BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0027.p (411 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH0722 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 281 2e-75 PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 276 4e-74 lin0542 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 268 1e-71 BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 265 1e-70 slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 261 2e-69 >PH0722 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 388 Score = 281 bits (718), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 173/405 (42%), Positives = 230/405 (56%), Gaps = 35/405 (8%) Query: 15 MNFTGLLEPE-----IIAIRRDLHRHPELLYDVARTSAKVAELLETWGIAVRRNVGRHFG 69 MNF L E E II+ RRD H HPEL ++ RTS V E L WG ++R G Sbjct: 6 MNFDPLKEAEKIKDEIISWRRDFHMHPELGFEEERTSKIVEEHLREWGYKIKRA-----G 60 Query: 70 MGVVGTLQGTGGDGPAILLRADMDALPITERNEVPYKSLNAGVMHACGHDAHTAMLLGAA 129 G++ + G G I LRADMDALPI E N+VPYKS G MHACGHDAHTAMLLGAA Sbjct: 61 TGIIADI---GDGGKTIALRADMDALPIQEENDVPYKSRVPGKMHACGHDAHTAMLLGAA 117 Query: 130 KTLAEYRHQLSGKVIFIFQPAEEGAVQSPVDGRLLSGGRDMIEDGILEGVDYCYALHVMP 189 K +AE+ +L KV IFQPAEEG +G MIE G LEGVD + +HV Sbjct: 118 KIIAEHSSELENKVRLIFQPAEEGG----------NGALKMIEAGALEGVDAIFGIHVWA 167 Query: 190 ELPAGTVGVHPHYAMAASSHFSLEFQGTAGHHSAPHRSVDAIQMAARFITEVNALMANQI 249 ELP+G VG+ +A F + G GH +APH S+D I AA + + ++A ++ Sbjct: 168 ELPSGIVGIREGPFLAGVGKFIAKIIGKGGHGAAPHFSIDPIPAAADAVLALQRIVAREV 227 Query: 250 DPSEAAVLAFGTLNAGTAINVIAARSMLTGTFRAFSKPTVAGITEGLKRHAAAIADSSGG 309 DP E+AV+ G + GTA NVI L GTFR F++ + + L+R I +++ Sbjct: 228 DPLESAVVTVGKIQGGTAFNVIPQYVELEGTFRFFTQE----LGKFLERRIREIIENTAK 283 Query: 310 --NWKLELRE---GIAVLNDKEAVQRVLRAAREVLGEEQAVMLNQPSLAGEDFGWYLDRV 364 N K E+ G +ND++ V+ V A+ LG + + + +L GEDF +YL++V Sbjct: 284 AHNCKAEVNTEILGPPTINDEKMVKFVAETAK-ALGLKVGEV--RKTLGGEDFAYYLEKV 340 Query: 365 PGAFAFIGCGNEELGITHALHQPNFNIDEDMLVHGARLLVRLALH 409 PGAF +G NEE GI + H P F++DED+L G L V LA + Sbjct: 341 PGAFIALGIRNEEKGIIYPHHHPKFDVDEDVLYLGTALEVALAFN 385 >PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 387 Score = 276 bits (706), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 158/392 (40%), Positives = 217/392 (55%), Gaps = 19/392 (4%) Query: 21 LEPEIIAIRRDLHRHPELLYDVARTSAKVAELLETWGIAVRRNVGRHFGMGVVGTLQGTG 80 L+ I+ RRD H +PEL Y+ RTS V E L+ G V R GV+G L+G Sbjct: 12 LQGYIVEKRRDFHMYPELKYEEERTSKIVEEELKKLGYEVVRTAK----TGVIGILKGKE 67 Query: 81 GDGPAILLRADMDALPITERNEVPYKSLNAGVMHACGHDAHTAMLLGAAKTLAEYRHQLS 140 DG + LRADMDALPI E N+VPYKS G MHACGHDAHTAMLLGAAK LAE + +L Sbjct: 68 -DGKTVALRADMDALPIQEENDVPYKSRVPGKMHACGHDAHTAMLLGAAKILAEMKDELQ 126 Query: 141 GKVIFIFQPAEEGAVQSPVDGRLLSGGRDMIEDGILEGVDYCYALHVMPELPAGTVGVHP 200 G V IFQPAEEG + G + ++E+G L+ VD + +HV ELP+G +G+ Sbjct: 127 GTVKLIFQPAEEGGL----------GAKKIVEEGHLDDVDAIFGIHVWAELPSGIIGIKS 176 Query: 201 