGAEDFAFYTTKAPGLFIFLGIRNEEKGIIYPHHHPKFNVD 366 Query: 365 EAALPVGAAFFAGLAVRVL 383 E L +GAA + L L Sbjct: 367 EDILWMGAAIHSLLTYHYL 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,784,196 Number of extensions: 1182696 Number of successful extensions: 2736 Number of sequences better than 1.0e-05: 79 Number of HSP's gapped: 2523 Number of HSP's successfully gapped: 79 Length of query: 394 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 286 Effective length of database: 25,922,359 Effective search space: 7413794674 Effective search space used: 7413794674 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)