BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0028.p (447 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2437 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 561 e-159 BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 548 e-156 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 389 e-108 mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 313 2e-85 mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 302 7e-82 >lin2437 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 561 bits (1445), Expect = e-159, Method: Compositional matrix adjust. Identities = 0/436 (0%), Positives = 0/436 (0%) Query: 3 MTKPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGL 62 M + + +G T + +++ DA + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 YINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 + + + + + S R Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 + + A + + A Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 + H + + + I F+ G +EV + G T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 L + + + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPR 362 Sbjct: 301 360 Query: 363 SSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYESD 422 Sbjct: 361 420 Query: 423 TLRGNLGLPVPANRYA 438 Sbjct: 421 436 >BS_ytnJ [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 548 bits (1413), Expect = e-156, Method: Compositional matrix adjust. Identities = 0/434 (0%), Positives = 0/434 (0%) Query: 3 MTKPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGL 62 M + + +G T + +++ DA + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 YINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 + + + + + S R Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 + + A + + A Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 + H + + + I F+ G +EV + G T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 L + + + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPR 362 Sbjct: 301 360 Query: 363 SSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYESD 422 Sbjct: 361 420 Query: 423 TLRGNLGLPVPANR 436 Sbjct: 421 434 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 0/451 (0%), Positives = 0/451 (0%), Gaps = 19/451 (4%) Query: 7 KQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGLYI-- 64 + L + +A + +L Q + ++ Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 ------NEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVI 118 +A S + +L Sbjct: 63 GLTETYVGN-----WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDS 117 Query: 119 SGGRAGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKE 178 + + + A L ++ +R Sbjct: 118 LKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 179 TGVFFDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFT 238 M L + + + A + Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 239 GHDTLEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIE 298 + + AR F R L + Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 Query: 299 NALNYL-GRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQ 357 E Sbjct: 298 RQPFVASTLLGATTMDQLKTNIESLHLELSE-- 355 Query: 358 AATPRSSF--IGTPEQVADQIQAWF-EXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGL 414 Sbjct: 356 415 Query: 415 FRTEYESDTLRGNLGLPVPANRYAKAPVPSQ 445 Sbjct: 416 446 >mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 313 bits (803), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 0/442 (0%), Positives = 0/442 (0%), Gaps = 13/442 (2%) Query: 8 QLKLGALLHGVGGSTSM-WRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGLYINE 66 ++ V + +A + +L Q + ++ + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 67 K---SIPHFLN-RFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 + + L + VS + D + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 + + + A L L + + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 + + + S + + G T Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 + D ++A Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIK--KNAREKGLTLREVALQAAT 360 Q + L + + + Sbjct: 303 ASTL-LGATTMDQLKTNI--ESLHLELSEDVLAEIEAVHQV 359 Query: 361 PRS--SFIGTPEQVADQIQAWF-EXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRT 417 Sbjct: 360 419 Query: 418 EYESDTLRGNLGLPVPANRYAK 439 Sbjct: 420 441 >mll5137 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 302 bits (774), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 0/434 (0%), Positives = 0/434 (0%), Gaps = 31/434 (7%) Query: 8 QLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGLYINEK 67 + L + +A + +L Q + ++ + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 68 S--IPHFLNR---FEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 ++ A + + R+ + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 ++ N K + + + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAE---------YQRAGK-- 172 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 S + + R + Y+ + Sbjct: 173 ----------IRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEV 222 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 + P + Q + + Sbjct: 223 SQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIAR 282 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPR 362 G + + ++ + ++A Sbjct: 283 RHGLDPAQMALAFVRRQPFVASTLL--GATTMDQLKTNIESLHLELSEDVLAEIEAVHQV 340 Query: 363 SSF--IGTPEQVADQIQAWFEXXXXXXXXXX-XXVPNGLEEFVDRVVPILQERGLFRTEY 419 ++ Sbjct: 341 YTYPA 400 Query: 420 ESDTLRGNLGLPVP 433 Sbjct: 401 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,940,750 Number of extensions: 1470668 Number of successful extensions: 5178 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 5140 Number of HSP's successfully gapped: 27 Length of query: 447 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 337 Effective length of database: 25,691,691 Effective search space: 8658099867 Effective search space used: 8658099867 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)