HYAMAASSHFSLEFQGTAGHHSAPHRSVDAIQMAARFITEVNALMANQIDPSEAAVLAFG 260 +A++ F + +G GH +APH S+D I +A + +++ ++DP + AVL+ Sbjct: 177 GPLLASADAFRVLIKGKGGHGAAPHLSIDPIALAVDLVNAYQKIISREVDPLQPAVLSVT 236 Query: 261 TLNAGTAINVIAARSMLTGTFRAFSKPTVAGITEGLKRHAAAIADSSGGNWKLELR-EGI 319 ++ AGT NVI + + GT R F + I +K A+ K EL E I Sbjct: 237 SIKAGTTFNVIPESAEILGTIRTFDEEVRDYIVRRMKEITENFANGMRCEGKFELTIEHI 296 Query: 320 AVLNDKEAVQRVLRAAREVLGEEQAVMLNQPSLAGEDFGWYLDRVPGAFAFIGCGNEELG 379 + E + R +VLGE + +P++ EDF +Y + PG F F+G NEE G Sbjct: 297 PPTINNEKLANFARDVLKVLGE---IREPKPTMGAEDFAFYTTKAPGLFIFLGIRNEEKG 353 Query: 380 ITHALHQPNFNIDEDMLVHGARLLVRLALHEL 411 I + H P FN+DED+L GA + L H L Sbjct: 354 IIYPHHHPKFNVDEDILWMGAAIHSLLTYHYL 385 >lin0542 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 393 Score = 268 bits (686), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 148/392 (37%), Positives = 220/392 (56%), Gaps = 18/392 (4%) Query: 22 EPEIIAIRRDLHRHPELLYDVARTSAKVAELLETWGIAVRRNVGRHFGMGVVGTLQGTGG 81 E +IA RRDLH HPEL + RT+ +VA+ L+ GI RR G++ L+G Sbjct: 13 EENMIAFRRDLHMHPELQWQEFRTTDQVAKQLDQLGIPYRRTNP----TGLIADLKGDKV 68 Query: 82 DGPAILLRADMDALPITERNE-VPYKSLNAGVMHACGHDAHTAMLLGAAKTLAEYRHQLS 140 G I LRADMDALP+ E N+ + YK G MHACGHDAHTAMLL AAK L E + +L Sbjct: 69 -GKTIALRADMDALPVQELNQDLSYKPTEDGKMHACGHDAHTAMLLTAAKALVEIKSELR 127 Query: 141 GKVIFIFQPAEEGAVQSPVDGRLLSGGRDMIEDGILEGVDYCYALHVMPELPAGTVGVHP 200 G V FIFQP+EE + G ++MI G +EGVD+ + +H+ + P+ + Sbjct: 128 GTVRFIFQPSEE----------IAEGAKEMIAQGAMEGVDHVFGIHIWSQTPSNKISCVV 177 Query: 201 HYAMAASSHFSLEFQGTAGHHSAPHRSVDAIQMAARFITEVNALMANQIDPSEAAVLAFG 260 A++ ++F+G GH + PH ++DA +A+ F+ + A++A + DP + V+ G Sbjct: 178 GSTFASADIIQIDFKGQGGHGAMPHDTIDAAVIASSFVMNLQAIVARETDPLDPVVVTIG 237 Query: 261 TLNAGTAINVIAARSMLTGTFRAFSKPTVAGITEGLKRHAAAIADSSGGNWKLELREGIA 320 + GT NVIA + L GT R F+ T A + + ++ +A A GG ++ ++G Sbjct: 238 KMEVGTRYNVIAENARLEGTLRCFNNTTRAKVAKTIEHYAKQTAAIYGGTAEMIYKQGTQ 297 Query: 321 -VLNDKEAVQRVLRAAREVLGEEQAVMLNQPSLAGEDFGWYLDRVPGAFAFIGCGNEELG 379 V+ND+++ V + E GEE + +P+ GEDF ++ D PG+FA +G GN E Sbjct: 298 PVINDEKSALLVQKTIIESFGEEM-LYFERPTTGGEDFSYFQDEAPGSFALVGSGNPEKD 356 Query: 380 ITHALHQPNFNIDEDMLVHGARLLVRLALHEL 411 A H FNIDE ++ +GA L + A + L Sbjct: 357 TEWAHHHGRFNIDESVMKNGAELYAQFAYNYL 388 >BH1613 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 404 Score = 265 bits (677), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 162/386 (41%), Positives = 220/386 (56%), Gaps = 15/386 (3%) Query: 24 EIIAIRRDLHRHPELLYDVARTSAKVAELLETWGIAVRRNVGRHFGMGVVGTLQGTGGDG 83 E++ IRR LH+HPEL ++ +T A +A+ LE G+ VRR VG GVVGT++G G G Sbjct: 15 ELVDIRRTLHKHPELSFEEVKTPAFIADYLEKLGVNVRRGVGG---RGVVGTIKG-GKPG 70 Query: 84 PAILLRADMDALPITERNEVPYKSLNAGVMHACGHDAHTAMLLGAAKTLAEYRHQLSGKV 143 P + LRAD DALPI E +PY+S GVMHACGHD HTA LL AK L E+R QL G V Sbjct: 71 PTVALRADFDALPIQEETGLPYQSTVPGVMHACGHDGHTATLLIIAKILMEHRQQLEGSV 130 Query: 144 IFIFQPAEEGAVQSPVDGRLLSGGRDMIEDGILEGVDYCYALHVMPELPAGTVGVHPHYA 203 + I Q AEE A G MIEDG L+GVD Y H+ +PAG +G Sbjct: 131 VLIHQFAEELAP---------GGAIAMIEDGCLDGVDVIYGTHLWATMPAGEIGYRSGAF 181 Query: 204 MAASSHFSLEFQGTAGHHSAPHRSVDAIQMAARFITEVNALMANQIDPSEAAVLAFGTLN 263 MAA+ F++ G GH +APH +VDA+ + A + ++ +++ +DP E+AV+ + Sbjct: 182 MAAADKFAVTIYGKGGHGAAPHETVDAVTVGASVVQQLQQIVSRNVDPLESAVVTVASFQ 241 Query: 264 AGTAINVIAARSMLTGTFRAFSKPTVAGITEGLKRHAAAIADSSGGNWKLELREGI-AVL 322 AG NVIA ++ L GT R F + I E ++R + D++G + +G AV+ Sbjct: 242 AGGPFNVIADKANLVGTVRTFKEDIQDMIIERMERTIKGVCDAAGATYAFAYDKGYPAVI 301 Query: 323 NDKEAVQRVLRAAREVLGEEQAVMLNQPSLAGEDFGWYLDRVPGAFAFIGCGNEELGITH 382 N + + AA V+GEE + P + GEDF +YL VPGAF F G GNE+ GI + Sbjct: 302 NHPKETDDLKNAAVAVVGEEHVAEM-APVMGGEDFAYYLHHVPGAFFFTGAGNEDAGIIY 360 Query: 383 ALHQPNFNIDEDMLVHGARLLVRLAL 408 H P F IDE L A+ L+ L Sbjct: 361 PHHHPKFMIDERSLAIAAKTLLTATL 386 >slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 261 bits (667), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 150/387 (38%), Positives = 217/387 (56%), Gaps = 21/387 (5%) Query: 24 EIIAIRRDLHRHPELLYDVARTSAKVAELLETWGIAVRRNVGRHFGMGVVGTLQGTGGDG 83 ++I RR H++PEL + T+A +AE L I + + G++ T+ +G G Sbjct: 38 QLIQWRRQFHQYPELGFQEQLTAAHIAETLTKLEIPHTPGIAK---TGIMATVD-SGKPG 93 Query: 84 PAILLRADMDALPITERNEVPYKSLNAGVMHACGHDAHTAMLLGAAKTLAEYRHQLSGKV 143 P + +RADMDALP+TE NEV Y+SL+ G MHACGHD HTA+ LG A+ LA +R G+V Sbjct: 94 PVLAIRADMDALPVTEENEVDYRSLHPGKMHACGHDGHTAIALGTAQYLAAHRDSFRGQV 153 Query: 144 IFIFQPAEEGAVQSPVDGRLLSGGRDMIEDGILE--GVDYCYALHVMPELPAGTVGVHPH 201 F FQPAEEG G + MIE G+LE VD LH+ +LP GTVG+ P Sbjct: 154 KFFFQPAEEGP----------GGAKPMIEAGVLENPAVDAIVGLHLWNDLPVGTVGIKPG 203 Query: 202 YAMAASSHFSLEFQGTAGHHSAPHRSVDAIQMAARFITEVNALMANQIDPSEAAVLAFGT 261 MAA HF + G GH + PH++VD + ++A+ + + ++A ++P ++AV+ G Sbjct: 204 PVMAAVEHFECQLFGQGGHGAMPHQTVDTLVISAQIVMALQGIVARNLNPLQSAVVTVGQ 263 Query: 262 LNAGTAINVIAARSMLTGTFRAFSKPTVAG-ITEGLKRHAAAIADSSGGNWKLELREGI- 319 L +GTA NVI + GT R F P+ AG + ++ I S G N++ E I Sbjct: 264 LQSGTAFNVIPDSAYFRGTVRYFD-PSFAGYFAQRIEEIIKGICQSHGANYQFTY-ENIY 321 Query: 320 -AVLNDKEAVQRVLRAAREVLGEEQAVMLNQPSLAGEDFGWYLDRVPGAFAFIGCGNEEL 378 V+ND+ V AA +VL + + + +LAGED ++L VPG + F+G N +L Sbjct: 322 PPVVNDRRLADLVRSAAADVLLTDDHLQPDYQTLAGEDMSFFLQAVPGCYFFLGSANGDL 381 Query: 379 GITHALHQPNFNIDEDMLVHGARLLVR 405 G+ + H P FN DE +L G L VR Sbjct: 382 GLAYPHHHPRFNFDEAVLPVGVELFVR 408 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,799,929 Number of extensions: 1385175 Number of successful extensions: 4312 Number of sequences better than 1.0e-05: 74 Number of HSP's gapped: 4078 Number of HSP's successfully gapped: 74 Length of query: 411 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 302 Effective length of database: 25,807,025 Effective search space: 7793721550 Effective search space used: 7793721550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)